Works (8)
Updated: July 23rd, 2023 19:25
2020 journal article
The Solution Structures and Interaction of SinR and SinI: Elucidating the Mechanism of Action of the Master Regulator Switch for Biofilm Formation in Bacillus subtilis
JOURNAL OF MOLECULAR BIOLOGY, 432(2), 343–357.
author keywords: Biofilms; Bacillus subtilis; Transcriptional regulation; NMR solution structure
MeSH headings : Amino Acid Sequence / genetics; Bacillus subtilis / genetics; Bacillus subtilis / ultrastructure; Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / ultrastructure; Biofilms / growth & development; DNA-Binding Proteins / chemistry; DNA-Binding Proteins / genetics; DNA-Binding Proteins / ultrastructure; Gene Expression Regulation, Bacterial / genetics; Molecular Docking Simulation; Mutation / genetics; Protein Binding / genetics; Protein Conformation
TL;DR:
This work presents the first full-length structural model of tetrameric SinR using a hybrid approach combining high-resolution solution NMR, chemical crosslinking, mass spectrometry, and molecular docking, and provides an evidence-based mechanism that confirms how disruption of the SinR tetramer by SinI regulates gene expression.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: February 27, 2020
2014 journal article
A DNA Mimic: The Structure and Mechanism of Action for the Anti-Repressor Protein AbbA
JOURNAL OF MOLECULAR BIOLOGY, 426(9), 1911–1924.
author keywords: transition state regulator; AbbA; DNA mimic; molecular docking; NMR
MeSH headings : Bacillus subtilis / chemistry; Bacillus subtilis / metabolism; Bacterial Proteins / chemistry; Bacterial Proteins / metabolism; Magnetic Resonance Spectroscopy; Models, Molecular; Molecular Docking Simulation; Protein Conformation; Protein Multimerization
TL;DR:
Interestingly, it is shown that AbbA has structural and surface characteristics that closely mimic the DNA phosphate backbone, enabling it to readily carry out its physiological function.
(via Semantic Scholar)
europepmc.org (repository)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
15. Life on Land
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2014 journal article
Chemical shift assignments and secondary structure prediction of the C-terminal domain of the response regulator BfmR from Acinetobacter baumannii
BIOMOLECULAR NMR ASSIGNMENTS, 8(1), 67–70.
author keywords: NMR; BfmR; Acinetobacter baumannii; Assignment
MeSH headings : Acinetobacter baumannii / metabolism; Bacterial Proteins / chemistry; Nuclear Magnetic Resonance, Biomolecular; Protein Structure, Secondary; Protein Structure, Tertiary
TL;DR:
Backbone and sidechain resonance assignments and secondary structure prediction for the C-terminal domain of BfmR (residues 130–238) from A. baumannii are reported.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2014 journal article
Chemical shift assignments and secondary structure prediction of the master biofilm regulator, SinR, from Bacillus subtilis
BIOMOLECULAR NMR ASSIGNMENTS, 8(1), 155–158.
author keywords: NMR; SinR; Bacillus subtilis; Biofilm regulation
MeSH headings : Bacillus subtilis / metabolism; Bacterial Proteins / chemistry; Biofilms; Nuclear Magnetic Resonance, Biomolecular; Protein Structure, Secondary
TL;DR:
The full-length backbone and side chain chemical shift values in addition to the experimentally derived secondary structure predictions are reported as the first step towards directly studying the complex interaction dynamics between SinR and SinI.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
15. Life on Land
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2014 journal article
Structure and DNA-Binding Traits of the Transition State Regulator AbrB
STRUCTURE, 22(11), 1650–1656.
MeSH headings : Bacillus subtilis / metabolism; Bacterial Proteins / chemistry; Bacterial Proteins / metabolism; Binding Sites; DNA, Bacterial / metabolism; DNA-Binding Proteins / chemistry; DNA-Binding Proteins / metabolism; Magnetic Resonance Spectroscopy; Mass Spectrometry; Molecular Docking Simulation; Protein Multimerization; Protein Structure, Secondary; Transcription Factors / chemistry; Transcription Factors / metabolism
TL;DR:
It is noted that AbrB possesses a strip of positive electrostatic potential encompassing its DNA-binding region and that its C-terminal domain aids in DNA binding.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2013 journal article
Chemical crosslinking and LC/MS analysis to determine protein domain orientation: Application to AbrB
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 431(2), 253–257.
author keywords: NMR; Solution structure; Chemical crosslinking; Domain orientation; AbrB; Gas-phase cleavable
MeSH headings : Amino Acid Sequence; Bacterial Proteins / chemistry; Chromatography, Liquid; Cross-Linking Reagents / chemistry; DNA-Binding Proteins / chemistry; Molecular Sequence Data; Protein Structure, Tertiary; Tandem Mass Spectrometry; Transcription Factors / chemistry
TL;DR:
This work solved the NMR structures of both domains independently and utilized gas-phase cleavable chemical crosslinking and LC/MS(n) analysis to correctly position the domains within the full tetrameric AbrB protein structure.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2012 journal article
H-1, C-13, and N-15 resonance assignments and secondary structure prediction of the full-length transition state regulator AbrB from Bacillus anthracis
BIOMOLECULAR NMR ASSIGNMENTS, 6(1), 95–98.
author keywords: AbrB; NMR; Bacillus anthracis; Transition state regulator
MeSH headings : Bacillus anthracis; Bacterial Proteins / chemistry; DNA-Binding Proteins / chemistry; Nuclear Magnetic Resonance, Biomolecular; Protein Structure, Secondary; Transcription Factors / chemistry
TL;DR:
Backbone and sidechain resonance assignments and secondary structure prediction for the full-length AbrB protein from B. anthracis are reported.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2012 journal article
Identification of BfmR, a Response Regulator Involved in Biofilm Development, as a Target for a 2-Aminoimidazole-Based Antibiofilm Agent
BIOCHEMISTRY, 51(49), 9776–9778.
MeSH headings : Acinetobacter baumannii / drug effects; Acinetobacter baumannii / growth & development; Bacterial Proteins / chemistry; Bacterial Proteins / physiology; Biofilms / drug effects; Imidazoles / pharmacology; Microscopy, Confocal; Models, Molecular
TL;DR:
The 2AI compound studied here represents a unique small molecule scaffold that targets bacterial response regulators and is cell permeable, while pull-down assays identified BfmR, a response regulator that is the master controller of biofilm formation, as a target for this compound.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
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