Rajendra Uprety

Works (11)

Updated: July 15th, 2023 21:19

2016 journal article

Genetically encoded optical activation of DNA recombination in human cells

CHEMICAL COMMUNICATIONS, 52(55), 8529–8532.

By: J. Luo *, E. Arbely *, J. Zhang n, C. Chou  n, R. Uprety n, J. Chin *, A. Deiters *

MeSH headings : Amino Acyl-tRNA Synthetases / genetics; Amino Acyl-tRNA Synthetases / metabolism; Catalytic Domain; HEK293 Cells; Humans; Integrases / chemistry; Integrases / metabolism; Lysine / metabolism; Methanosarcina / enzymology; Models, Molecular; Optical Phenomena; RNA, Transfer / metabolism; Recombination, Genetic; Tyrosine / metabolism
TL;DR: Two precisely regulated, light-activated Cre recombinase enzymes were generated through the site-specific incorporation of two genetically encoded photocaged amino acids in human cells. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2015 journal article

Conditional Control of Alternative Splicing through Light-Triggered Splice-Switching Oligonucleotides

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 137(10), 3656–3662.

By: J. Hemphill n, Q. Liu n, R. Uprety n, S. Samanta *, M. Tsang *, R. Juliano *, A. Deiters n

MeSH headings : Alternative Splicing / radiation effects; Animals; HeLa Cells; Humans; Light; Oligonucleotides / chemistry; Oligonucleotides / genetics; Zebrafish
TL;DR: This work addressed site-specific installation of light-removable nucleobase-caging groups as well as photocleavable backbone linkers into synthetic SSOs that enables optochemical OFF → ON and ON → OFF switching of their activity and thus precise control of alternative splicing. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2014 journal article

Control of Protein Function through Optochemical Translocation

ACS SYNTHETIC BIOLOGY, 3(10), 731–736.

By: H. Engelke *, C. Chou n, R. Uprety n, P. Jess* & A. Deiters n

author keywords: protein control; optogenetics; nuclear import; photocontrolled transcription; photocontrolled TEV-cleavage
MeSH headings : Active Transport, Cell Nucleus / radiation effects; Amino Acid Sequence; Endopeptidases / genetics; Endopeptidases / metabolism; Endopeptidases / radiation effects; Forkhead Box Protein O3; Forkhead Transcription Factors / genetics; Forkhead Transcription Factors / metabolism; Forkhead Transcription Factors / radiation effects; HEK293 Cells; Humans; Light; Matrix Attachment Region Binding Proteins / genetics; Matrix Attachment Region Binding Proteins / metabolism; Matrix Attachment Region Binding Proteins / radiation effects; Molecular Sequence Data; Nuclear Localization Signals / genetics; Photochemical Processes; Protein Engineering / methods; Synthetic Biology
TL;DR: Since the light-activated NLS is genetically encoded and optically triggered, it will prove useful to address a variety of problems requiring spatial and temporal control of protein function, for example, in stem-cell, developmental, and cancer biology. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2014 journal article

Genetic Encoding of Caged Cysteine and Caged Homocysteine in Bacterial and Mammalian Cells

CHEMBIOCHEM, 15(12), 1793–1799.

By: R. Uprety n, J. Luo *, J. Liu *, Y. Naro*, S. Samanta * & A. Deiters n

author keywords: amino acids; caged compounds; cysteine; gene technology; homocysteine; protein modifications
MeSH headings : Cells, Cultured; Cysteine / chemical synthesis; Cysteine / chemistry; Cysteine / genetics; Escherichia coli / cytology; Escherichia coli / genetics; Genetic Code / genetics; HEK293 Cells; Homocysteine / chemical synthesis; Homocysteine / chemistry; Homocysteine / genetics; Humans; Luciferases, Renilla / chemistry; Luciferases, Renilla / metabolism; Models, Molecular; Molecular Structure
TL;DR: The genetic incorporation of caged cysteine and caged homocysteine into proteins in bacterial and mammalian cells is reported to provide a new tool for the optochemical control of protein function in mammalian cells and expand the set of genetically encoded unnatural amino acids. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2014 journal article

Genetically Encoded Optochemical Probes for Simultaneous Fluorescence Reporting and Light Activation of Protein Function with Two-Photon Excitation

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 136(44), 15551–15558.

By: J. Luo n, R. Uprety n, Y. Naro*, C. Chou n, D. Nguyen *, J. Chin *, A. Deiters *

MeSH headings : Chromatography, Liquid; Fluorescence; HEK293 Cells; Humans; Methanosarcina barkeri / chemistry; Molecular Probes; Photons; Proteins / chemistry; Proteins / physiology; Tandem Mass Spectrometry
TL;DR: The small size of coumarin, the site-specific incorporation, the application as both a light-activated caging group and as a fluorescent probe, and the broad range of excitation wavelengths are advantageous over other genetically encoded photocontrol systems and provide a precise and multifunctional tool for cellular biology. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Cellular Delivery and Photochemical Activation of Antisense Agents through a Nucleobase Caging Strategy

ACS CHEMICAL BIOLOGY, 8(10), 2272–2282.

By: J. Govan n, R. Uprety n, M. Thomas  n, H. Lusic n, M. Lively* & A. Deiters n

MeSH headings : Base Sequence; Gene Transfer Techniques; HeLa Cells; Humans; Light; Models, Biological; Molecular Structure; Oligonucleotides, Antisense / chemistry; Oligonucleotides, Antisense / genetics; Oligonucleotides, Antisense / metabolism; Polymerase Chain Reaction; tat Gene Products, Human Immunodeficiency Virus / chemistry; tat Gene Products, Human Immunodeficiency Virus / genetics
TL;DR: The conjugation of multiple HIV TAT peptides to a hairpin-protected antisenseAgent through a light-cleavable nucleobase caging group allows for the facile delivery of the antisense agent without a transfection reagent, and photochemical activation offers precise control over gene expression. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2012 journal article

Regulation of transcription through light-activation and light-deactivation of triplex-forming oligonucleotides in mammalian cells

ACS Chemical Biology, 7(7), 1247–1256.

By: J. Govan n, R. Uprety n, J. Hemphill n, M. Lively* & A. Deiters n

MeSH headings : DNA / chemistry; DNA / metabolism; HEK293 Cells; Humans; Nucleic Acid Conformation; Oligonucleotides / chemistry; Oligonucleotides / metabolism; Photic Stimulation / methods; Transcription, Genetic / physiology
TL;DR: These are the first examples of light-regulated TFOs and their application in the photochemical activation and deactivation of gene expression and hairpin loop structures were found to significantly increase the efficacy of phosphodiester DNA-based T FOs in tissue culture. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: NC State University Libraries
Added: August 6, 2018

2012 journal article

Spatiotemporal control of microRNA function using light-activated antagomirs

MOLECULAR BIOSYSTEMS, 8(11), 2987–2993.

By: C. Connelly n, R. Uprety n, J. Hemphill n & A. Deiters n

MeSH headings : Antagomirs; Apoptosis / drug effects; Apoptosis / radiation effects; Cell Differentiation / drug effects; Cell Differentiation / radiation effects; Cell Line, Tumor; Cell Proliferation / drug effects; Cell Proliferation / radiation effects; Humans; Light; MicroRNAs / antagonists & inhibitors; MicroRNAs / metabolism; MicroRNAs / radiation effects; Oligonucleotides / chemistry; Oligonucleotides / pharmacology; Oligonucleotides / radiation effects; Photochemistry; Ultraviolet Rays
TL;DR: The presented approach enables the precise regulation of miRNA function and miRNA networks with unprecedented spatial and temporal resolution using UV irradiation and can be extended to any miRNA of interest. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2010 journal article

Expanding the Genetic Code of Yeast for Incorporation of Diverse Unnatural Amino Acids via a Pyrrolysyl-tRNA Synthetase/tRNA Pair

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 132(42), 14819–14824.

By: S. Hancock n, R. Uprety n, A. Deiters n & J. Chin  n

MeSH headings : Amino Acids / metabolism; Amino Acyl-tRNA Synthetases / metabolism; Genetic Code; RNA, Fungal / metabolism; RNA, Transfer / metabolism; Saccharomyces cerevisiae / enzymology; Saccharomyces cerevisiae / genetics
TL;DR: The discovery of a simple system through which variant pyrrolysyl-tRNA synthetase/tRNACUAPyl pairs created in Escherichia coli can be used to expand the genetic code of Saccharomyces cerevisiae is reported. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2010 journal article

Genetically encoding an aliphatic diazirine for protein photocrosslinking

CHEMICAL SCIENCE, 2(3), 480–483.

By: C. Chou n, R. Uprety n, L. Davis*, J. Chin * & A. Deiters n

TL;DR: A novel aliphatic diazirine amino acid and its genetically encoded, site-specific incorporation into proteins in bacterial and mammalian cells are reported and efficient photocrosslinking of a test protein is demonstrated in vitro and in vivo. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2010 journal article

Improved Synthesis of the Two-Photon Caging Group 3-Nitro-2-Ethyldibenzofuran and Its Application to a Caged Thymidine Phosphoramidite

ORGANIC LETTERS, 12(5), 916–919.

By: H. Lusic n, R. Uprety n & A. Deiters n

MeSH headings : Benzofurans / chemical synthesis; Benzofurans / chemistry; DNA / chemical synthesis; DNA / chemistry; Organophosphorus Compounds / chemistry; Photons; Thymidine Monophosphate / analogs & derivatives; Thymidine Monophosphate / chemistry
TL;DR: A new and efficient route to the recently reported 3-nitro-2-ethyldibenzofuran caging group was developed and its installation on a thymidine phosphoramidite is described. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

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