Works Published in 2001
Displaying works 21 - 40 of 50 in total
Sorted by most recent date added to the index first, which may not be the same as publication date order.
2001 journal article
Identification of a developmental gradient of estrogen receptor expression and cellular localization in the developing and adult female rat primary somatosensory cortex
Developmental Brain Research, 129(1), 39–46.
Contributors: A. Zsarnovszky* & S. Belcher*
author keywords: barrel; cortex; development; estrogen; estrogen receptor; immunohistochemistry; posteromedial barrel subfield; somatosensory
MeSH headings : Animals; Antibodies; Estrogens / metabolism; Female; Immunohistochemistry; Neurons / chemistry; Neurons / metabolism; Rats; Rats, Sprague-Dawley; Receptors, Estrogen / analysis; Receptors, Estrogen / biosynthesis; Receptors, Estrogen / immunology; Somatosensory Cortex / cytology; Somatosensory Cortex / growth & development; Somatosensory Cortex / metabolism
TL;DR:
Observations reveal a developmental transition of ER expression in the PMBS; ER alpha is expressed during early development, ER alpha and ER beta are co-expressed at later developmental times, and only ER beta is expressed in adults.
(via Semantic Scholar)
Source: ORCID
Added: March 5, 2021
2001 journal article
Early postnatal ethanol exposure selectively decreases BDNF and truncated TrkB-T2 receptor mRNA expression in the rat cerebellum
Molecular Brain Research, 93(1), 46–55.
Contributors: K. Light*, Y. Ge * & S. Belcher*
author keywords: cerebellum; truncated TrkB; TrkC; ethanol-exposure; RT-PCR; mRNA
MeSH headings : Actins / genetics; Animals; Animals, Newborn; Brain-Derived Neurotrophic Factor / genetics; Central Nervous System Depressants / pharmacology; Cerebellum / drug effects; Cerebellum / physiology; DNA Primers; Ethanol / pharmacology; Female; Gene Expression Regulation, Developmental / drug effects; Isomerism; Pregnancy; RNA, Messenger / analysis; Rats; Rats, Sprague-Dawley; Receptor, trkB / chemistry; Receptor, trkB / genetics; Receptor, trkC / genetics; Reverse Transcriptase Polymerase Chain Reaction
TL;DR:
The current study defines the impact of ethanol exposure at the onset of ethanol vulnerability on the relative concentrations of mRNA encoding the neurotrophic factor receptors TrkB and TrkC and identifies a specific decrease in overall TrkB receptor mRNA expression that is primarily a function of the TrkB-T2 receptor isoform.
(via Semantic Scholar)
Source: ORCID
Added: March 5, 2021
2001 journal article
Estrogenic actions in the brain: Estrogen, phytoestrogens, and rapid intracellular signaling mechanisms
Journal of Pharmacology and Experimental Therapeutics, 299(2), 408–414. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034768705&partnerID=MN8TOARS
Contributors: S. Belcher & A. Zsarnovszky
Source: ORCID
Added: March 5, 2021
2001 journal article
Estrogen receptor β immunoreactivity in differentiating cells of the developing rat cerebellum
Journal of Comparative Neurology, 430(3), 396–409.
Contributors: R. Jakab*, J. Wong* & S. Belcher*
author keywords: differentiation; development; glia; granule cells; growth; immunocytochemistry; migration; neurons; Purkinje cells
TL;DR:
The results suggest that ERβ may play a role in growth‐related mechanisms during differentiation of cerebellar neurons and glia during migration.
(via Semantic Scholar)
Source: ORCID
Added: March 5, 2021
2001 journal article
Homogeneous Preparations of 3′-Phosphoglycolate-Terminated Oligodeoxynucleotides from Bleomycin-Treated DNA as Verified by Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
Analytical Biochemistry, 289(2), 274–280.
MeSH headings : Alkaline Phosphatase / pharmacology; Antimetabolites, Antineoplastic / pharmacology; Bleomycin / pharmacology; Chromatography, High Pressure Liquid; DNA / drug effects; DNA Damage / drug effects; DNA Repair; Electrophoresis, Polyacrylamide Gel; Glycolates / metabolism; Mass Spectrometry; Oligonucleotides / metabolism; Spectrometry, Mass, Electrospray Ionization; Spectroscopy, Fourier Transform Infrared; Time Factors
TL;DR:
Single- and double-strand breaks bearing 3'-phosphoglycolate termini are among the most frequent lesions formed in DNA by ionizing radiation and other oxidative mutagens and could be relabeled with polynucleotide kinase to yield highly purified, high-specific-activity substrates for repair studies.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 27, 2021
2001 journal article
Genotyping of Simple and Compound Short Tandem Repeat Loci Using Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
Analytical Chemistry, 73(18), 4514–4521.
MeSH headings : Cyclotrons; Genotype; Humans; Microsatellite Repeats; Spectrometry, Mass, Electrospray Ionization / methods
TL;DR:
The utility of electrospray ionization Fourier transform ion cyclotron resonance (ESI-FIICR) mass spectrometry as a new approach for genotyping short tandem repeats (STRs) is demonstrated and is a powerful approach to characterize both simple and compound STRs beyond the capabilities of electrophoretic technologies.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021
2001 journal article
Selective, Sensitive, and Rapid Phosphopeptide Identification in Enzymatic Digests Using ESI-FTICR-MS with Infrared Multiphoton Dissociation
Analytical Chemistry, 73(14), 3305–3311.
MeSH headings : Amino Acid Sequence; Animals; Caseins / analysis; Cattle; Fourier Analysis; Infrared Rays; Molecular Sequence Data; Phosphopeptides / analysis; Receptor, Insulin / analysis; Sensitivity and Specificity; Spectrometry, Mass, Electrospray Ionization / methods
TL;DR:
The sensitivity and selectivity of phosphopeptides is shown to greatly improve when the volatile base piperidine is used to adjust the pH of the digestion of naturally phosphorylated proteins bovine alpha- and beta-casein.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021
2001 journal article
Complete sequencing of mono—deprotonated peptide nucleic acids by sustained off-resonance irradiation collision—induced dissociation
Journal of the American Society for Mass Spectrometry, 12(7), 805–809.
MeSH headings : Calibration; Chemical Phenomena; Chemistry, Physical; Mass Spectrometry; Peptide Nucleic Acids / chemistry; Sequence Analysis, DNA
TL;DR:
Complete peptide nucleic acids (PNAs) sequence information is obtained from the unimolecular decomposition of singly-charged PNA oligomers in the negative-ion mode using electrospray ionization coupled with Fourier transform ion cyclotron resonance mass spectrometry and sustained off-resonance irradiation collision induced dissociation.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021
2001 journal article
High-Mass Accuracy of Product Ions Produced by SORI-CID Using a Dual Electrospray Ionization Source Coupled with FTICR Mass Spectrometry
Analytical Chemistry, 73(6), 1247–1251.
MeSH headings : Cyclotrons; Fourier Analysis; Oligonucleotides / analysis; Spectrometry, Mass, Electrospray Ionization
TL;DR:
This internal standardization method demonstrated significantly more consistent accurate mass measurements with average mass errors ranging from -1.2 to -3.2 ppm for the 15-mer oligonucleotide used in this study.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021
2001 journal article
Genotyping short tandem repeats using flow injection and electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry
Rapid Communications in Mass Spectrometry, 15(5), 348–350.
MeSH headings : Alleles; Cyclotrons; Flow Injection Analysis; Fourier Analysis; Genotype; Homozygote; Polymerase Chain Reaction; Spectrometry, Mass, Electrospray Ionization; Tandem Repeat Sequences
TL;DR:
Characterizing polymerase chain reaction (PCR) amplicons has been accomplished for the first time using flow injection analysis coupled to electrospray ionization mass spectrometry (ESI-MS) to illustrate the compatibility of ESI-MS for genotyping short tandem repeat sequences and the potential for high-throughput genotypesing of large PCR amplicons.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021
2001 journal article
Detection of double-stranded PCR amplicons at the attomole level electrosprayed from low nanomolar solutions using FT-ICR mass spectrometry
Fresenius' Journal of Analytical Chemistry, 369(3-4), 246–251.
MeSH headings : Alleles; Cyclotrons; DNA / chemistry; DNA / genetics; Fourier Analysis; Homozygote; Humans; Mass Spectrometry / methods; Polymerase Chain Reaction / methods; Sensitivity and Specificity; Solutions; Spectrometry, Mass, Electrospray Ionization / methods; Spectrophotometry / methods
TL;DR:
A remarkable sensitivity for large PCR amplicons will dramatically improve the ability of electrospray ionization mass spectrometry to address important genetic questions for low copy number genes or when the amount of initial template is limited; the latter issue is commonly encountered in DNA forensics.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021
2001 journal article
Impact of ion cloud densities on the measurement of relative ion abundances in Fourier transform ion cyclotron resonance mass spectrometry: experimental observations of coulombically induced cyclotron radius perturbations and ion cloud dephasing rates
Journal of Mass Spectrometry, 36(2), 195–203.
author keywords: space-charge; ion cloud density; signal decay; proteomics; quantification
MeSH headings : Cyclosporine / chemistry; Ions; Spectroscopy, Fourier Transform Infrared
TL;DR:
Experimental evidence supports that it is the loss of cloud coherence that accounts for the signal loss over time, with the less dense cloud de-phasing more quickly, as the ion populations of the two investigated species near equivalence, so do their time constants.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021
2001 journal article
AGRICULTURAL EXPOSURE HISTORY AMONG AFRICAN-AMERICAN FARMERS IN GEORGIA
Journal of Toxicology and Environmental Health, Part A, 63(4), 237–241.
Contributors: J. Hoppin* , J. Guzman*, P. Tolbert* & E. Flagg*
MeSH headings : Adult; Black or African American; Aged; Aged, 80 and over; Agricultural Workers' Diseases / epidemiology; Data Collection; Georgia / epidemiology; Humans; Male; Middle Aged; Occupational Exposure / statistics & numerical data; Pesticides; Surveys and Questionnaires
TL;DR:
A pilot study was conducted to explore the breadth and diversity of farming practices over time among African-American farmers in Georgia whose exposures may differ in important ways.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
Sources: ORCID, Crossref, NC State University Libraries
Added: September 13, 2019
2001 journal article
Posttranscriptional silencing of cytochrome P4501A1 (CYP1A1) during zebrafish (Danio rerio) development
Developmental Dynamics, 222(4), 645–654.
author keywords: cytochrome P450; monooxygenase; zebrafish; Ah receptor; dioxin
MeSH headings : Aging / metabolism; Animals; Base Sequence / genetics; Cloning, Molecular; Cytochrome P-450 CYP1A1 / genetics; Cytochrome P-450 CYP1A1 / metabolism; Embryo, Nonmammalian / drug effects; Embryo, Nonmammalian / physiology; Environmental Pollutants / poisoning; Gene Expression Regulation; Gene Expression Regulation, Developmental; Gene Silencing; Molecular Sequence Data; Polychlorinated Dibenzodioxins / poisoning; RNA, Messenger / metabolism; Transcription, Genetic; Zebrafish / embryology; Zebrafish / genetics; Zebrafish / metabolism
TL;DR:
The data suggest that TCDD‐mediated induction of CYP1A1 activity is regulated differentially in developing and mature systems.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 10, 2019
2001 journal article
Green fluorescent protein (GFP) as a marker of aryl hydrocarbon receptor (AhR) function in developing zebrafish (Danio rerio).
Environmental Health Perspectives, 109(8), 845–849.
MeSH headings : Animals; Biomarkers / analysis; Cytochrome P-450 CYP1A1 / genetics; Eye Abnormalities / chemically induced; Green Fluorescent Proteins; Luminescent Proteins / biosynthesis; Models, Animal; Morphogenesis / drug effects; Polychlorinated Dibenzodioxins / toxicity; Receptors, Aryl Hydrocarbon / genetics; Spine / abnormalities; Transcription, Genetic; Transfection; Zebrafish / embryology
TL;DR:
An inducible in vivo reporter system to examine expression of the aryl hydrocarbon receptor (AhR) during development in zebrafish (Danio rerio) and whether AhR-regulated GFP expression correlated with sites of TCDD toxicity is developed.
(via Semantic Scholar)
www.ncbi.nlm.nih.gov (publisher PDF)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Sources: Crossref, NC State University Libraries
Added: August 10, 2019
2001 journal article
Corrigendum to: “The zebrafish fth1, slc3a2, men1, pc, fgf3 and cycd1 genes define two regions of conserved synteny between linkage group 7 and human chromosome 11q13” [Gene 261 (2000) 235–242]
Gene, 269(1-2), 227.
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Source: ORCID
Added: February 13, 2019
2001 conference paper
Closing gaps in the regulation of MSW landfills: Defining the end of the post-closure monitoring period and the future stability of leachate recirculation landfills
Waste Tech 2001 : February 11 - 14, 2001, Hyatt Islandia, San Diego, CA.
Source: NC State University Libraries
Added: August 6, 2018
2001 journal article
Structure of human DNMT2, an enigmatic DNA methyltransferase homolog that displays denaturant-resistant binding to DNA
Nucleic Acids Research, 29(2), 439–448.
MeSH headings : Adenosine Triphosphate / chemistry; Amino Acid Motifs; Amino Acid Sequence; Animals; Binding Sites; Computer Simulation; Conserved Sequence; Crystallography, X-Ray; DNA (Cytosine-5-)-Methyltransferases / chemistry; DNA (Cytosine-5-)-Methyltransferases / genetics; Guanosine Triphosphate / chemistry; Humans; Mice; Models, Molecular; Molecular Sequence Data; Multigene Family; Nucleic Acid Denaturation; Protein Denaturation; Protein Structure, Quaternary; Protein Structure, Secondary; Protein Structure, Tertiary; Recombinant Proteins / chemistry; Sequence Deletion; Sequence Homology, Amino Acid
TL;DR:
It is shown that homologs of DNMT2, which are present in some organisms that are not known to methylate their genomes, contain a specific target-recognizing sequence motif including an invariant CysPheThr tripeptide.
(via Semantic Scholar)
Sources: Web Of Science, Crossref, ORCID, NC State University Libraries
Added: August 6, 2018
2001 review
Novel immune-type receptor genes
[Review of ]. IMMUNOLOGICAL REVIEWS, 181(1), 250–259.
MeSH headings : Animals; Biological Evolution; Fishes / genetics; Fishes / immunology; Genetic Variation; Genome; Models, Biological; Molecular Structure; Polymerase Chain Reaction; Receptors, Immunologic / chemistry; Receptors, Immunologic / genetics
TL;DR:
The presence in NITR of V regions that are related closely to those found in Ig and TCR, as well as regulatory motifs and other structural features that are characteristic of immune inhibitory receptors encoded at the leukocyte receptor cluster, suggests that the NITRs are representative of an integral stage in the evolution of innate and adaptive immune function.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2001 journal article
Immune-type receptor genes in zebrafish share genetic and functional properties with genes encoded by the mammalian leukocyte receptor cluster
Proceedings of the National Academy of Sciences, 98(12), 6771–6776.
MeSH headings : Amino Acid Sequence; Animals; Biological Evolution; Conserved Sequence; Genes, Immunoglobulin; Genetic Linkage; Killer Cells, Natural / immunology; Molecular Sequence Data; Multigene Family; Receptors, Immunologic / genetics; Zebrafish / genetics; Zebrafish / immunology
TL;DR:
Antibody-induced crosslinking of Nitrs that have been introduced into a human natural killer cell line inhibits the phosphorylation of mitogen-activated protein kinase that is triggered by natural killer-sensitive tumor target cells.
(via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: August 6, 2018
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