Translational Predictive Biology

Works Published in 2002

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2002 journal article

Inhibition of apoptosis in serum starved porcine embryonic fibroblasts

MOLECULAR REPRODUCTION AND DEVELOPMENT, 62(1), 106–112.

By: C. Lee * & J. Piedrahita*

author keywords: cell synchronization; antioxidants; G(0)/G(1)
MeSH headings : Acetylcysteine / pharmacology; Animals; Antioxidants / pharmacology; Apoptosis; Cells, Cultured; Culture Media, Serum-Free; Female; Fibroblasts / cytology; Fibroblasts / drug effects; Free Radical Scavengers / pharmacology; Protease Inhibitors / pharmacology; Swine; alpha-Macroglobulins / pharmacology
TL;DR: The addition of MAC and antioxidants during serum starvation of PEF reduces apoptosis of quiescent fibroblasts and may contribute to increasing the efficiency of nuclear transplantation by improving the quality of donor nuclei. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2002 journal article

Effects of follicular size of cytoplast donor on the efficiency of cloning in cattle

MOLECULAR REPRODUCTION AND DEVELOPMENT, 61(3), 317–326.

By: J. Piedrahita*, D. Wells*, A. Miller*, J. Oliver*, M. Berg*, A. Peterson*, H. Tervit*

author keywords: embryo development; nuclear transfer; oocyte quality
MeSH headings : Animals; Blastocyst / cytology; Body Weight; Cattle; Cell Nucleus / physiology; Cloning, Organism / methods; Culture Techniques; Cytoplasm / physiology; Cytoplasm / transplantation; Embryo Transfer; Embryo, Mammalian / cytology; Embryo, Mammalian / embryology; Embryonic and Fetal Development; Female; Fertilization in Vitro; Microsatellite Repeats / genetics; Nuclear Transfer Techniques; Oocytes / cytology; Oocytes / growth & development; Oocytes / transplantation; Ovarian Follicle / anatomy & histology; Ovarian Follicle / cytology; Ovarian Follicle / transplantation; Pregnancy; Ultrasonography, Prenatal
TL;DR: Results indicate that cytoplasts from follicles of 1–3 and 6–12 mm in diameter are equally developmentally competent when used in a nuclear transfer procedure. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2002 journal article

A highly efficient method for porcine cloning by nuclear transfer using in vitro-matured oocytes

Cloning and Stem Cells, 2, 105–112.

By: S. Walker*, T. Shin *, G. Zaunbrecher*, J. Romano*, G. Johnson*, F. Bazer*, J. Piedrahita*

MeSH headings : Animals; Cloning, Organism / methods; Culture Techniques; Embryo Transfer; Female; Microsatellite Repeats / genetics; Nuclear Transfer Techniques; Oocytes / cytology; Pregnancy; Sex Ratio; Swine / genetics
TL;DR: A simple nuclear transfer protocol, utilizing commercially available in vitro-matured oocytes, that results in greater than 5% overall cloning efficiency is reported, and should facilitate the broad utilization of porcine cloning technology in transgenic and nontransgenic applications. (via Semantic Scholar)
Source: NC State University Libraries
Added: August 6, 2018

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