Raphael Prodromou

Works (10)

Updated: April 25th, 2025 16:25

2025 journal article

Light-Controlled Peptide Ligands for the Photoaffinity Purification of FLT3 and CD38

ACS Applied Engineering Materials, 3(2), 379–391.

By: B. Moore n, R. Prodromou n, G. Rusch n, C. Nguyen n, W. Chu n, V. Pozdin*, M. Daniele n , S. Menegatti n 

Contributors: B. Moore n, R. Prodromou n, G. Rusch n, C. Nguyen n, W. Chu n, V. Pozdin*, M. Daniele n , S. Menegatti n 

author keywords: azobenzene-cyclic peptides; affinity ligands; photoaffinity chromatography; biorecognition; cellmarkers
topics (OpenAlex): Photochromic and Fluorescence Chemistry; Semiconductor materials and devices; Oxidative Organic Chemistry Reactions
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Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: February 10, 2025

2023 journal article

Molecular Engineering of Cyclic Azobenzene‐Peptide Hybrid Ligands for the Purification of Human Blood Factor VIII via Photo‐Affinity Chromatography

Advanced Functional Materials, 33(14), 2213881.

By: R. Prodromou n, B. Moore n, W. Chu n, H. Deal n, A. San Miguel n , A. Brown n , M. Daniele n , V. Pozdin*, S. Menegatti n 

Contributors: R. Prodromou n, B. Moore n, W. Chu n, H. Deal n, A. Miguel n , A. Brown n , M. Daniele n , V. Pozdin*, S. Menegatti n 

author keywords: affinity ligands; azobenzene-peptide hybrids; biomolecular recognition; factor VIII; photo-affinity chromatography
topics (OpenAlex): Hemophilia Treatment and Research; Platelet Disorders and Treatments; Cancer-related gene regulation
TL;DR: The cyclic azobenzene‐peptide ligands introduced in this report demonstrate a novel route integrating gentle operational conditions in a rapid and efficient bioprocess for the purification of life‐saving biotherapeutics. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: February 20, 2023

2023 journal article

Peptide ligands for the affinity purification of adeno‐associated viruses from HEK 293 cell lysates

Biotechnology and Bioengineering, 120(8), 2283–2300.

By: W. Chu n, S. Shastry n, E. Barbieri n, R. Prodromou n, P. Greback‐Clarke n, W. Smith n, B. Moore n, R. Kilgore n ...

Contributors: W. Chu n, S. Shastry n, E. Barbieri n, R. Prodromou n, P. Greback-Clarke n, W. Smith n, B. Moore n, . al.

author keywords: adeno-associated virus; affinity chromatography; HEK 293 lysate; peptide ligands; virus purification
MeSH headings : Humans; Dependovirus / genetics; HEK293 Cells; Ligands; Peptides / genetics; Peptides / metabolism; Amino Acid Sequence; Genetic Vectors
topics (OpenAlex): Virus-based gene therapy research; Viral Infectious Diseases and Gene Expression in Insects; RNA Interference and Gene Delivery
TL;DR: Peptide ligands that selectively capture AAVs and release them under mild conditions are introduced, affording high recovery, high recovery of host cell proteins (HCPs), and high transduction activity of the purified viruses. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: August 7, 2023

2022 article

Development of peptide ligands for the purification of α-1 antitrypsin from cell culture fluids

Chu, W., Prodromou, R., Moore, B., Elhanafi, D., Kilgore, R., Shastry, S., & Menegatti, S. (2022, July 22). Journal of Chromatography A, Vol. 1679.

By: W. Chu n, R. Prodromou n, B. Moore n, D. Elhanafi n, R. Kilgore n, S. Shastry n, S. Menegatti n 

author keywords: a-1 antitrypsin; Peptide ligands; Affinity chromatography; Protein purification; CHO cell culture harvest
MeSH headings : Animals; CHO Cells; Cell Culture Techniques; Chromatography, Affinity; Cricetinae; Cricetulus; Ligands; Molecular Docking Simulation; Peptides; alpha 1-Antitrypsin
topics (OpenAlex): Protein Hydrolysis and Bioactive Peptides; Silk-based biomaterials and applications; Chemical Synthesis and Analysis
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Sources: Web Of Science, NC State University Libraries
Added: October 11, 2022

2021 journal article

Engineering Next Generation Cyclized Peptide Ligands for Light‐Controlled Capture and Release of Therapeutic Proteins

Advanced Functional Materials, 31(27), 2101410.

By: R. Prodromou n, K. Day n, S. Saberi‐Bosari n, J. Schneible n, M. Mabe  n, A. San Miguel n , M. Daniele n , V. Pozdin*, S. Menegatti n 

Contributors: R. Prodromou n, K. Day n, S. Saberi-Bosari n, J. Schneible n, M. Mabe  n, A. Miguel n , M. Daniele n , V. Pozdin*, S. Menegatti n 

author keywords: azobenzene; cyclized peptide; light‐ controlled biorecognition; microfluidic devices; photochromic switch; photo‐ isomerization kinetics
topics (OpenAlex): Chemical Synthesis and Analysis; Click Chemistry and Applications; RNA Interference and Gene Delivery
TL;DR: A framework for prototyping photo‐affinity adsorbents comprising azobenzene‐cyclized peptides (ACPs) conjugated to translucent porous beads (ChemMatrix) and it is demonstrated that the isomerization at different light intensities is correlated to the cyclization geometry. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: May 24, 2021

2020 article

Affibody-Binding Ligands

Barozzi, A., Lavoie, R. A., Day, K. N., Prodromou, R., & Menegatti, S. (2020, May 27). International Journal of Molecular Sciences, Vol. 21.

By: A. Barozzi n, R. Lavoie  n, K. Day n, R. Prodromou n & S. Menegatti n 

author keywords: affibody; peptide ligands; affinity chromatography; biomanufacturing; protein purification
MeSH headings : Amino Acid Sequence; Humans; Immunoglobulin G / metabolism; Ligands; Molecular Docking Simulation; Peptide Library; Peptides / chemistry; Peptides / metabolism; Recombinant Fusion Proteins / metabolism; Serum Albumin, Human / metabolism; Temperature
topics (OpenAlex): Monoclonal and Polyclonal Antibodies Research; Transgenic Plants and Applications; Protein purification and stability
TL;DR: This work presents the development of affibody-binding peptides to be utilized as ligands for their purification from bacterial lysates and demonstrates that IGKQRI can be employed as ligand in affibODY purification processes. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: July 20, 2020

2020 article

Peptides and pseudopeptide ligands: a powerful toolbox for the affinity purification of current and next-generation biotherapeutics

Chu, W., Prodromou, R., Day, K. N., Schneible, J. D., Bacon, K. B., Bowen, J. D., … Menegatti, S. (2020, October 23). Journal of Chromatography A, Vol. 1635.

By: W. Chu n, R. Prodromou n, K. Day n, J. Schneible n, K. Bacon n, J. Bowen n, R. Kilgore n, C. Catella n ...

author keywords: Affinity ligands; peptides; peptoids; pseudopeptides; biotherapeutics
MeSH headings : Antibodies / isolation & purification; Biological Products / isolation & purification; Chemistry, Pharmaceutical / methods; Chromatography, Affinity; Family Characteristics; Humans; Ligands; Peptides / isolation & purification; Peptoids / chemistry; Proteins / isolation & purification
topics (OpenAlex): Monoclonal and Polyclonal Antibodies Research; Chemical Synthesis and Analysis; Click Chemistry and Applications
TL;DR: This work presents a comprehensive and systematic review of the literature on peptide-based ligands and their use in the affinity purification of established and upcoming biological drugs. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: January 25, 2021

2020 journal article

Photoinduced reconfiguration to control the protein-binding affinity of azobenzene-cyclized peptides

Journal of Materials Chemistry B, 8(33), 7413–7427.

By: K. Day n, J. Schneible n, A. Young n, V. Pozdin n, G. Van Den Driessche  n, L. Gaffney n, R. Prodromou n, D. Freytes n  ...

Contributors: K. Day n, J. Schneible n, A. Young n, V. Pozdin n, G. Driessche  n, L. Gaffney n, R. Prodromou n, . al.

MeSH headings : Amino Acid Sequence; Azo Compounds / chemistry; Hydrogen-Ion Concentration; Isomerism; Osmolar Concentration; Peptides, Cyclic / chemistry; Photochemical Processes
topics (OpenAlex): Photochromic and Fluorescence Chemistry; Click Chemistry and Applications; Chemical Synthesis and Analysis
TL;DR: This study demonstrates a general method to develop azobenzene-cyclized peptide ligands with light-controlled affinity for target proteins by designing photo-switchable peptides targeting vascular cell adhesion marker 1 (VCAM1), a cell marker implicated in stem cell function. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: September 14, 2020

2019 article

Affordable Microfluidic Bead-Sorting Platform for Automated Selection of Porous Particles Functionalized with Bioactive Compounds

Saberi-Bosari, S., Omary, M., Lavoie, A., Prodromou, R., Day, K., Menegatti, S., & San-Miguel, A. (2019, May 10). Scientific Reports, Vol. 9.

By: S. Saberi-Bosari n, M. Omary  n, A. Lavoie n, R. Prodromou n, K. Day n, S. Menegatti n , A. San-Miguel n 

MeSH headings : Combinatorial Chemistry Techniques / instrumentation; Humans; Image Processing, Computer-Assisted; Immunoglobulin G / chemistry; Lab-On-A-Chip Devices; Microfluidic Analytical Techniques / instrumentation; Peptide Library; Porosity; Proof of Concept Study; Unsupervised Machine Learning
topics (OpenAlex): Innovative Microfluidic and Catalytic Techniques Innovation; Monoclonal and Polyclonal Antibodies Research; Advanced Biosensing Techniques and Applications; Microfluidic and Capillary Electrophoresis Applications; Microfluidic and Bio-sensing Technologies
TL;DR: A low-cost microfluidics-based platform enabling automated screening of small porous beads from solid-phase peptide libraries with high sensitivity and specificity, to identify leads with high binding affinity for a biological target is presented. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID
Added: May 28, 2019

2019 article

Discovery and Evaluation of Peptide Ligands for Selective Adsorption and Release of Cas9 Nuclease on Solid Substrates

Day, K., Prodromou, R., Bosari, S. S., Lavoie, A., Omary, M., Market, C., … Menegatti, S. (2019, November 22). Bioconjugate Chemistry, Vol. 30, pp. 3057–3068.

By: K. Day n, R. Prodromou n, S. Bosari n, A. Lavoie n, M. Omary  n, C. Market n, A. Miguel n , S. Menegatti n 

MeSH headings : Adsorption; Amino Acid Sequence; Binding Sites; CRISPR-Associated Protein 9 / isolation & purification; CRISPR-Associated Protein 9 / metabolism; Drug Discovery; Ligands; Molecular Docking Simulation; Peptide Library; Peptides / metabolism; Resins, Synthetic / chemistry; Substrate Specificity
topics (OpenAlex): CRISPR and Genetic Engineering; Advanced biosensing and bioanalysis techniques; RNA Interference and Gene Delivery
TL;DR: This work represents the first effort at developing short synthetic Cas9-binding peptides and demonstrates their applicability as affinity ligands for the purification of a Cas nuclease. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID
Added: January 13, 2020

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