Nancy Lynn Allbritton

Hyperglycemia minimally alters primary self-renewing human colonic epithelial cells while TNFα-promotes severe intestinal epithelial dysfunction

Dutton, J. S., Hinman, S. S., Kim, R., Attayek, P. J., Maurer, M., Sims, C. S., & Allbritton, N. L. (2021, May 14). Integrative Biology.

Magnetically-propelled fecal surrogates for modeling the impact of solid-induced shear forces on primary colonic epithelial cells

Hinman, S. S., Huling, J., Wang, Y., Wang, H., Bretherton, R. C., DeForest, C. A., & Allbritton, N. L. (2021, August 12). Biomaterials.

Stem/Proliferative and Differentiated Cells within Primary Murine Colonic Epithelium Display Distinct Intracellular Free Ca²⁺ Signal Codes

Mestril, S., Kim, R., Hinman, S. S., Gomez, S. M., & Allbritton, N. L. (2021, September 12). Advanced Healthcare Materials.

Automated platform for cell selection and separation based on four-dimensional motility and matrix degradation

Nowotarski, H. L., Attayek, P. J., & Allbritton, N. L. (2020, January 1). The Analyst.

Image-Based Live Cell Sorting

LaBelle, C. A., Massaro, A., Cortés-Llanos, B., Sims, C. E., & Allbritton, N. L. (2020, November 12). Trends in Biotechnology.

Microraft array-based platform for sorting of viable microcolonies based on cell-lethal immunoassay of intracellular proteins in microcolony biopsies

Smiddy, N. M., DiSalvo, M., Allbritton-King, J. D., & Allbritton, N. L. (2020, January 1). The Analyst.

Pooled CRISPR screens with imaging on microraft arrays reveals stress granule-regulatory factors

Wheeler, E. C., Vu, A. Q., Einstein, J. M., DiSalvo, M., Ahmed, N., Nostrand, E. L. V., … Yeo, G. W. (2020, May 11). Nature Methods.

An in vitro intestinal platform with a self-sustaining oxygen gradient to study the human gut/microbiome interface

Kim, R., Attayek, P. J., Wang, Y., Furtado, K. L., Tamayo, R., Sims, C. E., & Allbritton, N. L. (2019, September 13). Biofabrication.

Assay and Isolation of Single Proliferating CD4+ Lymphocytes Using an Automated Microraft Array Platform

LaBelle, C. A., Zhang, R. J., Armistead, P. M., & Allbritton, N. L. (2019, November 26). IEEE Transactions on Biomedical Engineering.

Automated sensing and splitting of stem cell colonies on microraft arrays

DiSalvo, M., Smiddy, N. M., & Allbritton, N. L. (2019, August 29). APL Bioengineering.

Building a Thick Mucus Hydrogel Layer to Improve the Physiological Relevance of In Vitro Primary Colonic Epithelial Models

Wang, Y., Kim, R., Sims, C. E., & Allbritton, N. L. (2019, January 1). Cellular and Molecular Gastroenterology and Hepatology.

Evaluation of human primary intestinal monolayers for drug metabolizing capabilities

Speer, J. E., Wang, Y., Fallon, J. K., Smith, P. C., & Allbritton, N. L. (2019, November 4). Journal of Biological Engineering.

Molecular transport through primary human small intestinal monolayers by culture on a collagen scaffold with a gradient of chemical cross-linking

Speer, J. E., Gunasekara, D. B., Wang, Y., Fallon, J. K., Attayek, P. J., Smith, P. C., … Allbritton, N. L. (2019, April 27). Journal of Biological Engineering.

Photopatterned Membranes and Chemical Gradients Enable Scalable Phenotypic Organization of Primary Human Colon Epithelial Models

Hinman, S. S., Wang, Y., & Allbritton, N. L. (2019, November 6). Analytical Chemistry.

Preserving single cells in space and time for analytical assays

Gallion, L. A., Anttila, M. M., Abraham, D. H., Proctor, A., & Allbritton, N. L. (2019, November 7). TrAC Trends in Analytical Chemistry.

Selection and optimization of enzyme reporters for chemical cytometry

Proctor, A., Wang, Q., Lawrence, D. S., & Allbritton, N. L. (2019, January 1). Methods in Enzymology on CD-ROM/Methods in Enzymology.

A Monolayer of Primary Colonic Epithelium Generated on a Scaffold with a Gradient of Stiffness for Drug Transport Studies

Gunasekara, D. B., Speer, J., Wang, Y., Nguyen, D. L., Reed, M. I., Smiddy, N. M., … Allbritton, N. L. (2018, October 16). Analytical Chemistry.

Analysis of Interleukin 8 Secretion by a Stem-Cell-Derived Human-Intestinal-Epithelial-Monolayer Platform

Wang, Y., Kim, R., Hwang, S.-H. J., Dutton, J., Sims, C. E., & Allbritton, N. L. (2018, September 10). Analytical Chemistry.

Bioengineered systems and designer matrices that recapitulate the intestinal stem cell niche

[Review of ]. Cellular and Molecular Gastroenterology and Hepatology, 5(3), 440-.

Characterization of Tensioned PDMS Membranes for Imaging Cytometry on Microraft Arrays

DiSalvo, M., Harris, D. M., Kantesaria, S., Peña, A. N., Allbritton-King, J. D., Cole, J. H., & Allbritton, N. L. (2018, March 6). Analytical Chemistry, Vol. 90, pp. 4792–4800.

LITE microscopy: Tilted light-sheet excitation of model organisms offers high resolution and low photobleaching

Fadero, T. C., Gerbich, T. M., Rana, K., Suzuki, A., DiSalvo, M., Schaefer, K. N., … Maddox, P. S. (2018, February 28). The Journal of Cell Biology.

Primary Cell-Derived Intestinal Models: Recapitulating Physiology

Dutton, J. S., Hinman, S. S., Kim, R., Wang, Y., & Allbritton, N. L. (2018, December 24). Trends in Biotechnology.

“Fix and assay”: separating in-cellulo sphingolipid reactions from analytical assay in time and space using an aldehyde-based fixative

Proctor, A., & Allbritton, N. L. (2018, September 7). The Analyst.

A microengineered collagen scaffold for generating a polarized crypt-villus architecture of human small intestinal epithelium

Wang, Y., Gunasekara, D. B., Reed, M. I., DiSalvo, M., Bultman, S. J., Sims, C. E., … Allbritton, N. L. (2017, March 6). Biomaterials.

Chemical fixation to arrest phospholipid signaling for chemical cytometry

Proctor, A., Sims, C. E., & Allbritton, N. L. (2017, May 10). Journal of Chromatography A.

Formation of Human Colonic Crypt Array by Application of Chemical Gradients Across a Shaped Epithelial Monolayer

Wang, Y., Kim, R., Gunasekara, D. B., Reed, M. I., DiSalvo, M., Nguyen, D. L., … Allbritton, N. L. (2017, November 3). Cellular and Molecular Gastroenterology and Hepatology.

N-Gemini peptides: cytosolic protease resistance via N-terminal dimerization of unstructured peptides

Fayer, E. L., Gilliland, W. M., Ramsey, J. M., Allbritton, N. L., & Waters, M. L. (2017, December 12). Chemical Communications.

Nonsteroidal Anti-Inflammatory Drug-Induced Leaky Gut Modeled Using Polarized Monolayers of Primary Human Intestinal Epithelial Cells

Bhatt, A. P., Gunasekara, D. B., Speer, J., Reed, M. I., Peña, A. N., Midkiff, B. R., … Redinbo, M. R. (2017, November 2). ACS Infectious Diseases.

Required hydrophobicity of fluorescent reporters for phosphatidylinositol family of lipid enzymes

Waybright, J., Huang, W., Proctor, A., Wang, X., Allbritton, N. L., & Zhang, Q. (2017, September 20). Analytical and Bioanalytical Chemistry.

Self-renewing monolayer of primary colonic or rectal epithelial cells

Cellular and Molecular Gastroenterology and Hepatology, 4(1), 165-.

An Integrated Chemical Cytometry Method: Shining a Light on Akt Activity in Single Cells

Mainz, E. R., Wang, Q., Lawrence, D. S., & Allbritton, N. L. (2016, September 20). Angewandte Chemie International Edition.

Automated microraft platform to identify and collect non-adherent cells successfully gene-edited with CRISPR-Cas9

Attayek, P. J., Waugh, J. P., Hunsucker, S. A., Grayeski, P. J., Sims, C. E., Armistead, P. M., & Allbritton, N. L. (2016, December 10). Biosensors and Bioelectronics.

Development of a protease-resistant reporter to quantify BCR–ABL activity in intact cells

Proctor, A., Zigoneanu, I. G., Wang, Q., Sims, C. E., Lawrence, D. S., & Allbritton, N. L. (2016, January 1). The Analyst.

Identification and isolation of antigen-specific cytotoxic T lymphocytes with an automated microraft sorting system

Attayek, P. J., Hunsucker, S. A., Sims, C. E., Allbritton, N. L., & Armistead, P. M. (2016, January 1). Integrative Biology.

In vitro polarization of colonoids to create an intestinal stem cell compartment

PLoS One, 11(4).

Single Cell Chemical Cytometry of Akt Activity in Rheumatoid Arthritis and Normal Fibroblast-like Synoviocytes in Response to Tumor Necrosis Factor α

Mainz, E. R., Serafin, D. S., Nguyen, T. T., Tarrant, T. K., Sims, C. E., & Allbritton, N. L. (2016, July 8). Analytical Chemistry.

Analysis of sphingosine kinase activity in single natural killer cells from peripheral blood

Dickinson, A. J., Meyer, M., Pawlak, E. A., Gomez, S., Jaspers, I., & Allbritton, N. L. (2015, January 1). Integrative Biology.

Array-Based Platform To Select, Release, and Capture Epstein–Barr Virus-Infected Cells Based on Intercellular Adhesion

Attayek, P. J., Hunsucker, S. A., Wang, Y., Sims, C. E., Armistead, P. M., & Allbritton, N. L. (2015, November 11). Analytical Chemistry.

Co-fabrication of chitosan and epoxy photoresist to form microwell arrays with permeable hydrogel bottoms

Ornoff, D. M., Wang, Y., Proctor, A., Shah, A. S., & Allbritton, N. L. (2015, September 29). Biomaterials.

Magnetic Alignment of Microelements Containing Cultured Neuronal Networks for High-Throughput Screening

Gordon, K. R., Wang, Y., Allbritton, N. L., & Taylor, A. M. (2015, August 6). SLAS DISCOVERY.

Optimizing Wnt-3a and R-spondin1 concentrations for stem cell renewal and differentiation in intestinal organoids using a gradient-forming microdevice

Ahmad, A. A., Wang, Y., Sims, C. E., Magness, S. T., & Allbritton, N. L. (2015, January 1). RSC Advances.

Pronase E-Based Generation of Fluorescent Peptide Fragments: Tracking Intracellular Peptide Fate in Single Cells

Mainz, E. R., Dobes, N. C., & Allbritton, N. L. (2015, July 14). Analytical Chemistry.

Rational Design of a Dephosphorylation-Resistant Reporter Enables Single-Cell Measurement of Tyrosine Kinase Activity

Turner, A. H., Lebhar, M. S., Proctor, A., Wang, Q., Lawrence, D. S., & Allbritton, N. L. (2015, November 20). ACS Chemical Biology.

Separation of peptide fragments of a protein kinase C substrate fused to a β-hairpin by capillary electrophoresis

Zigoneanu, I. G., Sims, C. E., & Allbritton, N. L. (2015, October 1). Analytical and Bioanalytical Chemistry.

Single‐cell functional analysis of parathyroid adenomas reveals distinct classes of calcium sensing behaviour in primary hyperparathyroidism

Koh, J., Hogue, J. A., Wang, Y., DiSalvo, M., Allbritton, N. L., Shi, Y., … Sosa, J. A. (2015, December 5). Journal of Cellular and Molecular Medicine.

An Immature Retroviral RNA Genome Resembles a Kinetically Trapped Intermediate State

Grohman, J. K., Gorelick, R. J., Kottegoda, S., Allbritton, N. L., Rein, A., & Weeks, K. M. (2014, March 13). Journal of Virology.

Dynamics and evolution of β-catenin-dependent Wnt signaling revealed through massively parallel clonogenic screening

Shah, P. K., Walker, M. P., Sims, C. E., Major, M. B., & Allbritton, N. L. (2014, January 1). Integrative Biology.

Measurement of Protein Kinase B Activity in Single Primary Human Pancreatic Cancer Cells

Proctor, A., Herrera-Loeza, S. G., Wang, Q., Lawrence, D. S., Yeh, J. J., & Allbritton, N. L. (2014, April 9). Analytical Chemistry.

Response of single leukemic cells to peptidase inhibitor therapy across time and dose using a microfluidic device

Kovarik, M. L., Dickinson, A. J., Roy, P., Poonnen, R. A., Fine, J. P., & Allbritton, N. L. (2014, January 1). Integrative Biology.

Single-cell sphingosine kinase activity measurements in primary leukemia

Dickinson, A. J., Hunsucker, S. A., Armistead, P. M., & Allbritton, N. L. (2014, June 30). Analytical and Bioanalytical Chemistry.

Small sample sorting of primary adherent cells by automated micropallet imaging and release

Shah, P. K., Herrera‐Loeza, S. G., Sims, C. E., Yeh, J. J., & Allbritton, N. L. (2014, June 17). Cytometry Part A.

A comparative analysis of the ubiquitination kinetics of multiple degrons to identify an ideal targeting sequence for a proteasome reporter

PLoS One, 8(10).

Array of biodegradable microrafts for isolation and implantation of living, adherent cells

RSC Advances, 3(24), 9264–9272.

Automated Capillary Electrophoresis System for Fast Single-Cell Analysis

Dickinson, A. J., Armistead, P. M., & Allbritton, N. L. (2013, March 25). Analytical Chemistry.

Capture and 3D culture of colonic crypts and colonoids in a microarray platform

Lab on a Chip, 13(23), 4625–4634.

Characterization of freestanding photoresist films for biological and MEMS applications

Journal of Micromechanics and Microengineering, 23(2).

Ex Vivo Chemical Cytometric Analysis of Protein Tyrosine Phosphatase Activity in Single Human Airway Epithelial Cells

Phillips, R. M., Dailey, L. A., Bair, E., Samet, J. M., & Allbritton, N. L. (2013, December 24). Analytical Chemistry.

Fabrication of 3D Microstructures from Interactions of Immiscible Liquids with a Structured Surface

Balowski, J. J., Wang, Y., & Allbritton, N. L. (2013, June 25). Advanced Materials.

Isolation and in Vitro Culture of Rare Cancer Stem Cells from Patient-Derived Xenografts of Pancreatic Ductal Adenocarcinoma

Gach, P. C., Attayek, P. J., Herrera, G., Yeh, J. J., & Allbritton, N. L. (2013, July 1). Analytical Chemistry.

Measurement of Protein Tyrosine Phosphatase Activity in Single Cells by Capillary Electrophoresis

Phillips, R. M., Bair, E., Lawrence, D. S., Sims, C. E., & Allbritton, N. L. (2013, May 20). Analytical Chemistry.

Measuring Activity in the Ubiquitin–Proteasome System: From Large Scale Discoveries to Single Cells Analysis

Melvin, A. T., Woss, G. S., Park, J. H., Waters, M. L., & Allbritton, N. L. (2013, May 17). Cell Biochemistry and Biophysics.

Microfluidic Chemical Cytometry of Peptide Degradation in Single Drug-Treated Acute Myeloid Leukemia Cells

Kovarik, M. L., Shah, P. K., Armistead, P. M., & Allbritton, N. L. (2013, April 17). Analytical Chemistry.

Micropallet arrays for the capture, isolation and culture of circulating tumor cells from whole blood of mice engrafted with primary human pancreatic adenocarcinoma

Gach, P. C., Attayek, P. J., Whittlesey, R. L., Yeh, J. J., & Allbritton, N. L. (2013, November 20). Biosensors and Bioelectronics.

Scalable synthesis of a biocompatible, transparent and superparamagnetic photoresist for microdevice fabrication

Journal of Micromechanics and Microengineering, 23(10).

beta-Turn sequences promote stability of peptide substrates for kinases within the cytosolic environment

Analyst [London], 138(15), 4305–4311.

Development of a Peptidase-Resistant Substrate for Single-Cell Measurement of Protein Kinase B Activation

Proctor, A., Wang, Q., Lawrence, D. S., & Allbritton, N. L. (2012, July 31). Analytical Chemistry.

Dissolution-guided wetting for microarray and microfluidic devices

Lab on a Chip, 12(17), 3036–3039.

Efficient division and sampling of cell colonies using microcup arrays

Pai, J.-H., Kluckman, K., Cowley, D. O., Bortner, D. M., Sims, C. E., & Allbritton, N. L. (2012, October 19). The Analyst.

Laser-based directed release of array elements for efficient collection into targeted microwells

Dobes, N. C., Dhopeshwarkar, R., Henley, W. H., Ramsey, J. M., Sims, C. E., & Allbritton, N. L. (2012, November 28). The Analyst.

Metabolism of peptide reporters in cell lysates and single cells

Analyst [London], 137(13), 3028–3038.

Micro Total Analysis Systems: Fundamental Advances and Applications in the Laboratory, Clinic, and Field

Kovarik, M. L., Ornoff, D. M., Melvin, A. T., Dobes, N. C., Wang, Y., Dickinson, A. J., … Allbritton, N. L. (2012, November 9). Analytical Chemistry.

Microfabricated Arrays for Splitting and Assay of Clonal Colonies

Gach, P. C., Xu, W., King, S. J., Sims, C. E., Bear, J., & Allbritton, N. L. (2012, November 16). Analytical Chemistry.

Separation of fluorescently labeled phosphoinositides and sphingolipids by capillary electrophoresis

Wang, K., Jiang, D., Sims, C. E., & Allbritton, N. L. (2012, September 7). Journal of Chromatography B.

Trapping cells on a stretchable microwell array for single-cell analysis (vol 402, pg 1065, 2012)

Analytical and Bioanalytical Chemistry, Vol. 404, pp. 2519–2519.

Benchtop micromolding of polystyrene by soft lithography

Lab on a Chip, 11(18), 3089–3097.

Continuous analysis of dye-loaded, single cells on a microfluidic chip

Lab on a Chip, 11(7), 1333–1341.

Femtomole SHAPE Reveals Regulatory Structures in the Authentic XMRV RNA Genome

Grohman, J. K., Kottegoda, S., Gorelick, R. J., Allbritton, N. L., & Weeks, K. M. (2011, November 29). Journal of the American Chemical Society.

Isolation and manipulation of living adherent cells by micromolded magnetic rafts

Biomicrofluidics, 5(3).

Kinetic analysis of PI3K reactions with fluorescent PIP2 derivatives

Huang, W., Jiang, D., Wang, X., Wang, K., Sims, C. E., Allbritton, N. L., & Zhang, Q. (2011, July 25). Analytical and Bioanalytical Chemistry.

Measuring enzyme activity in single cells

Kovarik, M. L., & Allbritton, N. L. (2011, February 12). Trends in Biotechnology.

Micro Total Analysis Systems for Cell Biology and Biochemical Assays

Kovarik, M. L., Gach, P. C., Ornoff, D. M., Wang, Y., Balowski, J., Farrag, L., & Allbritton, N. L. (2011, October 3). Analytical Chemistry.

Patterning pallet arrays for cell selection based on high-resolution measurements of fluorescent biosensors

Shadpour, H., Zawistowski, J. S., Herman, A., Hahn, K., & Allbritton, N. L. (2011, April 19). Analytica Chimica Acta.

Polystyrene-coated micropallets for culture and separation of primary muscle cells

Detwiler, D. A., Dobes, N. C., Sims, C. E., Kornegay, J. N., & Allbritton, N. L. (2011, December 8). Analytical and Bioanalytical Chemistry.

Sample transport and electrokinetic injection in a microchip device for chemical cytometry

Kovarik, M. L., Lai, H. H., Xiong, J. C., & Allbritton, N. L. (2011, October 20). Electrophoresis.

Single-cell analysis of phosphoinositide 3-kinase and phosphatase and tensin homolog activation

Faraday Discussions, 149, 187–200.

Trapping cells on a stretchable microwell array for single-cell analysis

Wang, Y., Shah, P., Phillips, C., Sims, C. E., & Allbritton, N. L. (2011, November 16). Analytical and Bioanalytical Chemistry.

Use of a virtual wall valve in polydimethylsiloxane microfluidic devices for bioanalytical applications

Biomicrofluidics, 5(2).

Air-stable supported membranes for single-cell cytometry on PDMS microchips

Lab on a Chip, 10(7), 864–870.

Contact printing of arrayed microstructures

Xu, W., Luikart, A. M., Sims, C. E., & Allbritton, N. L. (2010, April 27). Analytical and Bioanalytical Chemistry.

In situ Roughening of Polymeric Microstructures

Shadpour, H., & Allbritton, N. L. (2010, March 23). ACS Applied Materials & Interfaces.

Microcup Arrays for the Efficient Isolation and Cloning of Cells

Xu, W., Sims, C. E., & Allbritton, N. L. (2010, March 3). Analytical Chemistry.