Raphael Prodromou

Works (10)

Updated: February 17th, 2025 07:37

2025 article

Light-Controlled Peptide Ligands for the Photoaffinity Purification of FLT3 and CD38

Moore, B. D., Prodromou, R., Rusch, G. P., Nguyen, C., Chu, W., Pozdin, V., … Menegatti, S. (2025, January 31). ACS APPLIED ENGINEERING MATERIALS, Vol. 1.

By: B. Moore n, R. Prodromou n, G. Rusch n, C. Nguyen n, W. Chu n, V. Pozdin*, M. Daniele n , S. Menegatti n 

author keywords: azobenzene-cyclic peptides; affinity ligands; photoaffinity chromatography; biorecognition; cellmarkers
Sources: Web Of Science, NC State University Libraries
Added: February 10, 2025

2023 journal article

Molecular Engineering of Cyclic Azobenzene‐Peptide Hybrid Ligands for the Purification of Human Blood Factor VIII via Photo‐Affinity Chromatography

Prodromou, R., Moore, B. D., Chu, W., Deal, H., San Miguel, A., Brown, A. C., … Menegatti, S. (2023, January 25). Molecular Engineering of Cyclic Azobenzene-Peptide Hybrid Ligands for the Purification of Human Blood Factor VIII via Photo-Affinity Chromatography. ADVANCED FUNCTIONAL MATERIALS, Vol. 1.

By: R. Prodromou n, B. Moore n, W. Chu n, H. Deal n, A. San Miguel n , A. Brown n , M. Daniele n , V. Pozdin*, S. Menegatti n 

author keywords: affinity ligands; azobenzene-peptide hybrids; biomolecular recognition; factor VIII; photo-affinity chromatography
TL;DR: The cyclic azobenzene‐peptide ligands introduced in this report demonstrate a novel route integrating gentle operational conditions in a rapid and efficient bioprocess for the purification of life‐saving biotherapeutics. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: February 20, 2023

2023 article

Peptide ligands for the affinity purification of adeno-associated viruses from HEK 293 cell lysates

Chu, W., Shastry, S., Barbieri, E., Prodromou, R., Greback-Clarke, P., Smith, W., … Menegatti, S. (2023, July 12). BIOTECHNOLOGY AND BIOENGINEERING, Vol. 7.

By: W. Chu n, S. Shastry n, E. Barbieri n, R. Prodromou n, P. Greback-Clarke n, W. Smith n, B. Moore n, R. Kilgore n ...

author keywords: adeno-associated virus; affinity chromatography; HEK 293 lysate; peptide ligands; virus purification
MeSH headings : Humans; Dependovirus / genetics; HEK293 Cells; Ligands; Peptides / genetics; Peptides / metabolism; Amino Acid Sequence; Genetic Vectors
TL;DR: Peptide ligands that selectively capture AAVs and release them under mild conditions are introduced, affording high recovery, high recovery of host cell proteins (HCPs), and high transduction activity of the purified viruses. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 7, 2023

2022 journal article

Development of peptide ligands for the purification of a-1 antitrypsin from cell culture fluids

JOURNAL OF CHROMATOGRAPHY A, 1679.

By: W. Chu n, R. Prodromou n, B. Moore n, D. Elhanafi n, R. Kilgore n, S. Shastry n, S. Menegatti n 

author keywords: a-1 antitrypsin; Peptide ligands; Affinity chromatography; Protein purification; CHO cell culture harvest
MeSH headings : Animals; CHO Cells; Cell Culture Techniques; Chromatography, Affinity; Cricetinae; Cricetulus; Ligands; Molecular Docking Simulation; Peptides; alpha 1-Antitrypsin
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Sources: Web Of Science, NC State University Libraries
Added: October 11, 2022

2021 journal article

Engineering Next Generation Cyclized Peptide Ligands for Light-Controlled Capture and Release of Therapeutic Proteins

ADVANCED FUNCTIONAL MATERIALS, 31(27).

By: R. Prodromou n, K. Day n, S. Saberi-Bosari n, J. Schneible n, M. Mabe  n, A. San Miguel n , M. Daniele n , V. Pozdin*, S. Menegatti n 

author keywords: azobenzene; cyclized peptide; light‐ controlled biorecognition; microfluidic devices; photochromic switch; photo‐ isomerization kinetics
TL;DR: A framework for prototyping photo‐affinity adsorbents comprising azobenzene‐cyclized peptides (ACPs) conjugated to translucent porous beads (ChemMatrix) and it is demonstrated that the isomerization at different light intensities is correlated to the cyclization geometry. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID
Added: May 24, 2021

2021 journal article

Peptides and pseudopeptide ligands: a powerful toolbox for the affinity purification of current and next-generation biotherapeutics

JOURNAL OF CHROMATOGRAPHY A, 1635.

By: W. Chu n, R. Prodromou n, K. Day n, J. Schneible n, K. Bacon n, J. Bowen n, R. Kilgore n, C. Catella n ...

author keywords: Affinity ligands; peptides; peptoids; pseudopeptides; biotherapeutics
MeSH headings : Antibodies / isolation & purification; Biological Products / isolation & purification; Chemistry, Pharmaceutical / methods; Chromatography, Affinity; Family Characteristics; Humans; Ligands; Peptides / isolation & purification; Peptoids / chemistry; Proteins / isolation & purification
TL;DR: This work presents a comprehensive and systematic review of the literature on peptide-based ligands and their use in the affinity purification of established and upcoming biological drugs. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: January 25, 2021

2020 journal article

Affibody-Binding Ligands

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 21(11).

By: A. Barozzi n, R. Lavoie  n, K. Day n, R. Prodromou n & S. Menegatti n 

author keywords: affibody; peptide ligands; affinity chromatography; biomanufacturing; protein purification
MeSH headings : Amino Acid Sequence; Humans; Immunoglobulin G / metabolism; Ligands; Molecular Docking Simulation; Peptide Library; Peptides / chemistry; Peptides / metabolism; Recombinant Fusion Proteins / metabolism; Serum Albumin, Human / metabolism; Temperature
TL;DR: This work presents the development of affibody-binding peptides to be utilized as ligands for their purification from bacterial lysates and demonstrates that IGKQRI can be employed as ligand in affibODY purification processes. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: July 20, 2020

2020 journal article

Photoinduced reconfiguration to control the protein-binding affinity of azobenzene-cyclized peptides

JOURNAL OF MATERIALS CHEMISTRY B, 8(33), 7413–7427.

By: K. Day n, J. Schneible n, A. Young n, V. Pozdin*, G. Driessche  n, L. Gaffney n, R. Prodromou n, D. Freytes n ...

MeSH headings : Amino Acid Sequence; Azo Compounds / chemistry; Hydrogen-Ion Concentration; Isomerism; Osmolar Concentration; Peptides, Cyclic / chemistry; Photochemical Processes
TL;DR: This study demonstrates a general method to develop azobenzene-cyclized peptide ligands with light-controlled affinity for target proteins by designing photo-switchable peptides targeting vascular cell adhesion marker 1 (VCAM1), a cell marker implicated in stem cell function. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID
Added: September 14, 2020

2019 journal article

Affordable Microfluidic Bead-Sorting Platform for Automated Selection of Porous Particles Functionalized with Bioactive Compounds

SCIENTIFIC REPORTS, 9(1).

By: S. Saberi-Bosari n, M. Omary  n, A. Lavoie n, R. Prodromou n, K. Day n, S. Menegatti n , A. San-Miguel  n

MeSH headings : Combinatorial Chemistry Techniques / instrumentation; Humans; Image Processing, Computer-Assisted; Immunoglobulin G / chemistry; Lab-On-A-Chip Devices; Microfluidic Analytical Techniques / instrumentation; Peptide Library; Porosity; Proof of Concept Study; Unsupervised Machine Learning
TL;DR: A low-cost microfluidics-based platform enabling automated screening of small porous beads from solid-phase peptide libraries with high sensitivity and specificity, to identify leads with high binding affinity for a biological target is presented. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID
Added: May 28, 2019

2019 journal article

Discovery and Evaluation of Peptide Ligands for Selective Adsorption and Release of Cas9 Nuclease on Solid Substrates

BIOCONJUGATE CHEMISTRY, 30(12), 3057–3068.

By: K. Day n, R. Prodromou n, S. Bosari n, A. Lavoie n, M. Omary  n, C. Market n, A. San Miguel n , S. Menegatti n 

MeSH headings : Adsorption; Amino Acid Sequence; Binding Sites; CRISPR-Associated Protein 9 / isolation & purification; CRISPR-Associated Protein 9 / metabolism; Drug Discovery; Ligands; Molecular Docking Simulation; Peptide Library; Peptides / metabolism; Resins, Synthetic / chemistry; Substrate Specificity
TL;DR: This work represents the first effort at developing short synthetic Cas9-binding peptides and demonstrates their applicability as affinity ligands for the purification of a Cas nuclease. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID
Added: January 13, 2020

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