Rebecca A. Slotkowski

Works (5)

Updated: August 26th, 2023 21:16
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2020 journal article

A positive, growth-based PAM screen identifies noncanonical motifs recognized by the S. pyogenes Cas9

SCIENCE ADVANCES, 6(29).

By: D. Collias n, R. Leenay n, R. Slotkowski n, Z. Zuo *, S. Collins n, B. McGirr n, J. Liu*, C. Beisel n

co-author countries: China 🇨🇳 Germany 🇩🇪 United States of America 🇺🇸
Source: Web Of Science
Added: August 10, 2020

2019 article

CRATES: A one-step assembly method for Class 2 CRISPR arrays

TUMOR IMMUNOLOGY AND IMMUNOTHERAPY - MOLECULAR METHODS, Vol. 629, pp. 493–511.

By: C. Liao *, R. Slotkowski n & C. Beisel n

co-author countries: Germany 🇩🇪 United States of America 🇺🇸
MeSH headings : CRISPR-Cas Systems / genetics; Clustered Regularly Interspaced Short Palindromic Repeats / genetics; DNA Cleavage; Escherichia coli / genetics; Gene Editing / methods; Plasmids / genetics
Source: Web Of Science
Added: June 29, 2020

2019 journal article

Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis

NATURE COMMUNICATIONS, 10.

By: C. Liao  n, F. Ttofali n, R. Slotkowski n, S. Denny n, T. Cecil n, R. Leenay n, A. Keung n, C. Beisel n

co-author countries: Germany 🇩🇪 United States of America 🇺🇸
MeSH headings : Base Sequence; CRISPR-Associated Proteins / metabolism; Clustered Regularly Interspaced Short Palindromic Repeats / genetics; DNA / genetics; Endonucleases / metabolism; Gene Library; Genetic Techniques; HEK293 Cells; Humans; Nucleic Acid Conformation; Plasmids / metabolism; RNA / biosynthesis; RNA, Messenger / genetics; RNA, Messenger / metabolism; Saccharomyces cerevisiae / metabolism
Source: Web Of Science
Added: July 22, 2019

2019 journal article

The Francisella novicida Cas12a is sensitive to the structure downstream of the terminal repeat in CRISPR arrays

RNA BIOLOGY, 16(4), 404–412.

By: C. Liao  n, R. Slotkowski n, T. Achmedov* & C. Beisel n

co-author countries: Germany 🇩🇪 United States of America 🇺🇸
author keywords: Cpf1; CRISPR; RNA structure; terminator; TXTL
MeSH headings : CRISPR-Associated Proteins / metabolism; Clustered Regularly Interspaced Short Palindromic Repeats / genetics; DNA Cleavage; DNA, Intergenic / genetics; Francisella / genetics; Nucleic Acid Conformation; RNA Processing, Post-Transcriptional / genetics; RNA, Guide, Kinetoplastida / metabolism; Rho Factor / metabolism; Terminal Repeat Sequences / genetics; Transcription Termination, Genetic
Source: Web Of Science
Added: May 20, 2019

2016 journal article

Identifying and Visualizing Functional PAM Diversity across CRISPR-Cas Systems

MOLECULAR CELL, 62(1), 137–147.

By: R. Leenay n, K. Maksimchuk n, R. Slotkowski n, R. Agrawal n, A. Gomaa n, A. Briner n, R. Barrangou n , C. Beisel n

co-author countries: Egypt 🇪🇬 United States of America 🇺🇸
MeSH headings : Bacillus / chemistry; Bacillus / metabolism; CRISPR-Associated Proteins / chemistry; CRISPR-Associated Proteins / genetics; CRISPR-Cas Systems; Escherichia coli / chemistry; Escherichia coli / metabolism; Francisella / chemistry; Francisella / metabolism; High-Throughput Screening Assays / methods; Protein Structure, Tertiary; Streptococcus thermophilus / chemistry; Streptococcus thermophilus / metabolism
Sources: Web Of Science, ORCID
Added: August 6, 2018