Casey Michelle Theriot
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Works (59)
Updated: October 26th, 2024 05:02
2024 review
Bile acids impact the microbiota, host, and <i>C. difficile</i> dynamics providing insight into mechanisms of efficacy of FMTs and microbiota-focused therapeutics
[Review of ]. GUT MICROBES, 16(1).
author keywords: Clostridioides difficile; recurrent CDI; microbiota; bile acids; fecal microbiota transplantation; nuclear receptors
open access via doi.org (publisher)
Source: Web Of Science
Added: September 16, 2024
2024 article
Metagenomic, metabolomic, and lipidomic shifts associated with fecal microbiota transplantation for recurrent <i>Clostridioides difficile</i> infection
McMillan, A. S., Zhang, G., Dougherty, M. K., McGill, S. K., Gulati, A. S., Baker, E. S., & Theriot, C. M. (2024, October 8). MSPHERE.
author keywords: fecal microbiota transplant; Clostridioides difficile; lipids; acylcarnitines; bile acids; microbial conjugated bile acids; bile salt hydrolase; amino acids
Source: Web Of Science
Added: October 21, 2024
2024 article
Offline Two-Dimensional Liquid Chromatography-Mass Spectrometry for Deep Annotation of the Fecal Metabolome Following Fecal Microbiota Transplantation
Anderson, B. G., Raskind, A., Hissong, R., Dougherty, M. K., McGill, S. K., Gulati, A. S., … Evans, C. R. (2024, May 16). JOURNAL OF PROTEOME RESEARCH.
author keywords: Clostridioides difficile; C. diff; HILIC; RPLC; LC x LC; untargeted metabolomics; compound identification; bile acids; LC-MS; MS/MS
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: May 28, 2024
2023 article
Bile salt hydrolases shape the bile acid landscape and restrict Clostridioides difficile growth in the murine gut
Foley, M. H., Walker, M. E., Stewart, A. K., O'Flaherty, S., Gentry, E. C., Patel, S., … Theriot, C. M. (2023, March 13). NATURE MICROBIOLOGY, Vol. 3.
MeSH headings : Animals; Mice; Humans; Clostridioides difficile; Clostridioides; Bile Acids and Salts; Amidohydrolases; Clostridium Infections
TL;DR:
Insight is provided into the structural basis of BSH mechanisms that shape the BA landscape and promote colonization resistance against C. difficile in vitro and in pre-clinical in vivo models of CDI.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
15. Life on Land
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: April 4, 2023
2023 journal article
Intestinal bile acids provide a surmountable barrier against C. difficile TcdB-induced disease pathogenesis
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 120(19).
author keywords: toxin; host; C; difficile; pathogenesis; bile acid
MeSH headings : Humans; Mice; Animals; Bacterial Toxins; Clostridioides difficile; Bile Acids and Salts; Clostridium Infections / pathology; Intestines / pathology; Bacterial Proteins
TL;DR:
It is shown that small molecules extracted from intestinal samples from mice and humans protect against low to moderate levels of TcdB; however, at higher concentrations of toxin, inhibition is overcome and an anti-toxin approach to treat disease is provided.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 21, 2023
2023 journal article
The microbial-derived bile acid lithocholate and its epimers inhibit <i>Clostridioides difficile</i> growth and pathogenicity while sparing members of the gut microbiota
JOURNAL OF BACTERIOLOGY, 205(9).
author keywords: lithocholate; Clostridioides difficile; epimers; secondary bile acids; gut microbiota
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: April 8, 2024
2023 journal article
Using Multidimensional Separations to Distinguish Isomeric Amino Acid-Bile Acid Conjugates and Assess Their Presence and Perturbations in Model Systems
ANALYTICAL CHEMISTRY, 95(41), 15357–15366.
TL;DR:
This work analyzed 8 core bile acids synthetically conjugated with 22 proteinogenic and nonproteogenic amino acids totaling 176 MCBAs and created an in-house extended bile acid library that was applied to healthy mice dosed with antibiotics and humans having fecal microbiota transplantation (FMT) to assess the MCBA presence and changes in the gut before and after each perturbation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: October 30, 2023
2022 journal article
Bile acid distributions, sex-specificity, and prognosis in colorectal cancer
BIOLOGY OF SEX DIFFERENCES, 13(1).
MeSH headings : Female; Humans; Male; Bile Acids and Salts; Ursodeoxycholic Acid / therapeutic use; Ursodeoxycholic Acid / metabolism; Glycochenodeoxycholic Acid; Colorectal Neoplasms / metabolism; Colorectal Neoplasms / pathology; Lithocholic Acid / metabolism; Chenodeoxycholic Acid / metabolism; Colonic Neoplasms; Sex Distribution; Forkhead Transcription Factors
TL;DR:
The distribution of bile acid abundances in colon cancer patients is tumor location-, age- and sex-specific, and are linked to patient prognosis, as revealed in this study.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: November 7, 2022
2022 journal article
Prolonged oral antimicrobial administration prevents doxorubicin-induced loss of active intestinal stem cells
GUT MICROBES, 14(1).
author keywords: Microbiota; intestinal stem cells; doxorubicin; antibiotics; antimicrobials; DNA damage; germ free; injury; small intestine
MeSH headings : Administration, Oral; Animals; Anti-Bacterial Agents / administration & dosage; Anti-Bacterial Agents / pharmacology; Antineoplastic Agents / administration & dosage; Antineoplastic Agents / adverse effects; Bacteria / classification; Bacteria / drug effects; Bacteria / genetics; Bacteria / isolation & purification; Cell Survival / drug effects; Doxorubicin / administration & dosage; Doxorubicin / adverse effects; Gastrointestinal Microbiome / drug effects; Germ-Free Life; Humans; Jejunum / cytology; Jejunum / drug effects; Jejunum / microbiology; Mice; Mice, Inbred C57BL; Mucositis / microbiology; Mucositis / prevention & control; Stem Cells / cytology; Stem Cells / drug effects; Time Factors
TL;DR:
Pro-survival signaling from microbiota in AMBx-treated mice to the aISCs is suggested, and that this signaling maintains aISC retention in the face of chemotherapeutic injury.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: January 18, 2022
2022 article
Tauroursodeoxycholic Acid Inhibits Clostridioides difficile Toxin-Induced Apoptosis
Pike, C. M., Tam, J., Melnyk, R. A., & Theriot, C. M. (2022, July 7). INFECTION AND IMMUNITY.
author keywords: Clostridioides difficile; apoptosis; bile acids; tauroursodeoxycholic acid; toxin; ursodeoxycholic acid
MeSH headings : Anti-Bacterial Agents / pharmacology; Antibodies, Bacterial / pharmacology; Apoptosis; Bile Acids and Salts / pharmacology; Caco-2 Cells; Clostridioides difficile; Clostridium Infections / microbiology; Humans; Inflammation; Taurochenodeoxycholic Acid; Ursodeoxycholic Acid / pharmacology
TL;DR:
It is demonstrated that bile acid conjugation can have profound effects on C. difficile as well as the host and that conjugated and unconjugated bile acids may exert different therapeutic mechanisms against CDI.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: July 26, 2022
2022 journal article
The Stickland Reaction Precursor trans-4-Hydroxy-l-Proline Differentially Impacts the Metabolism of Clostridioides difficile and Commensal Clostridia
MSPHERE, 7(2).
author keywords: Clostridioides difficile; hydroxyproline; amino acids; Clostridia; Stickland reaction; colonization resistance; Stickland fermentation
MeSH headings : Animals; Clostridioides; Clostridioides difficile / genetics; Clostridium; Clostridium Infections / metabolism; Hydroxyproline / chemistry; Hydroxyproline / metabolism; Mice; Peptide Hydrolases; Proline / metabolism
TL;DR:
A C. difficile ΔhypD mutant mutant was constructed and found that it was modestly impaired in fitness in a mouse model of infection, and was associated with an altered microbiota when compared to mice challenged with the wild-type strain.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: July 5, 2022
2021 journal article
Clostridioides difficile exploits toxin-mediated inflammation to alter the host nutritional landscape and exclude competitors from the gut microbiota
NATURE COMMUNICATIONS, 12(1).
MeSH headings : Animals; Anti-Bacterial Agents / adverse effects; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Bacterial Toxins / genetics; Bacterial Toxins / immunology; Bacterial Toxins / metabolism; Bacteroides / drug effects; Bacteroides / metabolism; Clostridioides difficile / genetics; Clostridioides difficile / immunology; Clostridioides difficile / metabolism; Clostridium Infections / immunology; Clostridium Infections / microbiology; Clostridium Infections / pathology; Disease Models, Animal; Extracellular Matrix / metabolism; Female; Gastrointestinal Microbiome / drug effects; Gastrointestinal Microbiome / immunology; Gene Expression Regulation, Bacterial / immunology; Host-Pathogen Interactions / genetics; Host-Pathogen Interactions / immunology; Humans; Intestinal Mucosa / immunology; Intestinal Mucosa / microbiology; Intestinal Mucosa / pathology; Male; Matrix Metalloproteinases / metabolism; Mice; Nutrients / metabolism; Proteolysis; RNA, Bacterial / genetics; RNA, Bacterial / isolation & purification; RNA-Seq; Sigma Factor / genetics; Sigma Factor / immunology; Sigma Factor / metabolism; Transcriptome / immunology
TL;DR:
Insight is provided into how toxin-induced inflammation alters C. difficile metabolism, host tissue gene expression and gut microbiota, together influencing a beneficial niche for infection.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: January 20, 2021
2021 review
Contribution of Inhibitory Metabolites and Competition for Nutrients to Colonization Resistance against Clostridioides difficile by Commensal Clostridium
[Review of ]. MICROORGANISMS, 9(2).
author keywords: Clostridioides difficile; Clostridium scindens; secondary bile acids; deconjugation; dehydroxylation; epimerization; short-chain fatty acids; proline; hydroxyproline
TL;DR:
Understanding the mechanisms of colonization resistance against C. difficile is important for elucidating the mechanisms by which the pathogen is able to colonize the gut after antibiotics.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: March 15, 2021
2021 journal article
Lactobacillus bile salt hydrolase substrate specificity governs bacterial fitness and host colonization
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 118(6).
author keywords: Lactobacillus; Acidophilus; gasseri; bile salt hydrolase; bile acid
MeSH headings : Amidohydrolases / genetics; Amidohydrolases / metabolism; Ecosystem; Gastrointestinal Microbiome / genetics; Gastrointestinal Microbiome / physiology; Genetic Fitness / genetics; Host-Pathogen Interactions / genetics; Humans; Lactobacillus / enzymology; Lactobacillus / genetics; Probiotics / pharmacology; Substrate Specificity / genetics
TL;DR:
It is shown that BA type and BSH substrate preferences affect in vitro and in vivo growth of both species and that BSH enzymatic preferences and the intrinsic chemical features of various BAs determine the toxicity of these molecules during Lactobacillus growth.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: February 2, 2021
2021 journal article
Secondary bile acid ursodeoxycholic acid alters weight, the gut microbiota, and the bile acid pool in conventional mice
PLOS ONE, 16(2).
MeSH headings : Animals; Body Weight / drug effects; Cecum / microbiology; Feces / microbiology; Female; Gastrointestinal Microbiome / drug effects; Ileum / microbiology; Male; Metabolome / drug effects; Mice, Inbred C57BL; Receptors, Cytoplasmic and Nuclear / metabolism; Receptors, G-Protein-Coupled / metabolism; Ursodeoxycholic Acid / administration & dosage; Ursodeoxycholic Acid / pharmacology; Weight Loss / drug effects
TL;DR:
This study is the first to provide a comprehensive view of how exogenously administered ursodiol shapes the healthy gastrointestinal ecosystem in conventional mice and how these changes in turn modify the host physiologic response are important.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: March 22, 2021
2020 journal article
Clostridioides difficile carriage in animals and the associated changes in the host fecal microbiota
ANAEROBE, 66.
author keywords: C. difficile; C. hiranonis; Microbiome; Ribotype; Antibiotic resistance; Animal; Canine; Equine; Feline
MeSH headings : Animals; Anti-Bacterial Agents / pharmacology; Bacterial Toxins / genetics; Bacterial Toxins / metabolism; Bacterial Typing Techniques; Cats; Chlorocebus aethiops; Clostridioides difficile / classification; Clostridioides difficile / drug effects; Clostridioides difficile / physiology; Clostridium Infections / epidemiology; Clostridium Infections / microbiology; Clostridium Infections / veterinary; Coculture Techniques; Dogs; Feces / microbiology; Female; Gastrointestinal Microbiome; Horses; Hospitals, Animal; Host-Pathogen Interactions; Male; Microbial Interactions; Microbial Sensitivity Tests; North Carolina; Polymerase Chain Reaction; Prevalence; RNA, Ribosomal, 16S; Ribotyping; Risk Factors; Tertiary Healthcare; Vero Cells
TL;DR:
Experimental results showed a clear antagonistic relationship between the two strains in vitro, suggesting that commensal Clostridia might play a role in colonization resistance against C. difficile in different hosts.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: January 11, 2021
2020 journal article
Intestinal bile acids directly modulate the structure and function of C. difficile TcdB toxin
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 117(12), 6792–6800.
author keywords: C. difficile; toxin; bile acid; pathogenesis; structure
MeSH headings : Bacterial Toxins / chemistry; Bacterial Toxins / metabolism; Bile Acids and Salts / metabolism; Caco-2 Cells; Clostridioides difficile / growth & development; Clostridioides difficile / metabolism; Clostridium Infections / microbiology; HCT116 Cells; Humans; Intestines / physiology; Receptors, Cell Surface / metabolism
TL;DR:
It is shown that intestinal bile acids, which are known to play a role in C. difficile germination and outgrowth, also directly bind and inhibit TcdB toxin, the primary virulence determinant of C. diffuse disease.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: April 14, 2020
2020 journal article
Mechanisms of Colonization Resistance Against Clostridioides difficile
JOURNAL OF INFECTIOUS DISEASES, 223, S194–S200.
author keywords: bacteria; bile acids; Clostridioides difficile; microbiota; short-chain fatty acids
MeSH headings : Animals; Anti-Bacterial Agents / immunology; Anti-Bacterial Agents / metabolism; Anti-Bacterial Agents / therapeutic use; Antibiosis; Bile Acids and Salts / immunology; Bile Acids and Salts / metabolism; Clostridioides difficile / drug effects; Clostridioides difficile / growth & development; Clostridioides difficile / metabolism; Clostridium Infections / drug therapy; Clostridium Infections / immunology; Clostridium Infections / microbiology; Fatty Acids, Volatile / immunology; Fatty Acids, Volatile / metabolism; Gastrointestinal Microbiome / drug effects; Gastrointestinal Microbiome / physiology; Humans; Nutrients / immunology; Nutrients / metabolism
TL;DR:
Commensal gut microbes are critical for providing colonization resistance against C. difficile, and can be leveraged as non-antibiotic alternative therapeutics for the prevention and treatment of CDI.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: October 18, 2021
2020 article
Role of Microbiota-Derived Bile Acids in Enteric Infections
Theriot, C. M., & Petri, W. A., Jr. (2020, June 25). CELL, Vol. 181, pp. 1452–1454.
MeSH headings : Bile Acids and Salts; Cholera; Gastrointestinal Microbiome; Humans; Microbiota; Vibrio cholerae
TL;DR:
It is reported that infection by Vibrio cholerae is blocked by gut microbiome-mediated hydrolysis of bile acids, and cholera joins amebic dysentery and Clostridioides difficile colitis as enteric infections profoundly influenced by the microbiome's impact on bile acid metabolism.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: July 20, 2020
2020 journal article
Salicylanilide Analog Minimizes Relapse of Clostridioides difficile Infection in Mice
JOURNAL OF MEDICINAL CHEMISTRY, 63(13), 6898–6908.
MeSH headings : Animals; Anti-Bacterial Agents / chemistry; Anti-Bacterial Agents / pharmacokinetics; Anti-Bacterial Agents / pharmacology; Anti-Bacterial Agents / therapeutic use; Clostridioides difficile / drug effects; Clostridioides difficile / physiology; Clostridium Infections / drug therapy; Female; Male; Mice; Mice, Inbred C57BL; Recurrence; Safety; Salicylanilides / chemistry; Salicylanilides / pharmacokinetics; Salicylanilides / pharmacology; Salicylanilides / therapeutic use; Tissue Distribution
TL;DR:
A new series of known membrane-targeting compounds was synthesized and the most potent analog was selected through an in vitro inhibitory assay to evaluate its pharmacokinetic parameters and potency in a CDI mouse model, revealing reduced recurrence of CDI and disturbance of the microbiota in mice compared to standard-of-care vancomycin, thus paving the way for novel therapy that can potentially target the cell membrane of C. difficile.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: August 10, 2020
2020 journal article
Ursodeoxycholic Acid (UDCA) Mitigates the Host Inflammatory Response during Clostridioides difficile Infection by Altering Gut Bile Acids
INFECTION AND IMMUNITY, 88(6).
author keywords: C. difficile; FXR; UDCA; bile acids; inflammation; microbiome; ursodiol
MeSH headings : Animals; Bile Acids and Salts / metabolism; Biomarkers; Clostridioides difficile / drug effects; Clostridium Infections / drug therapy; Clostridium Infections / genetics; Clostridium Infections / metabolism; Clostridium Infections / microbiology; Computational Biology / methods; Dose-Response Relationship, Drug; Fibroblast Growth Factors / metabolism; Fragile X Mental Retardation Protein / metabolism; Gastrointestinal Microbiome / drug effects; Gene Expression Profiling; Host-Pathogen Interactions / drug effects; Host-Pathogen Interactions / genetics; Humans; Life Cycle Stages; Mice; Signal Transduction; Transcriptome; Ursodeoxycholic Acid / pharmacology; Ursodeoxycholic Acid / physiology
TL;DR:
Although ursodiol pretreatment did not result in a consistent decrease in the C. difficile life cycle in vivo, it was able to attenuate an overly robust inflammatory response that is detrimental to the host during CDI.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: June 15, 2020
2019 journal article
Bile salt hydrolases: Gatekeepers of bile acid metabolism and host-microbiome crosstalk in the gastrointestinal tract
PLOS PATHOGENS, 15(3).
Ed(s): L. Knoll
MeSH headings : Amidohydrolases / metabolism; Amidohydrolases / physiology; Bile Acids and Salts / metabolism; Gastrointestinal Microbiome / physiology; Gastrointestinal Tract; Host Microbial Interactions / physiology; Humans
TL;DR:
Bile acids serve as substrates for bile acid receptors (BARs) found throughout the body that control critical regulatory and metabolic processes and therefore represent an important class of bioactive molecules, and there remain gaps in knowledge about the bacterial enzymes driving their composition and modification.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: April 15, 2019
2019 journal article
Ceftiofur formulation differentially affects the intestinal drug concentration, resistance of fecal Escherichia coli, and the microbiome of steers
PLOS ONE, 14(10).
MeSH headings : Animals; Anti-Bacterial Agents / administration & dosage; Anti-Bacterial Agents / chemistry; Anti-Bacterial Agents / pharmacokinetics; Cattle; Cattle Diseases / drug therapy; Cattle Diseases / microbiology; Cephalosporins / administration & dosage; Cephalosporins / chemistry; Cephalosporins / pharmacokinetics; Drug Resistance, Bacterial; Escherichia coli / classification; Escherichia coli / drug effects; Escherichia coli / genetics; Feces / microbiology; Microbial Sensitivity Tests; Microbiota; RNA, Ribosomal, 16S / genetics
TL;DR:
CCFA leads to prolonged, low intestinal drug concentrations, and is associated with decreased E. coli concentration, an increased MIC of ceftiofur in E. Escherichia coli at specific time points, and shifts in the fecal microbiota.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: June 1, 2020
2019 review
Diversification of host bile acids by members of the gut microbiota
[Review of ]. GUT MICROBES, 11(2), 158–171.
author keywords: C. difficile; bile acids; gut microbiota; ursodeoxycholic acid (UDCA); FXR
MeSH headings : Animals; Bacteria / metabolism; Bile Acids and Salts / biosynthesis; Bile Acids and Salts / metabolism; Cholesterol / metabolism; Clostridioides difficile / pathogenicity; Gastrointestinal Microbiome / drug effects; Gastrointestinal Microbiome / physiology; Hepatocytes / metabolism; Humans; Lipid Metabolism; Microbiota / drug effects; Receptors, Cytoplasmic and Nuclear / metabolism; Ursodeoxycholic Acid / pharmacology
TL;DR:
The physiologic aspects of collaborative bile acid metabolism by the host and gut microbiota are described and the effects of ursodeoxycholic acid (UDCA) administration, a common therapeutic bile acids administration, on the gut microbiota-bile acid-host axis are discussed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: March 30, 2020
2019 article
Human fecal metabolomic profiling could inform Clostridioides difficile infection diagnosis and treatment
Theriot, C. M., & Fletcher, J. R. (2019, September 3). JOURNAL OF CLINICAL INVESTIGATION, Vol. 129, pp. 3539–3541.
MeSH headings : Clostridioides difficile; Clostridium Infections; Diarrhea; Feces; Humans; Microbiota
TL;DR:
A logistic regression model based on the fecal metabolome is used and a metabolic definition of human C. difficile infection is constructed, which could improve diagnostic accuracy and aid in the development of targeted therapeutics against this pathogen.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: September 30, 2019
2018 journal article
A Small Molecule-Screening Pipeline to Evaluate the Therapeutic Potential of 2-Aminoimidazole Molecules Against Clostridium difficile
FRONTIERS IN MICROBIOLOGY, 9.
author keywords: C. difficile; small molecules; 2-aminoimidazole; growth; toxin; sporulation
TL;DR:
A comprehensive small molecule-screening pipeline was developed to investigate how novel small molecules affect different stages of the C. difficile life cycle (growth, toxin, and sporulation) in vitro, and a library of commensal bacteria that are associated with colonization resistance against C.difficile.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2018 journal article
Beyond Structure: Defining the Function of the Gut Using Omic Approaches for Rational Design of Personalized Therapeutics
MSYSTEMS, 3(2).
author keywords: Clostridium difficile; bile acids; metabolome; microbiota; obesity; therapeutics
TL;DR:
This perspective will focus on my contribution to the microbiome field, both past and present, and where I think research in the field is headed in the near future.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2018 journal article
Dosing Regimen of Enrofloxacin Impacts Intestinal Pharmacokinetics and the Fecal Microbiota in Steers
Frontiers in Microbiology, 9.
author keywords: antimicrobial resistance; fluoroquinolone; cattle; microbiome; pharmacokinetics
TL;DR:
Both dosing regimens of enrofloxacin achieve high concentrations in the intestinal lumen, and the rapid elimination mitigates long-term impacts on fecal E. coli resistance and the microbiota.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, Crossref, NC State University Libraries, ORCID
Added: October 16, 2018
2018 journal article
MICROBIOME Gut microbiome-mediated bile acid metabolism regulates liver cancer via NKT cells
Science, 360(6391), 876-.
Source: NC State University Libraries
Added: August 6, 2018
2018 article
Restoration of short chain fatty acid and bile acid metabolism following fecal microbiota transplantation in patients with recurrent Clostridium difficile infection
Seekatz, A. M., Theriot, C. M., Rao, K., Chang, Y.-M., Freeman, A. E., Kao, J. Y., & Young, V. B. (2018, October). ANAEROBE, Vol. 53, pp. 64–73.
author keywords: Fecal microbiota transplantation; Short chain fatty acids; Bile acids; Human microbiome; Gut microbiota
MeSH headings : Adult; Aged; Bile Acids and Salts / metabolism; Clostridium Infections / therapy; Fatty Acids, Volatile / metabolism; Fecal Microbiota Transplantation; Female; Gastrointestinal Microbiome; Humans; Longitudinal Studies; Male; Metabolomics; Middle Aged; RNA, Bacterial / genetics; RNA, Ribosomal, 16S / genetics; Secondary Prevention / methods; Sequence Analysis, DNA; Young Adult
TL;DR:
Assessment of recovery of short chain fatty acids (SCFAs) in addition to bile acids alongside microbial community structure in six patients with recurrent CDI following treatment with FMT revealed variability between and within patients following FMT.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: November 26, 2018
2018 journal article
Shifts in the Gut Metabolome and Clostridium difficile Transcriptome throughout Colonization and Infection in a Mouse Model
MSPHERE, 3(2).
Contributors: J. Fletcher n, S. Erwin n , C. Lanzas n & n
author keywords: Clostridium difficile; amino acids; intestinal colonization; metabolomics; peptides; transcriptomics
MeSH headings : Amino Acids, Branched-Chain / metabolism; Animals; Anti-Bacterial Agents / administration & dosage; Carbohydrate Metabolism; Cecum / microbiology; Clostridioides difficile / metabolism; Clostridium Infections / metabolism; Clostridium Infections / microbiology; Female; Gastrointestinal Microbiome; Gene Expression Profiling; Male; Mass Spectrometry; Metabolome; Metabolomics; Mice; Mice, Inbred C57BL; Peptide Hydrolases / genetics; Proline / metabolism; Sequence Analysis, RNA; Transcriptome
TL;DR:
The gut environment after antibiotics and during the initial stages of C. difficile colonization and infection is defined and amino acids, in particular, proline and branched-chain amino acids and carbohydrates decrease in abundance over time and that C.difficile gene expression is consistent with their utilization in vivo.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018
2018 journal article
The Lactobacillus Bile Salt Hydrolase Repertoire Reveals Niche-Specific Adaptation
MSPHERE, 3(3).
Ed(s): C. Ellermeier
author keywords: Lactobacillus; bile acid; bile salt hydrolase; gastrointestinal tract; niche; penicillin V acylase; probiotic; therapeutic
MeSH headings : Adaptation, Biological; Amidohydrolases / genetics; Genetic Variation; Genotype; Lactobacillus / classification; Lactobacillus / enzymology; Lactobacillus / genetics; Penicillin Amidase / genetics; Phylogeny; Sequence Homology
TL;DR:
The BSH repertoire across 170 sequenced species revealed that BSH-encoding lactobacilli primarily adopt the vertebrate-adapted lifestyle but not the environmental or plant-associated subsets, and will guide future mechanistic studies of BSH activity and contribute to the development and selection of Lactobacillus strains with therapeutic potential.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2017 article
Inhibition of spore germination, growth, and toxin activity of clinically relevant C-difficile strains by gut microbiota derived secondary bile acids
Thanissery, R., Winston, J. A., & Theriot, C. M. (2017, June). ANAEROBE, Vol. 45, pp. 86–100.
author keywords: C. difficile; Bile acids; Gut microbiota; Metabolome; Germination; Growth; Toxin
MeSH headings : Animals; Anti-Bacterial Agents / metabolism; Antibiosis; Bacterial Toxins / metabolism; Bile Acids and Salts / metabolism; Clostridioides difficile / drug effects; Clostridioides difficile / growth & development; Gastrointestinal Microbiome; Gastrointestinal Tract / microbiology; Humans; Mice; Spores, Bacterial / drug effects; Spores, Bacterial / growth & development
TL;DR:
It is shown how clinically relevant C. difficile strains can have different responses when exposed to SBAs present in the gastrointestinal tract, and how these responses vary across all strains and ribotypes.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2017 article
Introduction to the special issue highlighting Anaerobe 2016
Cox, L. M., Theriot, C. M., & Fichorova, R. N. (2017, June). ANAEROBE, Vol. 45, pp. 1–2.
MeSH headings : Bacteria, Anaerobic / pathogenicity; Bacterial Infections / diagnosis; Bacterial Infections / epidemiology; Bacterial Infections / therapy; Biomedical Research / trends; Global Health; Microbiology / trends; Tennessee
Source: Web Of Science
Added: August 6, 2018
2017 chapter
Mathematical Modeling of the Effects of Nutrient Competition and Bile Acid Metabolism by the Gut Microbiota on Colonization Resistance Against Clostridium difficile
In Association for Women in Mathematics Series (pp. 137–161).
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Crossref, NC State University Libraries
Added: February 5, 2020
2016 journal article
Antibiotic-Induced Alterations of the Gut Microbiota Alter Secondary Bile Acid Production and Allow for Clostridium difficile Spore Germination and Outgrowth in the Large Intestine
MSphere, 1(1).
Ed(s): C. Ellermeier
author keywords: Clostridium difficile; bile acids; antibiotics; microbiota; colonization resistance
TL;DR:
It is hypothesized that the depletion of microbial members responsible for converting primary bile acids into secondary bile acid reduces resistance to Clostridium difficile colonization, suggesting that targeting growth of C.difficile will prove most important for future therapeutics and that antibiotic-related changes are organ specific.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, Crossref
Added: August 6, 2018
2016 journal article
Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, (118).
author keywords: Infection; Issue 118; Clostridium difficile; mouse model; antibiotic; colonization; cytotoxicity; histology; inflammation; Vero cells
MeSH headings : Animals; Anti-Bacterial Agents; Cefoperazone / pharmacology; Clostridioides difficile / drug effects; Disease Models, Animal; Humans; Mice; Mice, Inbred C57BL
TL;DR:
This cefoperazone mouse model of CDI proves a valuable experimental platform to assess the effects of novel therapeutics on the amelioration of clinical disease and on the restoration of colonization resistance against C. difficile.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2016 journal article
Impact of microbial derived secondary bile acids on colonization resistance against Clostridium difficile in the gastrointestinal tract
ANAEROBE, 41, 44–50.
author keywords: Clostridium difficile; Secondary bile acids; Colonization resistance; Gut microbiota; Antibiotics
MeSH headings : Animals; Anti-Bacterial Agents / pharmacology; Anti-Bacterial Agents / therapeutic use; Bile Acids and Salts / pharmacology; Bile Acids and Salts / physiology; Clostridioides difficile / drug effects; Clostridioides difficile / physiology; Disease Susceptibility; Drug Resistance, Neoplasm; Enterocolitis, Pseudomembranous / drug therapy; Enterocolitis, Pseudomembranous / microbiology; Gastrointestinal Microbiome; Gastrointestinal Tract / microbiology; Humans
TL;DR:
Rational manipulation of secondary bile acids may prove beneficial as a treatment for patients with CDI and shed light on how bile acid play a role in colonization resistance against C. difficile.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2016 journal article
Metabolic Model-Based Integration of Microbiome Taxonomic and Metabolomic Profiles Elucidates Mechanistic Links between Ecological and Metabolic Variation
MSystems, 1(1).
Ed(s): L. Sanchez
author keywords: microbiome; multi-omic; metabolic modeling; community composition; metabolomics
TL;DR:
A surprisingly large proportion of metabolite variation in the vaginal microbiome can be predicted based on species composition (including dramatic shifts associated with disease), identify putative mechanisms underlying these predictions, and evaluate the roles of individual bacterial species and genes.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
15. Life on Land
(OpenAlex)
Sources: Web Of Science, Crossref
Added: August 6, 2018
2015 journal article
Fecal Microbiota Transplantation Eliminates Clostridium difficile in a Murine Model of Relapsing Disease
Infection and Immunity, 83(10), 3838–3846.
Ed(s): B. McCormick
MeSH headings : Animals; Bacteria / classification; Bacteria / genetics; Bacteria / isolation & purification; Chronic Disease / therapy; Clostridioides difficile / physiology; Clostridium Infections / microbiology; Clostridium Infections / therapy; Fecal Microbiota Transplantation; Feces / microbiology; Female; Humans; Male; Mice; Mice, Inbred C57BL; Microbiota; Recurrence
TL;DR:
It is suggested that full recovery of colonization resistance against C. difficile requires the restoration of a specific community structure.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, Crossref
Added: August 6, 2018
2015 journal article
Interactions Between the Gastrointestinal Microbiome and Clostridium difficile
Annual Review of Microbiology, 69(1), 445–461.
author keywords: gut microbiota; Clostridium difficile; antibiotics; colonization resistance; bacterial metabolism; bile acids
MeSH headings : Animals; Anti-Bacterial Agents / administration & dosage; Clostridioides difficile / metabolism; Clostridium Infections / microbiology; Fermentation; Gastrointestinal Microbiome / drug effects; Humans; Immune Evasion; Microbial Interactions; Microbiota / drug effects
TL;DR:
New bacterial therapies to restore changes in bacteria-driven intestinal metabolism following antibiotics will have important applications for treatment and prevention of C. difficile infection.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, Crossref
Added: August 6, 2018
2014 journal article
Alteration of the Murine Gastrointestinal Microbiota by Tigecycline Leads to Increased Susceptibility to Clostridium difficile Infection
Antimicrobial Agents and Chemotherapy, 58(5), 2767–2774.
MeSH headings : Animals; Clostridioides difficile / drug effects; Clostridioides difficile / pathogenicity; Clostridium Infections / drug therapy; Female; Gastrointestinal Tract / microbiology; Male; Mice; Mice, Inbred C57BL; Microbiota / drug effects; Minocycline / analogs & derivatives; Minocycline / therapeutic use; Tigecycline
TL;DR:
The results indicate that microbiotic dynamics are key in the development of CDI, and a better understanding of these dynamics may lead to better strategies to prevent and treat this disease.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: Crossref
Added: July 28, 2019
2014 chapter
Antibiotic-Associated Diarrhea
In Encyclopedia of Metagenomics (pp. 1–7).
Source: Crossref
Added: July 27, 2019
2014 journal article
Antibiotic-induced shifts in the mouse gut microbiome and metabolome increase susceptibility to Clostridium difficile infection
Nature Communications, 5(1).
MeSH headings : Animals; Anti-Bacterial Agents / pharmacology; Clostridioides difficile / drug effects; Clostridioides difficile / growth & development; Clostridioides difficile / physiology; Clostridium Infections / metabolism; Clostridium Infections / microbiology; Disease Susceptibility / metabolism; Disease Susceptibility / microbiology; Female; Gastrointestinal Tract / microbiology; Male; Metabolome / drug effects; Metabolomics; Mice, Inbred C57BL; Microbiota / drug effects
TL;DR:
The authors show that antibiotic-induced shifts in the mouse gut microbiome are correlated with changes in levels of certain metabolites that C. difficile can use for germination and growth.
(via Semantic Scholar)
Source: Crossref
Added: July 28, 2019
2014 journal article
Clostridium difficile-induced colitis in mice is independent of leukotrienes
Anaerobe, 30, 90–98.
author keywords: Clostridium difficile; Leukotriene; Eicosanoid; Nosocomial infection
MeSH headings : Animals; Clostridioides difficile / growth & development; Clostridioides difficile / immunology; Clostridium Infections / microbiology; Clostridium Infections / pathology; Colitis / microbiology; Colitis / pathology; Disease Models, Animal; Female; Histocytochemistry; Leukotrienes / metabolism; Mice, Inbred C57BL
TL;DR:
Results do not support a role for LTs in modulating host susceptibility to CDI in mice, and CDI induced a burst of cytokines in the intestine of infected mice in a LT-independent manner.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: Crossref
Added: July 28, 2019
2014 journal article
Dynamics and Establishment of Clostridium difficile Infection in the Murine Gastrointestinal Tract
Infection and Immunity, 83(3), 934–941.
Ed(s): B. McCormick
MeSH headings : Animals; Anti-Bacterial Agents / adverse effects; Bile Acids and Salts / chemistry; Clostridioides difficile / growth & development; Clostridioides difficile / metabolism; Clostridioides difficile / pathogenicity; Clostridium Infections / etiology; Clostridium Infections / microbiology; Clostridium Infections / mortality; Clostridium Infections / pathology; Colitis / etiology; Colitis / microbiology; Colitis / mortality; Colitis / pathology; Disease Progression; Enterotoxins / biosynthesis; Enterotoxins / metabolism; Feces / microbiology; Female; Gastrointestinal Tract / drug effects; Gastrointestinal Tract / microbiology; Gastrointestinal Tract / pathology; Male; Mice; Mice, Inbred C57BL; Microbiota / drug effects; Spores, Bacterial / growth & development; Spores, Bacterial / metabolism; Spores, Bacterial / pathogenicity; Survival Analysis; Time Factors
TL;DR:
An integrated understanding of the timing and location of the events surrounding C. difficile colonization is provided and potential targets for the development of new therapeutic strategies are identified.
(via Semantic Scholar)
Source: Crossref
Added: July 28, 2019
2014 journal article
Effects of Tigecycline and Vancomycin Administration on Established Clostridium difficile Infection
Antimicrobial Agents and Chemotherapy, 59(3), 1596–1604.
MeSH headings : Animals; Anti-Bacterial Agents / pharmacology; Cecum / microbiology; Clostridioides difficile / drug effects; Colon / microbiology; Enterocolitis, Pseudomembranous / drug therapy; Enterocolitis, Pseudomembranous / microbiology; Feces / microbiology; Female; Male; Mice; Mice, Inbred C57BL; Minocycline / analogs & derivatives; Minocycline / pharmacology; Tigecycline; Vancomycin / pharmacology
TL;DR:
In vitro growth studies confirmed that subinhibitory concentrations of tigecycline were able to suppress toxin activity and spore formation of C. difficile, whereas vancomycin did not.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: Crossref
Added: July 27, 2019
2014 journal article
Interleukin-22 and CD160 play additive roles in the host mucosal response to Clostridium difficile infection in mice
Immunology, 144(4), 587–597.
author keywords: Clostridium difficile; CD160; interleukin-22; pSTAT3; RegIII
MeSH headings : Animals; Anti-Bacterial Agents; Antibodies / pharmacology; Antigens, CD / genetics; Antigens, CD / immunology; Antigens, CD / metabolism; Clostridioides difficile / immunology; Clostridioides difficile / pathogenicity; Disease Models, Animal; Enterocolitis, Pseudomembranous / genetics; Enterocolitis, Pseudomembranous / immunology; Enterocolitis, Pseudomembranous / metabolism; Enterocolitis, Pseudomembranous / microbiology; Enterocolitis, Pseudomembranous / prevention & control; GPI-Linked Proteins / antagonists & inhibitors; GPI-Linked Proteins / genetics; GPI-Linked Proteins / immunology; GPI-Linked Proteins / metabolism; Gene Expression Regulation; Immunity, Mucosal / drug effects; Interleukins / antagonists & inhibitors; Interleukins / genetics; Interleukins / immunology; Interleukins / metabolism; Intestinal Mucosa / drug effects; Intestinal Mucosa / immunology; Intestinal Mucosa / metabolism; Intestinal Mucosa / microbiology; Male; Mice, Inbred C57BL; Neutrophil Infiltration; Phosphorylation; Receptors, Immunologic / antagonists & inhibitors; Receptors, Immunologic / genetics; Receptors, Immunologic / immunology; Receptors, Immunologic / metabolism; STAT3 Transcription Factor / immunology; STAT3 Transcription Factor / metabolism; Signal Transduction; Time Factors
TL;DR:
These data demonstrate that IL‐22 and CD160 are together responsible for a significant fraction of the colonic STAT3 phosphorylation in C. difficile infection and underscore the additive effects of IL‐ 22 andCD160 in mediating both the pro‐inflammatory and pro‐survival aspects of the host mucosal response in this infection.
(via Semantic Scholar)
Source: Crossref
Added: September 13, 2019
2013 journal article
Acute infection of mice with Clostridium difficile leads to eIF2α phosphorylation and pro-survival signalling as part of the mucosal inflammatory response
Immunology, 140(1), 111–122.
author keywords: Clostridium difficile; eIF2 alpha; interleukin-22; pSTAT3; RegIII gamma
MeSH headings : Acute Disease; Animals; Antimicrobial Cationic Peptides / genetics; Chemokines / genetics; Clostridioides difficile / immunology; Clostridioides difficile / pathogenicity; Cytokines / genetics; Enterocolitis, Pseudomembranous / genetics; Enterocolitis, Pseudomembranous / immunology; Enterocolitis, Pseudomembranous / metabolism; Eukaryotic Initiation Factor-2 / metabolism; Immunity, Innate; Immunity, Mucosal; Inflammation Mediators / metabolism; Interleukins / genetics; Intestinal Mucosa / immunology; Male; Mice; Mice, Inbred C57BL; Phosphorylation; Signal Transduction; Transcriptome; Unfolded Protein Response
TL;DR:
Data underscore the local, innate, pro‐inflammatory nature of the response to C. difficile and highlight eIF2α phosphorylation and the interleukin‐22–pSTAT3–RegIIIγ axis as two of the pathways that could be used to contain and counteract the damage inflicted on the intestinal epithelium.
(via Semantic Scholar)
Source: Crossref
Added: September 13, 2019
2013 journal article
Microbial and metabolic interactions between the gastrointestinal tract and Clostridium difficile infection
Gut Microbes, 5(1), 86–95.
author keywords: Clostridium difficile; antibiotics; gut microbiota; gut metabolome; colonization resistance
MeSH headings : Animals; Anti-Bacterial Agents / pharmacology; Clostridioides difficile / drug effects; Clostridioides difficile / growth & development; Clostridioides difficile / metabolism; Clostridium Infections / drug therapy; Clostridium Infections / metabolism; Clostridium Infections / microbiology; Gastrointestinal Tract / metabolism; Gastrointestinal Tract / microbiology; Humans; Microbiota / drug effects
TL;DR:
How antibiotics alter the structure of the gut microbiota and how this impacts bacterial metabolism in the gut is discussed and the chemical requirements for C. difficile germination, growth, toxin production and sporulation are explored.
(via Semantic Scholar)
Source: Crossref
Added: September 13, 2019
2013 journal article
The Complete Campylobacter jejuni Transcriptome during Colonization of a Natural Host Determined by RNAseq
PLoS ONE, 8(8), e73586.
Ed(s): D. Rasko
MeSH headings : Animals; Campylobacter jejuni / genetics; Chickens / microbiology; Gene Expression Profiling; Genes, Bacterial; Reverse Transcriptase Polymerase Chain Reaction; Sequence Analysis, RNA; Transcriptome
TL;DR:
Over 250 genes differentially expressed in vivo in addition to numerous putative regulatory RNAs, including trans-acting non-coding RNAs and anti-sense transcripts are identified, highlighting the power and value of the RNAseq approach.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land
(OpenAlex)
Source: Crossref
Added: July 28, 2019
2011 journal article
Cefoperazone-treated mice as an experimental platform to assess differential virulence of Clostridium difficile strains
Gut Microbes, 2(6), 326–334.
MeSH headings : Animals; Blood Cell Count; Cecum / microbiology; Cecum / pathology; Cefoperazone / administration & dosage; Cefoperazone / pharmacology; Chlorocebus aethiops; Clostridioides difficile / pathogenicity; Colon / microbiology; Colon / pathology; Edema / microbiology; Edema / pathology; Enterocolitis, Pseudomembranous / drug therapy; Enterocolitis, Pseudomembranous / microbiology; Enterocolitis, Pseudomembranous / pathology; Female; Intestinal Mucosa / microbiology; Intestinal Mucosa / pathology; Male; Mice; Mice, Inbred C57BL; Models, Animal; Neutrophils / metabolism; Vero Cells; Virulence; Weight Loss
TL;DR:
Cefoperazone treated mice represent a useful model of C. difficile infection that will help to better understand the pathogenesis and virulence of this re-emerging pathogen.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: Crossref
Added: January 24, 2021
2011 journal article
Improving the Catalytic Activity of Hyperthermophilic Pyrococcus horikoshii Prolidase for Detoxification of Organophosphorus Nerve Agents over a Broad Range of Temperatures
Archaea, 2011, 1–9.
Ed(s): B. C.M. & S. Shah
MeSH headings : Biotransformation; Chemical Warfare Agents / metabolism; Dipeptidases / genetics; Dipeptidases / metabolism; Enzyme Stability; Hydrogen-Ion Concentration; Mutant Proteins / genetics; Mutant Proteins / metabolism; Mutation; Organophosphates / metabolism; Pyrococcus horikoshii / chemistry; Pyrococcus horikoshii / enzymology; Pyrococcus horikoshii / metabolism; Sarin / metabolism; Soman / metabolism; Temperature
TL;DR:
Four Ph1prol mutants were isolated which had greater thermostability and improved activity over a broader range of temperatures against Xaa-Pro dipeptides and OP nerve agents compared to wild type Pyrococcus prolidases.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Crossref, Web Of Science, NC State University Libraries
Added: August 6, 2018
2011 journal article
The interplay between microbiome dynamics and pathogen dynamics in a murine model of Clostridium difficile Infection
Gut Microbes, 2(3), 145–158.
author keywords: Clostridium difficile; colonization resistance; microbial ecology; antibiotic-associated diarrhea; C. difficile infection
MeSH headings : Animals; Anti-Bacterial Agents / pharmacology; Bacteria / classification; Bacteria / drug effects; Bacteria / genetics; Bacteria / isolation & purification; Clostridioides difficile / drug effects; Clostridioides difficile / genetics; Clostridioides difficile / isolation & purification; Clostridioides difficile / physiology; Clostridium Infections / microbiology; Colitis / microbiology; Disease Models, Animal; Gastrointestinal Tract / drug effects; Gastrointestinal Tract / microbiology; Humans; Metagenome / drug effects; Mice; Mice, Inbred C57BL
TL;DR:
The severity of colitis that arises in this system reflects the interplay between the expansion of C. difficile in the gut community and the ecologic dynamics of the indigenous microbes as it recovers from antibiotic perturbation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: Crossref
Added: January 24, 2021
2010 journal article
Hydrolysis of organophosphorus compounds by microbial enzymes
Applied Microbiology and Biotechnology, 89(1), 35–43.
author keywords: Organophosphorus compound; OP nerve agent; Pesticide; OPAA; OPH; Phosphotriesterase; Prolidase
MeSH headings : Aryldialkylphosphatase / chemistry; Aryldialkylphosphatase / genetics; Aryldialkylphosphatase / metabolism; Bacteria / chemistry; Bacteria / enzymology; Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Biocatalysis; Hydrolysis; Kinetics; Organophosphorus Compounds / chemistry; Substrate Specificity
TL;DR:
An update of what is experimentally known about OPH and OPAA to include their structures, substrate specificity, and catalytic properties is provided.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 6, 2018
2010 journal article
Improving the catalytic activity of hyperthermophilic Pyrococcus prolidases for detoxification of organophosphorus nerve agents over a broad range of temperatures
Applied Microbiology and Biotechnology, 87(5), 1715–1726.
author keywords: Prolidase; Pyrococcus furiosus; OP nerve agents; Mutagenesis; Directed evolution
MeSH headings : Amino Acid Substitution / genetics; Archaeal Proteins / chemistry; Archaeal Proteins / genetics; Archaeal Proteins / isolation & purification; Archaeal Proteins / metabolism; Chemical Warfare Agents / metabolism; Culture Media / chemistry; Dipeptidases / chemistry; Dipeptidases / genetics; Dipeptidases / isolation & purification; Dipeptidases / metabolism; Enzyme Stability; Escherichia coli / genetics; Models, Molecular; Mutagenesis; Organophosphorus Compounds / metabolism; Pesticides / metabolism; Plasmids; Polymerase Chain Reaction / methods; Protein Stability; Protein Structure, Tertiary; Pyrococcus furiosus / enzymology; Pyrococcus furiosus / genetics; Sequence Deletion; Temperature
TL;DR:
To obtain a better enzyme for OP nerve agent decontamination and to investigate structural factors that influence protein thermostability and thermoactivity, randomly mutated P. furiosus prolidase mutants with improved activity over a broader range of temperatures were isolated.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 6, 2018
2009 journal article
Characterization of two proline dipeptidases (prolidases) from the hyperthermophilic archaeon Pyrococcus horikoshii
Applied Microbiology and Biotechnology, 86(1), 177–188.
author keywords: Prolidase; Pyrococcus horikoshii; Hyperthermophile; Metalloenzyme; Cobalt enzyme
MeSH headings : Amino Acid Sequence; Archaeal Proteins / chemistry; Archaeal Proteins / genetics; Archaeal Proteins / metabolism; Biotechnology / methods; Catalytic Domain; Cobalt / metabolism; Dipeptidases / chemistry; Dipeptidases / genetics; Dipeptidases / metabolism; Enzyme Stability; Hot Temperature; Hydrogen-Ion Concentration; Molecular Sequence Data; Pyrococcus horikoshii / enzymology; Pyrococcus horikoshii / genetics; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Substrate Specificity
TL;DR:
Biochemical characterization of two thermostable prolidases identified in the genome of Pyrococcus horikoshii shows they have higher catalytic activities over a broader pH range, higher affinity for metal and are more stable compared to P. furiosus prolidase.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 6, 2018
2009 journal article
Erratum to: Characterization of two proline dipeptidases (prolidases) from the hyperthermophilic archaeon Pyrococcus horikoshii
Applied Microbiology and Biotechnology, 86(1), 393–393.
TL;DR:
Fig. 2 Activity of P. horikoshii prolidases over a pH range of 4.0–10.0 shows 100% specific activity corresponds to 2,321 U/mg for Phprol and 3,357 U/ mg for Ph1prol.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 21, 2021
2009 review
biotechnological applications of recombinant microbial prolidases
[Review of ]. Advances in applied microbiology, vol 68, 68, 99-.
Source: NC State University Libraries
Added: August 6, 2018
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