Works (52)
Updated: July 15th, 2024 05:41
2024 journal article
Antihistamine Medication Blunts Localized-Vibration-Induced Increases in Popliteal Blood Flow
VIBRATION, 7(2), 351–361.
author keywords: percussion massage; Hypervolt; blood velocity
open access via www.mdpi.com (publisher PDF)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: July 8, 2024
2023 journal article
Low-dose intrapulmonary drug delivery device for studies on next-generation therapeutics in mice
Journal of Controlled Release, 359, 287–301.
Contributors: A. Gracioso Martins n, D. Snider n, K. Popowski n, K. Schuchard n, M. Tenorio n, S. Akunuri n, J. Wee n, K. Peters n
TL;DR:
A tunable nebulized system composed of an ultrasonic transducer equipped with a mesh nebulizer fitted with a silicone restrictor plate modification to control the nebulization rate provides targeted lung delivery efficiency far exceeding conventional nebulizers preventing waste of expensive biologics and large molecules during proof-of-concept and pre-clinical experiments involving mice.
(via Semantic Scholar)
Source: ORCID
Added: March 8, 2024
2023 journal article
The exon-skipping oligonucleotide, KitStop, depletes tissue-resident mast cells in vivo to ameliorate anaphylaxis
FRONTIERS IN IMMUNOLOGY, 14.
Contributors: B. Hedgespeth n, D. Snider n, K. Bitting n & n
author keywords: mast cell (MC); anaphylaxis; KIT; exon skipping; mast cell depletion
MeSH headings : Mice; Animals; Mast Cells; Anaphylaxis / genetics; Anaphylaxis / therapy; Mastocytosis; Allergens
TL;DR:
The use of an exon-skipping oligonucleotide is reported to safely reduce the severity and duration of the anaphylactic response via mast cell depopulation in tissues, resulting in a significant reduction in systemic mast cell responses.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: March 6, 2023
2022 article
Identification of redundancy between human Fc epsilon RI beta and MS4A6A proteins points toward additional complex mechanisms for Fc epsilon RI trafficking and signaling
Bitting, K., Hedgespeth, B., Ehrhardt-Humbert, L. C., Arthur, G. K., Schubert, A. G., Bradding, P., … Cruse, G. (2022, December 7). ALLERGY, Vol. 78.
Contributors: K. Bitting n, B. Hedgespeth n, L. Ehrhardt-Humbert n, G. Arthur n, A. Schubert n, P. Bradding *, S. Tilley*, n
author keywords: asthma; IgE; mast cells
MeSH headings : Animals; Humans; Mice; Basophils / metabolism; Cell Degranulation; Exons; Hypersensitivity / metabolism; Mast Cells / metabolism; Receptors, IgE / genetics; Receptors, IgE / metabolism; Signal Transduction
TL;DR:
It is reported that targeting FcεRIβ with exon‐skipping oligonucleotides eliminates IgE‐mediated degranulation in mouse MCs, but equivalent targeting in human MCs was not effective at reducing degranulations.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: November 25, 2022
2022 journal article
Immunologic and pathologic characterization of a novel swine biomedical research model for eosinophilic esophagitis
FRONTIERS IN ALLERGY, 3.
author keywords: pig; food allergy; animal model; immunology; gastrointestinal; eosinophilic esophagitis
TL;DR:
Swine is established as a relevant large animal model for translational biomedical research in EoE with the potential to facilitate development of therapeutics and it is demonstrated that the sensitization and oral challenge protocol not only induces the underlying immune markers but also the micro- and macro-pathological hallmarks of human EiE.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: September 25, 2023
2022 journal article
Regulation of Trafficking and Signaling of the High Affinity IgE Receptor by Fc epsilon RI beta and the Potential Impact of Fc epsilon RI beta Splicing in Allergic Inflammation
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 23(2).
Contributors: G. Arthur n & n
author keywords: mast cell; IgE receptor; Fc epsilon RI beta; antisense therapy; allergy; asthma; exon skipping
MeSH headings : Alternative Splicing; Animals; Biomarkers; Cell Degranulation / genetics; Cell Degranulation / immunology; Disease Susceptibility; Gene Expression Regulation; Humans; Hypersensitivity / diagnosis; Hypersensitivity / etiology; Hypersensitivity / metabolism; Hypersensitivity / therapy; Mast Cells / immunology; Mast Cells / metabolism; RNA Splicing; Receptors, IgE / chemistry; Receptors, IgE / genetics; Receptors, IgE / metabolism; Signal Transduction; Structure-Activity Relationship
TL;DR:
The characteristic role of FcεRIβ in both trafficking and signaling of the F cεRI receptor complex, the specificity to IgE-mediated activation pathways, and the preferential expression in mast cells and basophils makes Fc�RIβ an excellent, but challenging, candidate for therapeutic strategies in allergy and asthma, if targeting can be realized.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: February 7, 2022
2021 journal article
Identification of an ATP/P2X7/mast cell pathway mediating ozone-induced bronchial hyperresponsiveness
JCI INSIGHT, 6(21).
Contributors: X. Kong *, W. Bennett *, C. Jania*, K. Chason*, Z. German *, J. Adouli*, S. Budney*, B. Oby*
MeSH headings : Adenosine Triphosphate / metabolism; Animals; Bronchial Hyperreactivity / metabolism; Female; Humans; Mast Cells / metabolism; Mice; Ozone / adverse effects
TL;DR:
The data demonstrated that ozone induced substantial ATP release from human airway epithelia in vitro and into the airways of mice in vivo and that ATP served as a potent inducer of mast cell degranulation and BHR, acting through P2X7 receptors on mast cells.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: January 31, 2022
2021 journal article
Targeting KIT by frameshifting mRNA transcripts as a therapeutic strategy for aggressive mast cell neoplasms
MOLECULAR THERAPY, 30(1), 295–310.
Contributors: D. Snider n, G. Arthur n, G. Falduto *, A. Olivera *, L. Ehrhardt-Humbert n, E. Smith n, C. Smith n, D. Metcalfe *, n
MeSH headings : Humans; Mast Cells / metabolism; Mast Cells / pathology; Mastocytosis / genetics; Mastocytosis / pathology; Mastocytosis / therapy; Proto-Oncogene Proteins c-kit / genetics; RNA, Messenger / genetics; RNA, Messenger / metabolism; Receptor Protein-Tyrosine Kinases / metabolism
TL;DR:
It is proposed that the innovative approach, which employs well-tolerated, chemically stable oligonucleotides to target KIT expression through unconventional pathways, has potential as a KIT-targeted therapeutic alone, or in combination with agents thattarget KIT signaling, in the treatment of K IT-associated malignancies.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: February 7, 2022
2020 journal article
Dermal Extracellular Matrix-Derived Hydrogels as an In Vitro Substrate to Study Mast Cell Maturation
TISSUE ENGINEERING PART A, 27(15-16), 1008–1022.
Contributors: E. Ozpinar n , A. Frey n, G. Arthur n, C. Mora-Navarro n, A. Biehl n, D. Snider n, n , D. Freytes n
author keywords: extracellular matrix; mast cell; allergy; metabolism
MeSH headings : Animals; Cell Differentiation; Collagen; Extracellular Matrix; Humans; Hydrogels; Mast Cells; Swine
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: December 11, 2020
2020 journal article
Mast Cell-Biomaterial Interactions and Tissue Repair
TISSUE ENGINEERING PART B-REVIEWS, 27(6), 590–603.
Contributors: E. Ozpinar , A. Frey n, n & D. Freytes n
author keywords: mast cells; biomaterials; inflammation; tissue repair; foreign body response; wound healing
MeSH headings : Biocompatible Materials; Cell Communication; Fibrosis; Humans; Mast Cells / pathology; Tissue Engineering
TL;DR:
This review is designed for those in the tissue engineering and biomaterials fields who are interested in exploring the role MCs may play in wound-biomaterial interactions and wound healing and hopes to inspire more research in the MC-Biomaterial space in order to accelerate the design and construction of optimized implants.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: March 8, 2021
2020 journal article
Nanosilver composite pNIPAm microgels for the development of antimicrobial platelet‐like particles
Journal of Biomedical Materials Research Part B: Applied Biomaterials, 108(6), 2599–2609.
Contributors: E. Chee n, S. Nandi n, K. Nellenbach n, E. Mihalko n, D. Snider n, L. Morrill n, A. Bond n, E. Sproul n
author keywords: antimicrobial; microgels; nanosilver; platelet-mimetic; wound healing
MeSH headings : Acrylic Resins / chemistry; Animals; Anti-Infective Agents / chemistry; Anti-Infective Agents / pharmacology; Bacterial Infections / drug therapy; Bacterial Infections / microbiology; Blood Platelets; Clot Retraction; Colony-Forming Units Assay; Fibrin / chemistry; Fibrin / immunology; Gels; Hemorrhage / drug therapy; Hemostasis / drug effects; Liver / injuries; Male; Metal Nanoparticles; Mice; Mice, Inbred C57BL; Microgels; Silver / administration & dosage; Silver / chemistry; Wound Healing
TL;DR:
It is found that nanosilver incorporation does not affect base PLP performance while bestowing significant antimicrobial activity and enhancing infected wound healing outcomes, and Ag-PLPs have great promise for treating hemorrhage and improving healing following trauma.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: March 16, 2020
2020 journal article
Periostin Activation of Integrin Receptors on Sensory Neurons Induces Allergic Itch
CELL REPORTS, 31(1).
Contributors: S. Mishra n, J. Wheeler n, S. Pitake n, H. Ding *, C. Jiang *, T. Fukuyama n, J. Paps n, P. Ralph n
MeSH headings : Animals; Cell Adhesion Molecules / metabolism; Cell Adhesion Molecules / physiology; Dermatitis, Atopic / etiology; Dermatitis, Atopic / metabolism; Dermatitis, Atopic / pathology; Dogs; Female; Hypersensitivity / physiopathology; Integrin alpha5 / metabolism; Integrin beta3 / metabolism; Integrins / metabolism; Keratinocytes / metabolism; Male; Mice; Primates; Pruritus / metabolism; Pruritus / pathology; Sensory Receptor Cells / metabolism; Skin / metabolism
TL;DR:
A TSLP-periostin reciprocal activation loop that links the skin to the spinal cord via peripheral sensory neurons is identified and the non-canonical functional role of an integrin in itch is characterized.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: April 27, 2020
2020 journal article
The FcεRIβ homologue, MS4A4A, promotes FcεRI signal transduction and store-operated Ca2+ entry in human mast cells
Cellular Signalling, 71, 109617.
Contributors: G. Arthur n, L. Ehrhardt-Humbert n, D. Snider n, C. Jania*, S. Tilley*, D. Metcalfe *, n
author keywords: Mast cell; IgE receptor; MS4A; Allergy; Orai1
MeSH headings : Calcium / metabolism; Calcium Signaling; Cell Degranulation; Cell Line; Cholesterol / metabolism; Fetal Blood / metabolism; Humans; Mast Cells / metabolism; Mast Cells / physiology; Membrane Microdomains / metabolism; Membrane Proteins / metabolism; ORAI1 Protein / metabolism; Phospholipase C gamma / metabolism; Proto-Oncogene Proteins c-kit / metabolism; Receptors, IgE / metabolism; Sequence Homology, Amino Acid; Signal Transduction
TL;DR:
It is established that MS4A4A promotes phosphorylation of PLCγ1, calcium flux and degranulation in response to IgE-mediated crosslinking of FcεRI in human mast cells and thus represents a new target in the regulation of Ig E-mediated mast cell activation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: ORCID
Added: April 10, 2020
2019 article
The Periostin Activation of Integrin Receptors on Sensory Neurons Induces Allergic Itch
SSRN.
Contributors: S. Mishra n, J. Wheeler n, S. Pitake n, H. Ding *, C. Jiang, T. Fukuyama n, J. Paps n, P. Ralph n
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: March 8, 2024
2018 article
Exon Skipping of Fc epsilon RI beta for Allergic Diseases
EXON SKIPPING AND INCLUSION THERAPIES: METHODS AND PROTOCOLS, Vol. 1828, pp. 503–518.
Contributors: G. Arthur n & n
author keywords: Mast cell; Allergic diseases; Allergic inflammation; Fc epsilon RI; Exon skipping
MeSH headings : Anaphylaxis; Animals; Calcium / metabolism; Calcium Signaling; Cell Degranulation / immunology; Exons; Gene Expression Regulation; Genetic Predisposition to Disease; Humans; Hypersensitivity / genetics; Hypersensitivity / immunology; Mast Cells / immunology; Mast Cells / metabolism; Mice; Oligonucleotides, Antisense / administration & dosage; Oligonucleotides, Antisense / genetics; RNA Splicing; Receptors, IgE / genetics; Transfection
TL;DR:
The methods of exon skipping in mast cells and the assays used to examine the responses of mast cells in vitro and in vivo are described and a novel application to alter receptor trafficking and dampen allergic inflammation is demonstrated.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: March 4, 2019
2016 journal article
Constitutive KIT Activity and IL-6 Production in Mast Cells Alters Levels of Reactive Oxygen Species (ROS) and the Scavenger Protein DJ-1 in Mastocytosis
Journal of Allergy and Clinical Immunology, 137(2), AB281.
Sources: Crossref, NC State University Libraries
Added: January 25, 2020
2016 journal article
Exon skipping of Fc epsilon RI beta eliminates expression of the high-affinity IgE receptor in mast cells with therapeutic potential for allergy
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 113(49), 14115–14120.
Contributors: n , Y. Yin *, T. Fukuyama n, A. Desai*, G. Arthur n, W. Bäumer n, M. Beaven*, D. Metcalfe *
author keywords: mast cell; allergy; IgE receptor; oligonucleotides; dermatitis
MeSH headings : Animals; Calcium / metabolism; Cell Degranulation / drug effects; Cell Proliferation / drug effects; Cells, Cultured; Cytokines / biosynthesis; Dermatitis, Allergic Contact / therapy; Disease Models, Animal; Female; Humans; Mast Cells / drug effects; Mast Cells / metabolism; Mice, Inbred BALB C; Mice, Inbred C57BL; Oligonucleotides, Antisense / pharmacology; Oligonucleotides, Antisense / therapeutic use; Passive Cutaneous Anaphylaxis / genetics; RNA Splicing / drug effects; Receptors, IgE / genetics; Receptors, IgE / metabolism
TL;DR:
An innovative approach for targeting mast cells in vitro and in vivo is described using antisense oligonucleotide-mediated exon skipping of the β-subunit of the high-affinity IgE receptor (FcεRIβ) to eliminate surface high-Affinity Ig E receptor expression and function, rendering mast cells unresponsive to IgE-mediated activation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2016 journal article
Immunophenotypic and ultrastructural analysis of mast cells in hermansky-pudlak syndrome type-1: A possible connection to pulmonary fibrosis
PLoS ONE, 11(7).
Contributors: A. Kirshenbaum *, * , A. Desai*, G. Bandara *, M. Leerkes*, C. Lee *, E. Fischer *, . K.J. O'Brien
MeSH headings : Adult; Biomarkers; Case-Control Studies; Cell Line; Cells, Cultured; Chemotaxis; Cluster Analysis; Extracellular Matrix / metabolism; Female; Gene Expression Profiling; Hermanski-Pudlak Syndrome / diagnosis; Hermanski-Pudlak Syndrome / genetics; Hermanski-Pudlak Syndrome / metabolism; Humans; Imatinib Mesylate / pharmacology; Immunophenotyping; Lung / metabolism; Lung / pathology; Male; Mast Cells / drug effects; Mast Cells / metabolism; Mast Cells / ultrastructure; Membrane Proteins / genetics; Middle Aged; Mutation; Phenotype; Pulmonary Alveoli / metabolism; Pulmonary Alveoli / pathology; Pulmonary Fibrosis / etiology; Pulmonary Fibrosis / metabolism; Pulmonary Fibrosis / pathology; Young Adult
TL;DR:
The near-normalization of constitutive cytokine and matrix release following rescue by HPS1 transduction of HPM cells suggests that HPS-1 HuMCs may contribute to pulmonary fibrosis and constitute a target for therapeutic intervention.
(via Semantic Scholar)
Source: ORCID
Added: February 11, 2019
2016 journal article
Vibratory urticaria associated with a missense variant in ADGRE2
New England Journal of Medicine, 374(7), 656–663.
Contributors: S. Boyden *, A. Desai*, * , M. Young*, H. Bolan*, L. Scott *, A. Robin Eisch*, R. Daniel Long*
MeSH headings : Biopsy; Cell Degranulation / genetics; Female; Histamine / blood; Humans; Lebanon; Male; Mast Cells / physiology; Middle Aged; Mutation, Missense; Pedigree; Receptors, G-Protein-Coupled / genetics; Receptors, G-Protein-Coupled / metabolism; Skin / pathology; Urticaria / blood; Urticaria / etiology; Urticaria / genetics; Vibration / adverse effects
TL;DR:
It is shown that the previously unknown missense substitution in ADGRE2 probably destabilizes an autoinhibitory subunit interaction, sensitizing mast cells to IgE-independent vibration-induced degranulation, and cosegregating with vibratory urticaria in two large kindreds.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: February 11, 2019
2015 journal article
Constitutively Activated KIT in Mastocytosis Patients Is Associated with Decreased Levels of the Scavenger Protein DJ1 and Reciprocal Increases in Reactive Oxygen Species
Journal of Allergy and Clinical Immunology, 135(2), AB389.
Sources: Crossref, NC State University Libraries
Added: January 25, 2020
2015 journal article
Mast Cells Expressing the Germline HPS1 16-Bp Duplication (c.1470_1486dup16, Hermansky-Pudlak Syndrome-1) Defect Produce Extracellular Matrix Components
Journal of Allergy and Clinical Immunology, 135(2), AB172.
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
Sources: Crossref, NC State University Libraries
Added: January 25, 2020
2015 journal article
Mast cells in airway diseases and interstitial lung disease
European Journal of Pharmacology, 778, 125–138.
Contributors: * & P. Bradding *
author keywords: Mast cell; Asthma; Airway diseases; Interstitial lung disease
MeSH headings : Animals; Cell Proliferation; Humans; Lung Diseases, Interstitial / immunology; Lung Diseases, Interstitial / microbiology; Mast Cells / microbiology; Mast Cells / pathology
TL;DR:
The role that mast cells play in airway diseases is discussed and how mast cell microlocalisation within specific lung compartments and their cellular interactions are likely to be critical for their effector function in disease are highlighted.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: February 11, 2019
2015 journal article
The CD20 homologue MS4A4 directs trafficking of KIT toward clathrin-independent endocytosis pathways and thus regulates receptor signaling and recycling
Molecular Biology of the Cell, 26(9), 1711–1727.
Contributors: * , M. Beaven*, S. Music*, P. Bradding *, A. Gilfillan* & D. Metcalfe *
MeSH headings : Antigens, CD20 / physiology; Cell Line; Cell Membrane / metabolism; Clathrin / metabolism; Endocytosis; Endosomes / metabolism; Gene Expression; Humans; Mast Cells / metabolism; Membrane Proteins / physiology; Phospholipase C gamma / metabolism; Phosphorylation; Protein Processing, Post-Translational; Protein Transport; Proto-Oncogene Proteins c-kit / metabolism; Signal Transduction; rab5 GTP-Binding Proteins / metabolism
TL;DR:
This study is the first to describe functions for human MS4A4, which reduces KIT recruitment to lipid raft microdomains and PLCg1 signaling while promoting AKT signaling, cell migration, and proliferation.
(via Semantic Scholar)
Source: ORCID
Added: February 11, 2019
2015 journal article
The Fcεriβ Homologue, MS4A4, Promotes Fcεri-Dependent Human Mast Cell Degranulation By Facilitating PLCγ1 Signaling
Journal of Allergy and Clinical Immunology, 135(2), AB240.
Sources: Crossref, NC State University Libraries
Added: January 25, 2020
2014 book
Flow cytometry-based monitoring of mast cell activation
In Methods in Molecular Biology (Vol. 1220, pp. 365–379).
Contributors: * , A. Gilfillan* & D. Smrz *
author keywords: Mast cell; Signaling; Protein phosphorylation; Calcium signal; Actin polymerization/depolymerization; Degranulation; Flow cytometry
TL;DR:
Flow-cytometry-based assays are described that can be utilized to examine signaling processes and degranulation in both pure mast cell populations and, following appropriate selection, in populations where the mast cells of interest may only represent a fraction of the total cell population.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: February 11, 2019
2014 journal article
Functional deregulation of KIT: Link to mast cell proliferative diseases and other neoplasms
Immunology and Allergy Clinics of North America, 34(2), 219–237.
Contributors: * , D. Metcalfe * & A. Olivera *
author keywords: Mastocytosis; KIT mutations; KIT signaling; KIT trafficking; KIT inhibitors
MeSH headings : Bone Marrow / drug effects; Bone Marrow / metabolism; Bone Marrow / pathology; Cell Proliferation; Exons; Gene Expression Regulation, Neoplastic; Hematologic Neoplasms / diagnosis; Hematologic Neoplasms / drug therapy; Hematologic Neoplasms / genetics; Hematologic Neoplasms / pathology; Humans; Mast Cells / drug effects; Mast Cells / metabolism; Mast Cells / pathology; Mastocytosis / diagnosis; Mastocytosis / drug therapy; Mastocytosis / genetics; Mastocytosis / pathology; Mutation; Protein Kinase Inhibitors / therapeutic use; Protein Structure, Tertiary; Protein Transport; Proto-Oncogene Proteins c-kit / genetics; Proto-Oncogene Proteins c-kit / metabolism; Signal Transduction / genetics; Stem Cell Factor / genetics; Stem Cell Factor / metabolism
TL;DR:
The authors discuss common gain-of-function mutations in the stem cell factor receptor KIT found in mast cell proliferation disorders and summarize the current understanding of the molecular mechanisms by which these transforming mutations may affect KIT structure and function leading to altered downstream signaling and cellular transformation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: February 11, 2019
2014 journal article
Rictor negatively regulates high-affinity receptors for IgE-induced mast cell degranulation
Journal of Immunology, 193(12), 5924–5932.
Contributors: D. Smrz *, * , M. Beaven*, A. Kirshenbaum *, D. Metcalfe * & A. Gilfillan*
MeSH headings : Actins / metabolism; Calcium Signaling; Carrier Proteins / genetics; Carrier Proteins / metabolism; Cell Degranulation / genetics; Cell Degranulation / immunology; Cell Line; Enzyme Activation; Gene Knockdown Techniques; Humans; Mast Cells / immunology; Mast Cells / metabolism; Protein Aggregates; Protein Transport; RNA Interference; Rapamycin-Insensitive Companion of mTOR Protein; Receptors, IgE / metabolism; Signal Transduction; TOR Serine-Threonine Kinases / metabolism
TL;DR:
Evidence is provided that rictor is a multifunctional signaling regulator that can regulate FcεRI-mediated degranulation independently of mTORC2.
(via Semantic Scholar)
Source: ORCID
Added: February 11, 2019
2013 journal article
A Truncated Splice-Variant of the FcεRIβ Receptor Subunit Is Critical for Microtubule Formation and Degranulation in Mast Cells
Immunity, 38(5), 906–917.
Contributors: * , M. Beaven*, I. Ashmole*, P. Bradding *, A. Gilfillan* & D. Metcalfe *
MeSH headings : Adaptor Proteins, Signal Transducing / metabolism; Calcium / metabolism; Calcium Signaling / immunology; Calmodulin-Binding Proteins / genetics; Calmodulin-Binding Proteins / immunology; Cell Degranulation / immunology; Golgi Apparatus / metabolism; Humans; Hypersensitivity / immunology; Immunoglobulin E / immunology; Interleukin-8 / metabolism; Mast Cells / immunology; Mast Cells / metabolism; Microtubules / metabolism; Prostaglandin D2 / biosynthesis; Prostaglandin D2 / immunology; Protein Isoforms / immunology; RNA Interference; RNA Splicing; RNA, Messenger; RNA, Small Interfering; Receptors, IgE / genetics; Receptors, IgE / metabolism
TL;DR:
It is determined that t-FcεRIβ is critical for microtubule formation and degranulation and that it may perform this function by trafficking adaptor molecules and kinases to the pericentrosomal and Golgi region in response to Ca2+ signals.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: February 11, 2019
2013 journal article
Diminished allergic disease in patients with STAT3 mutations reveals a role for STAT3 signaling in mast cell degranulation
Journal of Allergy and Clinical Immunology, 132(6).
MeSH headings : Adolescent; Adult; Aged; Animals; Cell Degranulation / genetics; Cells, Cultured; Child; Child, Preschool; Dermatitis, Atopic / genetics; Dermatitis, Atopic / immunology; Female; Food Hypersensitivity / epidemiology; Food Hypersensitivity / genetics; Food Hypersensitivity / immunology; Humans; Immunoglobulin E / metabolism; Incidence; Infant; Job Syndrome / epidemiology; Job Syndrome / genetics; Job Syndrome / immunology; Male; Mast Cells / immunology; Mice; Mice, Inbred C57BL; Mice, Transgenic; Middle Aged; Mutation / genetics; STAT3 Transcription Factor / genetics; STAT3 Transcription Factor / physiology; Signal Transduction / genetics; Transgenes / genetics; Young Adult
Source: ORCID
Added: February 11, 2019
2013 journal article
Diminished allergic disease in patients with STAT3 mutations reveals a role for STAT3 signaling in mast cell degranulation
Journal of Allergy and Clinical Immunology, 132(6), 1388–1396.e3.
author keywords: Autosomal dominant hyper-IgE syndrome; Job syndrome; signal transducer and activator of transcription 3; mast cell; degranulation
MeSH headings : Adolescent; Adult; Aged; Animals; Cell Degranulation / genetics; Cells, Cultured; Child; Child, Preschool; Dermatitis, Atopic / genetics; Dermatitis, Atopic / immunology; Female; Food Hypersensitivity / epidemiology; Food Hypersensitivity / genetics; Food Hypersensitivity / immunology; Humans; Immunoglobulin E / metabolism; Incidence; Infant; Job Syndrome / epidemiology; Job Syndrome / genetics; Job Syndrome / immunology; Male; Mast Cells / immunology; Mice; Mice, Inbred C57BL; Mice, Transgenic; Middle Aged; Mutation / genetics; STAT3 Transcription Factor / genetics; STAT3 Transcription Factor / physiology; Signal Transduction / genetics; Transgenes / genetics; Young Adult
TL;DR:
This study serves as an example for how mutations in specific atopic pathways can lead to discrete allergic phenotypes, encompassing increased risk of some phenotypes but a relative protection from others.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 5, 2020
2013 journal article
Establishment of the Hermansky Pudlak Mastocyte (HPM) Cell Line Which Has the HPS1 16-Bp Duplication (c.1470_1486dup16)
Journal of Allergy and Clinical Immunology, 131(2), AB115.
Sources: Crossref, NC State University Libraries
Added: February 5, 2020
2012 journal article
Cold urticaria, immunodeficiency, and autoimmunity related to PLCG2 deletions
New England Journal of Medicine, 366(4), 330–338.
Contributors: M. Ombrello *, E. Remmers *, G. Sun *, A. Freeman *, S. Datta*, P. Torabi-Parizi *, N. Subramanian *, T. Bunney *
MeSH headings : Autoimmune Diseases / genetics; Cold Temperature / adverse effects; Cryopyrin-Associated Periodic Syndromes / genetics; DNA, Complementary / analysis; DNA, Complementary / isolation & purification; Female; Humans; Immunologic Deficiency Syndromes / genetics; Male; Pedigree; Phenotype; Phospholipase C gamma / genetics; Phospholipase C gamma / metabolism; Polymorphism, Single Nucleotide; Sequence Analysis, DNA; Sequence Deletion
TL;DR:
Genomic deletions in PLCG2 cause gain of PLCγ(2) function, leading to signaling abnormalities in multiple leukocyte subsets and a phenotype encompassing both excessive and deficient immune function.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
Source: ORCID
Added: February 11, 2019
2011 journal article
Functional K<inf>Ca</inf>3.1 K<sup>+</sup> channels are required for human fibrocyte migration
Journal of Allergy and Clinical Immunology, 128(6).
Contributors: * , S. Singh *, S. Duffy*, C. Doe*, R. Saunders *, C. Brightling *, P. Bradding *
author keywords: Pulmonary fibrosis; asthma; fibrocyte; cell migration; ion channel; K(Ca)3.1; patch clamp electrophysiology
MeSH headings : Airway Remodeling / physiology; Asthma / metabolism; Asthma / pathology; Blotting, Western; Cell Differentiation / physiology; Cell Movement; Connective Tissue Cells / cytology; Connective Tissue Cells / metabolism; Fibrosis / metabolism; Fibrosis / pathology; Humans; Intermediate-Conductance Calcium-Activated Potassium Channels / metabolism; Patch-Clamp Techniques; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction
TL;DR:
The K+ channel KCa3.1 plays a key role in human fibrocyte migration and might attenuate tissue fibrosis and remodeling in patients with diseases such as asthma and idiopathic pulmonary fibrosis through the inhibition of fibroCyte recruitment.
(via Semantic Scholar)
Source: ORCID
Added: February 11, 2019
2010 journal article
A novel FcεRIβ-chain truncation regulates human mast cell proliferation and survival
FASEB Journal, 24(10), 4047–4057.
Contributors: * , D. Kaur *, M. Leyland* & P. Bradding *
author keywords: MS4A2; IgE receptor; cell cycle; G2 phase; cell membrane
MeSH headings : Amino Acid Sequence; Apoptosis; Base Sequence; Blotting, Western; Cell Cycle; Cell Proliferation; Cell Survival / physiology; DNA; DNA Primers; Electrophoresis, Polyacrylamide Gel; Humans; Mast Cells / cytology; Molecular Sequence Data; Receptors, IgE / chemistry; Receptors, IgE / physiology; Reverse Transcriptase Polymerase Chain Reaction
TL;DR:
This finding demonstrates that the MS4A2 gene has multiple roles, extending beyond the regulation of acute allergic responses, and might be possible to induce its expression in mast cells in vivo, which could lead to better treatments for diseases such as mastocytosis and asthma.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: February 11, 2019
2010 journal article
Activation of human mast cells through the platelet-activating factor receptor
Journal of Allergy and Clinical Immunology, 125(5).
Contributors: N. Kajiwara*, T. Sasaki*, P. Bradding *, * , H. Sagara *, K. Ohmori*, H. Saito *, C. Ra*, Y. Okayama *
author keywords: Anaphylaxis; histamine; human lung mast cells; human peripheral blood-derived mast cells; human skin mast cells; platelet-activating factor receptor
MeSH headings : Anaphylaxis / immunology; Anaphylaxis / physiopathology; Cells, Cultured; Histamine Release; Humans; Lung / cytology; Mast Cells / cytology; Mast Cells / immunology; Mast Cells / metabolism; Platelet Activating Factor / metabolism; Platelet Membrane Glycoproteins / metabolism; Receptors, G-Protein-Coupled / metabolism; Reverse Transcriptase Polymerase Chain Reaction; Signal Transduction; Skin / cytology
TL;DR:
The findings provide a mechanism whereby PAF mediates an amplification loop for MC activation in the generation of anaphylaxis, and it is shown for the first time that PAF induces histamine release from human lung MCs and PB-derived MCs but not skin MCs.
(via Semantic Scholar)
Source: ORCID
Added: February 11, 2019
2010 journal article
Airway smooth muscle proliferation and survival is not modulated by mast cells
Clinical and Experimental Allergy, 40(2), 279–288.
Contributors: D. Kaur *, F. Hollins*, R. Saunders *, L. Woodman*, A. Sutcliffe *, * , P. Bradding *, C. Brightling *
author keywords: airway smooth muscle; apoptosis; asthma; mast cells; necrosis; proliferation; survival
MeSH headings : Aged; Apoptosis; Asthma / immunology; Asthma / pathology; Cell Proliferation; Cell Survival; Cells, Cultured; Coculture Techniques; Female; Humans; Immunoglobulin E / immunology; Male; Mast Cells / immunology; Mast Cells / physiology; Middle Aged; Muscle, Smooth / metabolism; Muscle, Smooth / pathology
TL;DR:
Background Airway smooth muscle (ASM) hyperplasia and mast cell localization within the ASM bundle are important features of asthma and the cause of this increased ASM mass is uncertain.
(via Semantic Scholar)
Source: ORCID
Added: February 11, 2019
2010 journal article
Human lung mast cells mediate pneumococcal cell death in response to activation by pneumolysin
Journal of Immunology, 184(12), 7108–7115.
Contributors: * , V. Fernandes *, J. De Salort*, D. Pankhania*, M. Marinas*, H. Brewin*, P. Andrew *, P. Bradding *, A. Kadioglu *
MeSH headings : Bacterial Proteins / immunology; Cell Degranulation / immunology; Cells, Cultured; Coculture Techniques; Humans; Lung / cytology; Lung / immunology; Mast Cells / immunology; Mast Cells / microbiology; Pneumococcal Infections / immunology; Streptococcus pneumoniae / immunology; Streptolysins / immunology
TL;DR:
HLMCs exhibit direct antimicrobial activity to pneumococci through their activation by pneumolysin, and the release of LL-37 from HLMCs suggests that mast cells provide an early warning system and potentially limit pneumococcal dissemination early in the course of invasive pulmonary pneumitiscal disease.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: February 11, 2019
2009 journal article
Airway wall geometry in asthma and nonasthmatic eosinophilic bronchitis
Allergy: European Journal of Allergy and Clinical Immunology, 64(6), 951–958.
Contributors: S. Siddiqui *, S. Gupta *, * , P. Haldar *, J. Entwisle*, S. McDonald, P. Whithers, S. Hainsworth *, H. Coxson *, C. Brightling *
author keywords: airway hyperresponsiveness; asthma; computed tomography; eosinophilic bronchitis; geometry
MeSH headings : Adult; Aged; Asthma / pathology; Bronchi / pathology; Bronchial Hyperreactivity / pathology; Bronchitis / pathology; Eosinophilia / pathology; Female; Humans; Male; Middle Aged; Tomography, X-Ray Computed
TL;DR:
Variable airflow obstruction and airway hyperresponsiveness are features of asthma, which are absent in nonasthmatic eosinophilic bronchitis, but whether the airway geometry differs between asthma and non asthmatic EB is uncertain.
(via Semantic Scholar)
Source: ORCID
Added: February 11, 2019
2009 journal article
Counterregulation of β<inf>2</inf>-adrenoceptor function in human mast cells by stem cell factor
Journal of Allergy and Clinical Immunology, 125(1-3).
Contributors: * , W. Yang*, S. Duffy*, L. Chachi*, M. Leyland*, Y. Amrani *, P. Bradding *
author keywords: beta(2)-Adrenoceptor; albuterol; asthma; mast cell; desensitization
MeSH headings : Albuterol / metabolism; Albuterol / pharmacology; Asthma / drug therapy; Asthma / metabolism; Asthma / physiopathology; Cell Line; Histamine Release; Humans; Lung / cytology; Lung / drug effects; Mast Cells / drug effects; Mast Cells / immunology; Mast Cells / metabolism; Potassium Channels, Calcium-Activated / metabolism; Receptors, Adrenergic, beta-2 / drug effects; Receptors, Adrenergic, beta-2 / metabolism; Stem Cell Factor / metabolism; Stem Cell Factor / pharmacology
TL;DR:
Albuterol actually potentiated IgE-induced histamine release in a dose-dependent manner when both SCF and IgE were present, suggesting the potentially beneficial effects of beta(2)-adrenoceptor agonists in asthmatic airways may be blunted as a result of the high concentrations of SCF present.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: February 11, 2019
2009 journal article
IL-13 expression by blood T cells and not eosinophils is increased in asthma compared to non-asthmatic eosinophilic bronchitis
BMC Pulmonary Medicine, 9.
Contributors: S. Siddiqui *, * , S. Mckenna*, W. Monteiro*, V. Mistry *, A. Wardlaw *, C. Brightling *
MeSH headings : Adult; Asthma / blood; Asthma / pathology; Bronchitis / blood; Bronchitis / pathology; Case-Control Studies; Eosinophils / metabolism; Eosinophils / pathology; Female; Humans; Interleukin-13 / blood; Leukocytes, Mononuclear / metabolism; Leukocytes, Mononuclear / pathology; Male; Middle Aged; T-Lymphocytes / metabolism; T-Lymphocytes / pathology; Up-Regulation
Source: ORCID
Added: February 11, 2019
2008 journal article
Engagement of the EP<inf>2</inf> prostanoid receptor closes the K<sup>+</sup> channel K<inf>Ca</inf>3.1 in human lung mast cells and attenuates their migration
European Journal of Immunology, 38(9), 2548–2556.
Contributors: S. Duffy*, * , S. Cockerill*, C. Brightling * & P. Bradding *
author keywords: chemotaxis; ion channel; K(Ca)3.1; mast cell; prostaglandin E-2
MeSH headings : Alprostadil / analogs & derivatives; Alprostadil / pharmacology; Benzimidazoles / pharmacology; Calcium Channel Agonists / pharmacology; Cell Movement / drug effects; Chemotaxis; Dinoprostone / metabolism; Histamine Release; Humans; Mast Cells / drug effects; Mast Cells / immunology; Mast Cells / physiology; Potassium Channels / immunology; Potassium Channels / metabolism; Prostaglandin Antagonists / pharmacology; Receptors, Prostaglandin E / agonists; Receptors, Prostaglandin E / antagonists & inhibitors; Receptors, Prostaglandin E / metabolism; Receptors, Prostaglandin E, EP2 Subtype; Xanthones / pharmacology
TL;DR:
It is shown that the Gs‐coupled EP2 receptor engagement closes KCa3.1 in HLMC and attenuates both chemokine‐ and PGE2‐dependent HLMC migration, which may be useful for the treatment of mast cell‐mediated disease.
(via Semantic Scholar)
Source: ORCID
Added: February 11, 2019
2008 journal article
Human airway smooth muscle promotes human lung mast cell survival, proliferation, and constitutive activation: Cooperative roles for CADM1, stem cell factor, and IL-6
Journal of Immunology, 181(4), 2772–2780.
Contributors: F. Hollins*, D. Kaur *, W. Yang*, * , R. Saunders *, A. Sutcliffe *, P. Berger *, A. Ito *, C. Brightling *, P. Bradding *
MeSH headings : Cell Adhesion / immunology; Cell Adhesion Molecule-1; Cell Adhesion Molecules; Cell Communication / immunology; Cell Movement / immunology; Cell Proliferation; Cell Survival / immunology; Cells, Cultured; Coculture Techniques; Humans; Immunoglobulins / physiology; Interleukin-6 / physiology; Mast Cells / cytology; Mast Cells / immunology; Mast Cells / metabolism; Membrane Proteins / physiology; Muscle, Smooth / cytology; Muscle, Smooth / immunology; Respiratory Mucosa / cytology; Respiratory Mucosa / immunology; Respiratory Mucosa / metabolism; Stem Cell Factor / physiology; Tumor Suppressor Proteins / physiology
TL;DR:
It is shown that human ASM maintains human lung mast cell (HLMC) survival in vitro and induces rapid HLMC proliferation and a novel smooth muscle-driven allergen-independent mechanism of chronic mast cell activation is revealed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: February 11, 2019
2008 journal article
IgE alone promotes human lung mast cell survival through the autocrine production of IL-6
BMC Immunology, 9.
Contributors: * , S. Cockerill* & P. Bradding *
MeSH headings : Autocrine Communication; Benzothiazoles; Cell Separation; Cell Survival; Cells, Cultured; Diamines; Enzyme-Linked Immunosorbent Assay; Gene Expression Regulation; Humans; Immunoglobulin E / immunology; Interleukin-6 / biosynthesis; Interleukin-6 / genetics; Interleukin-6 / immunology; Interleukin-6 / metabolism; Lung / cytology; Lung / immunology; Mast Cells / cytology; Mast Cells / immunology; Nucleic Acid Denaturation; Organic Chemicals; Quinolines; RNA, Messenger / genetics; RNA, Messenger / metabolism; Reverse Transcriptase Polymerase Chain Reaction; Subcellular Fractions; Transcription, Genetic
TL;DR:
It is suggested that IgE in the absence of antigen promotes HLMC survival through the autocrine production of IL-6 through which anti-IgE therapy might achieve its therapeutic effect.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: February 11, 2019
2008 chapter
Mast Cells: Biological Properties and Role in Health and Allergic Diseases
In Allergy and Allergic Diseases, Second Edition (Vol. 1, pp. 217–257).
Contributors: P. Bradding * & *
Source: ORCID
Added: February 11, 2019
2008 journal article
Mast cells promote airway smooth muscle cell differentiation via autocrine up-regulation of TGF-β1
Journal of Immunology, 181(7), 5001–5007. http://www.scopus.com/inward/record.url?eid=2-s2.0-58149280835&partnerID=MN8TOARS
Source: ORCID
Added: February 11, 2019
2007 journal article
Adenosine closes the K<sup>+</sup> channel K<inf>Ca</inf>3.1 in human lung mast cells and inhibits their migration via the adenosine A<inf>2A</inf> receptor
European Journal of Immunology, 37(6), 1653–1662.
Contributors: S. Duffy*, * , C. Brightling * & P. Bradding *
author keywords: adenosine; chemotaxis; ion channel K(Ca)3.1; mast cell
MeSH headings : Adenosine / analogs & derivatives; Adenosine / pharmacology; Adenosine A2 Receptor Agonists; Adenosine A2 Receptor Antagonists; Benzimidazoles / pharmacology; Calcium Channel Agonists / pharmacology; Cations / metabolism; Cell Movement / drug effects; Culture Media, Conditioned / pharmacology; Electric Stimulation; Electrophysiology; Histamine / metabolism; Humans; Immunoglobulin E / pharmacology; Intermediate-Conductance Calcium-Activated Potassium Channels / metabolism; Lung / cytology; Mast Cells / drug effects; Mast Cells / metabolism; Mast Cells / physiology; Phenethylamines / pharmacology; Receptor, Adenosine A2A / metabolism; Receptor, Adenosine A2B / metabolism; Triazines / pharmacology; Triazoles / pharmacology
TL;DR:
The Gαs‐coupledAdenosine A2A receptor closes KCa3.1, providing a clearly defined mechanism by which adenosine inhibits HLMC migration and degranulation, and may be useful for the treatment of mast cell‐mediated disease.
(via Semantic Scholar)
Source: ORCID
Added: February 11, 2019
2007 journal article
K<inf>Ca</inf>3.1 Ca<sup>2+</sup>-activated K<sup>+</sup> channels regulate human airway smooth muscle proliferation
American Journal of Respiratory Cell and Molecular Biology, 37(5), 525–531.
author keywords: asthma; ion channels; cell cycle
MeSH headings : Calcium / physiology; Cell Proliferation; Cells, Cultured; Humans; Intermediate-Conductance Calcium-Activated Potassium Channels / biosynthesis; Intermediate-Conductance Calcium-Activated Potassium Channels / genetics; Intermediate-Conductance Calcium-Activated Potassium Channels / physiology; Muscle, Smooth / cytology; Muscle, Smooth / metabolism; Potassium / metabolism; RNA, Messenger / metabolism; Respiratory System / cytology; Respiratory System / metabolism; Transforming Growth Factor beta / physiology; Up-Regulation / physiology
TL;DR:
It is demonstrated, for the first time, that K(Ca)3.1 channels are important in HASM cell proliferation, making these channels a potential therapeutic target in airway remodeling.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
Source: ORCID
Added: February 11, 2019
2006 journal article
Functional K<inf>Ca</inf>3.1 K<sup>+</sup> channels are required for human lung mast cell migration
Thorax, 61(10), 880–885.
MeSH headings : Asthma / metabolism; Asthma / pathology; Calcium Channel Blockers / pharmacology; Carcinoma, Bronchogenic / pathology; Cell Proliferation; Cells, Cultured; Chemotaxis / physiology; Clotrimazole / pharmacology; Cytokines / pharmacology; Humans; Lung / cytology; Lung / metabolism; Mast Cells / cytology; Mast Cells / metabolism; Potassium Channels, Calcium-Activated / metabolism
TL;DR:
Since modulation of KCa3.1 can inhibit HLMC chemotaxis to diverse chemoattractant stem cell factor, and the supernatants from tumour necrosis factor α stimulated asthmatic ASM was markedly inhibited with both the non-selective KCa 3.1 blocker charybdotoxin and the highly specific TRAM-34 in a dose dependent manner.
(via Semantic Scholar)
Source: ORCID
Added: February 11, 2019
2005 journal article
Activation of human lung mast cells by monomeric immunoglobulin E
European Respiratory Journal, 25(5), 858–863.
author keywords: asthma; calcium; human; mast cell; mediators; monomeric immunoglobulin E
MeSH headings : Calcium / metabolism; Cells, Cultured; Culture Media, Conditioned; Histamine / metabolism; Humans; Immunoglobulin E / metabolism; Interleukin-8 / metabolism; Leukotriene C4 / metabolism; Lung / cytology; Lung / immunology; Mast Cells / immunology; Mast Cells / metabolism; Reference Values; Stem Cell Factor
TL;DR:
The hypothesis that immunoglobulin E loading of mast cells within the asthmatic airway contributes to the disordered airway physiology of this disease is supported.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: February 11, 2019
2005 journal article
Activation of human lung mast cells by monomeric immunoglobulin E (multiple letters) [2]
European Respiratory Journal, 26(4), 744–746.
Contributors: S. Hart *, I. Dransfield, P. Bradding &
MeSH headings : Centrifugation; Chromatography; Humans; Immunoglobulin E / chemistry; Immunoglobulin E / immunology; Immunoglobulin E / pharmacology; Immunologic Factors / chemistry; Immunologic Factors / immunology; Immunologic Factors / pharmacology; Lung / immunology; Mast Cells / drug effects; Mast Cells / immunology
TL;DR:
Immunoglobulin preparations contain variable proportions of Ig aggregates 1, which behave like true immune complexes and are able to activate inflammatory cells by crosslinking Fc receptors 2.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: February 11, 2019
2005 journal article
β<inf>2</inf>-adrenoceptor regulation of the K<sup>+</sup> channel iK<inf>Ca</inf>1 in human mast cells
FASEB Journal, 19(8), 1006–1008.
Contributors: S. Duffy*, * , W. Lawley* & P. Bradding *
author keywords: salbutamol; ICI 118551; K(Ca)3.1; lung; G protein
MeSH headings : Adrenergic beta-Agonists / pharmacology; Albuterol / pharmacology; Antibodies / pharmacology; Benzimidazoles / pharmacology; Cells, Cultured; Electric Conductivity; GTP-Binding Protein alpha Subunits, Gs; Humans; Immunoglobulin E / immunology; Immunoglobulin E / physiology; Intermediate-Conductance Calcium-Activated Potassium Channels / drug effects; Intermediate-Conductance Calcium-Activated Potassium Channels / physiology; Ion Channel Gating / drug effects; Lung / cytology; Mast Cells / chemistry; Mast Cells / drug effects; Mast Cells / physiology; Propanolamines / pharmacology; Receptors, Adrenergic, beta-2 / physiology
TL;DR:
This is the first demonstration that gating of the iKCa1 channel is regulated by a G protein‐coupled receptor and provides a clearly defined mechanism for the mast cell “stabilizing” effect of β2‐agonists.
(via Semantic Scholar)
Source: ORCID
Added: February 11, 2019
2004 journal article
The K <sup>+</sup> channel iK <inf>CA</inf>1 potentiates Ca <sup>2+</sup> influx and degranulation in human lung mast cells
Journal of Allergy and Clinical Immunology, 114(1), 66–72.
Contributors: S. Mark Duffy*, P. Berger *, * , W. Yang*, S. Bolton * & P. Bradding *
author keywords: mast cell; human; K+; ion channel; Ca2+; histamine
MeSH headings : Benzimidazoles / pharmacology; Calcium / metabolism; Calcium Channel Agonists / pharmacology; Calcium Channel Blockers / pharmacology; Cell Degranulation / drug effects; Cell Degranulation / immunology; Cells, Cultured; Clotrimazole / pharmacology; Histamine / immunology; Humans; Immunoglobulin E / immunology; Intermediate-Conductance Calcium-Activated Potassium Channels; Lung / cytology; Lung / immunology; Mast Cells / drug effects; Mast Cells / immunology; Patch-Clamp Techniques; Potassium Channels / drug effects; Potassium Channels / metabolism; Pyrazoles / pharmacology
TL;DR:
Opening of iKCA1 enhances IgE-dependent Ca2+ influx and histamine release in HLMCs and may provide a novel approach to the treatment of mast cell-mediated disease.
(via Semantic Scholar)
Source: ORCID
Added: February 11, 2019
Employment
Updated: March 7th, 2024 14:24
2023 - present
North Carolina State University Raleigh, US
Associate Professor
Molecular Biomedical Sciences
2016 - 2023
NC State University Raleigh, NC, US
Assistant Professor
Molecular Biomedical Sciences
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