Andrew James Loder

Works (9)

Updated: August 9th, 2023 15:05

2019 journal article

Determinants of sulphur chemolithoautotrophy in the extremely thermoacidophilic Sulfolobales

ENVIRONMENTAL MICROBIOLOGY, 21(10), 3696–3710.

By: B. Zeldes n, A. Loder n, J. Counts n, M. Haque n, K. Widney  n, L. Keller  n, S. Albers *, R. Kelly n 

MeSH headings : Autotrophic Processes; Chemoautotrophic Growth; Oxidation-Reduction; Oxidoreductases / metabolism; Sulfolobales / metabolism; Sulfur / metabolism; Thiosulfates / metabolism
TL;DR: Comparative transcriptomic analyses of Acidianus brierleyi, a chemolithoautotrophic sulfur oxidizer, and S. acidocaldarius DSM639 showed that while both share a strong transcriptional response to elemental sulfur, both failed to up-regulate key 3-HP/4-HB cycle genes used by A. briersleyi to drive chemolithOautotrophy. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 12, 2019

2017 journal article

Impact of growth mode, phase, and rate on the metabolic state of the extremely thermophilic archaeon Pyrococcus furiosus

BIOTECHNOLOGY AND BIOENGINEERING, 114(12), 2947–2954.

By: P. Khatibi n, C. Chou n, A. Loder n, J. Zurawski n, M. Adams * & R. Kelly n 

author keywords: continuous culture; growth phase; growth rate; hyperthermophiles; Pyrococcus furiosus; transcriptome
MeSH headings : Archaeal Proteins / metabolism; Batch Cell Culture Techniques / methods; Cell Proliferation / physiology; Energy Metabolism / physiology; Pyrococcus furiosus / growth & development; Pyrococcus furiosus / metabolism; Transcriptome / physiology
TL;DR: The results here suggest that cellular processes involved in carbon and electron flux in P. furiosus were significantly impacted by growth mode, phase and rate, factors that need to be taken into account when developing successful metabolic engineering strategies. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2016 journal article

Ancillary contributions of heterologous biotin protein ligase and carbonic anhydrase for CO2 incorporation into 3-hydroxypropionate by metabolically engineered Pyrococcus furiosus

Biotechnology and Bioengineering, 113(12), 2652–2660.

By: H. Lian, B. Zeldes, G. Lipscomb, A. Hawkins, Y. Han, A. Loder, D. Nishiyama, M. Adams, R. Kelly

Source: NC State University Libraries
Added: August 6, 2018

2016 journal article

Reaction kinetic analysis of the 3-hydroxypropionate/4-hydroxybutyrate CO2 fixation cycle in extremely thermoacidophilic archaea

METABOLIC ENGINEERING, 38, 446–463.

By: A. Loder n, Y. Han n, A. Hawkins n, H. Lian n, G. Lipscomb*, G. Schut *, M. Keller *, M. Adams *, R. Kelly n 

author keywords: CO2 fixation; 3-hydroxypropionate; 4-hydroxybutyrate; Metallosphaera sedula
MeSH headings : Archaea / metabolism; Carbon Dioxide / metabolism; Extremophiles / metabolism; Hydroxybutyrates / metabolism; Kinetics; Lactic Acid / analogs & derivatives; Lactic Acid / metabolism; Metabolic Clearance Rate; Metabolic Flux Analysis / methods; Metabolic Networks and Pathways / physiology; Models, Biological; Signal Transduction / physiology; Sulfolobaceae / metabolism
TL;DR: A reaction kinetics model was developed to examine the biological and biotechnological attributes of the 3HP/4HB cycle as it operates in Metallosphaera sedula and assess metabolic engineering strategies for incorporating CO2 into chemical intermediates and products of bioteschnological importance: acetyl-CoA, succinate, and 3-hydroxypropionate. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 journal article

Alcohol Selectivity in a Synthetic Thermophilic n-Butanol Pathway Is Driven by Biocatalytic and Thermostability Characteristics of Constituent Enzymes

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 81(20), 7187–7200.

By: A. Loder n, B. Zeldes n, G. Garrison n, G. Lipscomb*, M. Adams * & R. Kelly n 

MeSH headings : 1-Butanol / metabolism; Acetyl-CoA C-Acyltransferase / metabolism; Acyl Coenzyme A / metabolism; Alcohol Dehydrogenase / metabolism; Alcohol Oxidoreductases / metabolism; Aldehyde Oxidoreductases / metabolism; Biocatalysis; Chromatography, Gas; Clostridium acetobutylicum / metabolism; Clostridium thermocellum / metabolism; Thermoanaerobacter / metabolism
TL;DR: More-selective thermophilic versions of Bad, Bdh, and AdhE are needed to fully exploit biocatalytic n-butanol production at elevated temperatures. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 journal article

Bioprocessing analysis of Pyrococcus furiosus strains engineered for CO2-based 3-hydroxypropionate production

BIOTECHNOLOGY AND BIOENGINEERING, 112(8), 1533–1543.

By: A. Hawkins n, H. Lian n, B. Zeldes n, A. Loder n, G. Lipscomb*, G. Schut *, M. Keller *, M. Adams *, R. Kelly n 

author keywords: 3-hydroxypropionate; CO2 fixation; Metallosphaera sedula; Pyrococcus furiosus
MeSH headings : Bioreactors / microbiology; Carbon Dioxide / metabolism; Gene Expression Profiling; Hot Temperature; Lactic Acid / analogs & derivatives; Lactic Acid / metabolism; Maltose / metabolism; Metabolic Engineering / methods; Metabolic Networks and Pathways / genetics; Pyrococcus furiosus / genetics; Pyrococcus furiosus / metabolism; Pyrococcus furiosus / radiation effects; Sulfolobaceae / genetics
TL;DR: The results here support the prospects of using thermally diverse sources of pathways and enzymes in metabolically engineered strains designed for product formation at sub‐optimal growth temperatures. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 review

Extremely thermophilic microorganisms as metabolic engineering platforms for production of fuels and industrial chemicals

[Review of ]. FRONTIERS IN MICROBIOLOGY, 6.

By: B. Zeldes n, M. Keller *, A. Loder n, C. Straub n, M. Adams * & R. Kelly n 

author keywords: extreme thermophiles; metabolic engineering; bio-based chemicals; genetics; biotechnology
TL;DR: The issues and opportunities for extremely thermophilic metabolic engineering platforms are considered with an eye toward potential technological advantages for high temperature industrial biotechnology. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2014 journal article

A hybrid synthetic pathway for butanol production by a hyperthermophilic microbe

METABOLIC ENGINEERING, 27, 101–106.

By: M. Keller *, G. Lipscomb*, A. Loder n, G. Schut *, R. Kelly n  & M. Adams *

author keywords: Butanol; Biofuels; Hyperthermophile; Module; Hybrid; Pathway
MeSH headings : 1-Butanol / metabolism; Acetyl Coenzyme A / genetics; Acetyl Coenzyme A / metabolism; Metabolic Engineering / methods; Thermoanaerobacterium / genetics; Thermoanaerobacterium / metabolism
TL;DR: A synthetic metabolic pathway assembled from genes obtained from three different sources for conversion of acetyl-CoA to 1-butanol was demonstrated near 70°C in a microorganism that grows optimally near 100°C, and the module could also be used in thermophiles capable of degrading plant biomass. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2014 journal article

A mutant ('lab strain') of the hyperthermophilic archaeon Pyrococcus furiosus, lacking flagella, has unusual growth physiology

EXTREMOPHILES, 19(2), 269–281.

By: D. Lewis n, J. Notey n, S. Chandrayan *, A. Loder n, G. Lipscomb*, M. Adams *, R. Kelly n 

author keywords: Pyrococcus furiosus; Hyperthermophile; Archaeon; Growth physiology; Flagella
TL;DR: It is indicated that flagella play a key role, beyond motility, in the growth physiology of P. furiosus and, unlike the WT, showed minimal evidence of exopolysaccharide-based cell aggregation in post-exponential phase. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

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