Works Published in 2000
Displaying all 7 works
Sorted by most recent date added to the index first, which may not be the same as publication date order.
2000 journal article
Gene targeting in domestic species: A new beginning
Transgenic Res, 9(4/5), 261–262.
MeSH headings : Animals; Animals, Domestic / genetics; Gene Targeting / methods; Gene Targeting / veterinary
TL;DR:
The prospects and technical challenges for introducing targeted changes into the germline by this route, and the types of application for which this new technology will be used, are discussed.
(via Semantic Scholar)
Source: Web Of Science
Added: October 29, 2021
2000 journal article
Three little pigs worth the huff and puff?
Nature Biotechnology, 18(11), 1144–1145.
MeSH headings : Animals; Cell Nucleus / metabolism; Cloning, Organism; Culture Techniques / methods; Ovum / metabolism; Swine; Transplantation, Heterologous
Source: Crossref
Added: July 27, 2019
2000 journal article
Pathologic findings and association of Mycobacterium bovis infection with the bovine NRAMP1 gene in cattle from herds with naturally occurring tuberculosis
AMERICAN JOURNAL OF VETERINARY RESEARCH, 61(9), 1140–1144.
MeSH headings : Alleles; Animals; Carrier Proteins / genetics; Cation Transport Proteins; Cattle / genetics; Genetic Predisposition to Disease / genetics; Genotype; Immunity, Innate / genetics; Membrane Proteins / genetics; Mycobacterium bovis / isolation & purification; Polymorphism, Restriction Fragment Length; Polymorphism, Single-Stranded Conformational; Tuberculosis, Bovine / genetics; Tuberculosis, Bovine / pathology
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2000 journal article
Isolation and genetic transformation of primordial germ cell (PGC)-derived cells from cattle, goats, rabbits and rats
ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 13(5), 587–594.
author keywords: primordial germ cell; embryonic germ cell; alkaline phosphatase staining; embryoid body; genetic transformation; green fluorescent protein
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2000 journal article
Effects of protease inhibitors and antioxidants on in vitro survival of porcine primordial germ cells
BIOLOGY OF REPRODUCTION, 63(3), 887–897.
author keywords: apoptosis; conceptus; cytokines; growth factors
MeSH headings : Acetylcysteine / administration & dosage; Acetylcysteine / pharmacology; Animals; Antioxidants / pharmacology; Apoptosis / drug effects; Cell Survival / drug effects; Cells, Cultured; DNA Fragmentation; Dose-Response Relationship, Drug; Fetus / cytology; Germ Cells / drug effects; Germ Cells / physiology; In Situ Nick-End Labeling; Microscopy, Electron; Protease Inhibitors / pharmacology; Swine; alpha-Macroglobulins / administration & dosage; alpha-Macroglobulins / pharmacology
TL;DR:
Results indicate that the addition of α2-macroglobulin and antioxidants can increase the number of PGCs in vitro by suppressing apoptosis.
(via Semantic Scholar)
academic.oup.com (publisher PDF)
Source: Web Of Science
Added: August 6, 2018
2000 journal article
Effects of growth factors and feeder cells on porcine primordial germ cells in vitro
Cloning and Stem Cells, 2, 197–206.
MeSH headings : Alkaline Phosphatase / metabolism; Animals; Animals, Genetically Modified; COS Cells; Cell Culture Techniques; Cell Differentiation; Cell Membrane / metabolism; Chlorocebus aethiops; Culture Media / pharmacology; Embryo Culture Techniques; Embryo, Mammalian / cytology; Fibroblast Growth Factor 2 / metabolism; Fibroblasts / metabolism; Germ Cells / cytology; Germ Cells / metabolism; Growth Substances; Interleukin-6 / metabolism; Leukemia Inhibitory Factor; Lewis X Antigen / metabolism; Stem Cell Factor / metabolism; Stem Cells / cytology; Swine
TL;DR:
A combination of growth factors and proper feeder cells are critical for the establishment of undifferentiated porcine EG cells.
(via Semantic Scholar)
Source: NC State University Libraries
Added: August 6, 2018
2000 journal article
A rapid and sensitive reporter gene that uses green fluorescent protein expression to detect chemicals with estrogenic activity
TOXICOLOGICAL SCIENCES, 55(1), 69–77.
author keywords: cellular steroid receptors; endocrine disruption; xenobiotics; transfection; MCF7-ERE cells; estrogenic activity
MeSH headings : Cell Division / drug effects; Cell Line; Drug Evaluation, Preclinical; Estradiol / pharmacology; Estradiol Congeners / pharmacology; Eukaryotic Cells / drug effects; Genes, Reporter / drug effects; Genes, Reporter / genetics; Green Fluorescent Proteins; Humans; Luminescent Proteins / biosynthesis; Luminescent Proteins / genetics; Phosphoglycerate Kinase / metabolism; Plasmids / genetics; Receptors, Estrogen / drug effects; Receptors, Estrogen / genetics; Receptors, Estrogen / metabolism; Serum Albumin, Bovine / chemistry; Tamoxifen / analogs & derivatives; Tamoxifen / pharmacology; Transfection
TL;DR:
The ERE-PGK-GFP reporter gene system is capable of rapid GFP expression in the presence of low concentrations of E2, and of quantifying estrogenicity of chemicals compared with a standard curve of the natural ligand, 17beta-estradiol.
(via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018
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