Rebecca A. Slotkowski

Works (5)

Updated: August 26th, 2023 21:16

2020 journal article

A positive, growth-based PAM screen identifies noncanonical motifs recognized by the S. pyogenes Cas9

SCIENCE ADVANCES, 6(29).

By: D. Collias n, R. Leenay n, R. Slotkowski n, Z. Zuo *, S. Collins n, B. McGirr n, J. Liu *, C. Beisel n

TL;DR: It is reported that SpyCas9 also recognizes sequences within an N(A/C/T)GG motif, which could have implications for precise editing, off-target predictions, and CRISPR-based immunity. (via Semantic Scholar)
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Source: Web Of Science
Added: August 10, 2020

2019 article

CRATES: A one-step assembly method for Class 2 CRISPR arrays

TUMOR IMMUNOLOGY AND IMMUNOTHERAPY - MOLECULAR METHODS, Vol. 629, pp. 493–511.

By: C. Liao *, R. Slotkowski n & C. Beisel*

MeSH headings : CRISPR-Cas Systems / genetics; Clustered Regularly Interspaced Short Palindromic Repeats / genetics; DNA Cleavage; Escherichia coli / genetics; Gene Editing / methods; Plasmids / genetics
TL;DR: The cloning method CRATES (CRISPR Assembly through Trimmed Ends of Spacers) is reported, expected to simplify the study of natural CRISPR arrays and facilitate multiplexed targeting with programmable nucleases from Class 2 Cas nucleases across the myriad applications of CRISpr technologies. (via Semantic Scholar)
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Source: Web Of Science
Added: June 29, 2020

2019 journal article

Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis

NATURE COMMUNICATIONS, 10.

By: C. Liao  n, F. Ttofali n, R. Slotkowski n, S. Denny n, T. Cecil n, R. Leenay n, A. Keung n , C. Beisel*

MeSH headings : Base Sequence; CRISPR-Associated Proteins / metabolism; Clustered Regularly Interspaced Short Palindromic Repeats / genetics; DNA / genetics; Endonucleases / metabolism; Gene Library; Genetic Techniques; HEK293 Cells; Humans; Nucleic Acid Conformation; Plasmids / metabolism; RNA / biosynthesis; RNA, Messenger / genetics; RNA, Messenger / metabolism; Saccharomyces cerevisiae / metabolism
TL;DR: A modular, one-pot scheme called CRATES to construct CRISPR arrays and array libraries that can facilitate diverse multiplexing applications and help identify factors impacting crRNA biogenesis is presented. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: July 22, 2019

2019 journal article

The Francisella novicida Cas12a is sensitive to the structure downstream of the terminal repeat in CRISPR arrays

RNA BIOLOGY, 16(4), 404–412.

By: C. Liao  n, R. Slotkowski n, T. Achmedov* & C. Beisel n

author keywords: Cpf1; CRISPR; RNA structure; terminator; TXTL
MeSH headings : CRISPR-Associated Proteins / metabolism; Clustered Regularly Interspaced Short Palindromic Repeats / genetics; DNA Cleavage; DNA, Intergenic / genetics; Francisella / genetics; Nucleic Acid Conformation; RNA Processing, Post-Transcriptional / genetics; RNA, Guide, Kinetoplastida / metabolism; Rho Factor / metabolism; Terminal Repeat Sequences / genetics; Transcription Termination, Genetic
TL;DR: It is shown that a structured hairpin located immediately downstream of the 3ʹ repeat interferes with utilization of the adjacent encoded guide RNA by Francisella novicida (Fn)Cas12a, revealing that sequences surrounding a CRISPR array can interfere with the function of aCRISPR nuclease. (via Semantic Scholar)
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Source: Web Of Science
Added: May 20, 2019

2016 journal article

Identifying and Visualizing Functional PAM Diversity across CRISPR-Cas Systems

MOLECULAR CELL, 62(1), 137–147.

By: R. Leenay n, K. Maksimchuk n, R. Slotkowski n, R. Agrawal n, A. Gomaa n, A. Briner n, R. Barrangou n , C. Beisel n

MeSH headings : Bacillus / chemistry; Bacillus / metabolism; CRISPR-Associated Proteins / chemistry; CRISPR-Associated Proteins / genetics; CRISPR-Cas Systems; Escherichia coli / chemistry; Escherichia coli / metabolism; Francisella / chemistry; Francisella / metabolism; High-Throughput Screening Assays / methods; Protein Structure, Tertiary; Streptococcus thermophilus / chemistry; Streptococcus thermophilus / metabolism
TL;DR: An in vivo, positive, and tunable screen termed PAM-SCANR (PAM screen achieved by NOT-gate repression) to elucidate functional PAMs as well as an interactive visualization scheme termed the PAM wheel to convey individual PAM sequences and their activities to accelerate ability to understand and exploit the multitude of CRISPR-Cas systems in nature. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

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