Jeane Marie Govan

Works (9)

Updated: August 19th, 2023 17:45

2015 journal article

Optically triggered immune response through photocaged oligonucleotides

TETRAHEDRON LETTERS, 56(23), 3639–3642.

By: J. Govan n, D. Young n, M. Lively* & A. Deiters n

author keywords: Oligonucleotide synthesis; Light-activation; Photocaged nucleotides; Immune response; Toll-like receptors
TL;DR: Synthetic photocaged CpGs via site-specific incorporation of nitropiperonyloxymethyl (NPOM)-caged thymidine residues enable the optical control of TLR9 function and thereby provide light-activation of an immune response. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Cellular Delivery and Photochemical Activation of Antisense Agents through a Nucleobase Caging Strategy

ACS CHEMICAL BIOLOGY, 8(10), 2272–2282.

By: J. Govan n, R. Uprety n, M. Thomas  n, H. Lusic n, M. Lively* & A. Deiters n

MeSH headings : Base Sequence; Gene Transfer Techniques; HeLa Cells; Humans; Light; Models, Biological; Molecular Structure; Oligonucleotides, Antisense / chemistry; Oligonucleotides, Antisense / genetics; Oligonucleotides, Antisense / metabolism; Polymerase Chain Reaction; tat Gene Products, Human Immunodeficiency Virus / chemistry; tat Gene Products, Human Immunodeficiency Virus / genetics
TL;DR: The conjugation of multiple HIV TAT peptides to a hairpin-protected antisenseAgent through a light-cleavable nucleobase caging group allows for the facile delivery of the antisense agent without a transfection reagent, and photochemical activation offers precise control over gene expression. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Optochemical control of RNA interference in mammalian cells

NUCLEIC ACIDS RESEARCH, 41(22), 10518–10528.

By: J. Govan n, D. Young  n, H. Lusic n, Q. Liu  n, M. Lively n & A. Deiters n

TL;DR: New photocaged guanosine and uridine RNA phosphoramidites are designed, synthesized and site-specifically incorporated into short RNA duplexes and demonstrated in the light-regulation of the expression of an exogenous green fluorescent protein reporter gene and an endogenous target gene, the mitosis motor protein, Eg5. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2012 journal article

Hydrogen Peroxide Induced Activation of Gene Expression in Mammalian Cells using Boronate Estrone Derivatives

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 51(36), 9066–9070.

By: J. Govan n, A. McIver n, C. Riggsbee n & A. Deiters n

author keywords: biosensors; estrogen receptor; estrone; gene expression; hydrogen peroxide
TL;DR: Keeping the boron out of the ER: A genetic switch was engineered that activates gene expression in the presence of H(2)O(2). (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2012 journal article

Regulation of transcription through light-activation and light-deactivation of triplex-forming oligonucleotides in mammalian cells

ACS Chemical Biology, 7(7), 1247–1256.

By: J. Govan n, R. Uprety n, J. Hemphill n, M. Lively* & A. Deiters n

MeSH headings : DNA / chemistry; DNA / metabolism; HEK293 Cells; Humans; Nucleic Acid Conformation; Oligonucleotides / chemistry; Oligonucleotides / metabolism; Photic Stimulation / methods; Transcription, Genetic / physiology
TL;DR: These are the first examples of light-regulated TFOs and their application in the photochemical activation and deactivation of gene expression and hairpin loop structures were found to significantly increase the efficacy of phosphodiester DNA-based T FOs in tissue culture. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: NC State University Libraries
Added: August 6, 2018

2011 journal article

Photochemical Control of DNA Decoy Function Enables Precise Regulation of Nuclear Factor kappa B Activity

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 133(33), 13176–13182.

By: J. Govan n, M. Lively* & A. Deiters n

MeSH headings : Alkaline Phosphatase / genetics; Gene Expression Regulation / radiation effects; Light; Methods; NF-kappa B / metabolism; Oligodeoxyribonucleotides / radiation effects; Photochemical Processes; Transcription, Genetic / drug effects
TL;DR: This is the first example of a caged DNA decoy for the photochemical regulation of gene expression in mammalian cells and represents an important addition to the toolbox of light-controlled gene regulatory agents. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Stabilization and Photochemical Regulation of Antisense Agents through PEGylation

BIOCONJUGATE CHEMISTRY, 22(10), 2136–2142.

By: J. Govan n, A. McIver n & A. Deiters n

MeSH headings : Animals; DNA, Antisense / chemical synthesis; DNA, Antisense / chemistry; DNA, Antisense / genetics; DNA, Antisense / metabolism; Gene Expression Regulation; HEK293 Cells; Humans; Luminescent Proteins / genetics; Mice; NIH 3T3 Cells; Nucleic Acid Denaturation; Photolysis; Polyethylene Glycols / chemistry; Ribonuclease H / metabolism; Ultraviolet Rays
TL;DR: By employing a photocleavable linker between the PEG group and the antisense agent, the paper was able to achieve light-induced deactivation of antisense activity and pave the way for precise regulation of gene expression in biological systems. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2010 journal article

Photocaged Morpholino Oligomers for the Light-Regulation of Gene Function in Zebrafish and Xenopus Embryos

Journal of the American Chemical Society, 132(44), 15644–15650.

By: A. Deiters n, R. Garner n, H. Lusic n, J. Govan n, M. Dush n, N. Nascone-Yoder n, J. Yoder n 

MeSH headings : Animals; Cells, Cultured; Embryo, Nonmammalian / metabolism; Gene Expression; Morpholines / chemistry; Oligonucleotides / genetics; Oligonucleotides / metabolism; Ultraviolet Rays; Xenopus laevis / genetics; Xenopus laevis / metabolism; Zebrafish / genetics; Zebrafish / metabolism
TL;DR: A novel and efficient synthetic route for incorporating photocaged monomeric building blocks directly into morpholino oligomers is reported and the utility of these caged morpholinos in the light-activated control of gene function in both cell culture and living embryos is demonstrated. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: August 6, 2018

2009 journal article

Photochemical Regulation of Restriction Endonuclease Activity

CHEMBIOCHEM, 10(10), 1612–1616.

By: D. Young n, J. Govan n, M. Lively* & A. Deiters n

author keywords: cage compounds; DNA cleavage; DNA enzymes; photochemistry
MeSH headings : Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Base Sequence; Benzodioxoles / chemistry; DNA / chemistry; DNA / radiation effects; DNA Cleavage; DNA Restriction Enzymes / genetics; DNA Restriction Enzymes / metabolism; Deoxyribonuclease BamHI / genetics; Deoxyribonuclease BamHI / metabolism; Deoxyribonuclease EcoRI / genetics; Deoxyribonuclease EcoRI / metabolism; Deoxyribonucleases, Type II Site-Specific / genetics; Deoxyribonucleases, Type II Site-Specific / metabolism; Substrate Specificity; Thymidine / analogs & derivatives; Thymidine / chemistry; Ultraviolet Rays
TL;DR: Photolabile protecting groups have been installed on DNA substrates and have been demonstrated to inhibit restriction endonuclease activity until removed by UV light irradiation, but these groups do not appear to dramatically affect initial binding of the enzyme to the DNA substrate, but rather prevent recognition of the specific cleavage site. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

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