Emily Elizabeth Wear

Works (12)

Updated: August 30th, 2025 05:03

2025 article

A comparison of genomic methods to assess DNA replication timing

Wheeler, E., Mickelson-Young, L., Wear, E. E., Burroughs, M., Bass, H. W., Concia, L., … Hanley-Bowdoin, L. (2025, May 22). Scientific Reports, Vol. 15.

By: E. Wheeler*, L. Mickelson-Young n, E. Wear n, M. Burroughs n, H. Bass*, L. Concia, W. Thompson n, L. Hanley-Bowdoin n

MeSH headings : DNA Replication Timing; Genomics / methods; Humans; DNA Replication; S Phase / genetics; Deoxyuridine / analogs & derivatives; G1 Phase / genetics; Cell Nucleus / genetics
topics (OpenAlex): DNA Repair Mechanisms; Cancer Genomics and Diagnostics; Genomics and Chromatin Dynamics
Sources: Web Of Science, ORCID, NC State University Libraries
Added: May 22, 2025

2025 article

Comparison of transcriptional activity profiling by metabolic labeling or nuclear RNA sequencing

Wear, E. E., Mickelson‐Young, L., Bass, H. W., Hanley‐Bowdoin, L., Thompson, W. F., & Concia, L. (2025, August 1). The Plant Journal, Vol. 123.

By: E. Wear n, L. Mickelson‐Young n, H. Bass*, L. Hanley‐Bowdoin n, W. Thompson n & L. Concia

author keywords: transcriptional activity; nuclear RNA; metabolic labeling; 5-ethynyl uridine; newly synthesized RNA; <fixed-case><italic>Zea mays</italic></fixed-case>; maize; introns; non-coding genes; cellular RNA
topics (OpenAlex): RNA modifications and cancer; Genomics and Chromatin Dynamics; Bioinformatics and Genomic Networks
Sources: Web Of Science, NC State University Libraries
Added: August 25, 2025

2020 article

Arabidopsis DNA Replication Initiates in Intergenic, AT-Rich Open Chromatin

Wheeler, E., Brooks, A. M., Concia, L., Vera, D. L., Wear, E. E., LeBlanc, C., … Hanley-Bowdoin, L. (2020, March 23). PLANT PHYSIOLOGY, Vol. 183, pp. 206–220.

By: E. Wheeler n, A. Brooks n, L. Concia n, D. Vera*, E. Wear n, C. LeBlanc*, U. Ramu*, M. Vaughn* ...

MeSH headings : Arabidopsis / genetics; Arabidopsis / metabolism; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Chromatin / metabolism; DNA Replication / genetics; DNA Replication / physiology; DNA, Plant / metabolism; DNA, Plant / physiology; Replication Origin / genetics; Replication Origin / physiology
topics (OpenAlex): Genomics and Chromatin Dynamics; Chromosomal and Genetic Variations; DNA Repair Mechanisms
TL;DR: A new functional assay to label and map very early replicating loci that must, by definition, include at least a subset of replication origins, and indicates that IRs are associated with accessible chromatin. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: June 15, 2020

2020 article

Comparing DNA replication programs reveals large timing shifts at centromeres of endocycling cells in maize roots

Wear, E. E., Song, J., Zynda, G. J., Mickelson-Young, L., LeBlanc, C., Lee, T.-J., … Thompson, W. F. (2020, October 14). PLoS Genetics, Vol. 16.

By: E. Wear n, J. Song*, G. Zynda*, L. Mickelson-Young n, C. LeBlanc*, T. Lee n, D. Deppong n, G. Allen n ...

MeSH headings : Cell Nucleus / drug effects; Cell Nucleus / genetics; Centromere / drug effects; Centromere / genetics; DNA Replication / drug effects; DNA Replication / genetics; DNA Replication Timing / drug effects; DNA Replication Timing / genetics; DNA, Plant / drug effects; DNA, Plant / genetics; Deoxyuridine / analogs & derivatives; Deoxyuridine / pharmacology; Endocytosis / drug effects; Meristem / drug effects; Meristem / genetics; Mitosis / drug effects; Mitosis / genetics; Nucleosomes / drug effects; Plant Roots / drug effects; Plant Roots / genetics; Plant Roots / growth & development; S Phase / genetics; Zea mays / genetics; Zea mays / growth & development
topics (OpenAlex): Chromosomal and Genetic Variations; Plant Molecular Biology Research; Plant nutrient uptake and metabolism
Sources: Web Of Science, NC State University Libraries
Added: November 9, 2020

2020 article

Loss of Small-RNA-Directed DNA Methylation in the Plant Cell Cycle Promotes Germline Reprogramming and Somaclonal Variation

Borges, F., Donoghue, M. T. A., LeBlanc, C., Wear, E. E., Tanurdžić, M., Berube, B., … Martienssen, R. A. (2020, December 3). Current Biology, Vol. 31, pp. 591-+.

MeSH headings : Arabidopsis / genetics; Arabidopsis / metabolism; Cell Cycle; Cytosine; DNA Methylation; DNA, Plant; Gene Expression Regulation, Plant; Germ Cells / metabolism; Histones / genetics; Histones / metabolism; Plants / metabolism; RNA, Small Interfering / genetics
topics (OpenAlex): Plant Molecular Biology Research; Plant nutrient uptake and metabolism; Plant responses to water stress
TL;DR: Comparative analysis with methylomes of various tissues and cell types suggests that loss of small-RNA-directed non-CG methylation during DNA replication promotes germline reprogramming and epigenetic variation in plants propagated as clones. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, NC State University Libraries
Added: December 4, 2020

2018 article

Chromatin structure profile data from DNS-seq: Differential nuclease sensitivity mapping of four reference tissues of B73 maize (Zea mays L)

Turpin, Z. M., Vera, D. L., Savadel, S. D., Lung, P.-Y., Wear, E. E., Mickelson-Young, L., … Bass, H. W. (2018, August 9). Data in Brief, Vol. 20, pp. 358–363.

topics (OpenAlex): Genomics and Phylogenetic Studies; Molecular Biology Techniques and Applications; Chromosomal and Genetic Variations
TL;DR: Data from Next-Generation Sequencing of differential micrococcal nuclease digestions of formaldehyde-crosslinked chromatin in selected tissues of maize (Zea mays) inbred line B73 are presented. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: December 3, 2018

2018 article

Genome-Wide Analysis of the Arabidopsis Replication Timing Program

Concia, L., Brooks, A. M., Wheeler, E., Zynda, G. J., Wear, E. E., LeBlanc, C., … Hanley-Bowdoin, L. (2018, January 4). PLANT PHYSIOLOGY.

By: L. Concia n, A. Brooks n, E. Wheeler n, G. Zynda, E. Wear n, C. LeBlanc*, J. Song, T. Lee n ...

MeSH headings : Arabidopsis / genetics; Chromatin / genetics; Chromatin / metabolism; Chromosomes, Plant; DNA Replication Timing; DNA Transposable Elements; Flow Cytometry; Genome, Plant; Genome-Wide Association Study; S Phase / genetics; Sequence Analysis, DNA / methods
topics (OpenAlex): Genomics and Chromatin Dynamics; Chromosomal and Genetic Variations; DNA Repair Mechanisms
TL;DR: The thymidine analog, 5-ethynyl-2′-deoxyuridine, is used in combination with flow sorting and Repli-Seq to describe the genome-wide replication timing program for Arabidopsis (Arabidopsis thaliana) Col-0 suspension cells, and genomic regions that replicate predominantly during early, mid, and late S phase are identified. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2017 article

Genomic Analysis of the DNA Replication Timing Program during Mitotic S Phase in Maize (Zea mays) Root Tips

Wear, E. E., Song, J., Zynda, G. J., LeBlanc, C., Lee, T.-J., Mickelson-Young, L., … Thompson, W. F. (2017, August 25). The Plant Cell, Vol. 29, pp. 2126–2149.

MeSH headings : Base Sequence; Chromosomes, Plant / genetics; DNA Replication Timing / genetics; DNA Transposable Elements / genetics; Genes, Plant; Genomics; Meristem / cytology; Meristem / genetics; Mitosis / genetics; Models, Genetic; S Phase / genetics; Tandem Repeat Sequences / genetics; Time Factors; Transcription, Genetic; Zea mays / cytology; Zea mays / genetics
topics (OpenAlex): Chromosomal and Genetic Variations; Genomics and Chromatin Dynamics; Genomics and Phylogenetic Studies
TL;DR: A novel adaptation of the “Repli-seq” assay for use in intact root tips of maize (Zea mays) that includes several different cell lineages and present whole-genome replication timing profiles from cells in early, mid, and late S phase of the mitotic cell cycle is reported. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2017 article

Repliscan: a tool for classifying replication timing regions

Zynda, G. J., Song, J., Concia, L., Wear, E. E., Hanley-Bowdoin, L., Thompson, W. F., & Vaughn, M. W. (2017, August 7). BMC Bioinformatics, Vol. 18, pp. 1–14.

By: G. Zynda, J. Song, L. Concia n, E. Wear n, L. Hanley-Bowdoin n, W. Thompson n, M. Vaughn

author keywords: DNA replication; Repli-seq; Classification
MeSH headings : DNA Replication Timing; Genome; Genome Size; High-Throughput Nucleotide Sequencing / methods; Sequence Analysis, DNA / methods; Software
topics (OpenAlex): Genomics and Phylogenetic Studies; Genomics and Chromatin Dynamics; Chromosomal and Genetic Variations
TL;DR: To accurately detect and classify regions of replication across the genome, Repliscan is presented, which robustly normalizes, automatically removes outlying and uninformative data points, and classifies Repli-seq signals into discrete combinations of replication signatures. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2016 article

A flow cytometric method for estimating S-phase duration in plants

Mickelson-Young, L., Wear, E., Mulvaney, P., Lee, T.-J., Szymanski, E. S., Allen, G., … Thompson, W. (2016, October 3). Journal of Experimental Botany, Vol. 11, pp. 6077–6087.

author keywords: Arabidopsis; barley; EdU; flow cytometry; maize; replication timing; rice; S-phase duration; wheat
MeSH headings : Arabidopsis / cytology; Arabidopsis / growth & development; DNA, Plant / metabolism; Deoxyuridine / analogs & derivatives; Deoxyuridine / metabolism; Flow Cytometry / methods; G1 Phase / physiology; G2 Phase / physiology; Hordeum / cytology; Hordeum / growth & development; Meristem / cytology; Meristem / growth & development; Oryza / cytology; Oryza / growth & development; S Phase / physiology; Triticum / cytology; Triticum / growth & development; Zea mays / cytology; Zea mays / growth & development
topics (OpenAlex): Chromosomal and Genetic Variations; Plant nutrient uptake and metabolism; Plant Genetic and Mutation Studies
TL;DR: This work estimated S-phase duration for several plant species by following EdU-labeled nuclei from G1 to G2 using bivariate flow cytometry, finding that S- phase duration is relatively consistent over a range of genome sizes. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2015 article

Defining multiple, distinct, and shared spatiotemporal patterns of DNA replication and endoreduplication from 3D image analysis of developing maize (Zea mays L.) root tip nuclei

Bass, H. W., Hoffman, G. G., Lee, T.-J., Wear, E. E., Joseph, S. R., Allen, G. C., … Thompson, W. F. (2015, September 22). Plant Molecular Biology, Vol. 89, pp. 339–351.

By: H. Bass*, G. Hoffman*, T. Lee n, E. Wear n, S. Joseph*, G. Allen n, L. Hanley-Bowdoin n, W. Thompson n

author keywords: Cell cycle; DNA synthesis; Grass
MeSH headings : Cell Nucleus / genetics; Cell Nucleus / metabolism; DNA Replication / genetics; DNA Replication Timing / genetics; DNA, Plant / biosynthesis; DNA, Plant / genetics; Endoreduplication / genetics; Genes, Plant; Imaging, Three-Dimensional; Meristem / genetics; Meristem / growth & development; Meristem / metabolism; Models, Biological; S Phase / genetics; Zea mays / genetics; Zea mays / growth & development; Zea mays / metabolism
topics (OpenAlex): Chromosomal and Genetic Variations; Genomics and Chromatin Dynamics; DNA Repair Mechanisms
TL;DR: 3D quantitative analysis of DNA replication and endoreduplication in nuclei from pulse-labeled developing maize root tips revealed that maize euchromatin exists as an intermingled mixture of two components distinguished by their condensation state and replication timing. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2008 article

Invasion of a West Everglades wetland by Melaleuca quinquenervia countered by classical biological control

Tipping, P. W., Martin, M. R., Nimmo, K. R., Pierce, R. M., Smart, M. D., White, E., & Madeira, P. T. (2008, September 7). Biological Control.

By: P. Tipping*, M. Martin*, K. Nimmo*, R. Pierce*, M. Smart*, E. White n, P. Madeira*

author keywords: Weed biological control; Melaleuca; Florida Everglades; Community level impacts
topics (OpenAlex): Biological Control of Invasive Species; Ecology and Vegetation Dynamics Studies; Plant and animal studies
TL;DR: This is the first study with controls that quantifies the population level regulation ofmelaleuca by introduced biological control agents and corroborates other correlative studies that documented significant changes in melaleuca communities after the introduction and establishment of biological controlagents. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Source: Web Of Science
Added: August 6, 2018

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