2011 journal article

Starch Self-Processing in Transgenic Sweet Potato Roots Expressing a Hyperthermophilic alpha-Amylase

BIOTECHNOLOGY PROGRESS, 27(2), 351–359.

By: M. Santa-Maria n, C. Yencho n, C. Haigler n, W. Thompson n, R. Kelly n & B. Sosinski n

author keywords: hyperthermophilic enzymes; starch conversion; transgenic plants; sweet potato; biofuels
MeSH headings : Crops, Agricultural / genetics; Hot Temperature; Ipomoea batatas / genetics; Ipomoea batatas / metabolism; Plant Roots / metabolism; Plants, Genetically Modified / enzymology; Plants, Genetically Modified / metabolism; Southeastern United States; Starch / metabolism; Thermotoga maritima / enzymology; alpha-Amylases / genetics; alpha-Amylases / physiology
TL;DR: The results presented here demonstrate that engineering plants with hyperthermophilic glycoside hydrolases can facilitate cost effective starch conversion to fermentable sugars and the use of sweet potato as an alternative near‐term energy crop should be considered. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2009 journal article

Plant cell calcium-rich environment enhances thermostability of recombinantly produced α-amylase from the hyperthermophilic bacterium Thermotoga maritime

Biotechnology and Bioengineering, 104(5), 947–956.

By: M. Santa-Maria n, C. Chou n, G. Yencho n, C. Haigler n, W. Thompson n, R. Kelly n, B. Sosinski n

author keywords: hyperthermophilic enzymes; starch processing; transgenic plants; tobacco NT1 Cell cultures; biofuel
MeSH headings : Calcium / pharmacology; Coenzymes / pharmacology; Enzyme Stability; Escherichia coli / enzymology; Escherichia coli / genetics; Hot Temperature; Plants, Genetically Modified / enzymology; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Thermotoga maritima / enzymology; Thermotoga maritima / genetics; Tobacco / enzymology; Tobacco / genetics; alpha-Amylases / chemistry; alpha-Amylases / genetics; alpha-Amylases / metabolism
TL;DR: In a proof of concept study, tobacco cell cultures were used as model system to test in planta production of a hyperthermophilic α‐amylase from Thermotoga maritima, demonstrating the importance of a calcium‐rich environment for thermostability, as well as the advantage of producing this enzyme directly in plant cells where calcium is readily available. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2009 journal article

Rapid shoot regeneration in industrial 'high starch' sweetpotato (Ipomoea batatas L.) genotypes

PLANT CELL TISSUE AND ORGAN CULTURE, 97(1), 109–117.

By: M. Santa-Maria n, K. Pecota n, C. Yencho n, G. Allen n & B. Sosinski n

author keywords: Meristem culture; High dry matter; Organogenesis; Storage roots; Sweet potato; Virus indexing
TL;DR: The results led to the establishment of optimized in vitro regeneration procedures for the novel high-starch sweetpotato (SP) genotypes ‘DM01-158’, ‘FTA94’ , ‘FT489’ and ‘PDM P4’ that are rapid and reliable. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

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