Prasenjit Sarkar

Works (6)

Updated: July 5th, 2023 15:46

2016 journal article

Identification of Epigenetic Factor Proteins Expressed in Human Embryonic Stem Cell-Derived Trophoblasts and in Human Placental Trophoblasts

JOURNAL OF PROTEOME RESEARCH, 15(8), 2433–2444.

By: P. Sarkar n, A. Mischler n, S. Randall n, T. Collier n, K. Dorman*, K. Boggess*, D. Muddiman n , B. Rao n 

Contributors: P. Sarkar n, A. Mischler n, S. Randall n, T. Collier n, K. Dorman*, K. Boggess*, D. Muddiman n , B. Rao n 

author keywords: quantitative mass spectrometry; SILAC; human embryonic stem cell; trophoblast; epigenetic factor
MeSH headings : Cell Differentiation; DNA (Cytosine-5-)-Methyltransferases / genetics; Epigenomics; Female; Gene Expression / genetics; Human Embryonic Stem Cells / cytology; Humans; Placenta / chemistry; Placenta / cytology; Pregnancy; Transcription Factors / genetics; Trophoblasts / chemistry; Trophoblasts / cytology
TL;DR: The results support the use of hESC-derived trophoblasts as a model for placental troPHoblasts, which will enable further investigation of epigenetic factors involved in human trophoblast development, and hypothesized that nuclear proteomics of h ESPCs may be used for screening epigenetic factor proteins expressed by primary trophblasts in human placental tissue. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2015 journal article

Activin/Nodal Signaling Switches the Terminal Fate of Human Embryonic Stem Cell-derived Trophoblasts

JOURNAL OF BIOLOGICAL CHEMISTRY, 290(14), 8834–8848.

By: P. Sarkar*, S. Randall*, T. Collier *, A. Nero, T. Russell n, D. Muddiman* , B. Rao* 

Contributors: P. Sarkar*, S. Randall*, T. Collier *, A. Nero, T. Russell n, D. Muddiman* , B. Rao* 

MeSH headings : Activins / metabolism; Base Sequence; Cell Differentiation; Cell Lineage; DNA Methylation; DNA Primers; Embryonic Stem Cells / cytology; Embryonic Stem Cells / metabolism; Ephrin-B2 / genetics; Humans; Nodal Protein / metabolism; Polymerase Chain Reaction; Promoter Regions, Genetic; Signal Transduction; Trophoblasts / cytology; Trophoblasts / metabolism
TL;DR: It is shown that activin/nodal signaling switches the terminal fate of these hESC-derived trophoblasts, providing a model system that allows for targeted derivation of extravillous trophoblast and syncytiotrophoblasts. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2013 review

Trophoblast differentiation of human embryonic stem cells

[Review of ]. BIOTECHNOLOGY JOURNAL, 8(4), 421-+.

By: K. Tiruthani  n, P. Sarkar n & B. Rao n 

Contributors: K. Tiruthani  n, P. Sarkar n & B. Rao n 

author keywords: Bone morphogenetic protein; CDX2; ELF5; hESC; Trophoblast
MeSH headings : Animals; Bone Morphogenetic Proteins / pharmacology; Cell Culture Techniques; Cell Differentiation / drug effects; Cell Differentiation / physiology; Embryonic Stem Cells / cytology; Embryonic Stem Cells / drug effects; Humans; Trophoblasts / cytology; Trophoblasts / drug effects
TL;DR: An overview of previously described efforts to obtain trophoblasts from hESCs is provided and the merits and limitations of hESC‐derived cells as a source of trophoblast derivatives are discussed. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2012 journal article

Targeted Proteomics of the Secretory Pathway Reveals the Secretome of Mouse Embryonic Fibroblasts and Human Embryonic Stem Cells

MOLECULAR & CELLULAR PROTEOMICS, 11(12), 1829–1839.

By: P. Sarkar n, S. Randall n, D. Muddiman n  & B. Rao n 

Contributors: P. Sarkar n, S. Randall n, D. Muddiman n  & B. Rao n 

MeSH headings : Animals; Cell Differentiation; Cells, Cultured; Culture Media, Conditioned / analysis; Embryonic Stem Cells / cytology; Embryonic Stem Cells / metabolism; Fibroblasts / cytology; Fibroblasts / metabolism; Humans; Mice; Proteins / analysis; Secretory Pathway; Subcellular Fractions / chemistry
TL;DR: Targeted proteomics of secretory pathway organelles is shown to be a powerful alternate approach for interrogating the cellular secretome and expected to be particularly valuable in two contexts highly relevant to hESC biology: obtaining a temporal snapshot of proteins secreted in response to a differentiation trigger, and identifying proteinssecreted by cells that are isolated from a heterogeneous population. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2011 journal article

Comparison of stable-isotope labeling with amino acids in cell culture and spectral counting for relative quantification of protein expression

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 25(17), 2524–2532.

By: T. Collier n, S. Randall n, P. Sarkar n, B. Rao n , R. Dean n  & D. Muddiman n 

Contributors: T. Collier n, S. Randall n, P. Sarkar n, B. Rao n , R. Dean n  & D. Muddiman n 

MeSH headings : Amino Acid Sequence; Amino Acids / chemistry; Amino Acids / metabolism; Animals; Cell Differentiation; Cell Line; Ectoderm; Embryonic Stem Cells / cytology; Embryonic Stem Cells / metabolism; Humans; Isotope Labeling / methods; Mass Spectrometry; Mice; Molecular Sequence Data; Peptide Fragments; Peptide Mapping; Proteins / analysis; Proteins / chemistry; Proteins / metabolism; Proteomics / methods; Sensitivity and Specificity; Tandem Mass Spectrometry; Time Factors
TL;DR: The comparison of stable-isotope labeling of amino acids in cell culture (SILAC) with spectral counting for the quantification of human embryonic stem cells as they differentiate toward the trophectoderm at three time points is presented. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2011 journal article

The subcellular proteome of undifferentiated human embryonic stem cells

PROTEOMICS, 12(3), 421–430.

By: P. Sarkar n, T. Collier n, S. Randall n, D. Muddiman n  & B. Rao n 

Contributors: P. Sarkar n, T. Collier n, S. Randall n, D. Muddiman n  & B. Rao n 

author keywords: Cell biology; Extracellular proteins; Human embryonic stem cells; Subcellular fractionation; Subcellular proteomics
MeSH headings : Animals; Cell Differentiation; Cells, Cultured; Culture Media, Conditioned; Cytosol / metabolism; Embryonic Stem Cells / cytology; Embryonic Stem Cells / metabolism; Endoplasmic Reticulum / genetics; Endoplasmic Reticulum / metabolism; Gene Expression Regulation, Developmental; Golgi Apparatus / genetics; Golgi Apparatus / metabolism; Humans; Membrane Proteins / genetics; Membrane Proteins / metabolism; Mice; Nuclear Proteins / genetics; Nuclear Proteins / metabolism; Signal Transduction / genetics; Subcellular Fractions / metabolism
TL;DR: The results show that systematic subcellular proteome analysis is a valuable tool for studying hESC biology and shows that proteomic analysis of the endoplasmic reticulum and Golgi compartments is a powerful alternative approach to identify secreted proteins since these are synthesized in the ER and transit through the Golgi. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

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