Works Published in 2016
Displaying works 1 - 20 of 42 in total
Sorted by most recent date added to the index first, which may not be the same as publication date order.
2016 journal article
Prediction and Validation of Native and Engineered Cas9 Guide Sequences
Cold Spring Harbor Protocols, 2016(7), pdb.prot086785.
MeSH headings : Bacterial Proteins / metabolism; CRISPR-Associated Protein 9; Endonucleases / metabolism; Gene Editing / methods; RNA, Guide, Kinetoplastida / genetics; RNA, Guide, Kinetoplastida / metabolism; Streptococcus pyogenes / enzymology
TL;DR:
How to predict tracrRNA sequences and suggest a method for designing single guide RNAs containing only critical structural modules are discussed, which is crucial for determining potential targets for Cas9.
(via Semantic Scholar)
cshprotocols.cshlp.org (publisher PDF)
Source: ORCID
Added: January 2, 2020
2016 journal article
CRISPR-Based Typing and Next-Generation Tracking Technologies
Annual Review of Food Science and Technology, 7(1), 395–411.
author keywords: CRISPR; cas; typing; epidemiology
MeSH headings : Bacteria / classification; Bacteria / genetics; CRISPR-Cas Systems / genetics; Clustered Regularly Interspaced Short Palindromic Repeats / genetics; Clustered Regularly Interspaced Short Palindromic Repeats / physiology; Genome, Bacterial / genetics; Genotyping Techniques / methods; Genotyping Techniques / trends; Lactobacillus / classification; Lactobacillus / genetics; Salmonella / classification; Salmonella / genetics; Streptococcus thermophilus / classification; Streptococcus thermophilus / genetics
TL;DR:
Emerging CRISPR-based typing methods open new avenues for high-resolution typing of a broad range of bacteria and constitute a practical means for rapid tracking of a diversity of food-associated microbes.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: ORCID
Added: January 2, 2020
2016 journal article
Guide RNAs: A Glimpse at the Sequences that Drive CRISPR–Cas Systems
Cold Spring Harbor Protocols, 2016(7), pdb.top090902.
MeSH headings : CRISPR-Cas Systems; Gene Editing / methods; RNA, Guide, Kinetoplastida / genetics; RNA, Guide, Kinetoplastida / metabolism; Streptococcus pyogenes / enzymology
TL;DR:
The biological significance of Type II CRISPR-Cas elements, including the tracrRNA, crRNA, Cas9, and protospacer-adjacent motif (PAM), are discussed, and the native function of these elements is looked at to understand how they can be engineered, enhanced, and optimized for genome editing applications.
(via Semantic Scholar)
Source: ORCID
Added: January 2, 2020
2016 journal article
Development of a Transportation System Simulation Manual Framework From Theory to Practice
TRANSPORTATION RESEARCH RECORD, (2561), 45–52.
TL;DR:
A possible structure for a transportation system simulation manual that would cover the limitations and gaps in the existing literature is proposed and would consist of five volumes: concepts, model building, verification and validation, results analysis, and case studies and supplementary materials.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
11. Sustainable Cities and Communities
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2016 journal article
Genotyping by PCR and High-Throughput Sequencing of Commercial Probiotic Products Reveals Composition Biases
FRONTIERS IN MICROBIOLOGY, 7.
author keywords: probiotics; labeling; testing and assessment; Lactobacillus; Bifidobacterium; multiplex PCR; taxonomy; high-throughput nucleotide sequencing
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2016 journal article
CRISPR Diversity and Microevolution in Clostridium difficile
GENOME BIOLOGY AND EVOLUTION, 8(9), 2841–2855.
author keywords: CRISPR-Cas; CRISPR phylogeny; CRISPR typing; BI/NAP1/RT027/ST1Type-IB
MeSH headings : CRISPR-Cas Systems; Clostridioides difficile / classification; Clostridioides difficile / genetics; Clustered Regularly Interspaced Short Palindromic Repeats; Evolution, Molecular; Phylogeny; Polymorphism, Genetic
TL;DR:
Overall, CRISPR-Cas sequences constitute a valuable basis for genotyping of C. difficile isolates, provide insights into the micro-evolutionary events that occur between closely related strains, and reflect the evolutionary trajectory of these genomes.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2016 journal article
Multivalent Chromosomal Expression of the Clostridium botulinum Serotype A Neurotoxin Heavy-Chain Antigen and the Bacillus anthracis Protective Antigen in Lactobacillus acidophilus
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 82(20), 6091–6101.
MeSH headings : Anthrax / microbiology; Anthrax / prevention & control; Bacillus anthracis / genetics; Bacillus anthracis / metabolism; Bacterial Vaccines / genetics; Bacterial Vaccines / metabolism; Botulinum Toxins, Type A / genetics; Botulinum Toxins, Type A / metabolism; Botulism / microbiology; Botulism / prevention & control; Chromosomes, Bacterial / genetics; Chromosomes, Bacterial / metabolism; Clostridium botulinum / genetics; Clostridium botulinum / metabolism; Gene Expression; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / metabolism
TL;DR:
It is demonstrated that chromosomal integrative recombinant strains are promising vaccine delivery vehicles when targeted into high-expression chromosomal regions and eliminate the requirement for antibiotic selective maintenance of recombinant plasmids.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2016 review
Applications of CRISPR technologies in research and beyond
[Review of ]. NATURE BIOTECHNOLOGY, 34(9), 933–941.
TL;DR:
Programmable DNA cleavage using CRISPR–Cas9 enables efficient, site-specific genome engineering in single cells and whole organisms and is being used to expedite crop and livestock breeding, engineer new antimicrobials and control disease- carrying insects with gene drives.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2016 journal article
The effect of spray-drying parameters on the flavor of nonfat dry milk and milk protein concentrate 70%
JOURNAL OF DAIRY SCIENCE, 99(12), 9598–9610.
author keywords: nonfat dry milk; milk protein concentrate; process parameter; flavor
MeSH headings : Animals; Flavoring Agents; Food Handling; Milk / chemistry; Milk Proteins; Taste
TL;DR:
Results demonstrate that increasing inlet temperatures and solids concentration during spray drying decrease off-flavor intensities in NFDM and MPC70 even though the heat treatment is greater compared with low temperature and low solids.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2016 journal article
Short communication: The effect of raw milk cooling on sensory perception and shelf life of high-temperature, short-time (HTST)-pasteurized skim milk
JOURNAL OF DAIRY SCIENCE, 99(12), 9659–9667.
author keywords: milk; raw milk; cooling; pasteurization; shelf life; consumer
MeSH headings : Animals; Food Handling; Milk; Pasteurization; Temperature; Time Factors
TL;DR:
The results suggest that pasteurized milk quality is due to a combination of many factors, and raw milk cooling rate is not the most important factor affecting milk quality when raw milkquality is excellent.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2016 journal article
Genomic evolution of lactic acid bacteria: From single gene function to the pan-genome
Biotechnology of Lactic Acid Bacteria: Novel Applications, 2nd Edition, 32–54.
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
Source: NC State University Libraries
Added: August 6, 2018
2016 journal article
Evaluation of whey, milk, and delactosed permeates as salt substitutes
JOURNAL OF DAIRY SCIENCE, 99(11), 8687–8698.
author keywords: permeate; salt substitute; acid whey
MeSH headings : Adult; Animals; Cheese / analysis; Consumer Behavior; Female; Food Analysis; Food Handling; Food Quality; Gas Chromatography-Mass Spectrometry; Humans; Lactose / analysis; Male; Middle Aged; Milk / chemistry; Milk Proteins / analysis; Sodium Chloride, Dietary / analysis; Solid Phase Microextraction; Taste; Volatile Organic Compounds / analysis; Whey / chemistry; Young Adult
TL;DR:
The results demonstrate the potential for milk, whey, and delactosed permeates from different whey streams to be used as salt substitutes in product applications.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2016 journal article
Condensed milk storage and evaporation affect the flavor of nonfat dry milk
JOURNAL OF DAIRY SCIENCE, 99(12), 9586–9597.
author keywords: nonfat; dry milk; unit operations; flavor
MeSH headings : Animals; Desiccation; Flavoring Agents; Food Handling; Milk; Taste
TL;DR:
Results demonstrate that off-flavors are significantly reduced when RO is used in place of E and storage of condensed milk is avoided.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2016 journal article
Comparison of jaw tracking by single video camera with 3D electromagnetic system
JOURNAL OF FOOD ENGINEERING, 190, 22–33.
author keywords: Chewing; Mastication; Video tracking; Food; Sensory; Consumer
UN Sustainable Development Goal Categories
5. Gender Equality
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2016 journal article
An ISO-Protein Model Food System for Evaluating Food Texture Effects
JOURNAL OF TEXTURE STUDIES, 47(5), 377–391.
author keywords: Whey protein; oral processing; model foods; sensory; texture
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2016 journal article
AcmB Is an S-Layer-Associated beta-N-Acetylglucosaminidase and Functional Autolysin in Lactobacillus acidophilus NCFM
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 82(18), 5687–5697.
MeSH headings : Acetylglucosaminidase / genetics; Acetylglucosaminidase / metabolism; Bacterial Adhesion; Bacteriolysis; Cell Division; Cell Wall / chemistry; Computational Biology; Gene Deletion; Genetic Complementation Test; Lactobacillus acidophilus / enzymology; Lactobacillus acidophilus / genetics; Membrane Glycoproteins / genetics; Membrane Glycoproteins / metabolism; N-Acetylmuramoyl-L-alanine Amidase / genetics; N-Acetylmuramoyl-L-alanine Amidase / metabolism; Peptidoglycan / analysis
TL;DR:
It is demonstrated that multiple autolysins, responsible for breaking down the cell wall during cell division, are associated with the S-layer, the most widely used probiotic microbes incorporated in many dairy foods and dietary supplements.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2016 review
Toward Determining Best Practices for Recruiting Future Leaders in Food Science and Technology
[Review of ]. JOURNAL OF FOOD SCIENCE EDUCATION, 15(1), 9–13.
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
4. Quality Education
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2016 review
Stuck in the Middle: Fibronectin-Binding Proteins in Gram-Positive Bacteria
[Review of ]. FRONTIERS IN MICROBIOLOGY, 7.
author keywords: fibronectin; Lactobacillus; Acidophifus; lactobacilli; streptococci
TL;DR:
The interaction between human fibronectin and bacterial adhesins is described by highlighting the FnBPs of Gram-positive pathogens and commensals by providing an overview of the occurrence and diversity of FnBBP with a focus on the model pathogenic organisms in which FnBPS are most characterized.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2016 journal article
Short communication: The effect of liquid storage on the flavor of whey protein concentrate
JOURNAL OF DAIRY SCIENCE, 99(6), 4303–4308.
author keywords: whey protein; flavor; bleaching; storage
MeSH headings : Animals; Color; Flavoring Agents; Hydrogen Peroxide; Milk Proteins; Taste; Whey Proteins
TL;DR:
Results confirm that liquid storage increases off-flavors in spray-dried protein but to a variable degree, depending on whether bleaching has been applied, and if liquid storage is necessary, bleached WPC80 should be stored as liquid whey and unbleaching WPC 80 should be storage as liquid WPC to mitigate off- Flavors.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2016 journal article
Flavor and stability of milk proteins
JOURNAL OF DAIRY SCIENCE, 99(6), 4325–4346.
author keywords: milk protein; flavor; shelf life
MeSH headings : Animals; Flavoring Agents; Food Handling; Gas Chromatography-Mass Spectrometry; Milk Proteins; Taste; Whey Proteins
TL;DR:
Flavored milk proteins developed animal and burnt sugar flavors over time and solubility of MPC decreased and furosine concentration increased with storage time and temperature, with storage temperature having the greatest effect.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
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