Works (7)
Updated: July 13th, 2023 21:15
2007 journal article
Characterization of a new family of protein kinases from Arabidopsis containing phosphoinositide 3/4-kinase and ubiquitin-like domains
BIOCHEMICAL JOURNAL, 409, 117–127.
author keywords: phosphoinositide kinase (PIK); phosphorylation; proteasome regulatory particle non-ATPase 10 subunit (proteasome RPN10 subunit); protein kinase; ubiquitin fusion degradation (UFD); ubiquitin-like domain (UBL domain)
MeSH headings : Adaptor Proteins, Signal Transducing / chemistry; Arabidopsis / metabolism; Arabidopsis Proteins / chemistry; Arabidopsis Proteins / metabolism; Catalysis; Class Ia Phosphatidylinositol 3-Kinase; Cloning, Molecular; DNA, Complementary / metabolism; Genetic Vectors; Humans; Phosphatidylinositol 3-Kinases / chemistry; Phosphatidylinositol 3-Kinases / metabolism; Phosphorylation; Phylogeny; Protein Kinases / chemistry; Protein Kinases / classification; Protein Structure, Tertiary; Recombinant Proteins / chemistry; Ubiquitin / chemistry; Ubiquitin / metabolism
TL;DR:
In vitro analysis of AtPI4Kgamma4 indicates that it interacts directly with, and phosphorylates, two proteins involved in the ubiquitin-proteasome system, namely UFD1 (ubiquitin fusion degradation 1) and RPN10 (regulatory particle non-ATPase 10).
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2007 journal article
Location and role of free cysteinyl residues in the Sindbis virus E1 and E2 glycoproteins
JOURNAL OF VIROLOGY, 81(12), 6231–6240.
MeSH headings : Amino Acid Sequence; Animals; Capsid / chemistry; Cell Line; Chromatography, Liquid; Cricetinae; Cysteine / chemistry; Disulfides / chemistry; Iodoacetamide / pharmacology; Mass Spectrometry; Membrane Glycoproteins / chemistry; Molecular Sequence Data; Mutation; Protein Conformation; Viral Envelope Proteins / chemistry
TL;DR:
Native virus particles alkylated with iodoacetamide demonstrated a 4-log decrease in viral infectivity, which suggests that the modification of free cysteinyl residues results in the loss of infectivity by destabilizing the virus particle or that a rearrangement of disulfide bonds, which is required forinfectivity, is blocked by the modification.
(via Semantic Scholar)
open access via jvi.asm.org (publisher PDF)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2007 journal article
Tandem mass spectrometry acquisition approaches to enhance identification of protein-protein interactions using low-energy collision-induced dissociative chemical crosslinking reagents
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 21(21), 3395–3408.
MeSH headings : Amino Acid Sequence; Bacillus subtilis / chemistry; Bacillus subtilis / metabolism; Bacterial Proteins / chemistry; Cross-Linking Reagents / chemistry; DNA-Binding Proteins / chemistry; Molecular Sequence Data; Peptide Mapping; Peptides / chemistry; Protein Conformation; Spectrometry, Mass, Electrospray Ionization; Tandem Mass Spectrometry / methods; Transcription Factors / chemistry
TL;DR:
The ability to segregate intrapeptide and interpeptide crosslinks using ITCID represents the first step towards high-throughput analysis of protein-protein cross links using the CID-CXL reagents.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
7. Affordable and Clean Energy
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2006 journal article
A mass spectrometry-based proteomic approach to study Marek's Disease Virus gene expression
Journal of Virological Methods, 135(1), 66–75.
author keywords: proteomics; gene expression; liquid chromatography; mass spectrometry; Marek's Disease Virus
MeSH headings : Animals; Cells, Cultured; Chick Embryo; Chromatography, Ion Exchange; Computational Biology; Databases, Protein; Fibroblasts / virology; Gas Chromatography-Mass Spectrometry; Gene Expression Profiling / methods; Herpesvirus 2, Gallid / genetics; Proteome / analysis; Proteome / isolation & purification; Proteomics / methods; Trypsin / metabolism; Viral Proteins / analysis; Viral Proteins / isolation & purification
TL;DR:
A mass spectrometry-based strategy was developed to identify viral proteins and to qualitatively examine their abundance in lytically infected chicken embryo fibroblast (CEF) cells and it is anticipated that this approach will be a viable method for determining how viral and host proteome changes occurring in Marek's Disease pathogenesis regulate the switch between the lytic and latent phases of the MDV life cycle.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, Crossref
Added: August 6, 2018
2006 journal article
Collision-induced dissociative chemical cross-linking reagents and methodology: Applications to protein structural characterization using tandem mass spectrometry analysis
ANALYTICAL CHEMISTRY, 78(23), 8059–8068.
MeSH headings : Amino Acid Sequence; Animals; Aspartic Acid / chemistry; Cross-Linking Reagents / chemistry; Glutathione Transferase / chemistry; Glutathione Transferase / genetics; Glutathione Transferase / metabolism; Models, Molecular; Molecular Sequence Data; Proline / chemistry; Protein Structure, Tertiary; Recombinant Fusion Proteins / chemistry; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / metabolism; Schistosoma japonicum / genetics; Schistosoma japonicum / metabolism; Serum Albumin, Bovine / chemistry; Serum Albumin, Bovine / genetics; Serum Albumin, Bovine / metabolism; Tandem Mass Spectrometry / methods
TL;DR:
Common acquisition approaches such as data-dependent acquisition experiments using ion trap mass spectrometers and product ion spectral analysis using SEQUEST were shown to be compatible with the CID-CXL-MS/MS reagents, obviating the requirement for high resolution and high mass accuracy measurements to identify both intra- and interpeptide cross-links.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2005 journal article
Identification and functional analysis of in vivo phosphorylation sites of the Arabidopsis BRASSINOSTEROID-INSENSITIVE1 receptor kinase
PLANT CELL, 17(6), 1685–1703.
MeSH headings : Arabidopsis / enzymology; Arabidopsis / genetics; Arabidopsis Proteins / chemistry; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Binding Sites / physiology; Cell Membrane / metabolism; Conserved Sequence / genetics; Mutation / genetics; Phosphorylation; Protein Kinases / chemistry; Protein Kinases / genetics; Protein Kinases / metabolism; Protein Serine-Threonine Kinases / chemistry; Protein Serine-Threonine Kinases / genetics; Protein Serine-Threonine Kinases / metabolism; Protein Structure, Tertiary / physiology; Serine / chemistry; Serine / metabolism; Signal Transduction / physiology; Threonine / chemistry; Threonine / metabolism
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2005 journal article
Novel protein purification system utilizing an N-terminal fusion protein and a caspase-3 cleavable linker
Protein Expression and Purification, 47(1), 311–318.
author keywords: caspase-3; fusion protein; protein expression; proteolysis
MeSH headings : Amino Acid Sequence; Caspase 3 / metabolism; Caspase 3 / physiology; Glutathione Transferase / genetics; Histidine / genetics; Hydrolysis; Molecular Sequence Data; Peptide Fragments / chemistry; Peptide Fragments / genetics; Peptide Fragments / metabolism; Protein Structure, Tertiary / genetics; Proteins / genetics; Proteins / isolation & purification; Proteins / metabolism; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / isolation & purification; Recombinant Fusion Proteins / metabolism
TL;DR:
A glutathione S-transferase-fusion protein vector with a caspase-3 consensus cleavage sequence located between the N-terminal GST tag and a target protein is designed and it is shown that the enzyme efficiently cleaves the fusion protein without leaving excess amino acids on the target protein.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, Crossref
Added: August 6, 2018