Steven C. Huber

Works (50)

Updated: July 5th, 2023 16:04

2008 journal article

Sequential transphosphorylation of the BRI1/BAK1 receptor kinase complex impacts early events in brassinosteroid signaling

DEVELOPMENTAL CELL, 15(2), 220–235.

By: X. Wang n, U. Kota n, K. He *, K. Blackburn n, J. Li *, M. Goshe n, S. Huber*, S. Clouse n

MeSH headings : Amino Acid Sequence; Arabidopsis / drug effects; Arabidopsis / enzymology; Arabidopsis Proteins / chemistry; Arabidopsis Proteins / metabolism; Brassinosteroids; Cholestanols / metabolism; Cholestanols / pharmacology; Chromatography, Liquid; Flagellin / metabolism; Mass Spectrometry; Models, Biological; Molecular Sequence Data; Mutation / genetics; Phosphorylation / drug effects; Protein Kinases / chemistry; Protein Kinases / metabolism; Protein Serine-Threonine Kinases / chemistry; Protein Serine-Threonine Kinases / metabolism; Protein Structure, Tertiary; Signal Transduction / drug effects; Steroids, Heterocyclic / metabolism; Steroids, Heterocyclic / pharmacology; Substrate Specificity / drug effects
TL;DR: A sequential transphosphorylation model in which BRI1 controls signaling specificity by direct BR binding followed by substrate phosphorylation is proposed, which suggests both conservation and distinct differences between the molecular mechanisms regulating phosphorylated-dependent kinase activation in plant and animal receptor kinases. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2005 journal article

Free amino acid profiles suggest a possible role for asparagine in the control of storage-product accumulation in developing seeds of low- and high-protein soybean lines

JOURNAL OF EXPERIMENTAL BOTANY, 56(417), 1951–1963.

By: C. Hernandez-Sebastia*, F. Marsolais *, C. Saravitz n, D. Israel n, R. Dewey n & S. Huber*

author keywords: amino acid profile; carbon partitioning; seed nitrogen supply; seed protein content; soybean
MeSH headings : Amino Acids / metabolism; Asparagine / physiology; Cotyledon / physiology; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; Light; Plant Oils / metabolism; Plant Proteins / biosynthesis; Seeds / physiology; Seeds / radiation effects; Soybeans / embryology; Soybeans / metabolism; Soybeans / radiation effects; Transcription, Genetic
TL;DR: The hypothesis that high seed-protein content in soybean is determined by the capacity of the embryo to take up nitrogen sources and to synthesize storage proteins is supported. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2005 journal article

Identification and functional analysis of in vivo phosphorylation sites of the Arabidopsis BRASSINOSTEROID-INSENSITIVE1 receptor kinase

PLANT CELL, 17(6), 1685–1703.

By: X. Wang n, M. Goshe n, E. Soderblom n, B. Phinney *, J. Kuchar*, J. Li *, T. Asami *, S. Yoshida *, S. Huber*, S. Clouse n

MeSH headings : Arabidopsis / enzymology; Arabidopsis / genetics; Arabidopsis Proteins / chemistry; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Binding Sites / physiology; Cell Membrane / metabolism; Conserved Sequence / genetics; Mutation / genetics; Phosphorylation; Protein Kinases / chemistry; Protein Kinases / genetics; Protein Kinases / metabolism; Protein Serine-Threonine Kinases / chemistry; Protein Serine-Threonine Kinases / genetics; Protein Serine-Threonine Kinases / metabolism; Protein Structure, Tertiary / physiology; Serine / chemistry; Serine / metabolism; Signal Transduction / physiology; Threonine / chemistry; Threonine / metabolism
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2005 journal article

Oleate desaturase enzymes of soybean: evidence of regulation through differential stability and phosphorylation

PLANT JOURNAL, 44(3), 433–446.

By: G. Tang n, W. Novitzky n, H. Griffin n, S. Huber* & R. Dewey n

author keywords: oleate desaturase; FAD2; protein phosphorylation; 26S proteasome; polyunsaturated fatty acids; soybean (Glycine max)
MeSH headings : Amino Acid Sequence; Enzyme Stability; Fatty Acid Desaturases / chemistry; Fatty Acid Desaturases / genetics; Fatty Acid Desaturases / metabolism; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Hot Temperature; Isoenzymes / genetics; Isoenzymes / metabolism; Molecular Sequence Data; Oxidoreductases Acting on CH-CH Group Donors / chemistry; Oxidoreductases Acting on CH-CH Group Donors / genetics; Oxidoreductases Acting on CH-CH Group Donors / metabolism; Phosphorylation; Saccharomyces cerevisiae / genetics; Sequence Homology, Amino Acid; Soybeans / enzymology; Soybeans / genetics
TL;DR: Results show that post-translational regulatory mechanisms are likely to play an important role in modulating FAD2-1 enzyme activities. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2004 journal article

Transgenic cotton with improved resistance to glyphosate herbicide

Crop Science, 44(1), 234–240.

By: O. May*, A. Culpepper*, R. Cerny*, C. Coots*, C. Corkern*, J. Cothren*, K. Croon*, K. Ferreira* ...

Source: NC State University Libraries
Added: August 6, 2018

2003 journal article

A novel C-terminal proteolytic processing of cytosolic pyruvate kinase, its phosphorylation and degradation by the proteasome in developing soybean seeds

PLANT JOURNAL, 34(1), 77–93.

By: G. Tang n, S. Hardin n, R. Dewey n & S. Huber n

author keywords: pyruvate kinase; phosphorylation site; C-terminal truncation; degradation; proteasome; soybean (Glycine max (L) cv Ransom)
MeSH headings : Amino Acid Sequence; Cytosol / enzymology; Molecular Weight; Peptide Hydrolases / metabolism; Phosphorylation; Phylogeny; Proteasome Endopeptidase Complex; Protein Processing, Post-Translational; Protein Subunits / chemistry; Protein Subunits / metabolism; Pyruvate Kinase / chemistry; Pyruvate Kinase / metabolism; Seeds / enzymology; Seeds / metabolism; Soybeans / enzymology; Soybeans / metabolism
TL;DR: The results suggest that there are two pathways for PyrKinc modification at the post-translational level: one involves partial C-terminal truncation to generate a 51 kDa pyruvate kinase subunit which might have altered regulatory properties and the other involves phosphorylation and ubiquitin conjugation that targets the protein to the 26S proteasome for complete degradation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2003 journal article

The C-terminal tail of Arabidopsis 14-3-3 omega functions as an autoinhibitor and may contain a tenth alpha-helix

PLANT JOURNAL, 34(4), 473–484.

By: W. Shen  n, A. Clark n & S. Huber n

author keywords: 14-3-3 proteins; nitrate reductase; autoinhibition; protein structure; protein-protein interaction; protein phosphorylation
MeSH headings : 14-3-3 Proteins; Amino Acid Sequence; Antibodies / immunology; Arabidopsis / metabolism; Arabidopsis Proteins / antagonists & inhibitors; Arabidopsis Proteins / chemistry; Arabidopsis Proteins / metabolism; Arabidopsis Proteins / pharmacology; Cations / pharmacology; Molecular Sequence Data; Protein Binding / drug effects; Protein Isoforms / chemistry; Protein Isoforms / metabolism; Protein Structure, Secondary; Tyrosine 3-Monooxygenase / antagonists & inhibitors; Tyrosine 3-Monooxygenase / chemistry; Tyrosine 3-Monooxygenase / metabolism; Tyrosine 3-Monooxygenase / pharmacology
TL;DR: It is proposed that in the absence of polycations, access of target proteins to their binding groove in the 14-3-3 protein is restricted by the C-terminus, which acts as part of a gate that opens with the binding of poly cations to loop 8. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2002 journal article

Divalent cations and polyamines bind to loop 8 of 14-3-3 proteins, modulating their interaction with phosphorylated nitrate reductase

PLANT JOURNAL, 29(2), 119–129.

By: G. Athwal n & S. Huber n

author keywords: 14-3-3 proteins; nitrate reductase; polyamines; cation-binding site; site-directed mutagenesis; truncated proteins
MeSH headings : 14-3-3 Proteins; Arabidopsis Proteins; Binding Sites / genetics; Calcium-Binding Proteins / metabolism; Cations, Divalent / metabolism; Cations, Divalent / pharmacology; Magnesium / metabolism; Magnesium / pharmacology; Mutagenesis, Site-Directed; Mutation; Nitrate Reductase; Nitrate Reductases / antagonists & inhibitors; Nitrate Reductases / metabolism; Phosphorylation / drug effects; Plant Proteins / metabolism; Polyamines / metabolism; Polyamines / pharmacology; Protein Binding; Protein Conformation; Spermidine / pharmacology; Spermine / pharmacology; Tyrosine 3-Monooxygenase / chemistry; Tyrosine 3-Monooxygenase / genetics; Tyrosine 3-Monooxygenase / metabolism
TL;DR: It is reported that micromolar concentrations of the polyamines, spermidine(4+) and spermine(3+), can substitute for divalent cations in modulating 14-3-3 action and proposed that binding of polyamines to 14- 3-3s could be involved in their regulation of plant growth and development. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2002 journal article

Gravity-stimulated changes in auxin and invertase gene expression in maize pulvinal cells

PLANT PHYSIOLOGY, 128(2), 591–602.

By: J. Long, W. Zhao*, A. Rashotte *, G. Muday * & S. Huber n

MeSH headings : Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Glycoside Hydrolases / genetics; Glycoside Hydrolases / metabolism; Gravitropism / physiology; Gravity Sensing / physiology; Hexoses / metabolism; Indoleacetic Acids / metabolism; Phthalimides / pharmacology; Plant Growth Regulators / pharmacology; Potassium / metabolism; Pulvinus / drug effects; Pulvinus / genetics; Pulvinus / physiology; Zea mays / drug effects; Zea mays / genetics; Zea mays / physiology; beta-Fructofuranosidase
TL;DR: A two-phase model describing possible relationships between gravitropic curvature, IAA redistribution, and Ivr2 expression is presented and indicates that auxin redistribution, as a result of polar auxin transport, is necessary for gravity-stimulatedIvr2 transcript accumulation and differential cell elongation across the maize pulvinus. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2002 journal article

Metabolic enzymes as targets for 14-3-3 proteins

PLANT MOLECULAR BIOLOGY, 50(6), 1053–1063.

By: S. Huber n, C. MacKintosh * & W. Kaiser*

author keywords: 14-3-3-affinity chromatography; divalent cations; glutamine synthetase; NADH : nitrate reductase; polyamines; proteolytic degradation; sucrose-phosphate synthase
MeSH headings : 14-3-3 Proteins; Adenosine Monophosphate / pharmacology; Amino Acid Oxidoreductases / metabolism; Enzymes / metabolism; Glucosyltransferases / metabolism; Glutamate-Ammonia Ligase / metabolism; Glyceraldehyde-3-Phosphate Dehydrogenases / metabolism; Glycine Dehydrogenase (Decarboxylating); Glycosides / pharmacology; Magnesium / pharmacology; Nitrate Reductase (NADH); Nitrate Reductases / metabolism; Phosphorylation; Plants / enzymology; Plants / metabolism; Protein Binding / drug effects; Ribonucleosides / pharmacology; Tyrosine 3-Monooxygenase / metabolism
TL;DR: The factors that may regulate the binding of 14-3-3s to their target proteins are examined, and their possible roles in the regulation of the activity and proteolytic degradation of enzymes involved in primary carbon and nitrogen metabolism are discussed. (via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2001 journal article

Identification of a novel phosphorylation motif for CDPKs: Phosphorylation of synthetic peptides lacking basic residues at P-3/P-4

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 393(1), 61–66.

By: J. Huang *, S. Hardin n & S. Huber n

author keywords: calcium-dependent protein kinase; phosphorylation motif; synthetic peptide phosphorylation; Ca2+-ATPase; aquaporin
MeSH headings : Amino Acid Motifs; Amino Acid Sequence; Aquaporins / chemistry; Aquaporins / genetics; Aquaporins / metabolism; Binding Sites; Calcium-Transporting ATPases / chemistry; Calcium-Transporting ATPases / genetics; Calcium-Transporting ATPases / metabolism; Molecular Sequence Data; Peptides / chemistry; Peptides / genetics; Peptides / metabolism; Phosphorylation; Plant Proteins; Protein Kinases / metabolism; Sequence Homology, Amino Acid; Serine / chemistry; Spinacia oleracea / enzymology; Spinacia oleracea / genetics; Substrate Specificity
TL;DR: It is demonstrated that phosphorylation of the Ser(45)-ACA2 peptide is dependent on the cluster of basic residues found N-terminal (P-6 to P-9) as well as C-terminals (P + 1/P + 2) to the phosphorylated Ser. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2001 journal article

Phosphorylation of synthetic peptides by a CDPK and plant SNF1-related protein kinase. Influence of proline and basic amino acid residues at selected positions

PLANT AND CELL PHYSIOLOGY, 42(10), 1079–1087.

By: J. Huang * & S. Huber n

author keywords: CDPKs; phosphorylation motif; protein kinase specificity; SNF1-related protein kinase; Spinacia oleracea
MeSH headings : Amino Acid Sequence; Calcium-Calmodulin-Dependent Protein Kinases / chemistry; Calcium-Calmodulin-Dependent Protein Kinases / metabolism; Molecular Sequence Data; Peptides / chemistry; Peptides / metabolism; Phosphorylation; Plant Leaves / enzymology; Protein Serine-Threonine Kinases / chemistry; Protein Serine-Threonine Kinases / metabolism; Sequence Homology, Amino Acid; Spinacia oleracea / enzymology; Substrate Specificity
TL;DR: It is shown that a Pro at P-4 selectively inhibits phosphorylation of the peptide by a CDPK relative to a SnRK1, and proteins that may be selectively phosphorylated by SnRk1s, such as SPS in dicot species, or may beosphorylated in a pH-dependent manner. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2001 article

Post-translational regulation of nitrate reductase: mechanism, physiological relevance and environmental triggers

Kaiser, W. M., & Huber, S. C. (2001, October). JOURNAL OF EXPERIMENTAL BOTANY, Vol. 52, pp. 1981–1989.

By: W. Kaiser* & S. Huber n

author keywords: nitrate reductase; nitrite; nitric oxide; regulation; protein phosphorylation; 14-3-3-binding; signalling; sugars
MeSH headings : 14-3-3 Proteins; Adaptation, Physiological; Environment; Models, Biological; Nitrate Reductases / genetics; Nitrate Reductases / metabolism; Nitric Oxide / metabolism; Phosphorylation; Plants / metabolism; Protein Processing, Post-Translational / physiology; Tyrosine 3-Monooxygenase / metabolism
TL;DR: It is shown that light and oxygen availability are the major external triggers for the rapid and reversible modulation of NR activity, and that sugars and/or sugar phosphates are the internal signals which regulate the protein kinase(s) and phosphatase. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2000 review

Carbon and nitrogen metabolism and reversible protein phosphorylation

[Review of ]. ADVANCES IN BOTANICAL RESEARCH INCORPORATING ADVANCES IN PLANT PATHOLOGY, VOL 32, 32, 435–458.

By: D. Toroser* & S. Huber*

TL;DR: This chapter focuses on the regulation of activity of several enzymes involved in carbon- and nitrogen-metabolisms that are phosphorylated by either calmodulin-like domain protein kinases (CDPKs) or sucrose nonfermenting-1 (SNF1)-related protein. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2000 journal article

Modulation of 14-3-3 protein interactions with target polypeptides by physical and metabolic effectors

Plant and Cell Physiology, 41(4), 523–533.

By: G. Athwal*, C. Lombardo*, J. Huber*, S. Masters*, H. Fu * & S. Huber*

MeSH headings : 14-3-3 Proteins; Adenosine Monophosphate / pharmacology; Arabidopsis; Biosensing Techniques; Cations, Divalent / pharmacology; Fungal Proteins; Gene Expression Regulation, Enzymologic; Ligands; Nitrate Reductase; Nitrate Reductases / metabolism; Peptide Fragments / metabolism; Phosphopeptides / metabolism; Plant Proteins; Protein Binding / drug effects; Proteins / genetics; Proteins / metabolism; Protons; Recombinant Proteins / metabolism; Saccharomyces cerevisiae; Spinacia oleracea; Tyrosine 3-Monooxygenase
TL;DR: The proteins commonly referred to as 14-3-3s have recently come to prominence in the study of protein:protein interactions, having been shown to act as allosteric or steric regulators and possibly scaffolds, and to interact directly with cations, which bind and affect surface hydrophobicity. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: NC State University Libraries
Added: August 6, 2018

2000 journal article

Recombinant brassinosteroid insensitive 1 receptor-like kinase autophosphorylates on serine and threonine residues and phosphorylates a conserved peptide motif in vitro

PLANT PHYSIOLOGY, 124(2), 751–765.

By: M. Oh*, W. Ray, S. Huber n, J. Asara *, D. Gage* & S. Clouse*

MeSH headings : Amino Acid Motifs; Amino Acid Sequence; Arabidopsis / enzymology; Arabidopsis / genetics; Arabidopsis Proteins; Molecular Sequence Data; Phosphorylation; Phytosterols / metabolism; Protein Kinases / chemistry; Protein Kinases / genetics; Protein Kinases / metabolism; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Serine / chemistry; Signal Transduction; Threonine / chemistry
TL;DR: The inability of an active BRI1-KD to transphosphorylate an inactive mutant KD suggests that the mechanism of autophosphorylation is intramolecular, and it is interesting that recombinant BRI2-kD was also found to phosphorylate certain synthetic peptides in vitro. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2000 journal article

Regulation of a plant SNF1-related protein kinase by glucose-6-phosphate

PLANT PHYSIOLOGY, 123(1), 403–411.

By: D. Toroser n, Z. Plaut & S. Huber n

MeSH headings : Barium / metabolism; Glucose-6-Phosphate / pharmacology; Hydrogen-Ion Concentration; Magnesium / metabolism; Plants / enzymology; Protein Serine-Threonine Kinases / antagonists & inhibitors; Protein Serine-Threonine Kinases / isolation & purification; Protein Serine-Threonine Kinases / metabolism; Substrate Specificity
TL;DR: Glc-6-P inhibition of PK(III) activity potentially provides a mechanism for metabolic regulation of the reactions catalyzed by these important protein kinases. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2000 review

Regulation of sucrose metabolism in higher plants: Localization and regulation of activity of key enzymes

[Review of ]. CRITICAL REVIEWS IN PLANT SCIENCES, 19(1), 31–67.

By: H. Winter* & S. Huber

author keywords: actin cytoskeleton; 14-3-3 proteins; invertase; SNF1 protein kinase; CDPK; regulatory protein phosphorylation; sucrose-phosphate synthase; sucrose-sensing; sucrose synthase; translocation
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2000 review

Regulation of sucrose metabolism in higher plants: Localization and regulation of activity of key enzymes

[Review of ]. CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY, 35(4), 253–289.

By: H. Winter* & S. Huber n

Contributors: H. Winter* & S. Huber n

author keywords: actin cytoskeleton; 14-3-3 proteins; invertase; SNF1 protein kinase; CDPK; regulatory protein phosphorylation; sucrose-phosphate synthase; sucrose-sensing; sucrose synthase; translocation
MeSH headings : Actins / metabolism; Amino Acid Sequence; Cell Membrane / metabolism; Gene Expression Regulation, Plant; Glucosyltransferases / chemistry; Glucosyltransferases / genetics; Glucosyltransferases / metabolism; Glycoside Hydrolases / chemistry; Glycoside Hydrolases / metabolism; Molecular Sequence Data; Phosphorylation; Plant Leaves / enzymology; Plant Leaves / metabolism; Plant Proteins / metabolism; Plants / enzymology; Plants / metabolism; Sequence Alignment; Sucrose / metabolism; beta-Fructofuranosidase
TL;DR: The purpose of this review is to summarize some of the recent developments relating to regulation of activity and localization of key enzymes involved in sucrose metabolism in plants. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

1999 journal article

Nitrate reductase in higher plants: A case study for transduction of environmental stimuli into control of catalytic activity

Physiologia Plantarum, 105(2), 385–390.

By: W. Kaiser*, H. Weiner* & S. Huber n

TL;DR: In higher plants, cytosolic AMP and phosphate esters seem to regulate NR-PK and NR-PP activities, thereby adapting NR activity within minutes to the changing environment. (via Semantic Scholar)
Source: NC State University Libraries
Added: August 6, 2018

1999 journal article

Site-directed mutagenesis of serine 158 demonstrates its role in spinach leaf sucrose-phosphate synthase modulation

PLANT JOURNAL, 17(4), 407–413.

By: D. Toroser n, R. McMichael n, K. Krause, J. Kurreck *, U. Sonnewald *, M. Stitt *, S. Huber n

MeSH headings : Base Sequence; Circadian Rhythm; DNA Primers; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Glucosyltransferases / genetics; Glucosyltransferases / metabolism; Mutagenesis, Site-Directed; Plant Leaves / enzymology; Plants, Genetically Modified / genetics; Plants, Genetically Modified / physiology; Plants, Toxic; Serine / genetics; Serine / metabolism; Spinacia oleracea / enzymology; Spinacia oleracea / genetics; Tobacco / genetics; Tobacco / physiology
TL;DR: The results demonstrate the regulatory significance of Ser158 as the major site responsible for dark inactivation of spinach SPS in vivo, and indicate that the significance of phosphorylation is the introduction of a negative charge at the Ser158 position. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

1998 journal article

3-hydroxy-3-methylglutaryl-coenzyme a reductase kinase and sucrose-phosphate synthase kinase activities in cauliflower florets: Ca2+ dependence and substrate specificities

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 355(2), 291–300.

By: D. Toroser n & S. Huber n

author keywords: protein kinase; 3-hydroxy-3-methylglutaryl-CoA reductase; Brassica oleracea; sucrose-phosphate synthase; phosphorylation motif
MeSH headings : AMP-Activated Protein Kinases; Amino Acid Sequence; Binding Sites; Brassica; Calcium / physiology; Chromatography, Gel; Enzyme Activation; Glucosyltransferases / isolation & purification; Glucosyltransferases / metabolism; Hydroxymethylglutaryl CoA Reductases / metabolism; Molecular Sequence Data; Molecular Weight; Multienzyme Complexes / isolation & purification; Multienzyme Complexes / metabolism; Nitrate Reductase; Nitrate Reductase (NADH); Nitrate Reductases / metabolism; Peptides / chemical synthesis; Peptides / metabolism; Phosphorylation; Plant Structures / enzymology; Protein Kinases / isolation & purification; Protein Kinases / metabolism; Protein Serine-Threonine Kinases; Spinacia oleracea; Substrate Specificity
TL;DR: The novel aspects are that PKIII has not been detected in previous cauliflower studies, that SAMS* is a more specific peptide substrate to identify potential HMGR kinases, and that the major HM GR kinase in cauliflower is Ca2+ dependent. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

1998 journal article

Biological significance of divalent metal ion binding to 14-3-3 proteins in relationship to nitrate reductase inactivation

PLANT AND CELL PHYSIOLOGY, 39(10), 1065–1072.

By: G. Athwal n, J. Huber n & S. Huber n

author keywords: 14-3-3 protein; conformational change; fluorescence; metal binding site; nitrate reductase; protonation
MeSH headings : 14-3-3 Proteins; Binding Sites; Cations, Divalent; Metals / metabolism; Nitrate Reductase (NADH); Nitrate Reductases / antagonists & inhibitors; Nitrate Reductases / metabolism; Phosphopeptides / chemical synthesis; Phosphopeptides / metabolism; Protein Conformation; Proteins / chemistry; Proteins / metabolism; Protons; Tyrosine 3-Monooxygenase
TL;DR: It is proposed that protonation of amino acid residues brings about a similar effect to metal ion binding, and a possible regulatory mechanism, where the 14-3-3 protein has to be "primed" prior to binding a target protein, is discussed. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

1998 journal article

Identification of sucrose synthase as an actin-binding protein

FEBS LETTERS, 430(3), 205–208.

By: H. Winter n, J. Huber n & S. Huber n

Contributors: H. Winter n, J. L. Huber n & S. Huber n

author keywords: actin-binding protein; actin cytoskeleton; aldolase; sucrose synthase; phalloidin
MeSH headings : Actins / metabolism; Animals; Binding Sites; Detergents; Fructose-Bisphosphate Aldolase / metabolism; Glucosyltransferases / chemistry; Glucosyltransferases / isolation & purification; Glucosyltransferases / metabolism; Microfilament Proteins / chemistry; Microfilament Proteins / isolation & purification; Microfilament Proteins / metabolism; Molecular Weight; Octoxynol; Phosphorylation; Rabbits; Serum Albumin, Bovine; Solubility; Zea mays / enzymology
TL;DR: It is postulate that some of the soluble SuSy in the cytosol may be associated with the actin cytoskeleton in vivo, suggesting that a common or overlapping binding site may be involved. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: August 6, 2018

1998 journal article

Nitrate effect on carbon and nitrogen assimilating enzymes of maize hybrids representing seven eras of breeding

Maydica, 43(2), 83–94.

By: A. Purcino, C. Arellano, G. Athwal & S. Huber

Source: NC State University Libraries
Added: August 6, 2018

1998 journal article

Phosphorylated nitrate reductase and 14-3-3 proteins - Site of interaction, effects of ions, and evidence for an AMP-binding site on 14-3-3 proteins

PLANT PHYSIOLOGY, 118(3), 1041–1048.

By: G. Athwal*, J. Huber* & S. Huber n

MeSH headings : 14-3-3 Proteins; Adenosine Monophosphate / metabolism; Amino Acid Sequence; Binding Sites; Molecular Sequence Data; Nitrate Reductase; Nitrate Reductases / metabolism; Peptides / chemistry; Phosphorylation; Protein Binding; Proteins / metabolism; Sequence Homology, Amino Acid; Serine / metabolism; Spinacia oleracea / enzymology; Spinacia oleracea / metabolism; Tyrosine 3-Monooxygenase
TL;DR: It is shown that serine and threonine residues at the +6 to +8 positions, relative to the known regulatory binding site involving serine-543, are important in the interaction with GF14omega, a recombinant plant 14-3-3. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
6. Clean Water and Sanitation (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

1998 journal article

Site-specific regulatory interaction between spinach leaf sucrose-phosphate synthase and 14-3-3 proteins

FEBS LETTERS, 435(1), 110–114.

By: D. Toroser n, G. Athwal n & S. Huber n

author keywords: sucrose-phosphate synthase; 14-3-3 protein; Spinach; protein : protein interaction; surface plasmon resonance
MeSH headings : 14-3-3 Proteins; Amino Acid Sequence; Aminoimidazole Carboxamide / analogs & derivatives; Aminoimidazole Carboxamide / pharmacology; Binding Sites; Enzyme Activation / drug effects; Enzyme Inhibitors / metabolism; Enzyme Inhibitors / pharmacology; Glucosyltransferases / drug effects; Glucosyltransferases / metabolism; Magnesium / metabolism; Molecular Sequence Data; Peptide Fragments / metabolism; Phosphorylation; Plant Leaves / drug effects; Plant Leaves / enzymology; Proteins / metabolism; Proteins / pharmacology; Ribonucleotides / pharmacology; Serine / metabolism; Spinacia oleracea / drug effects; Spinacia oleracea / enzymology; Tyrosine 3-Monooxygenase
TL;DR: An Mg2+‐dependent interaction between spinach leaf sucrose‐phosphate synthase (SPS) and endogenous 14‐3‐3 proteins is reported, as evidenced by co‐elution during gel filtration and co‐immunoprecipitation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

1997 journal article

Correlation between apparent activation state of nitrate reductase (NR), NR hysteresis and degradation of NR protein

JOURNAL OF EXPERIMENTAL BOTANY, 48(312), 1367–1374.

By: W. Kaiser* & S. Huber n

author keywords: enzyme hysteresis; nitrate reductase; posttranslational modification; protein phosphorylation; protein turnover
TL;DR: The data indicate that NR protein phosphorylation not only controls the catalytic activity of NR, but also acts as a signal for NR protein degradation, with phospho-NR probably being a better substrate for protein degradation than the dephospho-form. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
6. Clean Water and Sanitation (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

1997 journal article

Light-regulated changes in abundance and polyribosome association of ferredoxin mRNA are dependent on photosynthesis

Plant Cell, 9(12), 2291–2300.

By: M. Petracek, L. Dickey*, S. Huber* & W. Thompson* 

Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

1997 journal article

Membrane association of sucrose synthase: changes during the graviresponse and possible control by protein phosphorylation

FEBS LETTERS, 420(2-3), 151–155.

By: H. Winter n, J. Huber n & S. Huber n

Contributors: H. Winter n, J. Huber n & S. Huber n

author keywords: protein phosphorylation; sucrose synthase; surface hydrophobicity; membrane association; gravity response; Zea mays L. pulvinus
MeSH headings : Adenosine Triphosphate / metabolism; Anilino Naphthalenesulfonates / metabolism; Blotting, Western; Cyclic AMP-Dependent Protein Kinases / antagonists & inhibitors; Cyclic AMP-Dependent Protein Kinases / metabolism; Electrophoresis, Polyacrylamide Gel; Enzyme Inhibitors / pharmacology; Fluorescent Dyes / metabolism; Glucosyltransferases / analysis; Glucosyltransferases / metabolism; Gravitation; Membrane Proteins / metabolism; Phosphorylation; Plant Proteins / metabolism; Precipitin Tests; Solubility; Uridine Diphosphate Glucose / metabolism; Zea mays / enzymology
TL;DR: It is postulate that phosphorylation of SuSy (and perhaps other membrane proteins) is involved in the release of the membrane‐bound enzyme in part as a result of decreased surface hydrophobicity. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018

1997 journal article

Protein phosphorylation as a mechanism for osmotic-stress activation of sucrose-phosphate synthase in spinach leaves

PLANT PHYSIOLOGY, 114(3), 947–955.

By: D. Toroser n & S. Huber n

MeSH headings : Amino Acid Sequence; Calcium / pharmacology; Chromatography, High Pressure Liquid; Chromatography, Ion Exchange; Conserved Sequence; Darkness; Enzyme Activation; Glucosyltransferases / biosynthesis; Glucosyltransferases / isolation & purification; Glucosyltransferases / metabolism; Light; Molecular Sequence Data; Osmolar Concentration; Peptide Fragments / chemistry; Peptide Fragments / metabolism; Phosphopeptides / chemistry; Phosphorylation; Phosphoserine; Plant Leaves; Protein Kinases / isolation & purification; Protein Kinases / metabolism; Sequence Alignment; Spinacia oleracea / enzymology; Spinacia oleracea / physiology
TL;DR: At least part of the osmotic stress activation of SPS in dark leaves results from phosphorylation of serine-424 catalyzed by a Ca2+-dependent, 150-kD protein kinase. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

1996 journal article

Phosphorylation of serine-15 of maize leaf sucrose synthase - Occurrence in vivo and possible regulatory significance

PLANT PHYSIOLOGY, 112(2), 793–802.

By: C. Huber n, J. Huber n, P. Liao n, D. Gage n, R. McMichael n, P. Chourey n, L. Hannah  n, K. Koch  n

MeSH headings : Amino Acid Sequence; Conserved Sequence; Gene Expression Regulation, Enzymologic; Glucosyltransferases / metabolism; Isoenzymes / metabolism; Molecular Sequence Data; Oligopeptides / metabolism; Peptide Mapping; Phosphopeptides / chemistry; Phosphorylation; Plant Leaves / enzymology; Plant Leaves / growth & development; Plant Leaves / metabolism; Plant Proteins; Protein Kinases / metabolism; Protein Serine-Threonine Kinases / isolation & purification; Sequence Analysis; Serine / metabolism; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Zea mays / enzymology; Zea mays / growth & development; Zea mays / metabolism
TL;DR: Conservation of the phosphorylated site, and the sequences surrounding it, among plant species suggests that phosphorylation of SuSy may be widespread, if not universal, in plants. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

1996 review

Post-translational regulation of nitrate reductase activity: A role for Ca2+ and 14-3-3 proteins

[Review of ]. TRENDS IN PLANT SCIENCE, 1(12), 432–438.

By: S. Huber n, M. Bachmann n & J. Huber n

TL;DR: These results suggest the convergence of several signal transduction pathways in the control of nitrate assimilation and implicate metabolites and 5′-AMP as possible effectors regulating the phosphorylation status of NR in vivo. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
6. Clean Water and Sanitation (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

1996 review

Role and regulation of sucrose-phosphate synthase in higher plants

[Review of ]. ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 47, 431–444.

By: S. Huber n & J. Huber n

author keywords: sucrose synthesis; spinach (Spinacia oleracea L); maize (Zea mays L); regulatory protein phosphorylation; protein kinase
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

1995 journal article

Metabolic activators of spinach leaf nitrate reductase: Effects on enzymatic activity and dephosphorylation by endogenous phosphatases

Planta, 196(1), 180.

By: S. Huber n & J. Huber n

TL;DR: Preliminary results suggest that light/dark transitions in vivo may alter the distribution of NR molecules between the low- and high-activity forms, and postulate that NR can exist in at least two states that differ in enzymatic activity. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: NC State University Libraries
Added: August 6, 2018

1994 journal article

Differential response of nitrate reductase and sucrose-phosphate synthase-activation to inorganic and organic salts, in vitro and in situ

Physiologia Plantarum, 92(2), 302.

By: S. Huber*, J. Huber & W. Kaiser

Source: NC State University Libraries
Added: August 6, 2018

1992 journal article

Apparent dependence of the light activation of nitrate reductase and sucrose-phosphate synthase activities in spinach leaves on protein synthesis

Plant and Cell Physiology, 33(5), 639.

By: S. Huber n, J. Huber n, W. Campbell* & M. Redinbaugh n

UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: NC State University Libraries
Added: August 6, 2018

1992 journal article

CARBON PARTITIONING AND GROWTH OF A STARCHLESS MUTANT OF NICOTIANA-SYLVESTRIS

PLANT PHYSIOLOGY, 99(4), 1449–1454.

By: S. Huber n & K. Hanson

TL;DR: The photosynthetic carbohydrate metabolism and growth of a starchless mutant of Nicotiana sylvestris that is deficient in plastid phosphoglucomutase is characterized and restricted growth of the mutant on short days is postulated, including slightly lower net photosynthesis and inability to synthesize starch in both source and sink tissues for use at night. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

1992 journal article

COMPARATIVE-STUDIES OF THE LIGHT-MODULATION OF NITRATE REDUCTASE AND SUCROSE-PHOSPHATE SYNTHASE ACTIVITIES IN SPINACH LEAVES

PLANT PHYSIOLOGY, 100(2), 706–712.

By: S. Huber*, J. Huber, W. Campbell & M. Redinbaugh

TL;DR: Overall, NR and SPS share many common features of control but are not identical in terms of regulation in situ, whereas with NR, the covalent modification is super-imposed on changes in the level of NR protein. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
Source: Web Of Science
Added: August 6, 2018

1992 journal article

ROLE OF SUCROSE-PHOSPHATE SYNTHASE IN SUCROSE METABOLISM IN LEAVES

PLANT PHYSIOLOGY, 99(4), 1275–1278.

By: S. Huber n & J. Huber

TL;DR: Results of preliminary studies with transgenic tomato plants expressing high levels of maize SPS support the postulate that SPS activity can influence the partitioning of carbon between starch and sucrose. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

1991 journal article

INVITRO PHOSPHORYLATION AND INACTIVATION OF SPINACH LEAF SUCROSE-PHOSPHATE SYNTHASE BY AN ENDOGENOUS PROTEIN-KINASE

BIOCHIMICA ET BIOPHYSICA ACTA, 1091(3), 393–400.

By: S. Huber n & J. Huber n

author keywords: PROTEIN PHOSPHORYLATION; ENZYME INACTIVATION; SUCROSE-PHOSPHATE SYNTHASE; PROTEIN KINASE; (SPINACH LEAF)
MeSH headings : Adenosine Triphosphate / metabolism; Glucosyltransferases / antagonists & inhibitors; Glucosyltransferases / isolation & purification; Glucosyltransferases / metabolism; Kinetics; Phosphorus Radioisotopes; Phosphorylation; Plants / enzymology; Protein Kinases / metabolism
TL;DR: The dependence of the rate of phosphorylation on SPS concentration suggested that SPS and the protein kinase are distinct enzymes, but have some tendency to associate especially in the presence of ethylene glycol. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

1991 journal article

REGULATION OF MAIZE LEAF SUCROSE-PHOSPHATE SYNTHASE BY PROTEIN-PHOSPHORYLATION

PLANT AND CELL PHYSIOLOGY, 32(3), 319–326.

By: S. Huber n & J. Huber n

author keywords: MAIZE; PROTEIN KINASE; PROTEIN PHOSPHORYLATION; SUCROSE BIOSYNTHESIS; SUCROSE-PHOSPHATE SYNTHASE
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

1991 journal article

SALT ACTIVATION OF SUCROSE-PHOSPHATE SYNTHASE FROM DARKENED LEAVES OF MAIZE AND OTHER C-4 PLANTS

PLANT AND CELL PHYSIOLOGY, 32(3), 327–333.

By: S. Huber n & J. Huber n

author keywords: MAIZE; PROTEIN CONFORMATION; SUCROSE BIOSYNTHESIS; SUCROSE-PHOSPHATE SYNTHASE, SALT ACTIVATION
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

1990 journal article

ACTIVATION OF SUCROSE-PHOSPHATE SYNTHASE FROM DARKENED SPINACH LEAVES BY AN ENDOGENOUS PROTEIN PHOSPHATASE

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 282(2), 421–426.

By: S. Huber n & J. Huber n

MeSH headings : Enzyme Activation; Ethers, Cyclic / pharmacology; Glucosyltransferases / metabolism; Hydrogen-Ion Concentration; Magnesium / pharmacology; Okadaic Acid; Phosphates / pharmacology; Phosphoprotein Phosphatases / analysis; Phosphoprotein Phosphatases / antagonists & inhibitors; Phosphoprotein Phosphatases / physiology; Phosphorylation; Photic Stimulation; Plants / enzymology
TL;DR: The results are consistent with the notion that SPS contains phosphorylation site(s) that reduce enzyme activation state and that dephosphorylation of these residue(S) is the mechanism of light activation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

1990 journal article

Regulation of spinach leaf sucrose-phosphate synthase by multisite phosphorylation

Current Topics in Plant Biochemistry and Physiology, 9, 329.

By: S. Huber & J. Huber

Source: NC State University Libraries
Added: August 6, 2018

1989 journal article

BIOCHEMICAL-MECHANISM FOR REGULATION OF SUCROSE ACCUMULATION IN LEAVES DURING PHOTOSYNTHESIS

PLANT PHYSIOLOGY, 91(2), 656–662.

By: S. Huber n

TL;DR: It is proposed that sucrose accumulation in vacuoles of species such as soybean and tobacco is prevented by acid invertase-mediated hydrolysis, and thus, the energy cost of sucrose production may be higher than is generally thought. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

1989 journal article

PHOTOSYNTHETIC DETERMINANTS OF GROWTH IN MAIZE PLANTS - EFFECTS OF NITROGEN NUTRITION ON GROWTH, CARBON FIXATION AND PHOTOCHEMICAL FEATURES

PLANT AND CELL PHYSIOLOGY, 30(8), 1063–1072.

By: S. Huber n, T. Sugiyama* & R. Alberte*

TL;DR: Objectives were to determine whether biomass production was related to carbon exchange rate and/or export rate and whether carbon exchange rates were related to photosynthetic enzyme levels, triose phosphate utilization and/ or photochemical capacity as expressed in the functional organization of the photosynthesis unit. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Source: Web Of Science
Added: August 6, 2018

1985 chapter

Role of potassium in photosynthesis and respiration

In Potassium in Agriculture (p. 369).

By: S. Huber n

UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: NC State University Libraries
Added: August 6, 2018

1984 article

EVIDENCE FOR CONTROL OF CARBON PARTITIONING BY FRUCTOSE 2,6-BISPHOSPHATE IN SPINACH LEAVES

PLANT PHYSIOLOGY, Vol. 74, pp. 445–447.

By: S. Huber n & D. Bickett n

UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

1983 journal article

ROLE OF SUCROSE-PHOSPHATE SYNTHASE IN PARTITIONING OF CARBON IN LEAVES

PLANT PHYSIOLOGY, 71(4), 818–821.

By: S. Huber*

TL;DR: The results suggest that a correlation exists between the activity of sucrose phosphate synthase and starch/sucrose levels in leaves. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

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