Paul Greback-Clarke

Works (2)

Updated: April 23rd, 2025 18:28

2023 journal article

Peptide ligands for the affinity purification of adeno‐associated viruses from HEK 293 cell lysates

Biotechnology and Bioengineering, 120(8), 2283–2300.

By: W. Chu n, S. Shastry n, E. Barbieri n, R. Prodromou n, P. Greback‐Clarke n, W. Smith n, B. Moore n, R. Kilgore n ...

Contributors: W. Chu n, S. Shastry n, E. Barbieri n, R. Prodromou n, P. Greback-Clarke n, W. Smith n, B. Moore n, . al.

author keywords: adeno-associated virus; affinity chromatography; HEK 293 lysate; peptide ligands; virus purification
MeSH headings : Humans; Dependovirus / genetics; HEK293 Cells; Ligands; Peptides / genetics; Peptides / metabolism; Amino Acid Sequence; Genetic Vectors
topics (OpenAlex): Virus-based gene therapy research; Viral Infectious Diseases and Gene Expression in Insects; RNA Interference and Gene Delivery
TL;DR: Peptide ligands that selectively capture AAVs and release them under mild conditions are introduced, affording high recovery, high recovery of host cell proteins (HCPs), and high transduction activity of the purified viruses. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: August 7, 2023

2023 journal article

Rational design and experimental evaluation of peptide ligands for the purification of adeno‐associated viruses via affinity chromatography

Biotechnology Journal, 19(1).

By: S. Shastry n, W. Chu n, E. Barbieri n, P. Greback‐Clarke n, W. Smith  n, C. Cummings n, A. Minzoni n, J. Pancorbo n ...

Contributors: S. Shastry n, W. Chu n, E. Barbieri n, P. Greback-Clarke n, W. Smith  n, C. Cummings n, A. Minzoni n, . al.

author keywords: adeno-associated virus; affinity chromatography; gene therapy; peptide ligands; transduction activity
MeSH headings : Humans; Dependovirus / genetics; Capsid / chemistry; HEK293 Cells; Transduction, Genetic; Peptides / metabolism; Ligands; Chromatography, Affinity; Genetic Vectors / genetics
topics (OpenAlex): Virus-based gene therapy research; Viral Infectious Diseases and Gene Expression in Insects; CAR-T cell therapy research
TL;DR: This study introduces a cohort of peptide ligands that mimic the biorecognition activity of the AAV receptor (AAVR) and anti-AAV antibody A20, enable product elution under near-physiological conditions (pH 6.0) and grant extended reusability by withstanding multiple regenerations. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: October 10, 2023

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