Works Published in 2001
Displaying works 21 - 23 of 23 in total
Sorted by most recent date added to the index first, which may not be the same as publication date order.
2001 journal article
Leaky Lactococcus cultures that externalize enzymes and antigens independently of culture lysis and secretion and export pathways
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 67(1), 251–259.
MeSH headings : Antigens, Bacterial / metabolism; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Bacteriophages / enzymology; Bacteriophages / genetics; Bacteriophages / physiology; Culture Media; Genetic Engineering; Lactococcus lactis / enzymology; Lactococcus lactis / genetics; Lactococcus lactis / growth & development; Lactococcus lactis / metabolism; Lysogeny; N-Acetylmuramoyl-L-alanine Amidase; Viral Proteins / genetics; Viral Proteins / metabolism; Virus Integration; beta-Galactosidase / metabolism
TL;DR:
A novel system that leaks β-galactosidase (β-gal) without a requirement for secretion or export signals was developed in lactococcus lactis by controlled expression of integrated phage holin and lysin cassettes, pointing toward the development of bacterial strains able to efficiently release relevant proteins and enzymes outside the cell in the absence of known secretion and export signals.
(via Semantic Scholar)
aem.asm.org (publisher PDF)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2001 journal article
Identification and cloning of gusA, encoding a new beta-glucuronidase from Lactobacillus gasseri ADH
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 67(3), 1253–1261.
MeSH headings : Amino Acid Sequence; Cloning, Molecular; DNA, Bacterial / genetics; Escherichia coli / enzymology; Escherichia coli / genetics; Glucuronidase / chemistry; Glucuronidase / genetics; Glucuronidase / metabolism; Lactobacillus / enzymology; Lactobacillus / genetics; Molecular Sequence Data; Open Reading Frames; Sequence Analysis, DNA
TL;DR:
This is the first report of a β-glucuronidase gene cloned from a bacterial source other than Escherichia coli, and preliminary characterization of the GusA protein from crude cell extracts revealed that the enzyme was active across an acidic pH range and a broad temperature range.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2001 journal article
Aroma-active components of nonfat dry milk
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 49(6), 2948–2953.
author keywords: nonfat dry milk; AEDA; FD factor; gas chromatography-olfactometry; gas chromatography-mass spectrometry
MeSH headings : Animals; Chromatography, Gas; Gas Chromatography-Mass Spectrometry; Hot Temperature; Milk / chemistry; Odorants / analysis; Taste
TL;DR:
Application of aroma extract dilution analysis (AEDA) on the volatile components of low, medium, and high-heat-treated nonfat dry milks (NDM) revealed aroma-active compounds in the log(3) flavor dilution) factor range of 1 to 6.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
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