Tobias Ernst Kaeser

Also known as: Tobias Käser; Toby Kaeser

Immunology, Chlamydia, Vaccine development, Food allergy, Animal model

I studied biology at the Eberhard Karls University Tübingen (Germany) with a focus on molecular biology, virology and microbiology. Having become interested in immunology, I decided to perform my Diploma thesis and subsequently my PhD thesis at an immunological institute and thus joined the Institute of Immunology at the Vetmeduni Vienna. The focus of my theses was the characterisation of porcine regulatory T cells (Tregs). My following postdoctoral work was delving deeper into the function of Tregs, focusing on their part in Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) infection. Besides, I was trying to identify new markers for several T-helper cell subsets in swine and to improve flow cytometry for veterinary medicine. Thereby, we became able to characterize porcine T-helper cell subsets and Tregs phenotypically and functionally by up to eight-color flow cytometry. In addition, I was an active member of the Austrian Society of Cytometry and participated in the planning and in the execution of basic and advanced courses and seminars. In September 2013, I moved to Saskatoon, Canada to work at VIDO to extend my knowledge on host-pathogen interactions. We were using the pig as a large animal model to study the cellular immune response on intracellular chlamydia infection. Joining this well-recognized institute gave me the ability to broaden my skills including the isolation and culture of bacteria and host cells, immunohistochemistry, and confocal microscopy. The insight into a new institution with various up-to-date methods and a wide experience with vaccine development made this decision a great success. In June 2016, I started as an Assistant Professor in swine immunology at the College of Veterinary Medicine at the North Carolina State University in Raleigh, North Carolina. My lab focuses on swine health and using the pig as an animal model for biomedical research. We currently study the development of immunological memory to the major pig virus PRRSV (Porcine Reproductive and Respiratory Syndrome Virus) and use the pig as a model to study chlamydia infections and food allergy. In 2023, I started my new position as Assistant Professor in Immunology at the University of Veterinary Medicine (vetmeduni) Vienna. Here I plan to continue my work towards the development of a vaccine against Chlamydia trachomatis as well as my research on food allergy and PRRSV. Other Experience and Professional Memberships International Society Memberships - Member, American Association of Veterinary Immunologists (AAVI) - Member, Edward Jenner Vaccine Society - Member, Chlamydia Basic Research Society - Member, German Society of Immunology (DGfI) - Member, Austrian Society for Cytometry (ÖGfZ) Editorial Roles and Board Positions - Frontiers Microbiology: Associate Editor - Frontiers Immunology: Editorial Board member – section Comparative Immunology - Molecular Immunology: Editorial Board member Ad hoc review work for 17 international journals and funding agencies including Funding agencies: - National Institutes of Health (NIH, USA) - US Department of Agriculture (USDA, USA) - National Pork Board (USA) - NSERC (Natural Sciences and Engineering Research Council, Canada) - BBSRC (Biotechnology and Biological Sciences Research Council, UK) - WWTF (Vienna Science and Technology Fund, Austria) Journals (selected): - Frontiers in Immunology - Frontiers in Microbiology - PLOS One - Vaccine - Veterinary Research - Veterinary Immunol. Immunopathol. - Cytometry Part A - Helyion - Microbes and Infection Honors 2021/22:Nomination for Faculty Scholar of NC State University 2021: Recipient of the “Goodnight Early Career Innovators” Award 2021: Nomination for the Zoetis Distinguished Veterinary Teacher Award 2019: Invitation to hold the keynote lecture “2019 Michael P. Murtaugh Memorial Lecture in Immunology” lecture at the NA PRRS Symposium (Chicago, IL): “The adaptive immune response to PRRSV” 2015-2018: Participation in the “Vaccine and Edward Jenner Vaccine Society Young Investigator Program (YIP)” 2010: Successful application for participation at the “60th Lindau Nobel Laureate Meeting”, Lindau (GER) 2010: Successful application for the Post-Doc program sponsored by the Vetmeduni Vienna on the project “Role of Tregs in PRRSV infected swine” 2009: “Most-cited junior scientist 2008” award of the Vetmeduni Vienna

Works (41)

Updated: May 22nd, 2024 05:52

2024 journal article

A TriAdj-Adjuvanted <i>Chlamydia trachomatis</i> CPAF Protein Vaccine Is Highly Immunogenic in Pigs

VACCINES, 12(4).

By: J. Proctor n, M. Stadler*, L. Cortes n, D. Brodsky n, L. Poisson n, V. Gerdts*, A. Smirnov n, T. Smirnova n ...

author keywords: Chlamydia trachomatis; vaccination; adjuvant; chlamydial-protease-like activity factor; animal model; swine; one health
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: April 22, 2024

2023 journal article

Testable Candidate Immune Correlates of Protection for Porcine Reproductive and Respiratory Syndrome Virus Vaccination

Vaccines, 11(3), 594.

By: A. Kick n, A. Grete*, E. Crisci n, G. Almond n & T. Käser n

author keywords: vaccination; PRRSV; correlates of protection; humoral immunity; IgG; neutralizing antibodies; T cell; IFN-gamma
TL;DR: Applying the breadth of work on human diseases and CoP to PRRSV research, four hypotheses are advocated for peer review and evaluation as appropriate testable CoP and can direct future vaccine design and improve vaccine candidate evaluation. (via Semantic Scholar)
Sources: ORCID, Web Of Science, NC State University Libraries, Crossref
Added: March 7, 2023

2022 journal article

Allogeneic and xenogeneic lymphoid reconstitution in a RAG2(-/-)IL2RG(y/-) severe combined immunodeficient pig: A preclinical model for intrauterine hematopoietic transplantation

FRONTIERS IN VETERINARY SCIENCE, 9.

By: R. Sper n, J. Proctor n, O. Lascina n, L. Guo n, K. Polkoff n, T. Kaeser n, S. Simpson n, L. Borst n ...

author keywords: severe combined immunodeficiency; pig; xenotransplantation; allotransplantation; hematopoietic; RAG2; IL2RG; transgenic
TL;DR: The data shows that the pig thymus provides a microenvironment conducive to engraftment, survival and development of human T-cells and provides evidence that the developing T-cell compartment can be populated to a significant extent by human cells in large animals. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: November 14, 2022

2022 journal article

Heterologous vaccine immunogenicity, efficacy, and immune correlates of protection of a modified-live virus porcine reproductive and respiratory syndrome virus vaccine

Frontiers in Microbiology, 13.

By: J. Proctor n, I. Wolf n, D. Brodsky n, L. Cortes n, A. Frias-De-Diego n, G. Almond n, E. Crisci n, T. Negrão Watanabe n, J. Hammer*, T. Käser n

Contributors: E. Crisci n

author keywords: porcine reproductive and respiratory syndrome virus (PRRSV); correlates of protection (CoP); vaccination; swine; immunity; pig
TL;DR: Overall, the Prevacent elicited various degrees of efficacy and immunogenicity against four heterologous and phylogenetically distant strains of PRRSV-2, and correlation analyses revealed that the (non-PRRSV strain-specific) serum IgG levels and the PRRSv strain- specific CD4 T-cell response were the best immune correlates of protection. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: September 25, 2022

2022 journal article

Immunologic and pathologic characterization of a novel swine biomedical research model for eosinophilic esophagitis

FRONTIERS IN ALLERGY, 3.

By: L. Cortes n, D. Brodsky n, C. Chen, T. Pridgen n, J. Odle n, D. Snider n, G. Cruse n, A. Putikova* ...

author keywords: pig; food allergy; animal model; immunology; gastrointestinal; eosinophilic esophagitis
TL;DR: Swine is established as a relevant large animal model for translational biomedical research in EoE with the potential to facilitate development of therapeutics and it is demonstrated that the sensitization and oral challenge protocol not only induces the underlying immune markers but also the micro- and macro-pathological hallmarks of human EiE. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: September 25, 2023

2021 journal article

Host-Pathogen Interactions of Chlamydia trachomatis in Porcine Oviduct Epithelial Cells

PATHOGENS, 10(10).

author keywords: Chlamydia trachomatis; porcine oviduct epithelial cell; developmental cycle; innate immune response; claudin-4; tight junctions
TL;DR: It is shown that primary pOECs represent an excellent in vitro model for research into Ct pathogenesis, cell biology and immunity and induces an immune response to Ct similar to human cells. (via Semantic Scholar)
Sources: ORCID, Web Of Science
Added: October 6, 2021

2021 journal article

Maternal Autogenous Inactivated Virus Vaccination Boosts Immunity to PRRSV in Piglets

VACCINES, 9(2).

author keywords: maternal vaccination; autogenous inactivated vaccine; transfer of immunity; humoral immune response; cell-mediated immune response; T cells; PRRSV; swine; IFN-gamma producing B cells; CD4 T-EMRA cells
TL;DR: It is demonstrated that maternal AIV vaccination can improve protection of pre-weaning piglets against PRRSV-2 and shows the importance of transferring neutralizing antibodies to piglets, and it introduces two novel immune cell subsets in pigs—IFN-γ producing CD21α+ B cells and CD8α−CCR7− CD4 T cells. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: February 1, 2021

2021 journal article

Swine as biomedical animal model for T-cell research-Success and potential for transmittable and non-transmittable human diseases

MOLECULAR IMMUNOLOGY, 135, 95–115.

author keywords: Pig Animal model; T cells; Vaccine development; Infectious disease; Virus; Bacteria; Parasites; Allergy; Cancer; Transplantation
MeSH headings : Animals; Biomedical Research; Communicable Diseases / immunology; Disease Models, Animal; Haplorhini; Mice; Swine; Swine Diseases / immunology; T-Lymphocytes / immunology
TL;DR: This review summarizes the swine model for biomedical research with focus on T cells and contrasts it to the more commonly used mouse and non-human primate model before describing the current capabilities to characterize and extend knowledge on porcine T cells. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: ORCID, Web Of Science
Added: April 18, 2021

2020 article

Maternal Autogenous Inactivated Virus Vaccination Boosts the Piglet Humoral and Cell-Mediated Immunity to PRRSV via Transfer of Neutralizing Antibodies and Interferon-Gamma Producing B Cells

Kick, A. R., Wolfe, Z. C., Amaral, A. F., Cortes, L. M., Almond, G. W., Crisci, E., … Käser, T. (2020, December 3). (Vol. 12). Vol. 12.

By: A. Kick n, Z. Wolfe n, A. Amaral n, L. Cortes n, G. Almond n, E. Crisci n, P. Gauger*, J. Pittman, T. Käser n

TL;DR: It is demonstrated that maternal AIV vaccination can improve protection of pre-weaning piglets against PRRSV-2 and shows the importance of transferring neutralizing antibodies to piglets; and it introduces two novel immune cell subsets in pigs. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Source: ORCID
Added: December 3, 2020

2020 journal article

Mucosal Vaccination with UV-Inactivated <i>Chlamydia suis</i> in Pre-Exposed Outbred Pigs Decreases Pathogen Load and Induces CD4 T-Cell Maturation into IFN-γ<sup>+</sup> Effector Memory Cells

Vaccines, 8(3), 353.

By: A. Amaral n, K. Rahman*, A. Kick n, L. Cortes n, J. Robertson n, B. Kaltenboeck*, V. Gerdts*, C. O'Connell* ...

author keywords: chlamydia trachomatis; chlamydia suis; large animal model; swine; translational research; vaccine development; TriAdj; vaccination; immunology; T cells; effector memory
TL;DR: Results indicate that Cs vaccination of pre-exposed pigs effectively boosts a non-protective immune response induced by natural infection; moreover, they suggest that a similar approach could be applied to human vaccine trials. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: ORCID
Added: July 7, 2020

2019 journal article

Expression of T-Bet, Eomesodermin, and GATA-3 Correlates With Distinct Phenotypes and Functional Properties in Porcine gamma delta T Cells

FRONTIERS IN IMMUNOLOGY, 10.

By: I. Rodriguez-Gomez*, S. Talker*, T. Kaeser, M. Stadler*, L. Reiter*, A. Ladinig*, J. Milburn*, S. Hammer* ...

author keywords: gamma delta T cells; swine; pig; T-bet; Eomes; GATA-3
MeSH headings : Animals; GATA3 Transcription Factor / biosynthesis; GATA3 Transcription Factor / immunology; Phenotype; Receptors, Antigen, T-Cell, gamma-delta / immunology; Swine / immunology; T-Box Domain Proteins / biosynthesis; T-Box Domain Proteins / immunology; T-Lymphocyte Subsets / immunology; T-Lymphocyte Subsets / metabolism
TL;DR: A role for GATA-3 is suggested in the development of γδ-thymocytes and in the function of peripheral CD2−CD8α−/dimCD27+perforin− γ´ T cells and the unique phenotype of these porcine T cells suggests that they represent a thymus-derived separate lineage in the pig. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: March 25, 2019

2019 journal article

The T-Cell Response to Type 2 Porcine Reproductive and Respiratory Syndrome Virus (PRRSV)

Viruses, 11(9), 796.

By: A. Kick n, A. Amaral n, L. Cortes n, J. Fogle n, E. Crisci n, G. Almond n, T. Käser n

author keywords: PRRSV; T cells; adaptive immunity; treg; thelper; Cytotoxic proliferation; TCR-gamma delta; IFN-gamma
MeSH headings : Adaptive Immunity; Animals; Kinetics; Lung / immunology; Lung / virology; Lymph Nodes / immunology; Lymph Nodes / virology; Porcine Reproductive and Respiratory Syndrome / immunology; Porcine Reproductive and Respiratory Syndrome / pathology; Porcine Reproductive and Respiratory Syndrome / virology; Porcine respiratory and reproductive syndrome virus / growth & development; Porcine respiratory and reproductive syndrome virus / immunology; Porcine respiratory and reproductive syndrome virus / pathogenicity; Receptors, Antigen, T-Cell, gamma-delta / metabolism; Receptors, Lymphocyte Homing / metabolism; Swine; T-Lymphocyte Subsets / immunology; T-Lymphocyte Subsets / virology; T-Lymphocytes / immunology; T-Lymphocytes / virology; Vaccines, Attenuated; Viral Vaccines / immunology; Viremia / immunology; Viremia / virology
TL;DR: The results demonstrate an important role for T cells in this homo- and heterologous response to type-2 PRRSV, and the T-helper cells were the main responders during viremia, while the cytotoxic T lymphocyte response was the strongest at the site of infection—the lung and bronchoalveolar lavage. (via Semantic Scholar)
Source: ORCID
Added: August 30, 2019

2018 journal article

Contribution of the swine model in the study of human sexually transmitted infections

Infection, Genetics and Evolution, 66, 346–360.

author keywords: Pig model; Chlamydia trachomatis; Trichomonas vaginalis; Neisseria gonorrhoeae; Hepatitis viruses; Herpesviruses
MeSH headings : Animals; Disease Models, Animal; Disease Susceptibility; Female; Hormones / metabolism; Humans; Male; Sex Factors; Sexually Transmitted Diseases / etiology; Sexually Transmitted Diseases / metabolism; Sexually Transmitted Diseases / prevention & control; Swine
TL;DR: With all its advantages and the highly improved toolbox, the porcine model can play a crucial role in STI research and open the door to new exciting discoveries. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: ORCID
Added: February 11, 2019

2018 journal article

Expression of pattern recognition receptors in porcine uterine epithelial cells in vivo and in culture

Veterinary Immunology and Immunopathology, 202, 1–10.

By: G. Hamonic*, J. Pasternak*, N. Forsberg*, T. Käser n & H. Wilson*

author keywords: Epithelial; Toll-like receptor; Pattern recognition receptor; Innate immunity; Pig; Uterus
MeSH headings : Acetylmuramyl-Alanyl-Isoglutamine / pharmacology; Animals; Cells, Cultured; Cytokines / metabolism; Epithelial Cells / immunology; Epithelial Cells / metabolism; Female; Immunity, Innate; Lipopolysaccharides / pharmacology; Oligodeoxyribonucleotides / pharmacology; Poly I-C / pharmacology; Real-Time Polymerase Chain Reaction; Receptors, Pattern Recognition / metabolism; Swine; Toll-Like Receptor 3 / metabolism; Toll-Like Receptor 9 / metabolism; Toll-Like Receptors / metabolism; Uterus / cytology
TL;DR: Results indicate that pig uterine epithelial cells are functional innate immune cells that may act as sentinels to protect against uterine infection. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: February 18, 2020

2018 journal article

First Insights Into Within Host Translocation of the Bacillus cereus Toxin Cereulide Using a Porcine Model

FRONTIERS IN MICROBIOLOGY, 9.

By: T. Bauer*, W. Sipos*, T. Stark*, T. Kaeser, C. Knecht*, R. Brunthaler*, A. Saalmueller, T. Hofmann*, M. Ehling-Schulz*

TL;DR: It is demonstrated that cereulide is able to cross the blood–brain–barrier, which may partially explain the cerebral effects reported from human intoxication cases, and typical diagnostic specimens used in human medicine, such as blood samples and urine, are not suitable for diagnostics of food borne Cereulide intoxications. (via Semantic Scholar)
Sources: NC State University Libraries, NC State University Libraries
Added: November 19, 2018

2017 journal article

Challenges and efforts in vaccine development and distribution

Vaccine.

Contributors: T. Käser n

MeSH headings : Biomedical Research; Health Resources; Humans; Influenza Vaccines; Influenza, Human; Pandemics
Source: ORCID
Added: February 11, 2019

2017 journal article

Chlamydia suis and Chlamydia trachomatis induce multifunctional CD4 T cells in pigs

Vaccine, 35(1), 91–100.

Contributors: T. Käser n, J. Pasternak*, M. Delgado-Ortega*, G. Hamonic*, K. Lai*, J. Erickson*, S. Walker*, J. Dillon*, V. Gerdts*, F. Meurens*

author keywords: Chlamydia suis; Chlamydia trachomatis; Animal model; Swine; Vaccine development; One Health
MeSH headings : Administration, Intravaginal; Animals; Antibodies, Bacterial / blood; Antibody Formation; CD4-Positive T-Lymphocytes / immunology; Chlamydia / immunology; Chlamydia / pathogenicity; Chlamydia Infections / immunology; Chlamydia Infections / microbiology; Chlamydia Infections / pathology; Chlamydia Infections / veterinary; Cytokines / metabolism; Host-Pathogen Interactions; Immunity, Cellular; Immunity, Humoral; Swine; Time Factors
TL;DR: The T-cell-mediated immune responses were triggered by both homologous or heterologous re-stimulation indicating that cross-protection between the two chlamydia species is possible and could bridge the gap to the clinical phase for C. trachomatis vaccine research. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: ORCID
Added: February 11, 2019

2016 journal article

Expression of T-bet, Eomesodermin and GATA-3 in porcine αβ T cells

Developmental and Comparative Immunology, 60, 115–126.

author keywords: T-bet; GATA-3; Eomesodermin; Swine; alpha beta T cells
TL;DR: The data so far support the hitherto reported roles for GATA-3 in T-cell development and T-bet for Th1 effector-differentiation, but question the role of Eomesodermin for memory formation of porcine T-cells. (via Semantic Scholar)
Source: ORCID
Added: February 11, 2019

2016 journal article

Extended semen for artificial insemination in swine as a potential transmission mechanism for infectious Chlamydia suis

Theriogenology, 86(4), 949–956.

author keywords: Artificial insemination; Biosecurity; Chlamydia; Herd health; Semen; Semen extender
MeSH headings : Animals; Chlamydia / isolation & purification; Insemination, Artificial / veterinary; Semen / microbiology; Semen Analysis / veterinary; Semen Preservation; Staining and Labeling; Swine / physiology; Time Factors
TL;DR: It is suggested that C suis remains viable and infectious during chilled storage and is globally unaffected by antibiotics in extender. (via Semantic Scholar)
Sources: ORCID, Crossref, NC State University Libraries
Added: February 11, 2019

2016 journal article

Flow cytometry as an improved method for the titration of Chlamydiaceae and other intracellular bacteria

Cytometry Part A, 89(5), 451–460.

By: T. Käser*, J. Pasternak*, G. Hamonic*, M. Rieder*, K. Lai*, M. Delgado-Ortega*, V. Gerdts*, F. Meurens*

Contributors: T. Käser*, J. Pasternak*, G. Hamonic*, M. Rieder*, K. Lai*, M. Delgado-Ortega*, V. Gerdts*, F. Meurens*

author keywords: Chlamydiaceae; Chlamydia suis; intracellular bacteria; titration; flow cytometry; FCM
MeSH headings : Cell Line; Chlamydiaceae / isolation & purification; Epithelial Cells / microbiology; Flow Cytometry / methods; Humans; Microscopy; Reproducibility of Results; Sensitivity and Specificity
TL;DR: Flow cytometry offers a fast, cheap, precise, and reproducible alternative for the titration of intracellular bacteria like C. suis as a representative of other intrACEllular bacteria. (via Semantic Scholar)
Source: ORCID
Added: February 11, 2019

2016 journal article

Ubiquitous LEA29Y Expression Blocks T Cell Co-Stimulation but Permits Sexual Reproduction in Genetically Modified Pigs

PLOS ONE, 11(5), e0155676.

By: A. Bähr*, T. Käser*, E. Kemter*, W. Gerner*, M. Kurome*, W. Baars*, N. Herbach*, K. Witter* ...

Contributors: A. Bähr*, T. Käser*, E. Kemter*, W. Gerner*, M. Kurome*, W. Baars*, N. Herbach*, K. Witter* ...

Ed(s): N. Câmara

MeSH headings : Abatacept / metabolism; Animals; Animals, Genetically Modified; Antigen-Presenting Cells / metabolism; Cloning, Organism; Conserved Sequence; Crosses, Genetic; Female; Fertilization in Vitro; Humans; Lymph Nodes / pathology; Lymphocyte Activation / immunology; Male; Promoter Regions, Genetic / genetics; Protein Binding; Reproduction / genetics; Sus scrofa / genetics; Sus scrofa / immunology; T-Lymphocytes / immunology
TL;DR: The analysis of LEA29Y expression in serum and multiple organs of CAG-LEA transgenic pigs revealed that these animals produce a biologically active transgenic product at a considerable level and they present with an immune system affected by transgene expression, but can be maintained until sexual maturity and propagated by assisted reproduction techniques. (via Semantic Scholar)
Sources: ORCID, Crossref, NC State University Libraries
Added: February 11, 2019

2015 journal article

Natural and inducible Tregs in swine: Helios expression and functional properties

Developmental and Comparative Immunology, 49(2), 323–331.

author keywords: Regulatory T cells; nTregs; iTregs; Helios; CD4(+); Pig
MeSH headings : Amino Acid Sequence; Animals; Biomarkers; CD3 Complex / biosynthesis; Cell Differentiation / immunology; Cell Line; Cell Proliferation; Cells, Cultured; Coculture Techniques; Forkhead Transcription Factors / metabolism; HEK293 Cells; Humans; Ikaros Transcription Factor / immunology; Interferon-gamma / biosynthesis; Interleukin-10 / metabolism; Interleukin-2 / immunology; Leukocytes, Mononuclear / immunology; Lymphocyte Activation / immunology; Molecular Sequence Data; Swine; T-Lymphocytes, Regulatory / cytology; T-Lymphocytes, Regulatory / immunology; Transforming Growth Factor beta / immunology; Transforming Growth Factor beta / metabolism
TL;DR: The cross-reactivity of an anti-mouse/human Helios mAb with porcine Helios demonstrates the suitability of Helios as an nTreg-iTreg differentiation marker and finds that Helios(-) Tregs displayed a phenotype of naive CD4(+) T cells in vivo, indicating thatHelios might rather serve as a Treg activation/differentiation marker. (via Semantic Scholar)
Source: ORCID
Added: February 11, 2019

2015 journal article

PRRSV-infected monocyte-derived dendritic cells express high levels of SLA-DR and CD80/86 but do not stimulate PRRSV-naïve regulatory T cells to proliferate

Veterinary Research, 46(1).

Contributors: I. Rodríguez-Gómez*, T. Käser*, J. Gómez-Laguna, B. Lamp*, L. Sinn*, T. Rümenapf*, L. Carrasco*, A. Saalmüller*, W. Gerner*

MeSH headings : Animals; B7-1 Antigen / immunology; B7-1 Antigen / metabolism; B7-2 Antigen / immunology; B7-2 Antigen / metabolism; Dendritic Cells / immunology; Dendritic Cells / metabolism; Forkhead Transcription Factors / genetics; Forkhead Transcription Factors / metabolism; Histocompatibility Antigens Class I; Histocompatibility Antigens Class II / immunology; Histocompatibility Antigens Class II / metabolism; Monocytes / metabolism; Porcine Reproductive and Respiratory Syndrome / immunology; Porcine Reproductive and Respiratory Syndrome / virology; Porcine respiratory and reproductive syndrome virus / physiology; Swine; T-Lymphocytes, Regulatory / immunology; T-Lymphocytes, Regulatory / metabolism
TL;DR: It is suggested that a more detailed characterisation of PRRSV-infected moDCs will lead to more consistent results across different laboratories andPRRSV strains as indicated by the major differences in SLA-DR and CD80/86 expression between PR RSV- Infected and non-infecting mo DCs present in the same microculture. (via Semantic Scholar)
Sources: ORCID, Crossref, NC State University Libraries
Added: February 11, 2019

2015 journal article

Porcine retinal cell line VIDO R1 and Chlamydia suis to modelize ocular chlamydiosis

Veterinary Immunology and Immunopathology, 166(3-4), 95–107.

By: T. Käser*, T. Cnudde*, G. Hamonic*, M. Rieder*, J. Pasternak*, K. Lai*, S. Tikoo*, H. Wilson*, F. Meurens*

Contributors: T. Käser*, T. Cnudde*, G. Hamonic*, M. Rieder*, J. Pasternak*, K. Lai*, S. Tikoo*, H. Wilson*, F. Meurens*

author keywords: Pig; Retina; Innate immune response; Chlamydia suis
MeSH headings : Animals; Cell Line; Chlamydia / physiology; Chlamydia Infections / microbiology; Chlamydia Infections / veterinary; Chlamydia trachomatis / physiology; Cytokines / metabolism; Disease Models, Animal; Eye Infections, Bacterial / microbiology; Eye Infections, Bacterial / veterinary; Humans; Real-Time Polymerase Chain Reaction / veterinary; Retina / cytology; Retina / microbiology; Swine; Trachoma / microbiology
TL;DR: The first steps in the host-pathogen-interactions of ocular C. suis infections in pigs are illustrated and show their similarity to C. trachomatis infections in humans, justifying further testing of pigs as an animal model for human trachoma. (via Semantic Scholar)
Source: ORCID
Added: February 11, 2019

2015 journal article

The systemic multiplication of Gallibacterium anatis in experimentally infected chickens is promoted by immunosuppressive drugs which have a less specific effect on the depletion of leukocytes

Veterinary Immunology and Immunopathology, 166(1-2), 22–32.

author keywords: Gallibacterium anatis; Chickens; Leukocyte quantification; Non-selective Immunosuppression
MeSH headings : Animals; Bacterial Load; Chickens / immunology; Corticosterone / pharmacology; Immunosuppressive Agents / pharmacology; Leukocyte Count; Leukocytes / drug effects; Pasteurellaceae / physiology; Pasteurellaceae Infections / veterinary; Poultry Diseases / immunology; Poultry Diseases / microbiology; Spleen / microbiology
TL;DR: Bacterial re-isolation and counts of colony forming units (CFU) showed that G. anatis colonization pattern in various organs, and the numbers of bacteria in trachea were not affected by immunosuppression, however, the treatments acutely increased CFU counts derived from the spleen, which demonstrates that chemically induced immunOSuppression intensifies systemic multiplication of G. Anatis in chickens. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Source: ORCID
Added: February 11, 2019

2014 journal article

The porcine innate immune system: An update

Developmental and Comparative Immunology, 45(2), 321–343.

By: K. Mair*, C. Sedlak*, T. Käser*, A. Pasternak*, B. Levast*, W. Gerner*, A. Saalmüller*, A. Summerfield ...

Contributors: K. Mair*, C. Sedlak*, T. Käser*, A. Pasternak*, B. Levast*, W. Gerner*, A. Saalmüller*, A. Summerfield ...

author keywords: Pig; Innate response; Animal model; Innate immune cells
MeSH headings : Animals; Immunity, Innate; Inflammation / immunology; Receptors, Cytoplasmic and Nuclear / metabolism; Receptors, Pattern Recognition / immunology; Sus scrofa / immunology; Swine / immunology; Swine Diseases / immunology
TL;DR: The porcine innate immune system in pigs is compared to that of humans, and the importance of the pig as model for human disease is emphasized. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Source: ORCID
Added: February 11, 2019

2013 journal article

Effect of Two Strains of PRRSV on the Expression of Differentiation Antigens of Monocyte-derived Dendritic Cells

Journal of Comparative Pathology, 148(1), 51.

By: I. Rodríguez-Gómez*, A. Burgara-Estrella*, E. Mateu*, J. Gómez-Laguna, L. Carrasco*, A. Saalmüller, T. Käser

Sources: Crossref, NC State University Libraries
Added: December 22, 2020

2013 journal article

Human Placental Alkaline Phosphatase as a Tracking Marker for Bone Marrow Mesenchymal Stem Cells

BioResearch Open Access, 2(5), 346–355.

By: E. Balmayor*, M. Flicker*, T. Käser*, A. Saalmüller* & R. Erben*

author keywords: model organisms for research; stem cells; tissue engineering
TL;DR: This study underscores the utility of genetically labeled BMSCs isolated from hPLAP-tg donors for long-term tracking of the fate of transplanted MSCs in regenerative therapies. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: July 20, 2019

2013 journal article

Immunohistochemical characterization of type II pneumocyte proliferation after challenge with type I porcine reproductive and respiratory syndrome virus

Journal of Comparative Pathology, 149(2-3), 322–330.

By: G. Balka*, A. Ladinig*, M. Ritzmann*, A. Saalmüller*, W. Gerner*, T. Käser*, C. Jakab*, M. Rusvai*, H. Weißenböck*

Contributors: G. Balka*, A. Ladinig*, M. Ritzmann*, A. Saalmüller*, W. Gerner*, T. Käser*, C. Jakab*, M. Rusvai*, H. Weißenböck*

author keywords: pig; PRRSV; TTF-1; type II pneumocyte
MeSH headings : Alveolar Epithelial Cells / metabolism; Alveolar Epithelial Cells / pathology; Animals; Enzyme-Linked Immunosorbent Assay; Immunohistochemistry; Porcine Reproductive and Respiratory Syndrome / metabolism; Porcine Reproductive and Respiratory Syndrome / pathology; Porcine respiratory and reproductive syndrome virus; Reverse Transcriptase Polymerase Chain Reaction; Swine
Source: ORCID
Added: February 11, 2019

2013 journal article

Phenotypic maturation of porcine NK- and T-cell subsets

Developmental and Comparative Immunology, 40(1), 51–68.

By: S. Talker*, T. Käser*, K. Reutner*, C. Sedlak*, K. Mair*, H. Koinig*, R. Graage*, M. Viehmann* ...

Contributors: S. Talker*, T. Käser*, K. Reutner*, C. Sedlak*, K. Mair*, H. Koinig*, R. Graage*, M. Viehmann* ...

author keywords: Swine; T-cell subsets; NK cells; Phenotypic maturation
MeSH headings : Animals; Animals, Newborn; Cell Differentiation; Immunologic Memory; Immunophenotyping; Killer Cells, Natural / immunology; Receptors, Antigen, T-Cell, gamma-delta / metabolism; Swine / immunology; T-Lymphocyte Subsets / immunology; T-Lymphocytes, Cytotoxic / immunology; T-Lymphocytes, Helper-Inducer / immunology; T-Lymphocytes, Regulatory / immunology
TL;DR: Major findings were: absolute numbers of γδ T cells strongly increased from birth until 19-25 weeks of age, indicating an important role for these cells during adolescence, and phenotype of T helper cells changed over time from CD8α(-)SLA-DR(-)CD27(+) towards CD8β(+)SLA(+)CD 27(-) but CD45RC(-) T helpers were found immediately after birth. (via Semantic Scholar)
Source: ORCID
Added: February 11, 2019

2012 journal article

Current knowledge on porcine regulatory T cells

Veterinary Immunology and Immunopathology, 148(1-2), 136–138.

By: T. Käser, W. Gerner, K. Mair, S. Hammer, M. Patzl & A. Saalmüller

Contributors: T. Käser, W. Gerner, K. Mair, S. Hammer, M. Patzl & A. Saalmüller

author keywords: Foxp3; Regulatory T cells; Tregs; Swine
MeSH headings : Animals; Forkhead Transcription Factors / immunology; Humans; Immune Tolerance / immunology; Swine / immunology; T-Lymphocytes, Regulatory / immunology
TL;DR: Porcine Tregs are known in humans and mice from last 15 years, and several studies led to a detailed knowledge on their phenotype, functions, and role in various immune reactions. (via Semantic Scholar)
Source: ORCID
Added: February 11, 2019

2012 journal article

Porcine T-helper and regulatory T cells exhibit versatile mRNA expression capabilities for cytokines and co-stimulatory molecules

Cytokine, 60(2), 400–409.

By: T. Käser, A. Müllebner, R. Hartl, S. Essler, A. Saalmüller & J. Catharina Duvigneau

Contributors: T. Käser, A. Müllebner, R. Hartl, S. Essler, A. Saalmüller & J. Catharina Duvigneau

author keywords: T-helper cells; Tregs; Cytokines; qPCR; Swine
MeSH headings : Animals; Antigens, CD / genetics; Antigens, CD / metabolism; Cytokines / genetics; Cytokines / metabolism; Flow Cytometry; Gene Expression Regulation; RNA, Messenger / metabolism; Sus scrofa / genetics; Sus scrofa / immunology; T-Lymphocytes, Helper-Inducer / metabolism; T-Lymphocytes, Regulatory / metabolism
TL;DR: Resting and activated TH cells showed an increased expression of various immune-modulatory molecules indicating that porcine TH cells possess distinct immunological skills in order to react on the actual immune situation. (via Semantic Scholar)
Source: ORCID
Added: February 11, 2019

2011 journal article

Porcine regulatory T cells: Mechanisms and T-cell targets of suppression

Developmental & Comparative Immunology, 35(11), 1166–1172.

author keywords: Foxp3; Regulatory T cells; Swine
MeSH headings : Animals; CD4-Positive T-Lymphocytes / immunology; Cell Communication; Forkhead Transcription Factors / biosynthesis; Forkhead Transcription Factors / metabolism; Immune Tolerance; Interleukin-10 / biosynthesis; Interleukin-10 / immunology; Interleukin-2 / biosynthesis; Interleukin-2 / immunology; Interleukin-2 / metabolism; Lymphocyte Activation; Receptors, Antigen, T-Cell, gamma-delta / immunology; Receptors, Antigen, T-Cell, gamma-delta / metabolism; Swine / immunology; T-Lymphocytes, Cytotoxic / immunology; T-Lymphocytes, Helper-Inducer / immunology; T-Lymphocytes, Helper-Inducer / metabolism; T-Lymphocytes, Regulatory / cytology; T-Lymphocytes, Regulatory / immunology; T-Lymphocytes, Regulatory / metabolism
TL;DR: Porcine Tregs can use all main mechanisms of suppression, and it was demonstrated that suppression affects T-helper cells as well as cytotoxic T lymphocytes and TCR-γδ T cells. (via Semantic Scholar)
Sources: ORCID, Crossref, NC State University Libraries
Added: February 11, 2019

2009 journal article

Characterization of CD79α+CD21− B cells in swine

Veterinary Immunology and Immunopathology, 128(1-3), 274.

UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: December 22, 2020

2009 journal article

Molecular characterisation of porcine Forkhead-box p3 (Foxp3)

Veterinary Immunology and Immunopathology, 132(2-4), 275–281.

Contributors: K. Bolzer*, T. Käser*, A. Saalmüller* & S. Hammer*

author keywords: Foxp3; Porcine; Sus scrofa; Swine; Regulatory T cells
MeSH headings : Amino Acid Sequence; Animals; Antibody Specificity; Base Sequence; Cattle; Cell Line; Cross Reactions; DNA Primers / genetics; DNA, Complementary / genetics; Forkhead Transcription Factors / chemistry; Forkhead Transcription Factors / genetics; Forkhead Transcription Factors / immunology; Humans; Mice; Rats; Recombinant Proteins / genetics; Recombinant Proteins / immunology; Sequence Homology, Amino Acid; Species Specificity; Swine / genetics; Swine / immunology; T-Lymphocytes, Regulatory / immunology; Transfection
TL;DR: This study represents the final proof for the specificity of the murine FJK-16s antibody for the porcine Foxp3 homologue and therefore strengthens future work on Porcine Tregs. (via Semantic Scholar)
Source: ORCID
Added: February 11, 2019

2009 journal article

Porcine T lymphocytes and NK cells - An update

Developmental and Comparative Immunology, 33(3), 310–320.

author keywords: Swine; T cells; NK cells
MeSH headings : Animals; Antigens, Differentiation / immunology; Antigens, Differentiation / metabolism; CD8 Antigens / immunology; CD8 Antigens / metabolism; Cytokines / metabolism; Genes, MHC Class II / immunology; Killer Cells, Natural / cytology; Killer Cells, Natural / immunology; Killer Cells, Natural / metabolism; Lymphocyte Activation; Natural Killer T-Cells / cytology; Natural Killer T-Cells / immunology; Natural Killer T-Cells / metabolism; Receptors, Antigen, T-Cell, alpha-beta / immunology; Receptors, Antigen, T-Cell, alpha-beta / metabolism; Receptors, Antigen, T-Cell, gamma-delta / immunology; Receptors, Antigen, T-Cell, gamma-delta / metabolism; Swine / immunology; T-Lymphocyte Subsets / cytology; T-Lymphocyte Subsets / immunology; T-Lymphocyte Subsets / metabolism
TL;DR: Current knowledge suggests that MHC class-I restricted cytolytic T cells can be identified by the expression of CD8alphabeta heterodimers, and first results indicate that these cells can suppress proliferation of other T cells and produce IL-10. (via Semantic Scholar)
Source: ORCID
Added: February 11, 2019

2008 journal article

Detection of Foxp3 protein expression in porcine T lymphocytes

Veterinary Immunology and Immunopathology, 125(1-2), 92–101.

author keywords: Foxp3; regulatory T cells; swine
MeSH headings : Animals; Antibodies, Monoclonal / chemistry; Antibodies, Monoclonal / immunology; Antibody Specificity; Blotting, Southern / veterinary; Blotting, Western / veterinary; CD4 Antigens / biosynthesis; CD4 Antigens / immunology; CD8 Antigens / biosynthesis; CD8 Antigens / immunology; Cross Reactions; Flow Cytometry / veterinary; Forkhead Transcription Factors / biosynthesis; Forkhead Transcription Factors / genetics; Interleukin-2 Receptor alpha Subunit / biosynthesis; Interleukin-2 Receptor alpha Subunit / immunology; RNA, Messenger / biosynthesis; RNA, Messenger / genetics; Reverse Transcriptase Polymerase Chain Reaction / veterinary; Swine / blood; Swine / immunology; T-Lymphocyte Subsets / immunology; T-Lymphocytes, Regulatory / immunology
TL;DR: This rat anti-mouse/rat Foxp3 antibody presents a powerful tool for the identification of porcine regulatory T cells as well as a reactivity tool for other species. (via Semantic Scholar)
Source: ORCID
Added: February 11, 2019

2008 journal article

Detection of intracellular antigens in porcine PBMC by flow cytometry: A comparison of fixation and permeabilisation reagents

Veterinary Immunology and Immunopathology, 121(3-4), 251–259.

Contributors: W. Gerner*, T. Käser*, M. Pintarič*, S. Groiß* & A. Saalmüller*

author keywords: intracellular antigen; swine; fixation; permeabilisation; flow cytometry
MeSH headings : Animals; CD79 Antigens / blood; CD79 Antigens / immunology; Flow Cytometry / methods; Flow Cytometry / veterinary; Interferon-gamma / blood; Interferon-gamma / immunology; Ki-67 Antigen / blood; Ki-67 Antigen / immunology; Leukocytes, Mononuclear / chemistry; Leukocytes, Mononuclear / immunology; Perforin / blood; Perforin / immunology; Swine / blood; Swine / immunology; Tissue Fixation / methods; Tissue Fixation / veterinary
TL;DR: A universal recommendation for a single fixation/permeabilisation strategy could not be deduced from the data but this work provides a useful guideline for optimal staining of common intracellular antigens analyzed in swine lymphocytes. (via Semantic Scholar)
Source: ORCID
Added: February 11, 2019

2008 journal article

Phenotypic and functional characterisation of porcine CD4+CD25high regulatory T cells

Veterinary Immunology and Immunopathology, 122(1-2), 153–158.

author keywords: regulatory T cells; swine; foxp3; T lymphocytes
MeSH headings : Animals; Forkhead Transcription Factors / analysis; Immunophenotyping; Interleukin-10 / biosynthesis; Swine / immunology; T-Lymphocytes, Regulatory / immunology; T-Lymphocytes, Regulatory / physiology
TL;DR: The results revealed that porcine CD4(+)CD25(+) T cells with high CD25 expression count for about 2-9% of the CD4-+) T-cell subset demonstrate a strong Foxp3 expression, a heterogeneous CD45RC-, CD8alpha- and MHC-II-defined phenotype and a moderate IL-10 production. (via Semantic Scholar)
Source: ORCID
Added: February 11, 2019

manuscript

A TriAdj-Adjuvanted <em>Chlamydia trachomatis </em>CPAF Protein Vaccine Is Highly Immunogenic in Pigs.

Tobias Kaeser

Source: ORCID
Added: March 15, 2024

journal article

Grouping Pig-Specific Responses to Mitogen with Similar Responder Animals may Facilitate the Interpretation of Results Obtained in an Out-Bred Animal Model

Pasternak, J. A., Hon Ng, S. L., Käser, T., Meurens, F., & Wilson, H. L. Journal of Vaccines and Vaccination, 5, 1–9.

By: J. Pasternak, S. Hon Ng, T. Käser, F. Meurens & H. Wilson

Source: NC State University Libraries
Added: July 11, 2019

Employment

Updated: September 20th, 2022 12:05

2016 - 2022

NC State University Raleigh, NC, US
Assistant Professor in Swine Immunology College of Veterinary Medicine - Population Health and Pathobiology

2013 - 2016

University of Saskatchewan Saskatoon, SK, CA
PostDoc (Establishment of the pig as a model to study chlamydia infections) Vaccine and Infectious Disease Organization (VIDO-InterVac)

2010 - 2013

University of Veterinary Medicine Vienna Vienna, AT
PostDoc (Role of Tregs in Porcine Reproductive and Respiratory Syndrome Virus infections) Institute of Immunology

Education

Updated: August 17th, 2016 11:00

2006 - 2010

University of Veterinary Medicine Vienna Vienna, AT
PhD (Immunology) Institute of Immunology

1999 - 2004

Eberhard Karls Universität Tübingen Tubingen, Baden-Württemberg, DE
Diploma in Biology (Molecular Biology, Virology, Microbiology)

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