Susan H Bernacki

Works (15)

Updated: April 5th, 2024 04:51

2019 journal article

Primary Cilia Exhibit Mechanosensitivity to Cyclic Tensile Strain and Lineage-Dependent Expression in Adipose-Derived Stem Cells

SCIENTIFIC REPORTS, 9.

By: J. Bodle n, M. Hamouda n, S. Cai n, R. Williams n, S. Bernacki n & E. Loboa*

MeSH headings : Adipocytes / cytology; Adipocytes / metabolism; Adipogenesis / genetics; Cell Differentiation / genetics; Cell Lineage / genetics; Cells, Cultured; Cilia / genetics; Core Binding Factor Alpha 1 Subunit / genetics; Gene Expression Regulation, Developmental / genetics; Humans; Mechanotransduction, Cellular / genetics; Mesenchymal Stem Cells / cytology; Mesenchymal Stem Cells / metabolism; Osteogenesis / genetics; Tensile Strength; Tissue Engineering
TL;DR: This study demonstrates that hASC primary cilia exhibit mechanosensitivity to cyclic tensile strain and lineage-dependent expression, which may in part regulate signaling pathways localized to the primary cilium during the differentiation process. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: June 17, 2019

2016 journal article

Facilitating Teamwork in Adolescent and Young Adult Oncology

JOURNAL OF ONCOLOGY PRACTICE, 12(11), 1067-+.

By: R. Johnson*, C. Macpherson*, A. Smith *, R. Block * & J. Keyton *

MeSH headings : Adolescent; Adult; Communication; Cooperative Behavior; Humans; Interprofessional Relations; Male; Patient Care Team / organization & administration; Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / drug therapy; Trust; Young Adult
TL;DR: A case of a young adult patient in the days immediately after a cancer diagnosis illustrates the critical importance of three interrelated core coordinating mechanisms-closed-loop communication, shared mental models, and mutual trust-of teamwork in an adolescent and young adult multidisciplinary oncology team. (via Semantic Scholar)
UN Sustainable Development Goal Categories
4. Quality Education (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 journal article

Novel genipin-collagen immobilization of polylactic acid (PLA) fibers for use as tissue engineering scaffolds

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 103(6), 1188–1197.

By: N. Tambe n, J. Di n, Z. Zhang *, S. Bernacki n, A. El-Shafei n  & M. King n 

Contributors: N. Tambe n, J. Di n, Z. Zhang *, S. Bernacki n, A. El-Shafei n  & M. King n 

author keywords: collagen immobilization; genipin; scaffold; functionalization; surface modification; polylactic acid (PLA) fibers
MeSH headings : Cells, Cultured; Fibroblasts / cytology; Fibroblasts / metabolism; Humans; Iridoids / chemistry; Lactic Acid / chemistry; Materials Testing; Polyesters; Polymers / chemistry; Tissue Engineering; Tissue Scaffolds / chemistry
TL;DR: The collagen was immobilized on the surface of functionalized PLA via a novel natural cross-linking moleculegenipin which resulted in improved cell proliferation of human dermal fibroblasts as compared to the PLA surface coated with collagen without genipin. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2014 journal article

Age-Related Effects on the Potency of Human Adipose-Derived Stem Cells: Creation and Evaluation of Superlots and Implications for Musculoskeletal Tissue Engineering Applications

Tissue Engineering Part C: Methods, 20(12), 972–983.

By: J. Bodle n, S. Teeter n , B. Hluck n, J. Hardin n, S. Bernacki n & E. Loboa n

MeSH headings : Adipogenesis; Adipose Tissue / cytology; Adult; Aged; Aged, 80 and over; Aging / physiology; Cell Line; Cell Proliferation; Cell Separation; DNA / metabolism; Female; Humans; Middle Aged; Musculoskeletal System / metabolism; Osteogenesis; Postmenopause; Premenopause; Proteins / metabolism; Reproducibility of Results; Stem Cells / cytology; Tissue Engineering / methods; Young Adult
TL;DR: In general, superlots effectively represented the average differentiation behavior of each of their contributing cell populations and could provide a powerful tool for increasing experimental throughput to more efficiently utilize resources when studying hASC differentiation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: August 6, 2018

2014 journal article

In vitro dermal and epidermal cellular response to titanium alloy implants fabricated with electron beam melting

MEDICAL ENGINEERING & PHYSICS, 36(10), 1367–1372.

By: J. Springer n, O. Harrysson n , D. Marcellin-Little n & S. Bernacki n

author keywords: Keratinocyte; Fibroblast; Additive manufacturing; Electron beam melting; Titanium alloy; Biocompatibility
MeSH headings : Alloys; Cell Proliferation / drug effects; Cell Survival / drug effects; Dermis / cytology; Electrons; Epidermal Cells; Fibroblasts / cytology; Fibroblasts / drug effects; Humans; Keratinocytes / cytology; Keratinocytes / drug effects; Materials Testing; Osseointegration / drug effects; Phase Transition; Prostheses and Implants; Surface Properties; Titanium / chemistry; Titanium / pharmacology
TL;DR: Electron beam melting EBM Ti6Al4V discs exhibited an acceptable biocompatibility profile compared to solid titanium alloy discs from a commercial source for dermal and epidermal cells, and may be considered as an option for fabrication of custom transdermal implants. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 journal article

Mammalian Cell Viability in Electrospun Composite Nanofiber Structures

MACROMOLECULAR BIOSCIENCE, 11(10), 1346–1356.

By: M. Canbolat n, C. Tang n, S. Bernacki n, B. Pourdeyhimi n  & S. Khan n 

author keywords: 3T3-L1 mice fibroblasts; electrospinning; nanofibers; fibers; nanotechnology; tissue engineering; viability
MeSH headings : 3T3-L1 Cells; Animals; Cell Adhesion / drug effects; Cell Death / drug effects; Cell Survival / drug effects; Fibroblasts / cytology; Fibroblasts / ultrastructure; Mammals / metabolism; Mice; Nanocomposites / chemistry; Nanocomposites / ultrastructure; Nanofibers / chemistry; Nanofibers / ultrastructure; Polyesters / pharmacology; Polyvinyl Alcohol / pharmacology; Solutions; Solvents; Time Factors; Tissue Engineering / methods
TL;DR: It is observed that cells do not survive cell electrospinning but survive cell layering, and dehydration and fiber stretching are the main causes of cell death. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2010 journal article

Effect of Varied Ionic Calcium on Human Adipose-Derived Stem Cell Mineralization

TISSUE ENGINEERING PART A, 16(6), 1971–1981.

By: S. McCullen n, J. Zhan*, M. Onorato n, S. Bernacki n & E. Loboa n

MeSH headings : Adipocytes / cytology; Calcium / pharmacology; Cell Differentiation / drug effects; Cell Proliferation / drug effects; Humans; Osteogenesis / drug effects; Spectroscopy, Fourier Transform Infrared; Stem Cells / cytology; Stem Cells / drug effects; Stem Cells / metabolism; Tissue Engineering / methods; X-Ray Diffraction
TL;DR: Ionic calcium should be considered a potent regulator in hASC mineralization and could serve as a potential treatment for inducing prompt ossification of hASC-seeded scaffolds for bone tissue engineering prior to implantation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2010 journal article

The Development and Validation of a LIPUS System With Preliminary Observations of Ultrasonic Effects on Human Adult Stem Cells

IEEE TRANSACTIONS ON ULTRASONICS FERROELECTRICS AND FREQUENCY CONTROL, 57(9), 1977–1984.

By: S. Marvel*, S. Okrasinski*, S. Bernacki*, E. Loboa* & P. Dayton*

MeSH headings : Adipose Tissue / cytology; Adult; Adult Stem Cells / cytology; Adult Stem Cells / diagnostic imaging; Bone Marrow Cells / cytology; Calcium / analysis; Calcium / metabolism; Cell Growth Processes / physiology; Cells, Cultured; DNA / chemistry; Humans; Reproducibility of Results; Ultrasonics / instrumentation; Ultrasonics / methods; Ultrasonography
TL;DR: The custom LIPUS system designed and validated by exploring the effects of using different pulse repetition frequency (PRF) parameters on human adipose derived adult stem cells and bone marrow derived mesenchymal stem cells was successfully designed to allow extreme parameter variation, specifically PRF, and encourages further studies. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2009 journal article

Osteogenic Effects of Rest Inserted and Continuous Cyclic Tensile Strain on hASC Lines with Disparate Osteodifferentiation Capabilities

ANNALS OF BIOMEDICAL ENGINEERING, 37(5), 955–965.

By: A. Hanson n, S. Marvel n, S. Bernacki n, A. Banes n, J. Aalst* & E. Loboa n

author keywords: Adipose derived stem cells; Mesenchymal stem cells; Osteogenesis; Mechanical effects on cells and tissues; Tissue engineering
MeSH headings : Adipose Tissue / cytology; Adult Stem Cells; Calcium / metabolism; Cell Differentiation; Cell Proliferation; Cells, Cultured; Humans; Mesenchymal Stem Cells; Models, Biological; Osteogenesis / physiology; Stress, Mechanical; Tensile Strength; Tissue Engineering / methods
TL;DR: Results showed that both continuous and rest inserted cyclic tensile strain increased the amount and rate of calcium deposition for both high and low calcium depositing hASC lines as compared to unstrained controls, suggesting that mechanical loading has a greater effect on cell lines that already have an innate ability to produce bone. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2008 journal article

Cellular Incorporation Into Electrospun Nanofibers

Annals of Plastic Surgery, 60(5), 577–583.

By: J. van Aalst, C. Reed, L. Han, T. Andrady, M. Hromadka, S. Bernacki*, K. Kolappa, J. Collins, E. Loboa*

author keywords: nanofibers; fibroblasts; adipose-derived adult stem cells; electrospinning; nanotechnology
MeSH headings : Cell Proliferation; Cell Survival; Fibroblasts / physiology; Humans; Nanostructures / chemistry; Polymers; Tissue Engineering; Tissue Scaffolds
TL;DR: Both fibroblasts and hADAS survived the electrospinning process and were incorporated into PVA nanofibers, showing retained ability to undergo chondrogenesis and maintained viability, proliferation, and function. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 6, 2018

2008 review

Isolation of human mesenchymal stem cells from bone and adipose tissue

[Review of ]. Stem cell culture, 86, 257–278.

By: S. Bernacki, M. Wall & E. Loboa

Source: NC State University Libraries
Added: August 6, 2018

2007 journal article

Characterization of electrospun nanocomposite scaffolds and biocompatibility with adipose-derived human mesenchymal stem cells

International Journal of Nanomedicine, 2(2), 253–263.

By: S. McCullen, D. Stevens, W. Roberts, L. Clarke , S. Bernacki, R. Gorga , E. Loboa

Source: NC State University Libraries
Added: August 6, 2018

2007 journal article

Differential effects on messenger ribonucleic acid expression by bone marrow-derived human mesenchymal stem cells seeded in agarose constructs due to ramped and steady applications of cyclic hydrostatic pressure

TISSUE ENGINEERING, 13(6), 1151–1158.

By: A. Finger n, C. Sargent n, K. Dulaney n, S. Bernacki n & E. Loboa n

MeSH headings : Adolescent; Adult; Biocompatible Materials / chemistry; Bone Marrow Cells / cytology; Bone Marrow Cells / drug effects; Bone Marrow Cells / metabolism; Cell Culture Techniques / methods; Cells, Cultured; Chondrocytes / cytology; Chondrocytes / drug effects; Chondrocytes / metabolism; Chondrogenesis / drug effects; Chondrogenesis / physiology; Cytoskeletal Proteins / genetics; Cytoskeletal Proteins / metabolism; Gene Expression Regulation, Developmental / drug effects; Gene Expression Regulation, Developmental / physiology; Humans; Male; Mechanotransduction, Cellular / drug effects; Mechanotransduction, Cellular / physiology; Mesenchymal Stem Cells / cytology; Mesenchymal Stem Cells / drug effects; Mesenchymal Stem Cells / metabolism; Pressure; RNA, Messenger / metabolism; Sepharose / chemistry; Tissue Engineering / methods; Transforming Growth Factor beta / administration & dosage
TL;DR: Findings indicate that hydrostatic pressure may induce chondrogenesis in hMSC-seeded agarose constructs without TGF-beta, and that hMSCs are capable of withstanding high initial pressures that may initiate chondrogensesis faster than lower pressures. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2007 journal article

Effects of serial passaging on the adipogenic and osteogenic differentiation potential of adipose-derived human mesenchymal stem cells

TISSUE ENGINEERING, 13(6), 1291–1298.

By: M. Wall n, S. Bernacki n & E. Loboa n

MeSH headings : Adipocytes / cytology; Adipocytes / physiology; Adipogenesis / physiology; Adult; Cell Culture Techniques / methods; Cell Differentiation; Cells, Cultured; Female; Humans; Mesenchymal Stem Cells / cytology; Mesenchymal Stem Cells / physiology; Middle Aged; Osteocytes / cytology; Osteocytes / physiology; Osteogenesis / physiology; Tissue Engineering / methods
TL;DR: The results of this study indicate that adipose-derived hMSCs are capable of both adipogenic and osteogenic differentiation through 10 passages (34 population doublings) but that osteogenic differentiate may start to dominate at later passages. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2006 article

Osteogenic differentiation of human mesenchymal stem cells in collagen matrices: Effect of uniaxial cyclic tensile strain on bone morphogenetic protein (BMP-2) mRNA expression

Sumanasinghe, R. D., Bernacki, S. H., & Loboa, E. G. (2006, December). TISSUE ENGINEERING, Vol. 12, pp. 3459–3465.

By: R. Sumanasinghe n, S. Bernacki n & E. Loboa n

MeSH headings : Adult; Bone Morphogenetic Protein 2; Bone Morphogenetic Proteins / biosynthesis; Bone Morphogenetic Proteins / genetics; Bone and Bones / cytology; Bone and Bones / metabolism; Cell Differentiation / physiology; Cell Survival / physiology; Cells, Cultured; Collagen Type I; Gene Expression Regulation, Developmental / physiology; Humans; Male; Mesenchymal Stem Cells / cytology; Mesenchymal Stem Cells / metabolism; RNA, Messenger / biosynthesis; Tensile Strength; Transforming Growth Factor beta / biosynthesis; Transforming Growth Factor beta / genetics
TL;DR: This is the first report of the culture of bone marrow-derived hMSCs in 3D collagen matrices under cyclic strain, and the first demonstration that strain alone can induce osteogenic differentiation without the addition of osteogenic supplements. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

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