Works (97)
Updated: August 29th, 2023 11:25
2021 journal article
Hyperglycemia minimally alters primary self-renewing human colonic epithelial cells while TNF alpha-promotes severe intestinal epithelial dysfunction
INTEGRATIVE BIOLOGY, 13(6), 139–152.
author keywords: hyperglycemia; glucose; TNF alpha; colon; large intestine; intestinal epithelium; primary tissue
MeSH headings : Colon; Epithelial Cells; Humans; Hyperglycemia; Intestinal Mucosa; Tumor Necrosis Factor-alpha
TL;DR:
Results suggest that TNFα acted as the dominant modulator of the epithelium relative to glucose, and that control of inflammation rather than glucose may be key to maintaining intestinal homeostasis.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: July 26, 2021
2021 journal article
Magnetically-propelled fecal surrogates for modeling the impact of solid-induced shear forces on primary colonic epithelial cells
BIOMATERIALS, 276.
author keywords: Colon; Intestine; Primary cells; Shear force; Magnetic; Hydrogel
MeSH headings : Colon; Epithelial Cells; Feces; Humans; Intestinal Mucosa; Muscle Contraction
open access via doi.org (repository)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
14. Life Below Water
(OpenAlex)
Source: Web Of Science
Added: September 7, 2021
2021 article
Stem/Proliferative and Differentiated Cells within Primary Murine Colonic Epithelium Display Distinct Intracellular Free Ca2+ Signal Codes
Mestril, S., Kim, R., Hinman, S. S., Gomez, S. M., & Allbritton, N. L. (2021, September 12). ADVANCED HEALTHCARE MATERIALS.
author keywords: Ca2+; colon; hydrogels; intestine; primary cells; stiffness
MeSH headings : Animals; Cell Differentiation; Colon; Epithelium; Intestinal Mucosa; Mice; Signal Transduction
TL;DR:
The ability to monitor Ca2+ oscillations with the spatial and temporal resolution offered by this platform, combined with its amenability to high‐content screens, provides a powerful tool for investigating real‐time communication within a wide range of primary tissues in addition to the colonic epithelium.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: September 20, 2021
2020 journal article
Automated platform for cell selection and separation based on four-dimensional motility and matrix degradation
ANALYST, 145(7), 2731–2742.
MeSH headings : Automation; Cell Line, Tumor; Cell Movement; Cell Separation / methods; Collagen / chemistry; Humans; Image Processing, Computer-Assisted; Microscopy, Confocal; Tissue Array Analysis
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: May 8, 2020
2020 review
Image-Based Live Cell Sorting
[Review of ]. TRENDS IN BIOTECHNOLOGY, 39(6), 613–623.
MeSH headings : Cell Physiological Phenomena; Cell Separation / instrumentation; Cell Separation / methods; Microfluidics
TL;DR:
The advent of IBCS marks a new paradigm in which cell phenotype and behavior can be explored with high resolution and tied to cellular physiological and omics data, providing a deeper understanding of single-cell physiology and the creation of cell lines with unique properties.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: June 10, 2021
2020 journal article
Microraft array-based platform for sorting of viable microcolonies based on cell-lethal immunoassay of intracellular proteins in microcolony biopsies
ANALYST, 145(7), 2649–2660.
MeSH headings : Animals; Cell Line, Tumor; Humans; Image Processing, Computer-Assisted; Immunoassay / instrumentation; Immunoassay / methods; Leukemia, Basophilic, Acute / metabolism; Leukemia, Basophilic, Acute / pathology; Magnetics; Microarray Analysis; Microscopy, Fluorescence; Phosphorylation; Rats; STAT3 Transcription Factor / metabolism
TL;DR:
A quad microraft array-based platform was developed to perform semi-automated cell sampling, bioassay, and banking on ultra-small sample sizes, and has the potential to enable colony screening and sorting based on assays not compatible with cell viability, greatly expanding the cell selection criteria available to identify cells with unique phenotypes for subsequent biomedical research.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: May 8, 2020
2020 journal article
Pooled CRISPR screens with imaging on microraft arrays reveals stress granule-regulatory factors
NATURE METHODS, 17(6), 636-+.
MeSH headings : CRISPR-Cas Systems / genetics; Clustered Regularly Interspaced Short Palindromic Repeats / genetics; Cytoplasm / metabolism; Humans; Machine Learning; Microscopy, Confocal / methods; Oxidative Stress / genetics; Protein Aggregates / genetics; RNA, Guide, Kinetoplastida / genetics; RNA-Binding Proteins / genetics; Tissue Array Analysis / methods
TL;DR:
A machine-learning model trained on nuclear morphology was developed and validated previously uncharacterized RBPs that modulate stress granule abundance, highlighting the applicability of the approach to facilitate image-based pooled CRISPR screens.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: May 26, 2020
2019 journal article
An in vitro intestinal platform with a self-sustaining oxygen gradient to study the human gut/microbiome interface
BIOFABRICATION, 12(1).
author keywords: intestine-on-chip; oxygen gradient; microbiome; intestinal crypt
MeSH headings : Bacteria / classification; Bacteria / growth & development; Bacteria / isolation & purification; Bacteria / metabolism; Cell Proliferation; Cells, Cultured; Colon / cytology; Colon / metabolism; Colon / microbiology; Epithelial Cells / cytology; Epithelial Cells / metabolism; Gastrointestinal Microbiome; Humans; In Vitro Techniques / methods; Intestines; Models, Biological; Oxygen / metabolism; Stem Cells / cytology; Stem Cells / metabolism
TL;DR:
This platform provides a simple system to create a physiological oxygen gradient across an intestinal mimic while simultaneously supporting anaerobe co-culture.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: December 16, 2019
2019 journal article
Assay and Isolation of Single Proliferating CD4+Lymphocytes Using an Automated Microraft Array Platform
IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING, 67(8), 2166–2175.
author keywords: Immune system; Media; Optical filters; Magnetic resonance imaging; Microscopy; Sorting; Cancer; Microarrays; lymphocyte; proliferation; cell sorting; immunology
MeSH headings : CD4-Positive T-Lymphocytes; Cell Separation; Cells, Cultured; Humans; Lymphocyte Activation
TL;DR:
A versatile platform for single cell analysis and isolation that can be applied in immunology research and clinical therapy development is developed, capable of both identification and isolation of proliferative T cells in an automated manner.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 10, 2020
2019 journal article
Automated sensing and splitting of stem cell colonies on microraft arrays
APL BIOENGINEERING, 3(3).
TL;DR:
A novel approach to label-free sensing and microcolony subsampling with the preservation of the mother colony holds the potential for hiPSC colony screening based on a wide range of properties including those measurable only by a cell destructive assay.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: October 21, 2019
2019 personal communication
Building a Thick Mucus Hydrogel Layer to Improve the Physiological Relevance of In Vitro Primary Colonic Epithelial Models
MeSH headings : Cell Culture Techniques / methods; Colon / metabolism; Humans; Hydrogels / metabolism; Intestinal Mucosa / metabolism; Mucus / metabolism; Mucus / physiology
TL;DR:
Strategies to generate a thick mucus layer are reported and its function act as a physiological barrier to bacteria and toxins are demonstrated.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: December 16, 2019
2019 journal article
Evaluation of human primary intestinal monolayers for drug metabolizing capabilities
JOURNAL OF BIOLOGICAL ENGINEERING, 13(1).
author keywords: Drug metabolism; Stiffness; Extracellular matrix; Small intestine
TL;DR:
The influence of mechanical properties of the culture substrate on protein expression and the activity of drug metabolizing enzymes as a critical factor in developing accurate assay protocols for pharmacokinetic studies using primary intestinal cells is highlighted.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: November 18, 2019
2019 journal article
Molecular transport through primary human small intestinal monolayers by culture on a collagen scaffold with a gradient of chemical cross-linking
JOURNAL OF BIOLOGICAL ENGINEERING, 13.
author keywords: Intestinal transport; Stiffness; Extracellular matrix; Small intestine
TL;DR:
The results of this comparison highlight the need to create in vitro intestinal transport platforms whose characteristics mimic the in vivo lamina propria in order to accurately recapitulate epithelial function.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: May 13, 2019
2019 journal article
Photopatterned Membranes and Chemical Gradients Enable Scalable Phenotypic Organization of Primary Human Colon Epithelial Models
ANALYTICAL CHEMISTRY, 91(23), 15240–15247.
MeSH headings : Cells, Cultured; Colon / cytology; Epithelial Cells / cytology; Humans; Microscopy, Fluorescence; Optical Imaging; Phenotype; Photochemical Processes; Polytetrafluoroethylene / chemistry
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: December 30, 2019
2019 review
Preserving single cells in space and time for analytical assays
[Review of ]. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 122.
author keywords: Laboratory medicine; Single-cell analysis; Sample preparation; Fixation; Preservation; Signaling; Analytical chemistry; Bioanalytical chemistry; Clinical assays
TL;DR:
Novel cell fixation techniques incorporate programmable fixation times, reversible bond formation and cleavage, chemoselective reactions, and improved analyte recovery to further the development of individualized, precision therapies for patients to yield improved clinical outcomes.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: February 10, 2020
2019 article
Selection and optimization of enzyme reporters for chemical cytometry
CHEMICAL AND SYNTHETIC BIOLOGY APPROACHES TO UNDERSTAND CELLULAR FUNCTIONS - PT B, Vol. 622, pp. 221–248.
MeSH headings : Animals; Enzyme Assays / methods; Humans; Kinetics; Peptide Hydrolases / metabolism; Peptide Library; Peptides / chemical synthesis; Peptides / chemistry; Peptides / metabolism; Single-Cell Analysis / methods; Solid-Phase Synthesis Techniques / methods; Substrate Specificity
TL;DR:
This method details the iterative approach used to design, characterize, and identify a peptidase-resistant peptide reporter which acts as a kinase substrate within intact cells.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: December 30, 2019
2018 journal article
"Fix and assay": separating in-cellulo sphingolipid reactions from analytical assay in time and space using an aldehyde-based fixative
ANALYST, 144(3), 961–971.
MeSH headings : Aldehydes / chemistry; Aldehydes / metabolism; Biological Assay / methods; Electrophoresis, Capillary / methods; Humans; K562 Cells; Single-Cell Analysis / methods; Sphingolipids / metabolism; Tissue Fixation / methods; U937 Cells
TL;DR:
A method utilizing aldehyde fixation to simultaneously terminate cellular reactions across a population, freezing reaction results in time prior to analytical analysis, enabling greater temporal control over intracellular reactions and improving future compatibility with clinical workflow is described.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: February 18, 2019
2018 journal article
A Monolayer of Primary Colonic Epithelium Generated on a Scaffold with a Gradient of Stiffness for Drug Transport Studies
ANALYTICAL CHEMISTRY, 90(22), 13331–13340.
MeSH headings : Animals; Atenolol / metabolism; Biological Transport / physiology; Caco-2 Cells; Chickens; Collagen / chemistry; Colon / physiology; Epithelium / physiology; Humans; Mice; Propranolol / metabolism; Rhodamine 123 / metabolism; Riboflavin / metabolism; Tissue Engineering / methods
TL;DR:
A collagen scaffold, in which the degree of collagen cross-linking was present as a gradient, was developed to support the proliferation of primary colonic cells, and it is anticipated that this platform of primary Colonic epithelium will find utility in drug development and physiological studies, since the tissue possesses high integrity and active transporters and metabolism similar to that in vivo.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: December 10, 2018
2018 journal article
Analysis of Interleukin 8 Secretion by a Stem-Cell-Derived Human-Intestinal-Epithelial-Monolayer Platform
ANALYTICAL CHEMISTRY, 90(19), 11523–11530.
MeSH headings : Cells, Cultured; Chemokine CCL2 / analysis; Enzyme-Linked Immunosorbent Assay; Humans; Interleukin-8 / analysis; Interleukin-8 / metabolism; Intestinal Mucosa / cytology; Intestinal Mucosa / drug effects; Intestinal Mucosa / metabolism; Porosity; Stem Cells / cytology; Tumor Necrosis Factor-alpha / pharmacology
TL;DR:
Results demonstrate that the described human-intestinal-monolayer platform has the potential for assays and screening of metabolites and compounds that alter the inflammatory response of the intestine.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: October 19, 2018
2018 review
Bioengineered systems and designer matrices that recapitulate the intestinal stem cell niche
[Review of ]. Cellular and Molecular Gastroenterology and Hepatology, 5(3), 440-.
Source: NC State University Libraries
Added: August 6, 2018
2018 journal article
Characterization of Tensioned PDMS Membranes for Imaging Cytometry on Microraft Arrays
ANALYTICAL CHEMISTRY, 90(7), 4792–4800.
MeSH headings : Automation; Cell Line, Tumor; Dimethylpolysiloxanes / chemistry; Flow Cytometry; Humans; Lung Neoplasms / diagnostic imaging; Microarray Analysis; Optical Imaging; Surface Properties
TL;DR:
The development of microfabrication strategies and physically motivated mathematical modeling of membrane deformation of PDMS microarrays are reported and this approach is readily applicable to control the deforming of similar membranes in MEMs devices, sensors, and microfluidics.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2018 journal article
LITE microscopy: Tilted light-sheet excitation of model organisms offers high resolution and low photobleaching
JOURNAL OF CELL BIOLOGY, 217(5), 1869–1882.
MeSH headings : Animals; Arabidopsis / cytology; Cell Line; Cell Nucleus / metabolism; Fluorescence; Fungi / cytology; Humans; Imaging, Three-Dimensional; Light; Microscopy, Fluorescence / methods; Models, Biological; Photobleaching; Reproducibility of Results; Time-Lapse Imaging
TL;DR:
A novel live-cell LSFM method, lateral interference tilted excitation (LITE), in which a tilted light sheet illuminates the detection objective focal plane without a sterically limiting illumination scheme, which is compatible with any detection objective, including oil immersion, without an upper NA limit.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2018 review
Primary Cell-Derived Intestinal Models: Recapitulating Physiology
[Review of ]. TRENDS IN BIOTECHNOLOGY, 37(7), 744–760.
MeSH headings : Cell Culture Techniques / methods; Cell Culture Techniques / trends; Cells, Cultured; Humans; Intestines / physiology; Models, Biological; Organoids / physiology; Tissue Engineering / methods; Tissue Engineering / trends
TL;DR:
This review highlights recent advances in the development and usage of primary cell-derived intestinal models incorporating monolayers, organoids, microengineered platforms, and macrostructured systems, and discusses the expected directions of the field.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: July 1, 2019
2017 journal article
A microengineered collagen scaffold for generating a polarized crypt-villus architecture of human small intestinal epithelium
BIOMATERIALS, 128, 44–55.
author keywords: Scaffold; Microfabrication; Intestine; Stem cell; Crypt; Villus
MeSH headings : Animals; Cell Compartmentation; Cell Differentiation / drug effects; Cell Movement / drug effects; Cell Proliferation / drug effects; Collagen / pharmacology; Cross-Linking Reagents / pharmacology; Humans; Hydrogel, Polyethylene Glycol Dimethacrylate / pharmacology; Intestinal Mucosa / drug effects; Intestinal Mucosa / physiology; Intestine, Small / drug effects; Intestine, Small / physiology; Mice; Organoids / drug effects; Porosity; Rats; Tissue Culture Techniques; Tissue Engineering / methods; Tissue Scaffolds / chemistry
TL;DR:
This new approach combining microengineered scaffolds, biophysical cues and chemical gradients to control the intestinal epithelium ex vivo can serve as a physiologically relevant mimic of the human small intestine, and is broadly applicable to model other tissues that rely on gradients for physiological function.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2017 journal article
Chemical fixation to arrest phospholipid signaling for chemical cytometry
JOURNAL OF CHROMATOGRAPHY A, 1523, 97–106.
author keywords: Capillary electrophoresis; Phospholipid; Phospholipase C; Diacylglycerol; Chemical cytometry; Chemical fixation
MeSH headings : Electrophoresis, Capillary; Signal Transduction; Tissue Fixation / methods; Type C Phospholipases / metabolism
TL;DR:
This methodology enables cell assay with the reporter to be separated in space and time from reporter metabolite quantification while preserving assay integrity, and demonstrates the capability of chemical fixation coupled to single-cell CZE to yield an accurate snapshot of cellular reactions with the probe.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2017 journal article
Formation of Human Colonic Crypt Array by Application of Chemical Gradients Across a Shaped Epithelial Monolayer
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY, 5(2), 113–130.
author keywords: Intestinal Epithelial Cells; Intestine-On-A-Chip; Stem Cell Niche; Polarized Crypt
TL;DR:
In vitro human colon crypt array accurately mimicked the architecture, luminal accessibility, tissue polarity, cell migration, and cellular responses of in vivo intestinal crypts.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2017 journal article
N-Gemini peptides: cytosolic protease resistance via N-terminal dimerization of unstructured peptides
CHEMICAL COMMUNICATIONS, 54(2), 204–207.
MeSH headings : Cell Line; Cytosol / enzymology; Dimerization; HeLa Cells; Humans; Molecular Structure; Peptide Hydrolases / metabolism; Peptides / chemical synthesis; Peptides / chemistry; Peptides / pharmacology; Protease Inhibitors / chemistry; Protease Inhibitors / pharmacology
TL;DR:
A synthetically simple strategy for increasing the lifetime of unstructured peptides in cytosolic environment via dimerization at the N-terminus to block threading into the catalytic cleft of cytosolics proteases is described.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2017 journal article
Nonsteroidal Anti-Inflammatory Drug -Induced Leaky Gut Modeled Using Polarized Monolayers of Primary Human Intestinal Epithelial Cells
ACS INFECTIOUS DISEASES, 4(1), 46–52.
author keywords: small intestine; leaky gut; bacterial translocation; NSAIDs; mitochondria; superoxide
MeSH headings : Anti-Inflammatory Agents, Non-Steroidal / adverse effects; Cell Membrane Permeability / drug effects; Epithelial Cells / drug effects; Epithelial Cells / metabolism; Humans; Intestinal Mucosa / drug effects; Intestinal Mucosa / metabolism; Mitochondria / drug effects; Mitochondria / metabolism
TL;DR:
The utility of this novel platform, representative of the human small intestinal epithelium, to understand NSAID toxicity, which can be applied to study multiple aspects of gut barrier function including defense against infectious pathogens and host-microbiota interactions, is established.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2017 journal article
Required hydrophobicity of fluorescent reporters for phosphatidylinositol family of lipid enzymes
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 409(29), 6781–6789.
author keywords: Fluorescent reporter; Chemical cytometry; Lipid enzyme; Phosphatidylinositol
MeSH headings : Enzymes / metabolism; Fluorescent Dyes / chemistry; Hydrophobic and Hydrophilic Interactions; Lipids / chemistry; Liposomes / metabolism; Phosphatidylinositols / metabolism
TL;DR:
For both enzymes, the fluorescent probe with a longer side chain functions as a better substrate than that with a shorter chain and works well in the presence of the endogenous lipid, highlighting the importance of hydrophobicity of side chains in fluorescent phosphoinositide reporters.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2017 journal article
Self-renewing monolayer of primary colonic or rectal epithelial cells
Cellular and Molecular Gastroenterology and Hepatology, 4(1), 165-.
Source: NC State University Libraries
Added: August 6, 2018
2016 journal article
An Integrated Chemical Cytometry Method: Shining a Light on Akt Activity in Single Cells
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 55(42), 13095–13098.
author keywords: biosensors; chemical cytometry; kinase activity probes; photochemistry; single-cell analysis
MeSH headings : Biosensing Techniques; Cell Line, Tumor; Flow Cytometry; Humans; Proto-Oncogene Proteins c-akt / metabolism; Single-Cell Analysis
TL;DR:
An integrated analysis system that utilizes a light-programmable, cell-permeable reporter deliverable simultaneously to many cells that enables programmable reaction times within the cell cytoplasm to evaluate oncogenic kinase activity in small clinical samples.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2016 journal article
Automated microraft platform to identify and collect non-adherent cells successfully gene-edited with CRISPR-Cas9
BIOSENSORS & BIOELECTRONICS, 91, 175–182.
author keywords: Microraft; Cell array; CRISPR-Cas9; Cytometry; Cell sorting
MeSH headings : Biosensing Techniques / instrumentation; Biosensing Techniques / methods; CRISPR-Cas Systems; Equipment Design; Genes, Reporter; Green Fluorescent Proteins / genetics; Humans; Image Processing, Computer-Assisted; K562 Cells; Leukemia / genetics; Mutation; Splicing Factor U2AF / genetics; Tissue Array Analysis / instrumentation; Tissue Array Analysis / methods; Transfection
TL;DR:
The automated system was used to examine the temporal variation in EGFP expression in cells transfected with CRISPR-Cas9 components for gene editing and successfully gene edited two cell colonies demonstrating the desired mutation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2016 journal article
Development of a protease-resistant reporter to quantify BCR-ABL activity in intact cells
ANALYST, 141(21), 6008–6017.
MeSH headings : Animals; Cell Line, Tumor; Fusion Proteins, bcr-abl / metabolism; Leukemia, Myelogenous, Chronic, BCR-ABL Positive; Mice; Peptide Hydrolases / metabolism; Peptides / metabolism; Phosphorylation; Protein-Tyrosine Kinases / metabolism; Substrate Specificity
TL;DR:
A peptidase-resistant ABL kinase substrate was developed by identifying protease-susceptible bonds on an ABL substrate peptide and replacing flanking amino acids with non-native amino acids, and the designed peptide X-A acts as a long-lived BCR-ABL kinase reporter in the leukemic cells possessing the BCR/ABL mutation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2016 journal article
Identification and isolation of antigen-specific cytotoxic T lymphocytes with an automated microraft sorting system
INTEGRATIVE BIOLOGY, 8(12), 1208–1220.
MeSH headings : Antigens, Viral / immunology; CD4-Positive T-Lymphocytes / immunology; CD8-Positive T-Lymphocytes / immunology; Cell Separation; Cells, Cultured; Epitope Mapping / instrumentation; Equipment Design; Equipment Failure Analysis; Humans; Immunoassay / instrumentation; Killer Cells, Natural / immunology; Lab-On-A-Chip Devices; T-Lymphocytes, Cytotoxic / immunology; Tissue Array Analysis / instrumentation
TL;DR:
A microraft array methodology is developed that automatically measures the ability of individual T cells to kill a population of target cells and viably sorts specific cells into a 96-well plate for expansion, enabling rapid identification of highly cytotoxic CD8+ T cells.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2016 journal article
In vitro polarization of colonoids to create an intestinal stem cell compartment
PLoS One, 11(4).
Source: NC State University Libraries
Added: August 6, 2018
2016 journal article
Single Cell Chemical Cytometry of Akt Activity in Rheumatoid Arthritis and Normal Fibroblast-like Synoviocytes in Response to Tumor Necrosis Factor alpha
ANALYTICAL CHEMISTRY, 88(15), 7786–7792.
MeSH headings : Arthritis, Rheumatoid / metabolism; Arthritis, Rheumatoid / pathology; Cells, Cultured; Chromones / pharmacology; Electrophoresis, Capillary; Fibroblasts / cytology; Humans; Insulin / pharmacology; Morpholines / pharmacology; Phosphorylation; Proto-Oncogene Proteins c-akt / antagonists & inhibitors; Proto-Oncogene Proteins c-akt / genetics; Proto-Oncogene Proteins c-akt / metabolism; RNA Interference; RNA, Small Interfering / metabolism; Receptors, Tumor Necrosis Factor, Type I / metabolism; Signal Transduction / drug effects; Single-Cell Analysis; Synoviocytes / cytology; Synoviocytes / drug effects; Synoviocytes / metabolism; Tumor Necrosis Factor-alpha / pharmacology
TL;DR:
It is expected that chemical cytometry studies pairing peptide reporters with capillary electrophoresis will provide valuable data regarding aberrant kinase activity from small samples of clinical interest, suggesting heterogeneity in Akt and protease activity that may play a role in the RA-affected joint.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2015 journal article
Analysis of sphingosine kinase activity in single natural killer cells from peripheral blood
INTEGRATIVE BIOLOGY, 7(4), 392–401.
MeSH headings : Cells, Cultured; Enzyme Activation; Humans; Killer Cells, Natural / enzymology; Phosphotransferases (Alcohol Group Acceptor) / blood; Phosphotransferases (Alcohol Group Acceptor) / chemistry
TL;DR:
In contrast to the SK activity, S1PP activity was homogeneous across the peripheral blood NK cells, suggesting a bias in the SK pathway towards proliferation and migration, activities supported by S1P.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2015 journal article
Array-Based Platform To Select, Release, and Capture Epstein-Barr Virus-Infected Cells Based on Intercellular Adhesion
ANALYTICAL CHEMISTRY, 87(24), 12281–12289.
MeSH headings : Cell Adhesion; Cell Separation / instrumentation; Cell Separation / methods; Cell Survival; Dimethylpolysiloxanes / chemistry; Epstein-Barr Virus Nuclear Antigens / genetics; Epstein-Barr Virus Nuclear Antigens / metabolism; Herpesvirus 4, Human / physiology; Humans; K562 Cells; Microarray Analysis / instrumentation; Nylons / chemistry; Particle Size; Single-Cell Analysis / instrumentation; Surface Properties; Tumor Cells, Cultured; Viral Proteins / genetics; Viral Proteins / metabolism
TL;DR:
Microraft arrays are a versatile method enabling separation of cells based on complicated and as yet poorly understood cell phenotypes, enabling release and collection of the cells without the need for cell attachment to the microraft surface.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2015 journal article
Co-fabrication of chitosan and epoxy photoresist to form microwell arrays with permeable hydrogel bottoms
BIOMATERIALS, 74, 77–88.
author keywords: Hydrogel; Microwell array; Chitosan; Microfabrication
MeSH headings : Animals; Cell Line; Cell Proliferation; Cell Survival; Chitosan / chemistry; Epoxy Compounds / chemistry; Hydrogels / chemistry; Mice; Microscopy, Electron, Scanning; Permeability
TL;DR:
This co-fabrication of a micropatterned epoxy photoresist film with a chitosan film to create a freestanding array of permeable, hydrogel-bottomed microwells will enable high-throughput studies of cellular heterogeneity with increased ability to customize dynamic interrogations compared to materials in currently available technologies.
(via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018
2015 journal article
Magnetic Alignment of Microelements Containing Cultured Neuronal Networks for High-Throughput Screening
JOURNAL OF BIOMOLECULAR SCREENING, 20(9), 1091–1100.
author keywords: high-throughput screening; primary neurons; magnetic centering; microfabrication; finite element analysis; microraft arrays
MeSH headings : Animals; Cell Survival; Cells, Cultured; Drug Evaluation, Preclinical; High-Throughput Screening Assays / instrumentation; High-Throughput Screening Assays / methods; Magnetic Phenomena; Nerve Net / cytology; Neurons / drug effects; Neurons / physiology; Rats, Sprague-Dawley
TL;DR:
A magnet array plate is developed, compatible with HTS fluid-handling systems, to center microrafts within wells, finding a centering efficiency of 100%, compared with 4.35% using a flat magnet, and concluding that micRORafts could be centered after settling randomly within the well, overcoming friction.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2015 journal article
Optimizing Wnt-3a and R-spondin1 concentrations for stem cell renewal and differentiation in intestinal organoids using a gradient-forming microdevice
RSC ADVANCES, 5(91), 74881–74891.
TL;DR:
The gradient culture system enabled efficient determination of the minimal growth factor concentrations needed to produce a physiologically-relevant colonoid phenotype, and lower factor concentrations yielded the added benefit of a more morphologically appropriate colonoid possessing columnar cells surrounding a central lumen with active crypt-like bud formation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2015 journal article
Pronase E-Based Generation of Fluorescent Peptide Fragments: Tracking Intracellular Peptide Fate in Single Cells
ANALYTICAL CHEMISTRY, 87(15), 7987–7995.
MeSH headings : Amino Acid Sequence; Fluorescence; Intracellular Space / metabolism; Models, Biological; Molecular Sequence Data; Peptide Fragments / biosynthesis; Peptide Fragments / metabolism; Pronase / metabolism; Proteolysis; Single-Cell Analysis / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
TL;DR:
A rapid and inexpensive methodology to generate fluorescent peptide fragments from a parent sequence with diverse chemical properties, including aliphatic, nonpolar, basic, acidic, and non-native amino acids is described.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2015 journal article
Rational Design of a Dephosphorylation-Resistant Reporter Enables Single-Cell Measurement of Tyrosine Kinase Activity
ACS CHEMICAL BIOLOGY, 11(2), 355–362.
MeSH headings : Cell Line, Tumor; Enzyme Assays / methods; ErbB Receptors / chemistry; ErbB Receptors / metabolism; Humans; Phosphorylation; Single-Cell Analysis / methods; Tetrahydroisoquinolines / chemistry; Tetrahydroisoquinolines / metabolism; Tyrosine / chemistry; Tyrosine / metabolism
TL;DR:
This work describes a peptidic reporter that, upon phosphorylation by the epidermal growth factor receptor (EGFR), is resistant to dephosphorylation both in vitro and in cellulo, and contains a conformationally constrained phosphorylatable moiety.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2015 journal article
Separation of peptide fragments of a protein kinase C substrate fused to a beta-hairpin by capillary electrophoresis
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 407(30), 8999–9008.
author keywords: Capillary electrophoresis; beta-Hairpin; Peptide separation; PKC; Protease resistance; Reporter
MeSH headings : Electrophoresis, Capillary / methods; Molecular Structure; Peptide Fragments / chemistry; Peptide Fragments / isolation & purification; Phosphorylation; Protein Kinase C / chemistry
TL;DR:
Capillary-electrophoretic separation conditions for WKpG β-hairpin peptide fused to a protein kinase C (PKC) substrate were developed and could be detected and separated from peptidase-generated fragments produced in a cell lysate.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2015 journal article
Single-cell functional analysis of parathyroid adenomas reveals distinct classes of calcium sensing behaviour in primary hyperparathyroidism
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 20(2), 351–359.
author keywords: hyperparathyroidism; parathyroid; adenoma; calcium; single-cell analysis
MeSH headings : Adenoma / metabolism; Calcium / metabolism; Calcium Signaling / physiology; Cell Line; Humans; Hyperparathyroidism, Primary / metabolism; Parathyroid Neoplasms / metabolism; Receptors, Calcium-Sensing / metabolism; Single-Cell Analysis / methods
TL;DR:
Calcium EC50 values from a series of parathyroid adenomas revealed that the tumours segregated into two distinct categories, indicating the existence of a previously unappreciated biochemical sub‐classification of PHPT tumours, possibly reflecting distinct etiological mechanisms.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2014 journal article
An Immature Retroviral RNA Genome Resembles a Kinetically Trapped Intermediate State
JOURNAL OF VIROLOGY, 88(11), 6061–6068.
MeSH headings : Acylation; DNA Primers / genetics; Dimerization; Electrophoresis, Capillary; Genome, Viral / genetics; Models, Molecular; Moloney murine leukemia virus / genetics; RNA, Viral / genetics; Virion / genetics; Virion / growth & development
TL;DR:
It is found that the genomic RNA is packaged in a high-energy state, suggesting that interactions within the virion hold or capture the RNA before it reaches its most stable state.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
7. Affordable and Clean Energy
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2014 journal article
Dynamics and evolution of beta-catenin-dependent Wnt signaling revealed through massively parallel clonogenic screening
INTEGRATIVE BIOLOGY, 6(7), 673–684.
MeSH headings : Cell Line, Tumor; Clone Cells; Humans; Image Processing, Computer-Assisted; Kinetics; Luminescent Proteins / chemistry; Luminescent Proteins / metabolism; Microscopy, Fluorescence / methods; Wnt Proteins / metabolism; Wnt Signaling Pathway; beta Catenin / metabolism
TL;DR:
A generational-dependent trend in Wnt signaling in A375 cells is revealed that provides insight into the pathway's mechanisms of positive feedback and self-inhibition, which is seen as a challenge to be overcome in the development of novel cell lines and reporter-based assays.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2014 journal article
Measurement of Protein Kinase B Activity in Single Primary Human Pancreatic Cancer Cells
ANALYTICAL CHEMISTRY, 86(9), 4573–4580.
MeSH headings : Adenocarcinoma / enzymology; Adenocarcinoma / pathology; Blotting, Western; Cell Line, Tumor; Humans; Pancreatic Neoplasms / enzymology; Pancreatic Neoplasms / pathology; Proto-Oncogene Proteins c-akt / metabolism
TL;DR:
The ability of chemical cytometry to identify important differences in enzymatic behavior between primary cells and tissue-cultured cell lines is demonstrated.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2014 journal article
Response of single leukemic cells to peptidase inhibitor therapy across time and dose using a microfluidic device
INTEGRATIVE BIOLOGY, 6(2), 164–174.
MeSH headings : Cell Survival / drug effects; Dose-Response Relationship, Drug; Glycine / administration & dosage; Glycine / analogs & derivatives; Glycine / pharmacology; Humans; Hydroxamic Acids / administration & dosage; Hydroxamic Acids / pharmacology; Leukemia, Myeloid, Acute / drug therapy; Leukemia, Myeloid, Acute / enzymology; Longitudinal Studies; Microscopy, Fluorescence; Peptide Hydrolases / metabolism; Protease Inhibitors / administration & dosage; Protease Inhibitors / pharmacology; Regression Analysis; U937 Cells
TL;DR:
The results suggest that additional enzymes may become inhibited at doses >1 μM and >2 μM, demonstrating the utility of single-cell data to yield novel biological hypotheses.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2014 journal article
Single-cell sphingosine kinase activity measurements in primary leukemia
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 406(27), 7027–7036.
author keywords: Single-cell analysis; Sphingosine kinase; Capillary electrophoresis; Leukemia; Primary cells
MeSH headings : Electrophoresis, Capillary; Humans; K562 Cells; Leukemia / enzymology; Phosphotransferases (Alcohol Group Acceptor) / metabolism
TL;DR:
The variability in SK signaling may have important implications for SK inhibitors as therapeutics for leukemia and demonstrates the value of single-cell analysis in characterizing the nature of oncogenic signaling in cancer.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2014 journal article
Small Sample Sorting of Primary Adherent Cells by Automated Micropallet Imaging and Release
CYTOMETRY PART A, 85A(7), 642–649.
author keywords: image cytometry; xenografts; cell sorting; micropallet arrays
MeSH headings : Animals; Antigens, Neoplasm / analysis; Cell Adhesion Molecules / analysis; Colorectal Neoplasms / pathology; Electronic Data Processing; Epithelial Cell Adhesion Molecule; Flow Cytometry / methods; Heterografts / cytology; Humans; Image Processing, Computer-Assisted; Mice; Mice, Nude; Pancreatic Neoplasms / pathology; Staining and Labeling; Tumor Cells, Cultured
TL;DR:
A platform for imaging cytometry integrated with micropallet array technology is developed to perform automated cell sorting on very small samples obtained from PDX models of pancreatic and colorectal cancer using antibody staining of EpCAM (CD326) as a selection criteria, demonstrating the ability to automate and efficiently separate samples with very low number of cells.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2013 journal article
A comparative analysis of the ubiquitination kinetics of multiple degrons to identify an ideal targeting sequence for a proteasome reporter
PLoS One, 8(10).
Source: NC State University Libraries
Added: August 6, 2018
2013 journal article
Array of biodegradable microrafts for isolation and implantation of living, adherent cells
RSC Advances, 3(24), 9264–9272.
Source: NC State University Libraries
Added: August 6, 2018
2013 journal article
Automated Capillary Electrophoresis System for Fast Single-Cell Analysis
ANALYTICAL CHEMISTRY, 85(9), 4797–4804.
MeSH headings : Animals; Automation; Electrophoresis, Capillary; PC12 Cells; Rats; Single-Cell Analysis; Tumor Cells, Cultured
TL;DR:
An automated platform employing microfabricated cell traps and a three-channel system for rapid buffer exchange for fast single-cell CE was capable of stable and reproducible separations of biological compounds in hundreds of adherent and nonadherent cells, enabling measurements of previously uncharacterized biological phenomena.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
15. Life on Land
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2013 journal article
Capture and 3D culture of colonic crypts and colonoids in a microarray platform
Lab on a Chip, 13(23), 4625–4634.
Source: NC State University Libraries
Added: August 6, 2018
2013 journal article
Characterization of freestanding photoresist films for biological and MEMS applications
Journal of Micromechanics and Microengineering, 23(2).
Source: NC State University Libraries
Added: August 6, 2018
2013 journal article
Ex Vivo Chemical Cytometric Analysis of Protein Tyrosine Phosphatase Activity in Single Human Airway Epithelial Cells
ANALYTICAL CHEMISTRY, 86(2), 1291–1297.
MeSH headings : Bronchi / cytology; Bronchi / drug effects; Bronchi / enzymology; Cell Line; Electrophoresis, Capillary; Enzyme Inhibitors / pharmacology; Epithelial Cells / cytology; Epithelial Cells / drug effects; Epithelial Cells / enzymology; Half-Life; Humans; Hydrolysis; Microinjections; Naphthoquinones / pharmacology; Phosphoproteins / administration & dosage; Phosphoproteins / metabolism; Primary Cell Culture; Protein Tyrosine Phosphatases / analysis; Protein Tyrosine Phosphatases / antagonists & inhibitors; Protein Tyrosine Phosphatases / metabolism; Single-Cell Analysis; Vanadates / pharmacology
TL;DR:
The utility and applicability of the microinjection of a fluorescent phosphopeptide that is hydrolyzed specifically by PTPs for ex vivo quantification of PTP activity in small, heterogeneous, human cells and tissues are demonstrated.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2013 journal article
Fabrication of 3D Microstructures from Interactions of Immiscible Liquids with a Structured Surface
ADVANCED MATERIALS, 25(30), 4107–4112.
MeSH headings : Colloids / chemistry; Dimethylpolysiloxanes / chemistry; Macromolecular Substances / chemistry; Materials Testing; Microfluidics / instrumentation; Microfluidics / methods; Molecular Conformation; Molecular Imprinting / methods; Solutions / chemistry; Surface Properties
TL;DR:
A new lithography technique is presented that exploits the interactions of immiscible liquids with a structured surface and easily produces curved and/or multi-level structures out of a variety of photoactive and non-photoactive materials.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2013 journal article
Isolation and in Vitro Culture of Rare Cancer Stem Cells from Patient-Derived Xenografts of Pancreatic Ductal Adenocarcinoma
ANALYTICAL CHEMISTRY, 85(15), 7271–7278.
MeSH headings : Animals; Carcinoma, Pancreatic Ductal / pathology; Cell Culture Techniques / methods; Cell Line, Tumor; Cell Separation / methods; Cell Survival; Cell Transformation, Neoplastic; Humans; Image Processing, Computer-Assisted; Mice; Neoplastic Stem Cells / pathology; Pancreatic Neoplasms / pathology
TL;DR:
A direct comparison of this technology with fluorescence-activated cell sorting (FACS) demonstrated that micropallet arrays offered enhanced post sorting purity (100), yield (100%), and viability (94-100%) for rare cell isolation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2013 journal article
Measurement of Protein Tyrosine Phosphatase Activity in Single Cells by Capillary Electrophoresis
ANALYTICAL CHEMISTRY, 85(12), 6136–6142.
MeSH headings : Cell Line, Tumor; Cell Separation / methods; Electrophoresis, Capillary / methods; Enzyme Activation / physiology; Flow Cytometry / methods; Humans; Protein Tyrosine Phosphatases / analysis; Protein Tyrosine Phosphatases / metabolism
TL;DR:
A fluorescent peptide substrate was used to measure dephosphorylation by protein tyrosine phosphatases (PTP) in cell lysates and single cells and to investigate the effect of environmental toxins on PTP activity in these systems, demonstrating the impact of these toxic exposures on cell signaling and the heterogeneity of response between cells.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2013 journal article
Measuring Activity in the Ubiquitin-Proteasome System: From Large Scale Discoveries to Single Cells Analysis
CELL BIOCHEMISTRY AND BIOPHYSICS, 67(1), 75–89.
author keywords: Ubiquitin; Proteasome; E3 Ligase; DUB; Reporter
TL;DR:
A recent study is presented highlighting the development of a novel degron-based substrate designed to overcome the limitations of current reporting techniques in measuring E3 ligase and proteasome activity in patient samples.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2013 journal article
Microfluidic Chemical Cytometry of Peptide Degradation in Single Drug-Treated Acute Myeloid Leukemia Cells
ANALYTICAL CHEMISTRY, 85(10), 4991–4997.
MeSH headings : Amino Acid Sequence; Antineoplastic Agents / pharmacology; Cell Line, Tumor; Humans; Kinetics; Leukemia, Myeloid, Acute / pathology; Microfluidic Analytical Techniques / methods; Peptides / chemistry; Peptides / metabolism; Proteolysis / drug effects
TL;DR:
This work used a robust microfluidic platform to explore the heterogeneity of enzyme activity in single cells treated with a chemotherapeutic drug and found a linear dependence of degradation rate on amount of substrate loaded superimposed upon substantial heterogeneity in peptide processing in response to inhibitor treatment.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2013 journal article
Micropallet arrays for the capture, isolation and culture of circulating tumor cells from whole blood of mice engrafted with primary human pancreatic adenocarcinoma
BIOSENSORS & BIOELECTRONICS, 54, 476–483.
author keywords: Microfabrication; Surface functionalization; Cell array; Circulating tumor cells; Cell sorting; Pancreatic cancer
MeSH headings : Adenocarcinoma / blood; Adenocarcinoma / pathology; Animals; Biosensing Techniques / instrumentation; Cell Line, Tumor; Cell Separation / instrumentation; Equipment Design; Female; Humans; Mice; Neoplastic Cells, Circulating / pathology; Pancreatic Neoplasms / blood; Pancreatic Neoplasms / pathology; Tissue Array Analysis / instrumentation
TL;DR:
Clinical utility of this technology was shown through the capture, isolation and successful culture of CTCs from the blood of mice engrafted with primary human pancreatic tumors and direct capture and isolation of living tumor cells from blood followed by analysis or culture will be a valuable tool for cancer cell characterization.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2013 journal article
Scalable synthesis of a biocompatible, transparent and superparamagnetic photoresist for microdevice fabrication
Journal of Micromechanics and Microengineering, 23(10).
Source: NC State University Libraries
Added: August 6, 2018
2013 journal article
beta-Turn sequences promote stability of peptide substrates for kinases within the cytosolic environment
Analyst [London], 138(15), 4305–4311.
Source: NC State University Libraries
Added: August 6, 2018
2012 journal article
Development of a Peptidase-Resistant Substrate for Single-Cell Measurement of Protein Kinase B Activation
ANALYTICAL CHEMISTRY, 84(16), 7195–7202.
MeSH headings : Amino Acid Sequence; Amino Acid Substitution; Arginine; Cytosol / metabolism; Drug Design; Enzyme Activation / drug effects; HeLa Cells; Humans; Oligopeptides / chemistry; Oligopeptides / metabolism; Oligopeptides / pharmacology; Peptide Hydrolases / metabolism; Phosphorylation; Protein Binding; Protein Kinase Inhibitors / chemistry; Protein Kinase Inhibitors / metabolism; Protein Kinase Inhibitors / pharmacology; Proteolysis; Proto-Oncogene Proteins c-akt / antagonists & inhibitors; Proto-Oncogene Proteins c-akt / metabolism; Single-Cell Analysis
TL;DR:
An iterative design strategy using three criteria was utilized to develop a peptidase-resistant substrate peptide for protein kinase B, finding widespread utility in both lysate-based and single-cell assays of kinase activity.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2012 journal article
Dissolution-guided wetting for microarray and microfluidic devices
Lab on a Chip, 12(17), 3036–3039.
Source: NC State University Libraries
Added: August 6, 2018
2012 journal article
Efficient division and sampling of cell colonies using microcup arrays
ANALYST, 138(1), 220–228.
MeSH headings : Animals; Cell Culture Techniques; Cell Separation / methods; Embryonic Stem Cells / cytology; Lasers; Mice; Microtechnology; Single-Cell Analysis; Time Factors; Tissue Array Analysis / methods
TL;DR:
A microengineered array to sample clonal colonies is described, envisioned as a means to rapidly establish cell lines using a destructive assay to identify desired clones.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
7. Affordable and Clean Energy
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2012 journal article
Laser-based directed release of array elements for efficient collection into targeted microwells
ANALYST, 138(3), 831–838.
MeSH headings : Cell Line, Tumor; Cell Separation; Cell Survival; Dimethylpolysiloxanes / chemistry; Green Fluorescent Proteins / genetics; Green Fluorescent Proteins / metabolism; HeLa Cells; Humans; Lasers; Tissue Array Analysis; Transfection
TL;DR:
An array of microfabricated, releasable, transparent micron-scale pedestals termed pallets and an array of microwells in poly(dimethylsiloxane) (PDMS) were mated to enable selective release and retrieval of individual cells.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2012 journal article
Metabolism of peptide reporters in cell lysates and single cells
Analyst [London], 137(13), 3028–3038.
Source: NC State University Libraries
Added: August 6, 2018
2012 review
Micro Total Analysis Systems: Fundamental Advances and Applications in the Laboratory, Clinic, and Field
[Review of ]. ANALYTICAL CHEMISTRY, 85(2), 451–472.
MeSH headings : Clinical Laboratory Techniques / instrumentation; Equipment Design; Laboratories; Microtechnology / instrumentation
TL;DR:
This review focuses on advances in the area of μTAS or “lab-on-a-chip” systems over the time span of May 2011 through September 2012 with a focus on applications in basic research, clinical medicine and field usage.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2012 journal article
Microfabricated Arrays for Splitting and Assay of Clonal Colonies
ANALYTICAL CHEMISTRY, 84(24), 10614–10620.
MeSH headings : Animals; Cell Separation / methods; Cells, Cultured; Clone Cells / cytology; Cloning, Molecular / methods; HeLa Cells; Humans; Mice; Microtechnology / methods; NIH 3T3 Cells
TL;DR:
miniaturization and its parallel format enabled the identification and generation of 12 target clones from a starting sample of only 3900 cells and required only 5 man hours over 11 days, in contrast to the traditional method which required 500,000 cells and generated only 5 target clones.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2012 journal article
Separation of fluorescently labeled phosphoinositides and sphingolipids by capillary electrophoresis
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 907, 79–86.
author keywords: Capillary electrophoresis; Sphingolipid; Phosphoinositide lipid; Phospholipid separation
MeSH headings : 1-Propanol / chemistry; Boron Compounds / chemistry; Cell Line, Tumor; Electrophoresis, Capillary / methods; Fluorescein / chemistry; Fluorescent Dyes / chemistry; Humans; Hydrogen-Ion Concentration; Limit of Detection; Phosphates / chemistry; Phosphatidylinositols / chemistry; Phosphatidylinositols / isolation & purification; Reproducibility of Results; Sphingolipids / chemistry; Sphingolipids / isolation & purification
TL;DR:
A simple and robust separation method for SFL and S1PFL and various metabolites of phosphoinositide-related signal transduction is expected to enable improved enzymatic assays for biological and clinical applications.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
14. Life Below Water
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2012 article
Trapping cells on a stretchable microwell array for single-cell analysis (vol 402, pg 1065, 2012)
Analytical and Bioanalytical Chemistry, Vol. 404, pp. 2519–2519.
Source: NC State University Libraries
Added: August 6, 2018
2011 journal article
Benchtop micromolding of polystyrene by soft lithography
Lab on a Chip, 11(18), 3089–3097.
Source: NC State University Libraries
Added: August 6, 2018
2011 journal article
Continuous analysis of dye-loaded, single cells on a microfluidic chip
Lab on a Chip, 11(7), 1333–1341.
Source: NC State University Libraries
Added: August 6, 2018
2011 journal article
Femtomole SHAPE Reveals Regulatory Structures in the Authentic XMRV RNA Genome
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 133(50), 20326–20334.
MeSH headings : Acetylation; Base Sequence; Electrophoresis, Capillary; Genome, Viral; Leukemia Virus, Murine / genetics; Molecular Sequence Data; Nucleic Acid Conformation; RNA, Viral / chemistry; RNA, Viral / genetics
TL;DR:
Using high-sensitivity SHAPE, the dimeric structure of the XMRV packaging domain is determined, dynamic interactions between the packaging domain RNA and viral nucleocapsid protein inside virion particles are examined, and the packaging signal for this virus is identified.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2011 journal article
Isolation and manipulation of living adherent cells by micromolded magnetic rafts
Biomicrofluidics, 5(3).
Source: NC State University Libraries
Added: August 6, 2018
2011 journal article
Kinetic analysis of PI3K reactions with fluorescent PIP2 derivatives
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 401(6), 1881–1888.
author keywords: PI3K assay; Fluorescent phosphatidylinositides; TLC analysis; Capillary electrophoresis
MeSH headings : Chromatography, Thin Layer / methods; Electrophoresis, Capillary / methods; Enzyme Inhibitors / pharmacology; Fluorescent Dyes / chemistry; Fluorescent Dyes / metabolism; Kinetics; Phosphatidylinositol 3-Kinase / metabolism; Phosphatidylinositol 4,5-Diphosphate / chemistry; Phosphatidylinositol 4,5-Diphosphate / metabolism; Phosphoinositide-3 Kinase Inhibitors; Sensitivity and Specificity
TL;DR:
Characterizations of two fluorescent PIP2 derivatives as reporters for PI3K enzymatic activity have laid the groundwork to systematically measure cellular PI3k activity, and have the potential to generate molecular fingerprints for diagnostic and therapeutic applications.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2011 review
Measuring enzyme activity in single cells
[Review of ]. TRENDS IN BIOTECHNOLOGY, 29(5), 222–230.
MeSH headings : Cells / metabolism; Cytological Techniques / methods; Electrophoresis, Capillary / methods; Enzymes / metabolism; Flow Cytometry / methods; Microscopy / methods
TL;DR:
Expansion of single-cell assays of enzyme activity to a wider range and larger number of enzymes will answer contemporary questions in proteomics and glycomics, specifically with respect to biological noise and cellular heterogeneity.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2011 review
Micro Total Analysis Systems for Cell Biology and Biochemical Assays
[Review of ]. ANALYTICAL CHEMISTRY, 84(2), 516–540.
MeSH headings : Animals; Biological Assay; Clinical Laboratory Techniques / instrumentation; Clinical Laboratory Techniques / trends; Humans; Microfluidic Analytical Techniques
TL;DR:
Application of µTAS in all of these areas continues to be highly interdisciplinary, utilizing techniques and strategies from almost every scientific field, and insures continued relevance to the biological community as well as a bright future.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2011 journal article
Patterning pallet arrays for cell selection based on high-resolution measurements of fluorescent biosensors
ANALYTICA CHIMICA ACTA, 696(1-2), 101–107.
author keywords: Pallet array; Cell separation; Patterned surfaces; FRET; Cell sorting; Micro-contact printing
MeSH headings : Animals; Biosensing Techniques / methods; Cell Line; Cell Separation / methods; Fibronectins / chemistry; Fluorescence Resonance Energy Transfer / methods; HeLa Cells; Humans; Mice; Tissue Array Analysis / methods; rhoA GTP-Binding Protein / metabolism
TL;DR:
The ability to perform spatially resolved measurements of fluorescence-based sensors on the pallet arrays should enable novel cell separations in which cell selection is based on complex cellular signaling properties.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
7. Affordable and Clean Energy
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2011 journal article
Polystyrene-coated micropallets for culture and separation of primary muscle cells
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 402(3), 1083–1091.
author keywords: Bioanalytical methods; Biomaterials; Cell systems; Single cell analysis
MeSH headings : Animals; Cell Adhesion; Cell Culture Techniques / instrumentation; Cell Proliferation; Cell Separation / instrumentation; Cells, Cultured; Dogs; Muscle Cells / cytology; Polystyrenes / chemistry; Surface Properties; Tissue Array Analysis / instrumentation
TL;DR:
Optimization of the microstructures’ surface properties was conducted for the culture of satellite cells, primary muscle cells for which improved cell isolation techniques are desired, and this micropallet coating strategy is readily adaptable to optimize the array surface for other types of primary cells.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2011 journal article
Sample transport and electrokinetic injection in a microchip device for chemical cytometry
ELECTROPHORESIS, 32(22), 3180–3187.
author keywords: Chemical cytometry; Electrokinetic injection bias; Microfluidics; Simulation
MeSH headings : Computer Simulation; Equipment Design; Microfluidic Analytical Techniques / instrumentation; Models, Chemical; Peptides / chemistry; Phosphatidylcholines / chemistry; Phosphatidylglycerols / chemistry; Pressure; Reproducibility of Results; Single-Cell Analysis / instrumentation; Single-Cell Analysis / methods
TL;DR:
It is found that increasing electric field strength and electrophoretic mobility result in improved injection and that COMSOL simulations accurately predict sample transport, indicating generally that device design must account for analyte characteristics and specifically that this device is suited to high‐mobility analytes.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2011 journal article
Single-cell analysis of phosphoinositide 3-kinase and phosphatase and tensin homolog activation
Faraday Discussions, 149, 187–200.
Source: NC State University Libraries
Added: August 6, 2018
2011 journal article
Trapping cells on a stretchable microwell array for single-cell analysis
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 402(3), 1065–1072.
author keywords: Bioanalytical methods; Cell systems/single-cell analysis; Biochips/high-throughput screening
MeSH headings : Animals; Calcium / metabolism; Cell Line; Dimethylpolysiloxanes / chemistry; Equipment Design; Ionomycin / metabolism; Single-Cell Analysis / economics; Single-Cell Analysis / instrumentation; Tissue Array Analysis / economics; Tissue Array Analysis / instrumentation
TL;DR:
A simple, low-cost microarray platform that can trap cells for dynamic single-cell analysis of mammalian cells and demonstrate the utility by multicolor analysis of trapped cells and monitoring in individual cells real-time calcium flux after exposure to the calcium ionophore ionomycin is reported.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2011 journal article
Use of a virtual wall valve in polydimethylsiloxane microfluidic devices for bioanalytical applications
Biomicrofluidics, 5(2).
Source: NC State University Libraries
Added: August 6, 2018
2010 journal article
Air-stable supported membranes for single-cell cytometry on PDMS microchips
Lab on a Chip, 10(7), 864–870.
Source: NC State University Libraries
Added: August 6, 2018
2010 journal article
Contact printing of arrayed microstructures
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 397(8), 3377–3385.
author keywords: Contact printing; Microfabrication; SU-8; Cell culture; Micropallet
MeSH headings : Acrylic Resins / chemistry; Animals; Biocompatible Materials / chemistry; Cell Adhesion; Cell Culture Techniques; Cell Line; Extracellular Matrix / chemistry; Humans; Mice; Polystyrenes / chemistry; Surface Properties; Tissue Array Analysis / instrumentation
TL;DR:
A novel contact printing method utilizing a sacrificial layer of polyacrylic acid (PAA) was developed to selectively modify the upper surfaces of arrayed microstructures to create an improved substrate for a cell-based microarray platform.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2010 journal article
In situ Roughening of Polymeric Microstructures
ACS APPLIED MATERIALS & INTERFACES, 2(4), 1086–1093.
author keywords: roughening; in situ; polymer; MEMS; microarray; printing; cell
MeSH headings : Aluminum Oxide / chemistry; Animals; Biocompatible Materials / chemistry; Cattle; Cell Adhesion; Cell Culture Techniques / methods; Cell Proliferation; Cyclic AMP / analogs & derivatives; Cyclic AMP / chemistry; Fibronectins / chemistry; Fluorescent Dyes / chemistry; Microscopy, Atomic Force / methods; Polymers / chemistry; Polystyrenes / chemistry; Serum Albumin / chemistry; Surface Properties
TL;DR:
Roughening of micrometer-scale surfaces with a particle slurry increased the adhesion of biomolecules as well as cells to microstructures with little to no damage to largescale arrays of the structures.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2010 journal article
Microcup Arrays for the Efficient Isolation and Cloning of Cells
ANALYTICAL CHEMISTRY, 82(8), 3161–3167.
MeSH headings : 3T3 Cells; Animals; Cell Separation / instrumentation; Cell Separation / methods; Clone Cells; HeLa Cells; Hematopoietic Stem Cells / cytology; Humans; Magnetics; Mice; Tissue Array Analysis
TL;DR:
Direct comparisons with cell isolation by fluorescence-activated cell sorting and magnetic-bead-based isolation systems demonstrated that the microcup cell isolation procedure yielded higher purity, yield, and viability than these standard technologies when separating samples with small numbers of cells.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
7. Affordable and Clean Energy
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2010 journal article
Microdevice to capture colon crypts for in vitro studies
Lab on a Chip, 10(12), 1596–1603.
Source: NC State University Libraries
Added: August 6, 2018
2010 journal article
Microelectrophoresis platform for fast serial analysis of single cells
Electrophoresis, 31(15), 2558–2565.
Source: NC State University Libraries
Added: August 6, 2018
2010 journal article
Micromolded arrays for separation of adherent cells
Lab on a Chip, 10(21), 2917–2924.
Source: NC State University Libraries
Added: August 6, 2018
2010 journal article
Microtable arrays for culture and isolation of cell colonies
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 398(6), 2595–2604.
author keywords: Microtable; Micropallet; Microfabrication; 1002F; SU-8; Cell separation; Cell array
MeSH headings : Cell Culture Techniques / instrumentation; Cell Culture Techniques / methods; Cell Separation / instrumentation; Cell Separation / methods; Culture Media; Equipment Design; HeLa Cells; Humans; Microarray Analysis / instrumentation; Microarray Analysis / methods; Microscopy, Electron, Scanning; Microscopy, Fluorescence; Surface Properties
TL;DR:
Three-dimensional structures were microfabricated by a novel two-step photolithography process to create a unique array platform for cell biology applications and proved an efficient method for isolating colonies from the arrays with 100% of selected colonies competent to expand following release from the array.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2010 journal article
Selection and Separation of Viable Cells Based on a Cell-Lethal Assay
ANALYTICAL CHEMISTRY, 83(1), 278–283.
MeSH headings : Animals; Cell Adhesion; Cell Culture Techniques; Cell Separation / instrumentation; Cell Separation / methods; Cell Survival; Enzyme Activation; Extracellular Signal-Regulated MAP Kinases / metabolism; Feasibility Studies; HeLa Cells; Humans; Mice; NIH 3T3 Cells; Tissue Array Analysis
TL;DR:
These microarrays enabling clonal colony segmentation permitted sampling and manipulation of the colonies at very early times and at small cell numbers to reduce reagent, time, and manpower requirements.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2010 journal article
Transparent magnetic photoresists for bioanalytical applications
BIOMATERIALS, 31(33), 8810–8817.
author keywords: Magnetism; Photolithography; Biocomaptibility; Micropatterning; MTT assay; Cell adhesion
MeSH headings : Absorption; Animals; Biomedical Technology / instrumentation; Cell Line; Cell Proliferation; Cell Separation; Colorimetry; Ferric Compounds / chemistry; Humans; Magnetics / instrumentation; Nanoparticles / ultrastructure; Photochemistry / instrumentation
TL;DR:
Uniformly transparent magnetic photoresists were developed for microdevices that require high transparency as well as consistent magnetism across the structure and should find wide use in the fabrication of microstructures for bioanalytical technologies.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
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