North Carolina Agricultural Research Service

Works Published in 2009

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Displaying all 14 works

Sorted by most recent date added to the index first, which may not be the same as publication date order.

2009 journal article

Erratum to: Characterization of two proline dipeptidases (prolidases) from the hyperthermophilic archaeon Pyrococcus horikoshii

Applied Microbiology and Biotechnology, 86(1), 393–393.

By: C. Theriot n, S. Tove n & A. Grunden n

TL;DR: Fig. 2 Activity of P. horikoshii prolidases over a pH range of 4.0–10.0 shows 100% specific activity corresponds to 2,321 U/mg for Phprol and 3,357 U/ mg for Ph1prol. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 21, 2021

2009 journal article

Sampling and analytical variability associated with the determination of aflatoxins and ochratoxin A in bulk lots of powdered ginger marketed in 1-lb bags

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 395(5), 1291–1299.

By: T. Whitaker n, M. Trucksess*, C. Weaver* & A. Slate n

author keywords: Aflatoxin; Ochratoxin A; Ginger; Sampling and analytical variability; Sampling; Foods/beverages; Quality assurance/control
MeSH headings : Aflatoxins / analysis; Food Contamination / analysis; Ginger / chemistry; Ochratoxins / analysis; Sample Size
TL;DR: Equations are derived to show the effect of increasing laboratory sample size and/or number of aliquots on reducing the variability of the test procedures used to estimate OTA and AF in powdered ginger. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2009 review

Targeting cancer with 'smart bombs': equipping plant virus nanoparticles for a 'seek and destroy' mission

[Review of ]. NANOMEDICINE, 4(5), 575–588.

By: S. Franzen n & S. Lommel n

author keywords: biodegradable; capsid; chemotherapy; colloid; nanoparticle plant virus; receptor-mediated endocytosis; reticuloendothelial system
MeSH headings : Endocytosis; Humans; Liposomes; Micelles; Nanoparticles; Neoplasms / drug therapy; Plant Viruses
TL;DR: The structural uniformity, cargo capacity, responsive behavior and ease of manufacturing of plant virus nanoparticles are unique properties that suggest they have a wider role to play in targeted therapy, and they are considered to have the most promise to address these complex phenomena. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2009 journal article

Expression of Pyrococcus furiosus Superoxide Reductase in Arabidopsis Enhances Heat Tolerance

PLANT PHYSIOLOGY, 151(2), 893–904.

By: Y. Im n, M. Ji n, A. Lee n, R. Killens n, A. Grunden n & W. Boss n

MeSH headings : Adaptation, Physiological; Arabidopsis / genetics; Arabidopsis / metabolism; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Ascorbate Peroxidases; Chlorophyll / metabolism; Gene Expression Regulation, Plant; Germination; Heat-Shock Response; Hot Temperature; Hydrogen Peroxide / metabolism; Immunoprecipitation; Oxidoreductases / genetics; Oxidoreductases / metabolism; Peroxidases / metabolism; Plant Extracts / metabolism; Plant Leaves / metabolism; Plant Roots / metabolism; Plants, Genetically Modified; Pyrococcus furiosus / enzymology; RNA, Messenger / genetics; RNA, Messenger / metabolism; Seedlings / enzymology; Seeds / metabolism
TL;DR: It is shown that P. furiosus SOR can be produced as a functional enzyme in planta and that plants producing SOR have enhanced tolerance to heat, light, and chemically induced ROS. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2009 journal article

Effects of antibiotics and oil on microbial profiles and fermentation in mixed cultures of ruminal microorganisms

JOURNAL OF DAIRY SCIENCE, 92(9), 4467–4480.

By: M. Johnson*, A. Devine n, J. Ellis, A. Grunden n & V. Fellner*

author keywords: ionophore; soybean oil; rumen microbial diversity; continuous culture
MeSH headings : Ammonia / metabolism; Animals; Anti-Bacterial Agents / pharmacology; Bacteria / classification; Bacteria / drug effects; Biodiversity; Bioreactors; Cattle; Fatty Acids, Volatile / metabolism; Female; Fermentation / drug effects; Hydrogen-Ion Concentration; In Vitro Techniques; Methane / metabolism; Rumen / microbiology; Soybean Oil / pharmacology
TL;DR: The terminal restriction fragment length polymorphism data suggest that microbial adaptation to the in vitro system in the control fermentor occurred within 4 d, and in contrast, treatments MO, OM, BO, and OB had little effect on culture fermentation despite differences in the patterns of microbial fragments. (via Semantic Scholar)
UN Sustainable Development Goal Categories
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2009 review

biotechnological applications of recombinant microbial prolidases

[Review of ]. Advances in applied microbiology, vol 68, 68, 99-.

Source: NC State University Libraries
Added: August 6, 2018

2009 journal article

Proteomics of Pyrococcus furiosus, a Hyperthermophilic Archaeon Refractory to Traditional Methods

JOURNAL OF PROTEOME RESEARCH, 8(8), 3844–3851.

By: A. Lee n, J. Sevinsky n, J. Bundy n, A. Grunden n & J. Stephenson n

author keywords: Pyrococcus furiosus; shotgun proteomics; archaea; hyperthermophile; high throughput; IPG-IEF; LC-MS/MS; archaeal proteins; microwave assisted acid hydrolysis
MeSH headings : Archaeal Proteins / analysis; Archaeal Proteins / metabolism; Hydrogen-Ion Concentration; Hydrolysis; Isoelectric Point; Microwaves; Peptide Fragments / analysis; Peptide Fragments / metabolism; Peptide Mapping; Protein Conformation; Proteome / analysis; Proteome / metabolism; Proteomics / methods; Pyrococcus furiosus / chemistry; Trypsin / metabolism
TL;DR: A simple shotgun proteomics approach for the global identification of the P. furiosus proteome using a detergent-based microwave assisted acid hydrolysis step coupled with an overnight trypsin digest to obtain peptides is developed. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2009 journal article

Influence of process parameters on growth of Clostridium ljungdahlii and Clostridium autoethanogenum on synthesis gas

ENZYME AND MICROBIAL TECHNOLOGY, 44(5), 281–288.

By: J. Cotter n, M. Chinn n & A. Grunden n

author keywords: Clostridium ljungdahlii; Clostridium autoethanaogenum; Synthesis gas fermentation; Ethanol; Acetate
TL;DR: Higher flow rates resulted in greater end product formation with no significant effect on product ratios in C. autoethanogenum fermentations, and the ethanol to acetate ratios were smaller at lower pH levels and higher flow rates. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2009 journal article

Molecular characterization of the recombinant iron-containing alcohol dehydrogenase from the hyperthermophilic Archaeon, Thermococcus strain ES1

EXTREMOPHILES, 13(2), 299–311.

By: X. Ying*, A. Grunden n, L. Nie*, M. Adams* & K. Ma*

author keywords: Iron-containing alcohol dehydrogenase; Heterologous expression; Hyperthermophile; Archaea; Thermococcus
MeSH headings : Alcohol Dehydrogenase / chemistry; Amino Acid Sequence; Archaea; Cloning, Molecular; Hydrogen-Ion Concentration; Iron / chemistry; Kinetics; Molecular Sequence Data; NADP / metabolism; Protein Structure, Tertiary; Recombinant Proteins / chemistry; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Temperature; Thermococcus / metabolism
TL;DR: The gene encoding a thermostable iron-containing alcohol dehydrogenase from Thermococcus Strain ES1 was cloned, sequenced and expressed in Escherichia coli and it was concluded that the gene encoding ES1 ADH was expressed successfully in E. coli. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2009 journal article

Ethanol and acetate production by Clostridium ljungdahlii and Clostridium autoethanogenum using resting cells

BIOPROCESS AND BIOSYSTEMS ENGINEERING, 32(3), 369–380.

By: J. Cotter n, M. Chinn n & A. Grunden n

author keywords: Clostridium ljungdahlii; Clostridium autoethanaogenum; Ethanol; Acetate; Resting cells; Non-growing cells
MeSH headings : Acetates / metabolism; Cell Culture Techniques / methods; Clostridium / chemistry; Clostridium / classification; Clostridium / growth & development; Clostridium / metabolism; Ethanol / metabolism; Hydrogen-Ion Concentration; Nitrogen / metabolism; Species Specificity
TL;DR: Clostridium autoethanogenum cultures were not induced into true resting states but did show improvement in total ethanol production as well as increased shifts in ethanol-to-acetate production ratios, and resting cell states were induced in autotrophic Clostridia ljungdahlii cultures. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2009 journal article

The Red clover necrotic mosaic virus origin of assembly is delimited to the RNA-2 trans-activator

VIROLOGY, 384(1), 169–178.

By: V. Basnayake n, T. Sit n & S. Lommel n

author keywords: Red clover necrotic mosaic virus; Virion assembly; Origin of assembly; trans-activator; Dianthovirus; Tombusviridae
MeSH headings : Base Pairing; Capsid Proteins / genetics; Genome, Viral; Open Reading Frames; Promoter Regions, Genetic; RNA, Viral / genetics; Tombusviridae / genetics; Tombusviridae / physiology; Tombusvirus / genetics; Trans-Activators / genetics; Transcription, Genetic; Trifolium / virology; Viral Proteins / genetics; Virion / genetics; Virion / physiology; Virus Replication
TL;DR: It is hypothesize that RNA-1 must be base-paired with RNA-2 at the TA to initiate co-packaging, and illustrates the critical importance of the multifunctional TA element as a key regulatory switch in the RCNMV life cycle. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2009 journal article

X-ray structure of ILL2, an auxin-conjugate amidohydrolase from Arabidopsis thaliana

PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 74(1), 61–71.

By: E. Bitto*, C. Bingman*, L. Bittova*, N. Houston n, R. Boston n, B. Fox*, G. Phillips*

author keywords: M20D peptidase family; auxin homeostasis; metalloenzyme; X-ray structure
MeSH headings : Amidohydrolases / chemistry; Animals; Arabidopsis / enzymology; Arabidopsis Proteins / chemistry; Bacterial Proteins / chemistry; Binding Sites; Crystallography, X-Ray; Exopeptidases / chemistry; Indoleacetic Acids / chemistry; Metalloproteases / chemistry; Models, Chemical; Protein Structure, Quaternary; Substrate Specificity
TL;DR: The structure of ILL2 suggests that this enzyme likely uses a catalytic mechanism that follows the paradigm established for the other enzymes of the M20 peptidase family, and a hydrophobic pocket nearby the catalytic dimetal center likely recognizes the indolyl moiety of the substrate. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2009 journal article

Sampling and Analytical Variability Associated with the Determination of Total Aflatoxins and Ochratoxin A in Powdered Ginger Sold As a Dietary Supplement in Capsules

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 57(2), 321–325.

By: M. Trucksess*, T. Whitaker*, C. Weaver*, A. Slate*, F. Giesbrecht*, J. Rader*, J. Betz*

author keywords: Sampling; analytical uncertainty; aflatoxins; ochratoxin A; ginger capsules; dietary supplements
MeSH headings : Aflatoxins / analysis; Analysis of Variance; Capsules / chemistry; Chromatography, High Pressure Liquid / methods; Chromatography, High Pressure Liquid / standards; Chromatography, High Pressure Liquid / statistics & numerical data; Consumer Product Safety; Dietary Supplements / analysis; Ginger / chemistry; Ochratoxins / analysis
TL;DR: The U.S. Food and Drug Administration is studying the need to monitor dietary supplements for mycotoxins such as total aflatoxins and ochratoxin A and the total variability associated with measuring AF and OTA in powdered ginger was partitioned into bottle-to-bottle, within bottle, and analytical variances. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2009 journal article

Maximum Potential Vegetative and Floral Production and Fruit Characteristics of Watermelon Pollenizers

HORTSCIENCE, 44(1), 59–63.

author keywords: Citrullus lanatus; triploid; seedless; diploid; pollination
TL;DR: The objective of this research was to characterize maximum potential vegetative growth, staminate and pistillate flower production over time, and measure exterior and interior fruit characteristics of pollenizer cultigens. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

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