Works (97)

Updated: August 29th, 2023 11:25

2021 journal article

Hyperglycemia minimally alters primary self-renewing human colonic epithelial cells while TNF alpha-promotes severe intestinal epithelial dysfunction

INTEGRATIVE BIOLOGY, 13(6), 139–152.

By: J. Dutton n, S. Hinman*, R. Kim*, P. Attayek n, M. Maurer n, C. Sims*, N. Allbritton*

author keywords: hyperglycemia; glucose; TNF alpha; colon; large intestine; intestinal epithelium; primary tissue
MeSH headings : Colon; Epithelial Cells; Humans; Hyperglycemia; Intestinal Mucosa; Tumor Necrosis Factor-alpha
Source: Web Of Science
Added: July 26, 2021

2021 journal article

Magnetically-propelled fecal surrogates for modeling the impact of solid-induced shear forces on primary colonic epithelial cells

BIOMATERIALS, 276.

author keywords: Colon; Intestine; Primary cells; Shear force; Magnetic; Hydrogel
MeSH headings : Colon; Epithelial Cells; Feces; Humans; Intestinal Mucosa; Muscle Contraction
Source: Web Of Science
Added: September 7, 2021

2021 article

Stem/Proliferative and Differentiated Cells within Primary Murine Colonic Epithelium Display Distinct Intracellular Free Ca2+ Signal Codes

Mestril, S., Kim, R., Hinman, S. S., Gomez, S. M., & Allbritton, N. L. (2021, September 12). ADVANCED HEALTHCARE MATERIALS.

By: S. Mestril n, R. Kim*, S. Hinman*, S. Gomez n & N. Allbritton*

author keywords: Ca2+; colon; hydrogels; intestine; primary cells; stiffness
MeSH headings : Animals; Cell Differentiation; Colon; Epithelium; Intestinal Mucosa; Mice; Signal Transduction
Source: Web Of Science
Added: September 20, 2021

2020 journal article

An in vitro intestinal platform with a self-sustaining oxygen gradient to study the human gut/microbiome interface

BIOFABRICATION, 12(1).

By: R. Kim n, P. Attayek n, Y. Wang*, K. Furtado*, R. Tamayo*, C. Sims*, N. Allbritton n

author keywords: intestine-on-chip; oxygen gradient; microbiome; intestinal crypt
MeSH headings : Bacteria / classification; Bacteria / growth & development; Bacteria / isolation & purification; Bacteria / metabolism; Cell Proliferation; Cells, Cultured; Colon / cytology; Colon / metabolism; Colon / microbiology; Epithelial Cells / cytology; Epithelial Cells / metabolism; Gastrointestinal Microbiome; Humans; In Vitro Techniques / methods; Intestines; Models, Biological; Oxygen / metabolism; Stem Cells / cytology; Stem Cells / metabolism
Source: Web Of Science
Added: December 16, 2019

2020 journal article

Assay and Isolation of Single Proliferating CD4+Lymphocytes Using an Automated Microraft Array Platform

IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING, 67(8), 2166–2175.

By: C. LaBelle n, R. Zhang*, P. Armistead* & N. Allbritton n

author keywords: Immune system; Media; Optical filters; Magnetic resonance imaging; Microscopy; Sorting; Cancer; Microarrays; lymphocyte; proliferation; cell sorting; immunology
MeSH headings : CD4-Positive T-Lymphocytes; Cell Separation; Cells, Cultured; Humans; Lymphocyte Activation
Source: Web Of Science
Added: August 10, 2020

2020 journal article

Automated platform for cell selection and separation based on four-dimensional motility and matrix degradation

ANALYST, 145(7), 2731–2742.

By: H. Nowotarski*, P. Attayek n & N. Allbritton n

MeSH headings : Automation; Cell Line, Tumor; Cell Movement; Cell Separation / methods; Collagen / chemistry; Humans; Image Processing, Computer-Assisted; Microscopy, Confocal; Tissue Array Analysis
Source: Web Of Science
Added: May 8, 2020

2020 review

Image-Based Live Cell Sorting

[Review of ]. TRENDS IN BIOTECHNOLOGY, 39(6), 613–623.

By: C. LaBelle n, A. Massaro*, B. Cortes-Llanos*, C. Sims* & N. Allbritton*

MeSH headings : Cell Physiological Phenomena; Cell Separation / instrumentation; Cell Separation / methods; Microfluidics
Source: Web Of Science
Added: June 10, 2021

2020 journal article

Microraft array-based platform for sorting of viable microcolonies based on cell-lethal immunoassay of intracellular proteins in microcolony biopsies

ANALYST, 145(7), 2649–2660.

By: N. Smiddy*, M. DiSalvo n, J. Allbritton-King n & N. Allbritton*

MeSH headings : Animals; Cell Line, Tumor; Humans; Image Processing, Computer-Assisted; Immunoassay / instrumentation; Immunoassay / methods; Leukemia, Basophilic, Acute / metabolism; Leukemia, Basophilic, Acute / pathology; Magnetics; Microarray Analysis; Microscopy, Fluorescence; Phosphorylation; Rats; STAT3 Transcription Factor / metabolism
Source: Web Of Science
Added: May 8, 2020

2020 journal article

Pooled CRISPR screens with imaging on microraft arrays reveals stress granule-regulatory factors

NATURE METHODS, 17(6), 636-+.

By: E. Wheeler*, A. Vu*, J. Einstein*, M. DiSalvo n, N. Ahmed*, E. Van Nostrand*, A. Shishkin*, W. Jin*, N. Allbritton n, G. Yee

MeSH headings : CRISPR-Cas Systems / genetics; Clustered Regularly Interspaced Short Palindromic Repeats / genetics; Cytoplasm / metabolism; Humans; Machine Learning; Microscopy, Confocal / methods; Oxidative Stress / genetics; Protein Aggregates / genetics; RNA, Guide, Kinetoplastida / genetics; RNA-Binding Proteins / genetics; Tissue Array Analysis / methods
Source: Web Of Science
Added: May 26, 2020

2020 review

Preserving single cells in space and time for analytical assays

[Review of ]. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 122.

By: L. Gallion*, M. Anttila*, D. Abraham*, A. Proctor* & N. Allbritton n

author keywords: Laboratory medicine; Single-cell analysis; Sample preparation; Fixation; Preservation; Signaling; Analytical chemistry; Bioanalytical chemistry; Clinical assays
Source: Web Of Science
Added: February 10, 2020

2019 journal article

"Fix and assay": separating in-cellulo sphingolipid reactions from analytical assay in time and space using an aldehyde-based fixative

ANALYST, 144(3), 961–971.

By: A. Proctor* & N. Allbritton n

MeSH headings : Aldehydes / chemistry; Aldehydes / metabolism; Biological Assay / methods; Electrophoresis, Capillary / methods; Humans; K562 Cells; Single-Cell Analysis / methods; Sphingolipids / metabolism; Tissue Fixation / methods; U937 Cells
Source: Web Of Science
Added: February 18, 2019

2019 journal article

Automated sensing and splitting of stem cell colonies on microraft arrays

APL BIOENGINEERING, 3(3).

By: M. DiSalvo n, N. Smiddy* & N. Allbritton n

Source: Web Of Science
Added: October 21, 2019

2019 personal communication

Building a Thick Mucus Hydrogel Layer to Improve the Physiological Relevance of In Vitro Primary Colonic Epithelial Models

By: Y. Wang*, R. Kim n, C. Sims* & N. Allbritton n

MeSH headings : Cell Culture Techniques / methods; Colon / metabolism; Humans; Hydrogels / metabolism; Intestinal Mucosa / metabolism; Mucus / metabolism; Mucus / physiology
Source: Web Of Science
Added: December 16, 2019

2019 journal article

Evaluation of human primary intestinal monolayers for drug metabolizing capabilities

JOURNAL OF BIOLOGICAL ENGINEERING, 13(1).

By: J. Speer*, Y. Wang*, J. Fallon n, P. Smith n & N. Allbritton n

author keywords: Drug metabolism; Stiffness; Extracellular matrix; Small intestine
Source: Web Of Science
Added: November 18, 2019

2019 journal article

Molecular transport through primary human small intestinal monolayers by culture on a collagen scaffold with a gradient of chemical cross-linking

JOURNAL OF BIOLOGICAL ENGINEERING, 13.

By: J. Speer*, D. Gunasekara n, Y. Wang*, J. Fallon*, P. Attayek n, P. Smith*, C. Sims*, N. Allbritton n

author keywords: Intestinal transport; Stiffness; Extracellular matrix; Small intestine
Source: Web Of Science
Added: May 13, 2019

2019 journal article

Photopatterned Membranes and Chemical Gradients Enable Scalable Phenotypic Organization of Primary Human Colon Epithelial Models

ANALYTICAL CHEMISTRY, 91(23), 15240–15247.

By: S. Hinman*, Y. Wang* & N. Allbritton n

MeSH headings : Cells, Cultured; Colon / cytology; Epithelial Cells / cytology; Humans; Microscopy, Fluorescence; Optical Imaging; Phenotype; Photochemical Processes; Polytetrafluoroethylene / chemistry
Source: Web Of Science
Added: December 30, 2019

2019 review

Primary Cell-Derived Intestinal Models: Recapitulating Physiology

[Review of ]. TRENDS IN BIOTECHNOLOGY, 37(7), 744–760.

By: J. Dutton n, S. Hinman*, R. Kim n, Y. Wang* & N. Allbritton n

MeSH headings : Cell Culture Techniques / methods; Cell Culture Techniques / trends; Cells, Cultured; Humans; Intestines / physiology; Models, Biological; Organoids / physiology; Tissue Engineering / methods; Tissue Engineering / trends
Source: Web Of Science
Added: July 1, 2019

2019 article

Selection and optimization of enzyme reporters for chemical cytometry

CHEMICAL AND SYNTHETIC BIOLOGY APPROACHES TO UNDERSTAND CELLULAR FUNCTIONS - PT B, Vol. 622, pp. 221–248.

By: A. Proctor*, Q. Wang*, D. Lawrence* & N. Allbritton n

MeSH headings : Animals; Enzyme Assays / methods; Humans; Kinetics; Peptide Hydrolases / metabolism; Peptide Library; Peptides / chemical synthesis; Peptides / chemistry; Peptides / metabolism; Single-Cell Analysis / methods; Solid-Phase Synthesis Techniques / methods; Substrate Specificity
Source: Web Of Science
Added: December 30, 2019

2018 journal article

A Monolayer of Primary Colonic Epithelium Generated on a Scaffold with a Gradient of Stiffness for Drug Transport Studies

ANALYTICAL CHEMISTRY, 90(22), 13331–13340.

By: D. Gunasekara n, J. Speer*, Y. Wang*, D. Nguyen*, M. Reed*, N. Smiddy*, J. Parker*, J. Fallon* ...

MeSH headings : Animals; Atenolol / metabolism; Biological Transport / physiology; Caco-2 Cells; Chickens; Collagen / chemistry; Colon / physiology; Epithelium / physiology; Humans; Mice; Propranolol / metabolism; Rhodamine 123 / metabolism; Riboflavin / metabolism; Tissue Engineering / methods
Source: Web Of Science
Added: December 10, 2018

2018 journal article

Analysis of Interleukin 8 Secretion by a Stem-Cell-Derived Human-Intestinal-Epithelial-Monolayer Platform

ANALYTICAL CHEMISTRY, 90(19), 11523–11530.

By: Y. Wang*, R. Kim n, S. Hwang*, J. Dutton n, C. Sims* & N. Allbritton n

MeSH headings : Cells, Cultured; Chemokine CCL2 / analysis; Enzyme-Linked Immunosorbent Assay; Humans; Interleukin-8 / analysis; Interleukin-8 / metabolism; Intestinal Mucosa / cytology; Intestinal Mucosa / drug effects; Intestinal Mucosa / metabolism; Porosity; Stem Cells / cytology; Tumor Necrosis Factor-alpha / pharmacology
Source: Web Of Science
Added: October 19, 2018

2018 review

Bioengineered systems and designer matrices that recapitulate the intestinal stem cell niche

[Review of ]. Cellular and Molecular Gastroenterology and Hepatology, 5(3), 440-.

By: Y. Wang, R. Kim, S. Hinman, B. Zwarycz, S. Magness & N. Allbritton

Source: NC State University Libraries
Added: August 6, 2018

2018 journal article

Characterization of Tensioned PDMS Membranes for Imaging Cytometry on Microraft Arrays

ANALYTICAL CHEMISTRY, 90(7), 4792–4800.

By: M. DiSalvo n, D. Harris*, S. Kantesaria n, A. Pena*, J. Allbritton-King n, J. Cole n, N. Allbritton n

MeSH headings : Automation; Cell Line, Tumor; Dimethylpolysiloxanes / chemistry; Flow Cytometry; Humans; Lung Neoplasms / diagnostic imaging; Microarray Analysis; Optical Imaging; Surface Properties
Sources: Web Of Science, ORCID
Added: August 6, 2018

2018 journal article

Formation of Human Colonic Crypt Array by Application of Chemical Gradients Across a Shaped Epithelial Monolayer

CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY, 5(2), 113–130.

By: Y. Wang*, R. Kim n, D. Gunasekara*, M. Reed*, M. DiSalvo n, D. Nguyen*, S. Bultman*, C. Sims*, S. Magness n, N. Allbritton n

author keywords: Intestinal Epithelial Cells; Intestine-On-A-Chip; Stem Cell Niche; Polarized Crypt
Source: Web Of Science
Added: August 6, 2018

2018 journal article

LITE microscopy: Tilted light-sheet excitation of model organisms offers high resolution and low photobleaching

JOURNAL OF CELL BIOLOGY, 217(5), 1869–1882.

By: T. Fadero*, T. Gerbich*, K. Rana*, A. Suzuki*, M. DiSalvo n, K. Schaefer*, J. Heppert*, T. Boothby* ...

MeSH headings : Animals; Arabidopsis / cytology; Cell Line; Cell Nucleus / metabolism; Fluorescence; Fungi / cytology; Humans; Imaging, Three-Dimensional; Light; Microscopy, Fluorescence / methods; Models, Biological; Photobleaching; Reproducibility of Results; Time-Lapse Imaging
Source: Web Of Science
Added: August 6, 2018

2018 journal article

N-Gemini peptides: cytosolic protease resistance via N-terminal dimerization of unstructured peptides

CHEMICAL COMMUNICATIONS, 54(2), 204–207.

By: E. Fayer*, W. Gilliland*, J. Ramsey n, N. Allbritton n & M. Waters*

MeSH headings : Cell Line; Cytosol / enzymology; Dimerization; HeLa Cells; Humans; Molecular Structure; Peptide Hydrolases / metabolism; Peptides / chemical synthesis; Peptides / chemistry; Peptides / pharmacology; Protease Inhibitors / chemistry; Protease Inhibitors / pharmacology
Source: Web Of Science
Added: August 6, 2018

2018 journal article

Nonsteroidal Anti-Inflammatory Drug -Induced Leaky Gut Modeled Using Polarized Monolayers of Primary Human Intestinal Epithelial Cells

ACS INFECTIOUS DISEASES, 4(1), 46–52.

By: A. Bhatt*, D. Gunasekara*, J. Speer*, M. Reed*, A. Pena*, B. Midkiff*, S. Magness n, S. Bultman*, N. Allbritton n, M. Redinbo*

author keywords: small intestine; leaky gut; bacterial translocation; NSAIDs; mitochondria; superoxide
MeSH headings : Anti-Inflammatory Agents, Non-Steroidal / adverse effects; Cell Membrane Permeability / drug effects; Epithelial Cells / drug effects; Epithelial Cells / metabolism; Humans; Intestinal Mucosa / drug effects; Intestinal Mucosa / metabolism; Mitochondria / drug effects; Mitochondria / metabolism
Source: Web Of Science
Added: August 6, 2018

2017 journal article

A microengineered collagen scaffold for generating a polarized crypt-villus architecture of human small intestinal epithelium

BIOMATERIALS, 128, 44–55.

By: Y. Wang*, D. Gunasekara*, M. Reed*, M. DiSalvo n, S. Bultman*, C. Sims*, S. Magness n, N. Allbritton n

author keywords: Scaffold; Microfabrication; Intestine; Stem cell; Crypt; Villus
MeSH headings : Animals; Cell Compartmentation; Cell Differentiation / drug effects; Cell Movement / drug effects; Cell Proliferation / drug effects; Collagen / pharmacology; Cross-Linking Reagents / pharmacology; Humans; Hydrogel, Polyethylene Glycol Dimethacrylate / pharmacology; Intestinal Mucosa / drug effects; Intestinal Mucosa / physiology; Intestine, Small / drug effects; Intestine, Small / physiology; Mice; Organoids / drug effects; Porosity; Rats; Tissue Culture Techniques; Tissue Engineering / methods; Tissue Scaffolds / chemistry
Source: Web Of Science
Added: August 6, 2018

2017 journal article

Automated microraft platform to identify and collect non-adherent cells successfully gene-edited with CRISPR-Cas9

BIOSENSORS & BIOELECTRONICS, 91, 175–182.

By: P. Attayek n, J. Waugh*, S. Hunsucker*, P. Grayeski*, C. Sims*, P. Armistead*, N. Allbritton n

author keywords: Microraft; Cell array; CRISPR-Cas9; Cytometry; Cell sorting
MeSH headings : Biosensing Techniques / instrumentation; Biosensing Techniques / methods; CRISPR-Cas Systems; Equipment Design; Genes, Reporter; Green Fluorescent Proteins / genetics; Humans; Image Processing, Computer-Assisted; K562 Cells; Leukemia / genetics; Mutation; Splicing Factor U2AF / genetics; Tissue Array Analysis / instrumentation; Tissue Array Analysis / methods; Transfection
Source: Web Of Science
Added: August 6, 2018

2017 journal article

Chemical fixation to arrest phospholipid signaling for chemical cytometry

JOURNAL OF CHROMATOGRAPHY A, 1523, 97–106.

By: A. Proctor*, C. Sims* & N. Allbritton n

author keywords: Capillary electrophoresis; Phospholipid; Phospholipase C; Diacylglycerol; Chemical cytometry; Chemical fixation
MeSH headings : Electrophoresis, Capillary; Signal Transduction; Tissue Fixation / methods; Type C Phospholipases / metabolism
Source: Web Of Science
Added: August 6, 2018

2017 journal article

Required hydrophobicity of fluorescent reporters for phosphatidylinositol family of lipid enzymes

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 409(29), 6781–6789.

By: J. Waybright*, W. Huang*, A. Proctor*, X. Wang*, N. Allbritton* & Q. Zhang*

author keywords: Fluorescent reporter; Chemical cytometry; Lipid enzyme; Phosphatidylinositol
MeSH headings : Enzymes / metabolism; Fluorescent Dyes / chemistry; Hydrophobic and Hydrophilic Interactions; Lipids / chemistry; Liposomes / metabolism; Phosphatidylinositols / metabolism
Source: Web Of Science
Added: August 6, 2018

2017 journal article

Self-renewing monolayer of primary colonic or rectal epithelial cells

Cellular and Molecular Gastroenterology and Hepatology, 4(1), 165-.

By: Y. Wang, M. DiSalvo, D. Gunasekara, J. Dutton, A. Proctor, M. Lebhar, I. Williamson, J. Speer ...

Source: NC State University Libraries
Added: August 6, 2018

2016 journal article

An Integrated Chemical Cytometry Method: Shining a Light on Akt Activity in Single Cells

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 55(42), 13095–13098.

By: E. Mainz*, Q. Wang*, D. Lawrence* & N. Allbritton n

author keywords: biosensors; chemical cytometry; kinase activity probes; photochemistry; single-cell analysis
MeSH headings : Biosensing Techniques; Cell Line, Tumor; Flow Cytometry; Humans; Proto-Oncogene Proteins c-akt / metabolism; Single-Cell Analysis
Source: Web Of Science
Added: August 6, 2018

2016 journal article

Co-fabrication of chitosan and epoxy photoresist to form microwell arrays with permeable hydrogel bottoms

BIOMATERIALS, 74, 77–88.

By: D. Ornoff*, Y. Wang*, A. Proctor*, A. Shah* & N. Allbritton n

author keywords: Hydrogel; Microwell array; Chitosan; Microfabrication
MeSH headings : Animals; Cell Line; Cell Proliferation; Cell Survival; Chitosan / chemistry; Epoxy Compounds / chemistry; Hydrogels / chemistry; Mice; Microscopy, Electron, Scanning; Permeability
Source: Web Of Science
Added: August 6, 2018

2016 journal article

Development of a protease-resistant reporter to quantify BCR-ABL activity in intact cells

ANALYST, 141(21), 6008–6017.

By: A. Proctor*, I. Zigoneanu n, Q. Wang*, C. Sims*, D. Lawrence* & N. Allbritton n

MeSH headings : Animals; Cell Line, Tumor; Fusion Proteins, bcr-abl / metabolism; Leukemia, Myelogenous, Chronic, BCR-ABL Positive; Mice; Peptide Hydrolases / metabolism; Peptides / metabolism; Phosphorylation; Protein-Tyrosine Kinases / metabolism; Substrate Specificity
Source: Web Of Science
Added: August 6, 2018

2016 journal article

Identification and isolation of antigen-specific cytotoxic T lymphocytes with an automated microraft sorting system

INTEGRATIVE BIOLOGY, 8(12), 1208–1220.

By: P. Attayek n, S. Hunsucker*, C. Sims*, N. Allbritton n & P. Armistead*

MeSH headings : Antigens, Viral / immunology; CD4-Positive T-Lymphocytes / immunology; CD8-Positive T-Lymphocytes / immunology; Cell Separation; Cells, Cultured; Epitope Mapping / instrumentation; Equipment Design; Equipment Failure Analysis; Humans; Immunoassay / instrumentation; Killer Cells, Natural / immunology; Lab-On-A-Chip Devices; T-Lymphocytes, Cytotoxic / immunology; Tissue Array Analysis / instrumentation
Source: Web Of Science
Added: August 6, 2018

2016 journal article

In vitro polarization of colonoids to create an intestinal stem cell compartment

PLoS One, 11(4).

By: P. Attayek, A. Ahmad, Y. Wang, I. Williamson, C. Sims, S. Magness, N. Allbritton

Source: NC State University Libraries
Added: August 6, 2018

2016 journal article

Rational Design of a Dephosphorylation-Resistant Reporter Enables Single-Cell Measurement of Tyrosine Kinase Activity

ACS CHEMICAL BIOLOGY, 11(2), 355–362.

By: A. Turner, M. Lebhae, A. Proctor, Q. Wang, D. Lawrence & N. Allbritton n

MeSH headings : Cell Line, Tumor; Enzyme Assays / methods; ErbB Receptors / chemistry; ErbB Receptors / metabolism; Humans; Phosphorylation; Single-Cell Analysis / methods; Tetrahydroisoquinolines / chemistry; Tetrahydroisoquinolines / metabolism; Tyrosine / chemistry; Tyrosine / metabolism
Source: Web Of Science
Added: August 6, 2018

2016 journal article

Single Cell Chemical Cytometry of Akt Activity in Rheumatoid Arthritis and Normal Fibroblast-like Synoviocytes in Response to Tumor Necrosis Factor alpha

ANALYTICAL CHEMISTRY, 88(15), 7786–7792.

By: E. Mainz*, D. Serafin*, T. Nguyen*, T. Tarrant*, C. Sims* & N. Allbritton*

MeSH headings : Arthritis, Rheumatoid / metabolism; Arthritis, Rheumatoid / pathology; Cells, Cultured; Chromones / pharmacology; Electrophoresis, Capillary; Fibroblasts / cytology; Humans; Insulin / pharmacology; Morpholines / pharmacology; Phosphorylation; Proto-Oncogene Proteins c-akt / antagonists & inhibitors; Proto-Oncogene Proteins c-akt / genetics; Proto-Oncogene Proteins c-akt / metabolism; RNA Interference; RNA, Small Interfering / metabolism; Receptors, Tumor Necrosis Factor, Type I / metabolism; Signal Transduction / drug effects; Single-Cell Analysis; Synoviocytes / cytology; Synoviocytes / drug effects; Synoviocytes / metabolism; Tumor Necrosis Factor-alpha / pharmacology
Source: Web Of Science
Added: August 6, 2018

2016 journal article

Single-cell functional analysis of parathyroid adenomas reveals distinct classes of calcium sensing behaviour in primary hyperparathyroidism

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 20(2), 351–359.

By: J. Koh*, J. Hogue*, Y. Wang n, M. DiSalvo n, N. Allbritton n, Y. Shi*, J. Olson*, J. Sosa*

author keywords: hyperparathyroidism; parathyroid; adenoma; calcium; single-cell analysis
MeSH headings : Adenoma / metabolism; Calcium / metabolism; Calcium Signaling / physiology; Cell Line; Humans; Hyperparathyroidism, Primary / metabolism; Parathyroid Neoplasms / metabolism; Receptors, Calcium-Sensing / metabolism; Single-Cell Analysis / methods
Source: Web Of Science
Added: August 6, 2018

2015 journal article

Analysis of sphingosine kinase activity in single natural killer cells from peripheral blood

INTEGRATIVE BIOLOGY, 7(4), 392–401.

By: A. Dickinson*, M. Meyer*, E. Pawlak*, S. Gomez n, I. Jaspers* & N. Allbritton n

MeSH headings : Cells, Cultured; Enzyme Activation; Humans; Killer Cells, Natural / enzymology; Phosphotransferases (Alcohol Group Acceptor) / blood; Phosphotransferases (Alcohol Group Acceptor) / chemistry
Source: Web Of Science
Added: August 6, 2018

2015 journal article

Array-Based Platform To Select, Release, and Capture Epstein-Barr Virus-Infected Cells Based on Intercellular Adhesion

ANALYTICAL CHEMISTRY, 87(24), 12281–12289.

By: P. Attayek*, S. Hunsucker*, Y. Wang*, C. Sims*, P. Armistead* & N. Allbritton*

MeSH headings : Cell Adhesion; Cell Separation / instrumentation; Cell Separation / methods; Cell Survival; Dimethylpolysiloxanes / chemistry; Epstein-Barr Virus Nuclear Antigens / genetics; Epstein-Barr Virus Nuclear Antigens / metabolism; Herpesvirus 4, Human / physiology; Humans; K562 Cells; Microarray Analysis / instrumentation; Nylons / chemistry; Particle Size; Single-Cell Analysis / instrumentation; Surface Properties; Tumor Cells, Cultured; Viral Proteins / genetics; Viral Proteins / metabolism
Source: Web Of Science
Added: August 6, 2018

2015 journal article

Magnetic Alignment of Microelements Containing Cultured Neuronal Networks for High-Throughput Screening

JOURNAL OF BIOMOLECULAR SCREENING, 20(9), 1091–1100.

By: K. Gordon n, Y. Wang*, N. Allbritton n & A. Taylor n

author keywords: high-throughput screening; primary neurons; magnetic centering; microfabrication; finite element analysis; microraft arrays
MeSH headings : Animals; Cell Survival; Cells, Cultured; Drug Evaluation, Preclinical; High-Throughput Screening Assays / instrumentation; High-Throughput Screening Assays / methods; Magnetic Phenomena; Nerve Net / cytology; Neurons / drug effects; Neurons / physiology; Rats, Sprague-Dawley
Source: Web Of Science
Added: August 6, 2018

2015 journal article

Optimizing Wnt-3a and R-spondin1 concentrations for stem cell renewal and differentiation in intestinal organoids using a gradient-forming microdevice

RSC ADVANCES, 5(91), 74881–74891.

By: A. Ahmad n, Y. Wang*, C. Sims*, S. Magness* & N. Allbritton n

Source: Web Of Science
Added: August 6, 2018

2015 journal article

Pronase E-Based Generation of Fluorescent Peptide Fragments: Tracking Intracellular Peptide Fate in Single Cells

ANALYTICAL CHEMISTRY, 87(15), 7987–7995.

By: E. Mainz, N. Dobes & N. Allbritton*

MeSH headings : Amino Acid Sequence; Fluorescence; Intracellular Space / metabolism; Models, Biological; Molecular Sequence Data; Peptide Fragments / biosynthesis; Peptide Fragments / metabolism; Pronase / metabolism; Proteolysis; Single-Cell Analysis / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Source: Web Of Science
Added: August 6, 2018

2015 journal article

Separation of peptide fragments of a protein kinase C substrate fused to a beta-hairpin by capillary electrophoresis

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 407(30), 8999–9008.

By: I. Zigoneanu n, C. Sims* & N. Allbritton n

author keywords: Capillary electrophoresis; beta-Hairpin; Peptide separation; PKC; Protease resistance; Reporter
MeSH headings : Electrophoresis, Capillary / methods; Molecular Structure; Peptide Fragments / chemistry; Peptide Fragments / isolation & purification; Phosphorylation; Protein Kinase C / chemistry
Source: Web Of Science
Added: August 6, 2018

2014 journal article

An Immature Retroviral RNA Genome Resembles a Kinetically Trapped Intermediate State

JOURNAL OF VIROLOGY, 88(11), 6061–6068.

By: J. Grohman*, R. Gorelick*, S. Kottegoda*, N. Allbritton n, A. Rein* & K. Weeks*

MeSH headings : Acylation; DNA Primers / genetics; Dimerization; Electrophoresis, Capillary; Genome, Viral / genetics; Models, Molecular; Moloney murine leukemia virus / genetics; RNA, Viral / genetics; Virion / genetics; Virion / growth & development
Source: Web Of Science
Added: August 6, 2018

2014 journal article

Dynamics and evolution of beta-catenin-dependent Wnt signaling revealed through massively parallel clonogenic screening

INTEGRATIVE BIOLOGY, 6(7), 673–684.

By: P. Shah n, M. Walker*, C. Sims*, M. Major* & N. Allbritton n

MeSH headings : Cell Line, Tumor; Clone Cells; Humans; Image Processing, Computer-Assisted; Kinetics; Luminescent Proteins / chemistry; Luminescent Proteins / metabolism; Microscopy, Fluorescence / methods; Wnt Proteins / metabolism; Wnt Signaling Pathway; beta Catenin / metabolism
Source: Web Of Science
Added: August 6, 2018

2014 journal article

Ex Vivo Chemical Cytometric Analysis of Protein Tyrosine Phosphatase Activity in Single Human Airway Epithelial Cells

ANALYTICAL CHEMISTRY, 86(2), 1291–1297.

By: R. Phillips*, L. Dailey*, E. Bair*, J. Samet* & N. Allbritton n

MeSH headings : Bronchi / cytology; Bronchi / drug effects; Bronchi / enzymology; Cell Line; Electrophoresis, Capillary; Enzyme Inhibitors / pharmacology; Epithelial Cells / cytology; Epithelial Cells / drug effects; Epithelial Cells / enzymology; Half-Life; Humans; Hydrolysis; Microinjections; Naphthoquinones / pharmacology; Phosphoproteins / administration & dosage; Phosphoproteins / metabolism; Primary Cell Culture; Protein Tyrosine Phosphatases / analysis; Protein Tyrosine Phosphatases / antagonists & inhibitors; Protein Tyrosine Phosphatases / metabolism; Single-Cell Analysis; Vanadates / pharmacology
Source: Web Of Science
Added: August 6, 2018

2014 journal article

Measurement of Protein Kinase B Activity in Single Primary Human Pancreatic Cancer Cells

ANALYTICAL CHEMISTRY, 86(9), 4573–4580.

By: A. Proctor*, S. Herrera-Loeza*, Q. Wang*, D. Lawrence*, J. Yeh* & N. Allbritton n

MeSH headings : Adenocarcinoma / enzymology; Adenocarcinoma / pathology; Blotting, Western; Cell Line, Tumor; Humans; Pancreatic Neoplasms / enzymology; Pancreatic Neoplasms / pathology; Proto-Oncogene Proteins c-akt / metabolism
Source: Web Of Science
Added: August 6, 2018

2014 journal article

Micropallet arrays for the capture, isolation and culture of circulating tumor cells from whole blood of mice engrafted with primary human pancreatic adenocarcinoma

BIOSENSORS & BIOELECTRONICS, 54, 476–483.

By: P. Gach*, P. Attayek n, R. Whittlesey*, J. Yeh* & N. Allbritton n

author keywords: Microfabrication; Surface functionalization; Cell array; Circulating tumor cells; Cell sorting; Pancreatic cancer
MeSH headings : Adenocarcinoma / blood; Adenocarcinoma / pathology; Animals; Biosensing Techniques / instrumentation; Cell Line, Tumor; Cell Separation / instrumentation; Equipment Design; Female; Humans; Mice; Neoplastic Cells, Circulating / pathology; Pancreatic Neoplasms / blood; Pancreatic Neoplasms / pathology; Tissue Array Analysis / instrumentation
Source: Web Of Science
Added: August 6, 2018

2014 journal article

Response of single leukemic cells to peptidase inhibitor therapy across time and dose using a microfluidic device

INTEGRATIVE BIOLOGY, 6(2), 164–174.

By: M. Kovarik*, A. Dickinson*, P. Roy*, R. Poonnen*, J. Fine* & N. Allbritton*

MeSH headings : Cell Survival / drug effects; Dose-Response Relationship, Drug; Glycine / administration & dosage; Glycine / analogs & derivatives; Glycine / pharmacology; Humans; Hydroxamic Acids / administration & dosage; Hydroxamic Acids / pharmacology; Leukemia, Myeloid, Acute / drug therapy; Leukemia, Myeloid, Acute / enzymology; Longitudinal Studies; Microscopy, Fluorescence; Peptide Hydrolases / metabolism; Protease Inhibitors / administration & dosage; Protease Inhibitors / pharmacology; Regression Analysis; U937 Cells
Source: Web Of Science
Added: August 6, 2018

2014 journal article

Single-cell sphingosine kinase activity measurements in primary leukemia

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 406(27), 7027–7036.

By: A. Dickinson*, S. Hunsucker*, P. Armistead* & N. Allbritton*

author keywords: Single-cell analysis; Sphingosine kinase; Capillary electrophoresis; Leukemia; Primary cells
MeSH headings : Electrophoresis, Capillary; Humans; K562 Cells; Leukemia / enzymology; Phosphotransferases (Alcohol Group Acceptor) / metabolism
Source: Web Of Science
Added: August 6, 2018

2014 journal article

Small Sample Sorting of Primary Adherent Cells by Automated Micropallet Imaging and Release

CYTOMETRY PART A, 85A(7), 642–649.

By: P. Shah n, S. Herrera-Loeza*, C. Sims*, J. Yeh* & N. Allbritton n

author keywords: image cytometry; xenografts; cell sorting; micropallet arrays
MeSH headings : Animals; Antigens, Neoplasm / analysis; Cell Adhesion Molecules / analysis; Colorectal Neoplasms / pathology; Electronic Data Processing; Epithelial Cell Adhesion Molecule; Flow Cytometry / methods; Heterografts / cytology; Humans; Image Processing, Computer-Assisted; Mice; Mice, Nude; Pancreatic Neoplasms / pathology; Staining and Labeling; Tumor Cells, Cultured
Source: Web Of Science
Added: August 6, 2018

2013 journal article

A comparative analysis of the ubiquitination kinetics of multiple degrons to identify an ideal targeting sequence for a proteasome reporter

PLoS One, 8(10).

By: A. Melvin, G. Woss, J. Park, L. Dumberger, M. Waters & N. Allbritton

Source: NC State University Libraries
Added: August 6, 2018

2013 journal article

Array of biodegradable microrafts for isolation and implantation of living, adherent cells

RSC Advances, 3(24), 9264–9272.

By: Y. Wang, C. Phillips, G. Herrera, C. Sims, J. Yeh & N. Allbritton

Source: NC State University Libraries
Added: August 6, 2018

2013 journal article

Automated Capillary Electrophoresis System for Fast Single-Cell Analysis

ANALYTICAL CHEMISTRY, 85(9), 4797–4804.

By: A. Dickinson*, P. Armistead* & N. Allbritton n

MeSH headings : Animals; Automation; Electrophoresis, Capillary; PC12 Cells; Rats; Single-Cell Analysis; Tumor Cells, Cultured
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Capture and 3D culture of colonic crypts and colonoids in a microarray platform

Lab on a Chip, 13(23), 4625–4634.

By: Y. Wang, A. Ahmad, P. Shah, C. Sims, S. Magness & N. Allbritton

Source: NC State University Libraries
Added: August 6, 2018

2013 journal article

Characterization of freestanding photoresist films for biological and MEMS applications

Journal of Micromechanics and Microengineering, 23(2).

By: D. Ornoff, Y. Wang & N. Allbritton

Source: NC State University Libraries
Added: August 6, 2018

2013 journal article

Efficient division and sampling of cell colonies using microcup arrays

ANALYST, 138(1), 220–228.

By: J. Pai*, K. Kluckman*, D. Cowley*, D. Bortner*, C. Sims* & N. Allbritton*

MeSH headings : Animals; Cell Culture Techniques; Cell Separation / methods; Embryonic Stem Cells / cytology; Lasers; Mice; Microtechnology; Single-Cell Analysis; Time Factors; Tissue Array Analysis / methods
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Fabrication of 3D Microstructures from Interactions of Immiscible Liquids with a Structured Surface

ADVANCED MATERIALS, 25(30), 4107–4112.

By: J. Balowski*, Y. Wang* & N. Allbritton n

MeSH headings : Colloids / chemistry; Dimethylpolysiloxanes / chemistry; Macromolecular Substances / chemistry; Materials Testing; Microfluidics / instrumentation; Microfluidics / methods; Molecular Conformation; Molecular Imprinting / methods; Solutions / chemistry; Surface Properties
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Isolation and in Vitro Culture of Rare Cancer Stem Cells from Patient-Derived Xenografts of Pancreatic Ductal Adenocarcinoma

ANALYTICAL CHEMISTRY, 85(15), 7271–7278.

By: P. Gach*, P. Attayek n, G. Herrera*, J. Yeh* & N. Allbritton n

MeSH headings : Animals; Carcinoma, Pancreatic Ductal / pathology; Cell Culture Techniques / methods; Cell Line, Tumor; Cell Separation / methods; Cell Survival; Cell Transformation, Neoplastic; Humans; Image Processing, Computer-Assisted; Mice; Neoplastic Stem Cells / pathology; Pancreatic Neoplasms / pathology
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Laser-based directed release of array elements for efficient collection into targeted microwells

ANALYST, 138(3), 831–838.

By: N. Dobes*, R. Dhopeshwarkar*, W. Henley*, J. Ramsey n, C. Sims* & N. Allbritton n

MeSH headings : Cell Line, Tumor; Cell Separation; Cell Survival; Dimethylpolysiloxanes / chemistry; Green Fluorescent Proteins / genetics; Green Fluorescent Proteins / metabolism; HeLa Cells; Humans; Lasers; Tissue Array Analysis; Transfection
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Measurement of Protein Tyrosine Phosphatase Activity in Single Cells by Capillary Electrophoresis

ANALYTICAL CHEMISTRY, 85(12), 6136–6142.

By: R. Phillips*, E. Bair*, D. Lawrence*, C. Sims* & N. Allbritton n

MeSH headings : Cell Line, Tumor; Cell Separation / methods; Electrophoresis, Capillary / methods; Enzyme Activation / physiology; Flow Cytometry / methods; Humans; Protein Tyrosine Phosphatases / analysis; Protein Tyrosine Phosphatases / metabolism
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Measuring Activity in the Ubiquitin-Proteasome System: From Large Scale Discoveries to Single Cells Analysis

CELL BIOCHEMISTRY AND BIOPHYSICS, 67(1), 75–89.

By: A. Melvin*, G. Woss*, J. Park*, M. Waters* & N. Allbritton*

author keywords: Ubiquitin; Proteasome; E3 Ligase; DUB; Reporter
Source: Web Of Science
Added: August 6, 2018

2013 review

Micro Total Analysis Systems: Fundamental Advances and Applications in the Laboratory, Clinic, and Field

[Review of ]. ANALYTICAL CHEMISTRY, 85(2), 451–472.

By: M. Kovarik*, D. Ornoff*, A. Melvin*, N. Dobes*, Y. Wang*, A. Dickinson*, P. Gach*, P. Shah n, N. Allbritton n

MeSH headings : Clinical Laboratory Techniques / instrumentation; Equipment Design; Laboratories; Microtechnology / instrumentation
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Microfluidic Chemical Cytometry of Peptide Degradation in Single Drug-Treated Acute Myeloid Leukemia Cells

ANALYTICAL CHEMISTRY, 85(10), 4991–4997.

By: M. Kovarik*, P. Shah n, P. Armistead* & N. Allbritton n

MeSH headings : Amino Acid Sequence; Antineoplastic Agents / pharmacology; Cell Line, Tumor; Humans; Kinetics; Leukemia, Myeloid, Acute / pathology; Microfluidic Analytical Techniques / methods; Peptides / chemistry; Peptides / metabolism; Proteolysis / drug effects
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Scalable synthesis of a biocompatible, transparent and superparamagnetic photoresist for microdevice fabrication

Journal of Micromechanics and Microengineering, 23(10).

By: P. Shah, M. Hughes, Y. Wang, C. Sims & N. Allbritton

Source: NC State University Libraries
Added: August 6, 2018

2013 journal article

beta-Turn sequences promote stability of peptide substrates for kinases within the cytosolic environment

Analyst [London], 138(15), 4305–4311.

By: S. Yang, A. Proctor, L. Cline, K. Houston, M. Waters & N. Allbritton

Source: NC State University Libraries
Added: August 6, 2018

2012 journal article

Development of a Peptidase-Resistant Substrate for Single-Cell Measurement of Protein Kinase B Activation

ANALYTICAL CHEMISTRY, 84(16), 7195–7202.

By: A. Proctor*, Q. Wang*, D. Lawrence* & N. Allbritton n

MeSH headings : Amino Acid Sequence; Amino Acid Substitution; Arginine; Cytosol / metabolism; Drug Design; Enzyme Activation / drug effects; HeLa Cells; Humans; Oligopeptides / chemistry; Oligopeptides / metabolism; Oligopeptides / pharmacology; Peptide Hydrolases / metabolism; Phosphorylation; Protein Binding; Protein Kinase Inhibitors / chemistry; Protein Kinase Inhibitors / metabolism; Protein Kinase Inhibitors / pharmacology; Proteolysis; Proto-Oncogene Proteins c-akt / antagonists & inhibitors; Proto-Oncogene Proteins c-akt / metabolism; Single-Cell Analysis
Source: Web Of Science
Added: August 6, 2018

2012 journal article

Dissolution-guided wetting for microarray and microfluidic devices

Lab on a Chip, 12(17), 3036–3039.

By: Y. Wang, C. Sims & N. Allbritton

Source: NC State University Libraries
Added: August 6, 2018

2012 journal article

Metabolism of peptide reporters in cell lysates and single cells

Analyst [London], 137(13), 3028–3038.

By: A. Proctor, Q. Wang, D. Lawrence & N. Allbritton

Source: NC State University Libraries
Added: August 6, 2018

2012 review

Micro Total Analysis Systems for Cell Biology and Biochemical Assays

[Review of ]. ANALYTICAL CHEMISTRY, 84(2), 516–540.

By: M. Kovarik*, P. Gach*, D. Ornoff*, Y. Wang*, J. Balowski*, L. Farrag*, N. Allbritton n

MeSH headings : Animals; Biological Assay; Clinical Laboratory Techniques / instrumentation; Clinical Laboratory Techniques / trends; Humans; Microfluidic Analytical Techniques
Source: Web Of Science
Added: August 6, 2018

2012 journal article

Microfabricated Arrays for Splitting and Assay of Clonal Colonies

ANALYTICAL CHEMISTRY, 84(24), 10614–10620.

By: P. Gach, W. Xu, S. King, C. Sims, J. Bear & N. Allbritton n

MeSH headings : Animals; Cell Separation / methods; Cells, Cultured; Clone Cells / cytology; Cloning, Molecular / methods; HeLa Cells; Humans; Mice; Microtechnology / methods; NIH 3T3 Cells
Source: Web Of Science
Added: August 6, 2018

2012 journal article

Polystyrene-coated micropallets for culture and separation of primary muscle cells

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 402(3), 1083–1091.

By: D. Detwiler*, N. Dobes*, C. Sims*, J. Kornegay* & N. Allbritton n

author keywords: Bioanalytical methods; Biomaterials; Cell systems; Single cell analysis
MeSH headings : Animals; Cell Adhesion; Cell Culture Techniques / instrumentation; Cell Proliferation; Cell Separation / instrumentation; Cells, Cultured; Dogs; Muscle Cells / cytology; Polystyrenes / chemistry; Surface Properties; Tissue Array Analysis / instrumentation
Source: Web Of Science
Added: August 6, 2018

2012 journal article

Separation of fluorescently labeled phosphoinositides and sphingolipids by capillary electrophoresis

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 907, 79–86.

By: K. Wang*, D. Jiang*, C. Sims* & N. Allbritton n

author keywords: Capillary electrophoresis; Sphingolipid; Phosphoinositide lipid; Phospholipid separation
MeSH headings : 1-Propanol / chemistry; Boron Compounds / chemistry; Cell Line, Tumor; Electrophoresis, Capillary / methods; Fluorescein / chemistry; Fluorescent Dyes / chemistry; Humans; Hydrogen-Ion Concentration; Limit of Detection; Phosphates / chemistry; Phosphatidylinositols / chemistry; Phosphatidylinositols / isolation & purification; Reproducibility of Results; Sphingolipids / chemistry; Sphingolipids / isolation & purification
Source: Web Of Science
Added: August 6, 2018

2012 journal article

Trapping cells on a stretchable microwell array for single-cell analysis

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 402(3), 1065–1072.

By: Y. Wang*, P. Shah n, C. Phillips*, C. Sims* & N. Allbritton n

author keywords: Bioanalytical methods; Cell systems/single-cell analysis; Biochips/high-throughput screening
MeSH headings : Animals; Calcium / metabolism; Cell Line; Dimethylpolysiloxanes / chemistry; Equipment Design; Ionomycin / metabolism; Single-Cell Analysis / economics; Single-Cell Analysis / instrumentation; Tissue Array Analysis / economics; Tissue Array Analysis / instrumentation
Source: Web Of Science
Added: August 6, 2018

2012 article

Trapping cells on a stretchable microwell array for single-cell analysis (vol 402, pg 1065, 2012)

Analytical and Bioanalytical Chemistry, Vol. 404, pp. 2519–2519.

By: Y. Wang, P. Shah, C. Phillips, C. Sims & N. Allbritton

Source: NC State University Libraries
Added: August 6, 2018

2011 journal article

Benchtop micromolding of polystyrene by soft lithography

Lab on a Chip, 11(18), 3089–3097.

By: Y. Wang, J. Balowski, C. Phillips, R. Phillips, C. Sims & N. Allbritton

Source: NC State University Libraries
Added: August 6, 2018

2011 journal article

Continuous analysis of dye-loaded, single cells on a microfluidic chip

Lab on a Chip, 11(7), 1333–1341.

By: K. Phillips, H. Lai, E. Johnson, C. Sims & N. Allbritton

Source: NC State University Libraries
Added: August 6, 2018

2011 journal article

Femtomole SHAPE Reveals Regulatory Structures in the Authentic XMRV RNA Genome

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 133(50), 20326–20334.

By: J. Grohman, S. Kottegoda, R. Gorelick*, N. Allbritton* & K. Weeks

MeSH headings : Acetylation; Base Sequence; Electrophoresis, Capillary; Genome, Viral; Leukemia Virus, Murine / genetics; Molecular Sequence Data; Nucleic Acid Conformation; RNA, Viral / chemistry; RNA, Viral / genetics
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Isolation and manipulation of living adherent cells by micromolded magnetic rafts

Biomicrofluidics, 5(3).

By: P. Gach, Y. Wang, C. Phillips, C. Sims & N. Allbritton

Source: NC State University Libraries
Added: August 6, 2018

2011 journal article

Kinetic analysis of PI3K reactions with fluorescent PIP2 derivatives

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 401(6), 1881–1888.

By: W. Huang*, D. Jiang*, X. Wang*, K. Wang*, C. Sims*, N. Allbritton*, Q. Zhang*

author keywords: PI3K assay; Fluorescent phosphatidylinositides; TLC analysis; Capillary electrophoresis
MeSH headings : Chromatography, Thin Layer / methods; Electrophoresis, Capillary / methods; Enzyme Inhibitors / pharmacology; Fluorescent Dyes / chemistry; Fluorescent Dyes / metabolism; Kinetics; Phosphatidylinositol 3-Kinase / metabolism; Phosphatidylinositol 4,5-Diphosphate / chemistry; Phosphatidylinositol 4,5-Diphosphate / metabolism; Phosphoinositide-3 Kinase Inhibitors; Sensitivity and Specificity
Source: Web Of Science
Added: August 6, 2018

2011 review

Measuring enzyme activity in single cells

[Review of ]. TRENDS IN BIOTECHNOLOGY, 29(5), 222–230.

By: M. Kovarik* & N. Allbritton n

MeSH headings : Cells / metabolism; Cytological Techniques / methods; Electrophoresis, Capillary / methods; Enzymes / metabolism; Flow Cytometry / methods; Microscopy / methods
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Patterning pallet arrays for cell selection based on high-resolution measurements of fluorescent biosensors

ANALYTICA CHIMICA ACTA, 696(1-2), 101–107.

By: H. Shadpour, J. Zawistowski, A. Herman, K. Hahn & N. Allbritton*

author keywords: Pallet array; Cell separation; Patterned surfaces; FRET; Cell sorting; Micro-contact printing
MeSH headings : Animals; Biosensing Techniques / methods; Cell Line; Cell Separation / methods; Fibronectins / chemistry; Fluorescence Resonance Energy Transfer / methods; HeLa Cells; Humans; Mice; Tissue Array Analysis / methods; rhoA GTP-Binding Protein / metabolism
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Sample transport and electrokinetic injection in a microchip device for chemical cytometry

ELECTROPHORESIS, 32(22), 3180–3187.

By: M. Kovarik*, H. Lai*, J. Xiong* & N. Allbritton n

author keywords: Chemical cytometry; Electrokinetic injection bias; Microfluidics; Simulation
MeSH headings : Computer Simulation; Equipment Design; Microfluidic Analytical Techniques / instrumentation; Models, Chemical; Peptides / chemistry; Phosphatidylcholines / chemistry; Phosphatidylglycerols / chemistry; Pressure; Reproducibility of Results; Single-Cell Analysis / instrumentation; Single-Cell Analysis / methods
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Selection and Separation of Viable Cells Based on a Cell-Lethal Assay

ANALYTICAL CHEMISTRY, 83(1), 278–283.

By: W. Xu n, A. Herman n, C. Phillips n, J. Pai n, C. Sims n & N. Allbritton n

MeSH headings : Animals; Cell Adhesion; Cell Culture Techniques; Cell Separation / instrumentation; Cell Separation / methods; Cell Survival; Enzyme Activation; Extracellular Signal-Regulated MAP Kinases / metabolism; Feasibility Studies; HeLa Cells; Humans; Mice; NIH 3T3 Cells; Tissue Array Analysis
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Single-cell analysis of phosphoinositide 3-kinase and phosphatase and tensin homolog activation

Faraday Discussions, 149, 187–200.

By: D. Jiang, C. Sims & N. Allbritton

Source: NC State University Libraries
Added: August 6, 2018

2011 journal article

Use of a virtual wall valve in polydimethylsiloxane microfluidic devices for bioanalytical applications

Biomicrofluidics, 5(2).

By: H. Lai, W. Xu & N. Allbritton

Source: NC State University Libraries
Added: August 6, 2018

2010 journal article

Air-stable supported membranes for single-cell cytometry on PDMS microchips

Lab on a Chip, 10(7), 864–870.

By: K. Phillips, K. Kang, L. Licata & N. Allbritton

Source: NC State University Libraries
Added: August 6, 2018

2010 journal article

Contact printing of arrayed microstructures

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 397(8), 3377–3385.

By: W. Xu*, A. Luikart*, C. Sims* & N. Allbritton n

author keywords: Contact printing; Microfabrication; SU-8; Cell culture; Micropallet
MeSH headings : Acrylic Resins / chemistry; Animals; Biocompatible Materials / chemistry; Cell Adhesion; Cell Culture Techniques; Cell Line; Extracellular Matrix / chemistry; Humans; Mice; Polystyrenes / chemistry; Surface Properties; Tissue Array Analysis / instrumentation
Source: Web Of Science
Added: August 6, 2018

2010 journal article

In situ Roughening of Polymeric Microstructures

ACS APPLIED MATERIALS & INTERFACES, 2(4), 1086–1093.

By: H. Shadpour n & N. Allbritton n

author keywords: roughening; in situ; polymer; MEMS; microarray; printing; cell
MeSH headings : Aluminum Oxide / chemistry; Animals; Biocompatible Materials / chemistry; Cattle; Cell Adhesion; Cell Culture Techniques / methods; Cell Proliferation; Cyclic AMP / analogs & derivatives; Cyclic AMP / chemistry; Fibronectins / chemistry; Fluorescent Dyes / chemistry; Microscopy, Atomic Force / methods; Polymers / chemistry; Polystyrenes / chemistry; Serum Albumin / chemistry; Surface Properties
Source: Web Of Science
Added: August 6, 2018

2010 journal article

Microcup Arrays for the Efficient Isolation and Cloning of Cells

ANALYTICAL CHEMISTRY, 82(8), 3161–3167.

By: W. Xu n, C. Sims n & N. Allbritton n

MeSH headings : 3T3 Cells; Animals; Cell Separation / instrumentation; Cell Separation / methods; Clone Cells; HeLa Cells; Hematopoietic Stem Cells / cytology; Humans; Magnetics; Mice; Tissue Array Analysis
Source: Web Of Science
Added: August 6, 2018

2010 journal article

Microdevice to capture colon crypts for in vitro studies

Lab on a Chip, 10(12), 1596–1603.

By: Y. Wang, R. Dhopeshwarkar, R. Najdi, M. Waterman, C. Sims & N. Allbritton

Source: NC State University Libraries
Added: August 6, 2018

2010 journal article

Microelectrophoresis platform for fast serial analysis of single cells

Electrophoresis, 31(15), 2558–2565.

By: D. Jiang, C. Sims & N. Allbritton

Source: NC State University Libraries
Added: August 6, 2018

2010 journal article

Micromolded arrays for separation of adherent cells

Lab on a Chip, 10(21), 2917–2924.

By: Y. Wang, C. Phillips, W. Xu, J. Pai, R. Dhopeshwarkar, C. Sims, N. Allbritton

Source: NC State University Libraries
Added: August 6, 2018

2010 journal article

Microtable arrays for culture and isolation of cell colonies

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 398(6), 2595–2604.

By: J. Pai*, W. Xu*, C. Sims* & N. Allbritton*

author keywords: Microtable; Micropallet; Microfabrication; 1002F; SU-8; Cell separation; Cell array
MeSH headings : Cell Culture Techniques / instrumentation; Cell Culture Techniques / methods; Cell Separation / instrumentation; Cell Separation / methods; Culture Media; Equipment Design; HeLa Cells; Humans; Microarray Analysis / instrumentation; Microarray Analysis / methods; Microscopy, Electron, Scanning; Microscopy, Fluorescence; Surface Properties
Source: Web Of Science
Added: August 6, 2018

2010 journal article

Transparent magnetic photoresists for bioanalytical applications

BIOMATERIALS, 31(33), 8810–8817.

By: P. Gach*, C. Sims* & N. Allbritton n

author keywords: Magnetism; Photolithography; Biocomaptibility; Micropatterning; MTT assay; Cell adhesion
MeSH headings : Absorption; Animals; Biomedical Technology / instrumentation; Cell Line; Cell Proliferation; Cell Separation; Colorimetry; Ferric Compounds / chemistry; Humans; Magnetics / instrumentation; Nanoparticles / ultrastructure; Photochemistry / instrumentation
Source: Web Of Science
Added: August 6, 2018

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