Tobias Kaeser

Also known as: Tobias Käser; Toby Kaeser

https://orcid.org/0000-0001-8764-7971

Swine Immunology, Chlamydia, Vaccine development

I studied biology at the Eberhard Karls University Tübingen (Germany) with a focus on molecular biology, virology and microbiology. Having become interested in immunology, I decided to perform my Diploma thesis and subsequently my PhD thesis at an immunological institute and thus joined the Institute of Immunology at the Vetmeduni Vienna. The focus of my theses was the characterisation of porcine regulatory T cells (Tregs). My following postdoctoral work was delving deeper into the function of Tregs, focusing on their part in Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) infection. Besides, I was trying to identify new markers for several T-helper cell subsets in swine and to improve flow cytometry for veterinary medicine. Thereby, we became able to characterize porcine T-helper cell subsets and Tregs phenotypically and functionally by up to eight-color flow cytometry. In addition, I was an active member of the Austrian Society of Cytometry and participated in the planning and in the execution of basic and advanced courses and seminars. In September 2013, I moved to Saskatoon, Canada to work at VIDO to extend my knowledge on host-pathogen interactions. We were using the pig as a large animal model to study the cellular immune response on intracellular chlamydia infection. Joining this well-recognized institute gave me the ability to broaden my skills including the isolation and culture of bacteria and host cells, immunohistochemistry, and confocal microscopy. The insight into a new institution with various up-to-date methods and a wide experience with vaccine development made this decision a great success. In June 2016, I started as an Assistant Professor in swine immunology at the College of Veterinary Medicine at the North Carolina State University in Raleigh, North Carolina. My lab focuses on swine health and using the pig as an animal model for biomedical research. We currently study the development of immunological memory to the major pig virus PRRSV (Porcine Reproductive and Respiratory Syndrome Virus) and use the pig as a model to study chlamydia infections and food allergy. Other Experience and Professional Memberships International Society Memberships - Member, American Association of Veterinary Immunologists (AAVI) - Member, Edward Jenner Vaccine Society - Member, Chlamydia Basic Research Society - Member, German Society of Immunology (DGfI) - Member, Austrian Society for Cytometry (ÖGfZ) Editorial Roles and Board Positions - AAVI Board member - Guest editor for “Vaccine” - Veterinary Science and Research editorial board member Ad hoc review work for 17 international journals and funding agencies including - USDA/APHIS - National Pork Board - NSERC - PLOS One - Vaccine - Frontiers Immunology - Veterinary Research - Veterinary Immunol. Immunopathol. - Cytometry Part A - Helyion - Microbes and Infection Honors 2015: Successful application for the three year “Vaccine and Edward Jenner Vaccine Society Young Investigator Program (YIP)” (http://www.journals.elsevier.com/vaccine/tobias-kaser/) 2010: Successful application for participation at the „60th Lindau Nobel Laureate Meeting“, Lindau (GER) 2010: Successful application for the Post-Doc program sponsored by the Vetmeduni Vienna on the project “Role of Tregs in PRRSV infected swine” 2009: “Most-cited junior scientist 2008” award of the Vetmeduni Vienna 2007/2009: Poster awards at the New Year’s reception of the Vetmeduni Vienna

Works (29)

2020 journal article

Mucosal Vaccination with UV-Inactivated Chlamydia suis in Pre-Exposed Outbred Pigs Decreases Pathogen Load and Induces CD4 T-Cell Maturation into IFN-γ+ Effector Memory Cells

Vaccines.

By: A. Amaral, K. Rahman, A. Kick, L. Cortes, J. Robertson, B. Kaltenboeck, V. Gerdts, C. O'Connell ...

Source: ORCID
Added: July 7, 2020

2019 journal article

Expression of T-Bet, Eomesodermin, and GATA-3 Correlates With Distinct Phenotypes and Functional Properties in Porcine gamma delta T Cells

FRONTIERS IN IMMUNOLOGY, 10.

By: I. Rodriguez-Gomez, S. Talker, T. Kaeser, M. Stadler, L. Reiter, A. Ladinig, J. Milburn, S. Hammer ...

Sources: Web Of Science, ORCID
Added: March 25, 2019

2019 journal article

The T-Cell Response to Type 2 Porcine Reproductive and Respiratory Syndrome Virus (PRRSV)

Viruses, 11(9), 796.

By: A. Kick, A. Amaral, L. Cortes, J. Fogle, E. Crisci, G. Almond, T. Käser

Source: ORCID
Added: August 30, 2019

2018 journal article

Contribution of the swine model in the study of human sexually transmitted infections

Infection, Genetics and Evolution, 66, 346–360.

By: T. Käser, F. Renois, H. Wilson, T. Cnudde, V. Gerdts, J. Dillon, G. Jungersen, J. Agerholm, F. Meurens

Source: ORCID
Added: February 11, 2019

2018 journal article

Expression of pattern recognition receptors in porcine uterine epithelial cells in vivo and in culture

Veterinary Immunology and Immunopathology, 202, 1–10.

By: G. Hamonic, J. Pasternak, N. Forsberg, T. Käser & H. Wilson

Sources: Crossref, ORCID
Added: February 18, 2020

2018 journal article

First Insights Into Within Host Translocation of the Bacillus cereus Toxin Cereulide Using a Porcine Model

FRONTIERS IN MICROBIOLOGY, 9.

By: T. Bauer, W. Sipos, T. Stark, T. Kaeser, C. Knecht, R. Brunthaler, A. Saalmueller, T. Hofmann, M. Ehling-Schulz

Sources: NC State University Libraries, ORCID
Added: November 19, 2018

2017 journal article

Challenges and efforts in vaccine development and distribution

Vaccine.

By: T. Käser

Source: ORCID
Added: February 11, 2019

2017 journal article

Chlamydia suis and Chlamydia trachomatis induce multifunctional CD4 T cells in pigs

Vaccine, 35(1), 91–100.

By: T. Käser, J. Pasternak, M. Delgado-Ortega, G. Hamonic, K. Lai, J. Erickson, S. Walker, J. Dillon, V. Gerdts, F. Meurens

Source: ORCID
Added: February 11, 2019

2016 journal article

Expression of T-bet, Eomesodermin and GATA-3 in porcine αβ T cells

Developmental and Comparative Immunology, 60, 115–126.

By: I. Rodríguez-Gómez, S. Talker, T. Käser, M. Stadler, S. Hammer, A. Saalmüller, W. Gerner

Source: ORCID
Added: February 11, 2019

2016 journal article

Extended semen for artificial insemination in swine as a potential transmission mechanism for infectious Chlamydia suis

Theriogenology, 86(4), 949–956.

By: G. Hamonic, J. Pasternak, T. Käser, F. Meurens & H. Wilson

Sources: ORCID, Crossref
Added: February 11, 2019

2016 journal article

Flow cytometry as an improved method for the titration of Chlamydiaceae and other intracellular bacteria

Cytometry Part A, 89(5), 451–460.

By: T. Käser, J. Pasternak, G. Hamonic, M. Rieder, K. Lai, M. Delgado-Ortega, V. Gerdts, F. Meurens

Source: ORCID
Added: February 11, 2019

2016 journal article

Ubiquitous LEA29Y Expression Blocks T Cell Co-Stimulation but Permits Sexual Reproduction in Genetically Modified Pigs

PLOS ONE, 11(5), e0155676.

By: A. Bähr, T. Käser, E. Kemter, W. Gerner, M. Kurome, W. Baars, N. Herbach, K. Witter ...

Ed(s): N. Câmara

Sources: ORCID, Crossref
Added: February 11, 2019

2015 journal article

Natural and inducible Tregs in swine: Helios expression and functional properties

Developmental and Comparative Immunology, 49(2), 323–331.

By: T. Käser, K. Mair, S. Hammer, W. Gerner & A. Saalmüller

Source: ORCID
Added: February 11, 2019

2015 journal article

PRRSV-infected monocyte-derived dendritic cells express high levels of SLA-DR and CD80/86 but do not stimulate PRRSV-naïve regulatory T cells to proliferate

Veterinary Research, 46(1).

By: I. Rodríguez-Gómez, T. Käser, J. Gómez-Laguna, B. Lamp, L. Sinn, T. Rümenapf, L. Carrasco, A. Saalmüller, W. Gerner

Sources: ORCID, Crossref
Added: February 11, 2019

2015 journal article

Porcine retinal cell line VIDO R1 and Chlamydia suis to modelize ocular chlamydiosis

Veterinary Immunology and Immunopathology, 166(3-4), 95–107.

By: T. Käser, T. Cnudde, G. Hamonic, M. Rieder, J. Pasternak, K. Lai, S. Tikoo, H. Wilson, F. Meurens

Source: ORCID
Added: February 11, 2019

2015 journal article

The systemic multiplication of Gallibacterium anatis in experimentally infected chickens is promoted by immunosuppressive drugs which have a less specific effect on the depletion of leukocytes

Veterinary Immunology and Immunopathology, 166(1-2), 22–32.

By: S. Paudel, C. Hess, P. Wernsdorf, T. Käser, S. Meitz, E. Jensen-Jarolim, M. Hess, D. Liebhart

Source: ORCID
Added: February 11, 2019

2014 journal article

The porcine innate immune system: An update

Developmental and Comparative Immunology, 45(2), 321–343.

By: K. Mair, C. Sedlak, T. Käser, A. Pasternak, B. Levast, W. Gerner, A. Saalmüller, A. Summerfield ...

Source: ORCID
Added: February 11, 2019

2013 journal article

Human Placental Alkaline Phosphatase as a Tracking Marker for Bone Marrow Mesenchymal Stem Cells

BioResearch Open Access, 2(5), 346–355.

By: E. Balmayor, M. Flicker, T. Käser, A. Saalmüller & R. Erben

Sources: Crossref, ORCID
Added: July 20, 2019

2013 journal article

Immunohistochemical characterization of type II pneumocyte proliferation after challenge with type I porcine reproductive and respiratory syndrome virus

Journal of Comparative Pathology, 149(2-3), 322–330.

By: G. Balka, A. Ladinig, M. Ritzmann, A. Saalmüller, W. Gerner, T. Käser, C. Jakab, M. Rusvai, H. Weißenböck

Source: ORCID
Added: February 11, 2019

2013 journal article

Phenotypic maturation of porcine NK- and T-cell subsets

Developmental and Comparative Immunology, 40(1), 51–68.

By: S. Talker, T. Käser, K. Reutner, C. Sedlak, K. Mair, H. Koinig, R. Graage, M. Viehmann ...

Source: ORCID
Added: February 11, 2019

2012 journal article

Current knowledge on porcine regulatory T cells

Veterinary Immunology and Immunopathology, 148(1-2), 136–138.

By: T. Käser, W. Gerner, K. Mair, S. Hammer, M. Patzl & A. Saalmüller

Source: ORCID
Added: February 11, 2019

2012 journal article

Porcine T-helper and regulatory T cells exhibit versatile mRNA expression capabilities for cytokines and co-stimulatory molecules

Cytokine, 60(2), 400–409.

By: T. Käser, A. Müllebner, R. Hartl, S. Essler, A. Saalmüller & J. Catharina Duvigneau

Source: ORCID
Added: February 11, 2019

2011 journal article

Porcine regulatory T cells: Mechanisms and T-cell targets of suppression

Developmental & Comparative Immunology, 35(11), 1166–1172.

By: T. Käser, W. Gerner & A. Saalmüller

Sources: ORCID, Crossref
Added: February 11, 2019

2009 journal article

Molecular characterisation of porcine Forkhead-box p3 (Foxp3)

Veterinary Immunology and Immunopathology, 132(2-4), 275–281.

By: K. Bolzer, T. Käser, A. Saalmüller & S. Hammer

Source: ORCID
Added: February 11, 2019

2009 journal article

Porcine T lymphocytes and NK cells - An update

Developmental and Comparative Immunology, 33(3), 310–320.

By: W. Gerner, T. Käser & A. Saalmüller

Source: ORCID
Added: February 11, 2019

2008 journal article

Detection of Foxp3 protein expression in porcine T lymphocytes

Veterinary Immunology and Immunopathology, 125(1-2), 92–101.

By: T. Käser, W. Gerner, S. Hammer, M. Patzl & A. Saalmüller

Source: ORCID
Added: February 11, 2019

2008 journal article

Detection of intracellular antigens in porcine PBMC by flow cytometry: A comparison of fixation and permeabilisation reagents

Veterinary Immunology and Immunopathology, 121(3-4), 251–259.

Source: ORCID
Added: February 11, 2019

2008 journal article

Phenotypic and functional characterisation of porcine CD4+CD25high regulatory T cells

Veterinary Immunology and Immunopathology, 122(1-2), 153–158.

By: T. Käser, W. Gerner, S. Hammer, M. Patzl & A. Saalmüller

Source: ORCID
Added: February 11, 2019

journal article

Grouping Pig-Specific Responses to Mitogen with Similar Responder Animals may Facilitate the Interpretation of Results Obtained in an Out-Bred Animal Model

Pasternak, J. A., Hon Ng, S. L., Käser, T., Meurens, F., & Wilson, H. L. Journal of Vaccines and Vaccination, 5, 1–9.

By: J. Pasternak, S. Hon Ng, T. Käser, F. Meurens & H. Wilson

Source: NC State University Libraries
Added: July 11, 2019

Employment

2016 - present

North Carolina State University Raleigh, NC, US
Assistant Professor in Swine Immunology College of Veterinary Medicine - Population Health and Pathobiology

2013 - 2016

University of Saskatchewan Saskatoon, SK, CA
PostDoc (Establishment of the pig as a model to study chlamydia infections) Vaccine and Infectious Disease Organization (VIDO-InterVac)

2010 - 2013

University of Veterinary Medicine Vienna Vienna, AT
PostDoc (Role of Tregs in Porcine Reproductive and Respiratory Syndrome Virus infections) Institute of Immunology

Education

2006 - 2010

University of Veterinary Medicine Vienna Vienna, AT
PhD (Immunology) Institute of Immunology

1999 - 2004

Eberhard Karls Universität Tübingen Tubingen, Baden-Württemberg, DE
Diploma in Biology (Molecular Biology, Virology, Microbiology)