Donald Osvaldo Freytes

Works (46)

Updated: April 5th, 2024 09:19

2023 article

Characterization and biological evaluation of a novel flavonoid-collagen antioxidant hydrogel with cytoprotective properties

Nalband, D. M., Sarker, P., Khan, S. A., & Freytes, D. O. (2023, September 16). JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, Vol. 9.

By: D. Nalband n, P. Sarker n, S. Khan n  & D. Freytes n

author keywords: antioxidant; collagen; hydrogel; luteolin; reactive oxygen species; skin graft; wound healing
TL;DR: Adequate cell viability and metabolic activity of dermal fibroblasts cultured within the gels were measured across all formulations, resulting in higher antioxidant activity and more than double the protection to cells from oxidative damage than traditional collagen hydrogels. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: October 23, 2023

2023 journal article

Scalable and High-Throughput In Vitro Vibratory Platform for Vocal Fold Tissue Engineering Applications

BIOENGINEERING-BASEL, 10(5).

By: A. Biehl n, R. Colmon n, A. Timofeeva n , A. Gracioso Martins n, G. Dion *, K. Peters n , D. Freytes n

author keywords: vocal fold; bioreactor; piezoelectric speaker; vibration; frequency; displacement; fibroblasts; mesenchymal stem cells; gene expression
TL;DR: A scalable and high-throughput platform that mimics the mechanical microenvironment of the VFs in vitro and can incorporate commercial assay formats ranging from 6- to 96-well plates which represents a significant improvement in scalability. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, NC State University Libraries
Added: May 22, 2023

2023 journal article

Tendon Extracellular Matrix Promotes Myotendinous Junction Protein Expression in Engineered Muscle Tissue under Both Static and Mechanically Stimulated Culture Conditions

JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, 2023.

By: L. Gaffney n, M. Fisher n & D. Freytes n

TL;DR: This research combined a relevant microenvironment to study muscle and tendon interactions with a novel bioreactor to apply mechanical strain, an important regulator of the formation and maintenance of the native MTJ. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: September 18, 2023

2022 journal article

Bioreactors for Vocal Fold Tissue Engineering

TISSUE ENGINEERING PART B-REVIEWS, 28(1), 182–205.

By: A. Gracioso Martins n, A. Biehl  n, D. Sze n & D. Freytes n

author keywords: vocal fold; bioreactor; lamina propria; gene expression; fibrosis; inflammation
MeSH headings : Biocompatible Materials; Bioreactors; Cicatrix; Humans; Tissue Engineering / methods; Vocal Cords / pathology; Vocal Cords / physiology
TL;DR: An in-depth analysis of VF bioreactor data to date reveals that mechanical stimulation significantly influences cell viability and the expression of pro-inflammatory and pro-fibrotic genes in vitro. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: March 29, 2021

2022 article

Donor Age and Time in Culture Affect Dermal Fibroblast Contraction in an In Vitro Hydrogel Model

Detwiler, A., Polkoff, K., Gaffney, L., Freytes, D. O., & Piedrahita, J. A. (2022, August 4). TISSUE ENGINEERING PART A.

By: A. Detwiler n, K. Polkoff n, L. Gaffney n, D. Freytes n & J. Piedrahita n

author keywords: cell proliferation; collagen; dermis; extracellular matrix; fibroblasts; hydrogels; skin; wound healing
MeSH headings : Adult; Infant, Newborn; Humans; Swine; Animals; Hydrogels / pharmacology; Reproducibility of Results; Wound Healing; Collagen / chemistry; Fibroblasts
TL;DR: Results show how cellular responses in a hydrogel scaffold differ based on donor age and time in culture in vitro, and suggest that consistency in the cellular component of bioengineered skin products could be beneficial in the biomanufacturing of consistent, reliable skin grafts and graft in vivo models. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 29, 2022

2022 article

High-Axial-Aspect Tannic Acid Microparticles Facilitate Gelation and Injectability of Collagen-Based Hydrogels

Sarker, P., Nalband, D. M., Freytes, D. O., Rojas, O. J., & Khan, S. A. (2022, October 5). BIOMACROMOLECULES, Vol. 10.

By: P. Sarker n, D. Nalband n, D. Freytes n, O. Rojas n & S. Khan n 

MeSH headings : Humans; Hydrogels / chemistry; Tannins; Collagen / chemistry; Elastic Modulus; Rheology
TL;DR: Hydrogels containing TA microparticles show high cell viability and comparative cellular activity relative to the collagen-only hydrogel, demonstrating a new, simple, and better-performance alternative to cell culturing and difficult implantation scenarios. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, NC State University Libraries
Added: October 6, 2022

2022 journal article

Monitoring decellularization via absorbance spectroscopy during the derivation of extracellular matrix scaffolds

BIOMEDICAL MATERIALS, 17(1).

By: C. Mora-Navarro  n, M. Garcia  n, P. Sarker n, E. Ozpinar n , J. Enders n , S. Khan n , R. Branski *, D. Freytes n

author keywords: extracellular matrix; manufacturing; decellularization; monitoring; vocal fold
MeSH headings : Biocompatible Materials; Extracellular Matrix / chemistry; Hydrogels; Regenerative Medicine; Spectrum Analysis; Tissue Engineering / methods; Tissue Scaffolds
TL;DR: Monitoring the bioreactor’s effluent absorbance at 260 nm as a function of time will provide a representative DNA release profile from the tissue and thus allow for process optimization, showing that absorbance profiles are a good representation of DNA removal during the decellularization process thus providing an important tool to optimize future protocols. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, NC State University Libraries
Added: November 4, 2021

2022 article

Towards a standardized multi-tissue decellularization protocol for the derivation of extracellular matrix materials

Biehl, A., Martins, A. M. G. M., Davis, Z. G. G., Sze, D., Collins, L., Mora-Navarro, C., … Freytes, D. O. O. (2022, December 12). BIOMATERIALS SCIENCE, Vol. 12.

By: A. Biehl  n, A. Martins  n, Z. Davis n , D. Sze n, L. Collins n , C. Mora-Navarro *, M. Fisher n, D. Freytes n

MeSH headings : Tissue Engineering / methods; Proteomics; Extracellular Matrix / chemistry; Hydrogels / chemistry; Biocompatible Materials / analysis; Tissue Scaffolds
TL;DR: The present study is the first proof-of-concept towards the development of a standardized protocol that can be used to derive multiple ECM biomaterials via a previously established automated system and showed gelation and rheological properties consistent with previous ECM hydrogels. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: January 3, 2023

2021 journal article

Dermal Extracellular Matrix-Derived Hydrogels as an In Vitro Substrate to Study Mast Cell Maturation

TISSUE ENGINEERING PART A, 27(15-16), 1008–1022.

By: E. Ozpinar n , A. Frey n, G. Arthur n, C. Mora-Navarro  n, A. Biehl  n, D. Snider n, G. Cruse n , D. Freytes n

Contributors: E. Ozpinar n , A. Frey n, G. Arthur n, C. Mora-Navarro  n, A. Biehl  n, D. Snider n, G. Cruse n , D. Freytes n

author keywords: extracellular matrix; mast cell; allergy; metabolism
MeSH headings : Animals; Cell Differentiation; Collagen; Extracellular Matrix; Humans; Hydrogels; Mast Cells; Swine
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID
Added: December 11, 2020

2021 article

Extracellular Matrix Hydrogels Promote Expression of Muscle-Tendon Junction Proteins

Gaffney, L. S., Davis, Z. G., Mora-Navarro, C., Fisher, M. B., & Freytes, D. O. (2021, November 2). TISSUE ENGINEERING PART A, Vol. 11.

By: L. Gaffney n, Z. Davis n , C. Mora-Navarro  n, M. Fisher n & D. Freytes n

author keywords: extracellular matrix; engineered tissues; myotendinous junction; paxillin
MeSH headings : Collagen / metabolism; Collagen Type I / metabolism; Culture Media, Conditioned; Extracellular Matrix / metabolism; Hydrogels; Muscles; Paxillin / metabolism; Tendons / metabolism
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: November 15, 2021

2021 journal article

Mast Cell-Biomaterial Interactions and Tissue Repair

TISSUE ENGINEERING PART B-REVIEWS, 27(6), 590–603.

By: E. Ozpinar n , A. Frey n, G. Cruse n  & D. Freytes n

Contributors: E. Ozpinar n , A. Frey n, G. Cruse n  & D. Freytes n

author keywords: mast cells; biomaterials; inflammation; tissue repair; foreign body response; wound healing
MeSH headings : Biocompatible Materials; Cell Communication; Fibrosis; Humans; Mast Cells / pathology; Tissue Engineering
TL;DR: This review is designed for those in the tissue engineering and biomaterials fields who are interested in exploring the role MCs may play in wound-biomaterial interactions and wound healing and hopes to inspire more research in the MC-Biomaterial space in order to accelerate the design and construction of optimized implants. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID
Added: March 8, 2021

2021 journal article

Microphysiological System for High-Throughput Computer Vision Measurement of Microtissue Contraction

ACS SENSORS, 6(3), 985–994.

By: A. Martins  n, M. Wilkins  n, F. Ligler n, M. Daniele n & D. Freytes n

author keywords: high throughput; 3D culture; tissue function; tissue contraction; computer vision
MeSH headings : Computers; Fibroblasts; High-Throughput Screening Assays; Humans; Image Processing, Computer-Assisted
TL;DR: An inexpensive, versatile, high-throughput platform to measure microtissue contraction in a 96-well plate configuration using one-step batch imaging and an algorithm uses pixel-based image analysis and computer vision techniques for the accurate multiwell quantification of positional changes in the optical microprobes caused by the contraction of the microtissues. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: May 10, 2021

2021 journal article

Transcriptome-targeted analysis of human peripheral blood-derived macrophages when cultured on biomaterial meshes

BIOMEDICAL MATERIALS, 16(2).

By: C. Mora-Navarro  n, E. Ozpinar n , D. Sze n, D. Martin * & D. Freytes n

author keywords: surgical meshes; macrophage polarization; biomaterials; targeted transcriptome; poly-4-hydroxybutyrate (P4HB); polypropylene
MeSH headings : Anti-Inflammatory Agents / chemistry; Biocompatible Materials / chemistry; Cell Culture Techniques; Cell Differentiation; Cell Separation; Cells, Cultured; Flow Cytometry; Gene Expression Profiling; Humans; Hydroxybutyrates; Immunity, Innate; In Vitro Techniques; Inflammation; Macrophages / drug effects; Macrophages / metabolism; Materials Testing; Monocytes / cytology; Phenotype; Polypropylenes / chemistry; Prostheses and Implants; RNA / metabolism; Surgical Mesh; Tissue Scaffolds; Transcriptome; Treatment Outcome; Up-Regulation; Wound Healing
TL;DR: In vitro evidence is provided for the early transcriptomic targeted signature of human Mφs upon two commonly used surgical meshes and suggests a transition from an inflammatory to a non-inflammatory phenotype by P4HB as well as an upregulation of genes annotated under the pathogen response pathway. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: January 16, 2021

2020 journal article

Photoinduced reconfiguration to control the protein-binding affinity of azobenzene-cyclized peptides

JOURNAL OF MATERIALS CHEMISTRY B, 8(33), 7413–7427.

By: K. Day n, J. Schneible n, A. Young n, V. Pozdin*, G. Driessche  n, L. Gaffney n, R. Prodromou n, D. Freytes n ...

MeSH headings : Amino Acid Sequence; Azo Compounds / chemistry; Hydrogen-Ion Concentration; Isomerism; Osmolar Concentration; Peptides, Cyclic / chemistry; Photochemical Processes
TL;DR: This study demonstrates a general method to develop azobenzene-cyclized peptide ligands with light-controlled affinity for target proteins by designing photo-switchable peptides targeting vascular cell adhesion marker 1 (VCAM1), a cell marker implicated in stem cell function. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: September 14, 2020

2020 journal article

Platelet-like particles improve fibrin network properties in a hemophilic model of provisional matrix structural defects

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 577, 406–418.

By: S. Nandi n, L. Sommerville *, K. Nellenbach n, E. Mihalko n, M. Erb n, D. Freytes n, M. Hoffman *, D. Monroe *, A. Brown n

author keywords: Fibrin; Biomimetic; Platelet; Hemophilia; Biomaterials
MeSH headings : Blood Coagulation; Blood Platelets; Fibrin; Plasma; Wound Healing
TL;DR: PLPs improved clot network density, force generation, and stiffness, and promoted fibroblast migration within an in vitro model of early wound healing under hemophilic conditions, indicating that PLPs could provide a biomimetic platform for improving wound healing events in disease conditions that cause deficient fibrin network formation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 24, 2020

2019 journal article

Platelet-like particles dynamically stiffen fibrin matrices and improve wound healing outcomes

BIOMATERIALS SCIENCE, 7(2), 669–682.

By: S. Nandi n, E. Sproul n, K. Nellenbach n, M. Erb n, L. Gaffney n, D. Freytes n, A. Brown n

MeSH headings : Animals; Biomimetic Materials / chemistry; Blood Platelets / metabolism; Blood Platelets / physiology; Fibrin / chemistry; Fibrin / pharmacology; Humans; Mechanical Phenomena; Mice; Platelet Activation / drug effects; Skin / drug effects; Skin / injuries; Wound Healing / drug effects
TL;DR: The utility of PLPs for enhancing wound repair is demonstrated and the role of native platelet-mediated clot retraction in wound healing is provided, to provide insight into the role in vitro and in vivo models of wound healing. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: February 18, 2019

2018 journal article

Enhanced tissue remodelling efficacy of adipose-derived mesenchymal stem cells using injectable matrices in radiation-damaged salivary gland model

JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, 12(2), E695–E706.

By: J. Choi, H. An, H. Shin, Y. Kim & J. Lim

author keywords: salivary gland; adipose tissue-derived mesenchymal stem cells; small intestinal submucosa gel; radiation; regeneration; local transplantation
MeSH headings : Bone Morphogenetic Proteins / pharmacology; Embryonic Stem Cells / cytology; Embryonic Stem Cells / drug effects; Gene Expression Regulation / drug effects; Heart, Artificial; Human Umbilical Vein Endothelial Cells / metabolism; Humans; Imaging, Three-Dimensional; Models, Biological; Myocardium / pathology; Neovascularization, Physiologic / drug effects; Wound Healing / drug effects
TL;DR: A model is proposed of cardiac tissues preconditioned with B MPs that results in stimulation of cardiomyocyte function and diffusion of BMPs able to support angiogenesis and represents a step towards the validation of more complex bioengineered constructs for in vivo applications. (via Semantic Scholar)
UN Sustainable Development Goal Categories
1. No Poverty (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2018 journal article

Mesenchymal Stem Cell/Red Blood Cell-Inspired Nanoparticle Therapy in Mice with Carbon Tetrachloride-Induced Acute Liver Failure

ACS Nano, 12(7), 6536–6544.

By: H. Liang n, K. Huang n , T. Su n, Z. Li  n, S. Hu  n, P. Dinh  n, E. Wrona n, C. Shao * ...

Contributors: H. Liang n, K. Huang n , T. Su n, Z. Li  n, S. Hu  n, P. Dinh  n, E. Wrona n, C. Shao * ...

author keywords: mesenchymal stem cells; red blood cells; biomimetic; nanoparticles; membrane cloaking; liver failure
MeSH headings : Animals; Apoptosis; Biomimetic Materials / chemistry; Biomimetic Materials / therapeutic use; Carbon Tetrachloride; Cell Line; Cell Proliferation; Disease Models, Animal; Erythrocyte Membrane / chemistry; Humans; Liver Failure, Acute / chemically induced; Liver Failure, Acute / pathology; Liver Failure, Acute / physiopathology; Liver Failure, Acute / therapy; Liver Regeneration; Male; Mesenchymal Stem Cells / chemistry; Mice; Mice, Inbred C57BL; Nanoparticles / chemistry; Nanoparticles / therapeutic use
TL;DR: This work fabricated a nanoparticle that carries the beneficial regenerative factors from mesenchymal stem cells and further coated it with the membranes of red blood cells to increase blood stability and promote liver cell proliferation in vitro and have lower internalization by macrophage cells. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Crossref, ORCID, NC State University Libraries
Added: September 10, 2019

2018 review

Potential Synergistic Effects of Stem Cells and Extracellular Matrix Scaffolds

[Review of ]. ACS BIOMATERIALS SCIENCE & ENGINEERING, 4(4), 1208–1222.

By: L. Gaffney n, E. Wrona n & D. Freytes n

Contributors: L. Gaffney n, E. Wrona n & D. Freytes n

author keywords: extracellular matrix (ECM); stem cells; pluripotent stem cells (PSCs); wound healing; macrophage polarization
TL;DR: This work has shown that extracellular matrix-derived biomaterials, used as scaffolds to help regenerate diseased or damaged tissues, provide a scaffold material with native-like composition and structure through several mechanisms that include chemotactic properties, growth factor release, and modulation of the immune response. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2017 journal article

BMP protein-mediated crosstalk between inflammatory cells and human pluripotent stem cell-derived cardiomyocytes

Journal of Tissue Engineering and Regenerative Medicine, 11(5), 1466–1478.

By: I. Pallotta*, B. Sun*, E. Wrona* & D. Freytes*

Contributors: I. Pallotta*, B. Sun*, E. Wrona* & D. Freytes*

author keywords: cardiomyocytes; macrophages; inflammation; BMP; migration; tissue engineering; regenerative medicine
MeSH headings : Bone Morphogenetic Protein 4 / metabolism; Cell Communication; Humans; Inflammation / metabolism; Inflammation / pathology; Macrophages / metabolism; Macrophages / pathology; Myocytes, Cardiac / metabolism; Myocytes, Cardiac / pathology; Pluripotent Stem Cells / metabolism; Pluripotent Stem Cells / pathology; Smad Proteins / metabolism
TL;DR: A new role is proposed for CM‐ and macrophage‐derived BMP proteins during the recruitment ofmacrophage subtypes and the maturation of repair cells, representing an important step towards creating a functional cardiac patch with superior therapeutic properties. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Crossref, ORCID
Added: September 10, 2019

2017 journal article

Directed Differentiation of Human Pluripotent Stem Cells to Microglia

STEM CELL REPORTS, 8(6), 1516–1524.

By: P. Douvaras *, B. Sun*, M. Wang *, I. Kruglikov*, G. Lallos*, M. Zimmer*, C. Terrenoire*, B. Zhang * ...

MeSH headings : Adenosine Diphosphate / pharmacology; CX3C Chemokine Receptor 1 / metabolism; Calcium / metabolism; Cell Differentiation; Cell Line; Cytokines / metabolism; Gene Expression; Human Embryonic Stem Cells / cytology; Human Embryonic Stem Cells / metabolism; Humans; Lipopolysaccharide Receptors / metabolism; Macrophages / cytology; Macrophages / metabolism; Microglia / cytology; Microglia / drug effects; Microglia / metabolism; Pluripotent Stem Cells / cytology; Pluripotent Stem Cells / metabolism
TL;DR: iPSC-MG were able to phagocytose and responded to ADP by producing intracellular Ca2+ transients, whereas macrophages lacked such response, and the differentiation protocol was highly reproducible across several pluripotent stem cell lines. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2017 journal article

Macrophages' role in tissue disease and regeneration

Macrophages: Origin, Functions and Biointervention, 62, 245–271.

By: L. Gaffney, P. Warren, E. Wrona, M. Fisher & D. Freytes

Source: NC State University Libraries
Added: August 6, 2018

2016 journal article

Differential gene expression in human, murine, and cell line-derived macrophages upon polarization

Experimental Cell Research, 347(1), 1–13.

By: K. Spiller *, E. Wrona*, S. Romero-Torres, I. Pallotta*, P. Graney *, C. Witherel*, L. Panicker*, R. Feldman * ...

Contributors: K. Spiller *, E. Wrona*, S. Romero-Torres, I. Pallotta*, P. Graney *, C. Witherel*, L. Panicker*, R. Feldman * ...

author keywords: Classically activated; Alternatively activated macrophages; Wound healing; In vitro characterization; Angiogenesis; Tissue engineering; Induced pluripotent stem cells; Biomaterials
MeSH headings : Animals; Biocompatible Materials / pharmacology; Cell Differentiation / drug effects; Cell Line; Cell Polarity / drug effects; Cell Polarity / genetics; Discriminant Analysis; Gene Expression Profiling; Gene Expression Regulation / drug effects; Humans; Interferon-gamma / pharmacology; Least-Squares Analysis; Lipopolysaccharides / pharmacology; Macrophages / cytology; Macrophages / drug effects; Macrophages / metabolism; Mice, Inbred BALB C; Phenotype
TL;DR: Multivariate discriminant analysis was used to compare the in vitro expression of genes commonly chosen to assess macrophage phenotype across all three sources of macrophages, as well as those derived from induced pluripotent stem cells (iPSCs), that were polarized towards four distinct phenotypes using the same differentiation protocols. (via Semantic Scholar)
UN Sustainable Development Goal Categories
10. Reduced Inequalities (OpenAlex)
Sources: Crossref, ORCID
Added: September 10, 2019

2016 review

Extracellular Matrix for Vocal Fold Lamina Propria Replacement: A Review

[Review of ]. TISSUE ENGINEERING PART B-REVIEWS, 22(6), 421–429.

By: E. Wrona n, R. Peng*, M. Amin*, R. Branski * & D. Freytes n

Contributors: E. Wrona n, R. Peng*, M. Amin*, R. Branski * & D. Freytes n

author keywords: voice; vocal fold; extracellular matrix; scaffold
MeSH headings : Extracellular Matrix; Humans; Mucous Membrane; Regenerative Medicine; Tissue Engineering; Vocal Cords
TL;DR: This review explores the concept of tissue engineering as it relates to the VFs, as well as recent studies employing both naturally and synthetically derived biomaterials in combination with stem cells for a tissue-engineered approach to VF repair. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2016 journal article

Response of human macrophages to wound matrices in vitro

WOUND REPAIR AND REGENERATION, 24(3), 514–524.

By: C. Witherel*, P. Graney *, D. Freytes n, M. Weingarten * & K. Spiller *

MeSH headings : Acellular Dermis; Biocompatible Materials / pharmacology; Biological Dressings; Cell Culture Techniques; Cell Differentiation; Cells, Cultured; Cellular Microenvironment / drug effects; Cellular Microenvironment / physiology; Humans; Macrophages / drug effects; Macrophages / metabolism; Materials Testing; Neovascularization, Physiologic / drug effects; Neovascularization, Physiologic / physiology; Phenotype; Tissue Scaffolds; Wound Healing / drug effects; Wound Healing / physiology
TL;DR: It was found that collagen scaffolds cross‐linked with 1‐ethyl‐3‐(3‐dimethylaminopropyl) carbodiimide and N‐hydroxysuccinimide (EDC/NHS) promoted the least inflammatory phenotype in primary human macrophages compared with scaffoldsCross‐ linked with formaldehyde or glutaraldehyde. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2015 journal article

Controlled release of cytokines using silk-biomaterials for macrophage polarization

Biomaterials, 73, 272–283.

By: A. Reeves*, K. Spiller *, D. Freytes*, G. Vunjak-Novakovic * & D. Kaplan *

author keywords: Macrophage; Polarization; Silk; Cytokine
MeSH headings : Aniline Compounds / chemistry; Animals; Biocompatible Materials / chemistry; Cytokines / metabolism; Delayed-Action Preparations / metabolism; Humans; Interferon-gamma / administration & dosage; Interleukin-4 / administration & dosage; Macrophages / cytology; Macrophages / metabolism; Mice; Microscopy, Electron, Scanning; Phenotype; Protein Structure, Secondary; Real-Time Polymerase Chain Reaction; Silk / chemistry; Solubility; Time Factors; Water / chemistry
TL;DR: The silk biomaterials were able to release enough IFN-γ or IL-4 to repolarize the macrophage from M1 to M2 and vice versa, demonstrating the well-established plasticity of macrophages. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Crossref
Added: February 24, 2020

2015 journal article

Derivation and characterization of porcine vocal fold extracellular matrix scaffold

The Laryngoscope, 126(4), 928–935.

By: E. Wrona*, R. Peng*, H. Born *, M. Amin*, R. Branski * & D. Freytes*

Contributors: E. Wrona*, R. Peng*, H. Born *, M. Amin*, R. Branski * & D. Freytes*

author keywords: Vocal fold; extracellular matrix; mesenchymal stem cells; scaffold
MeSH headings : Animals; Cells, Cultured; DNA / analysis; Deoxycholic Acid; Extracellular Matrix / physiology; Gene Expression; Mesenchymal Stem Cells / cytology; Peracetic Acid; Swine; Tissue Engineering / methods; Tissue Scaffolds; Vocal Cords / cytology
TL;DR: To optimize decellularization of porcine vocal folds (VF) and quantify human bone marrow‐derived mesenchymal stem cell interactions with this matrix to provide a foundation for regenerative approaches to VF repair. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: September 10, 2019

2015 journal article

Reprint of: Extracellular matrix as a biological scaffold material: Structure and function

Acta Biomaterialia, 23, S17–S26.

By: S. Badylak *, D. Freytes* & T. Gilbert*

TL;DR: An overview of the composition and structure of selected ECM scaffolding materials, the effects of manufacturing methods upon the structural properties and resulting mechanical behavior of the scaffold materials, and the in vivo degradation and remodeling of ECm scaffolds with an emphasis on tissue function is provided. (via Semantic Scholar)
Source: Crossref
Added: February 24, 2020

2014 journal article

Macrophages Modulate Engineered Human Tissues for Enhanced Vascularization and Healing

Annals of Biomedical Engineering, 43(3), 616–627.

By: K. Spiller *, D. Freytes* & G. Vunjak-Novakovic *

author keywords: Scaffold; Vascularization; Inflammatory response; Tissue engineering; Healing
MeSH headings : Animals; Humans; Inflammation / immunology; Macrophages / physiology; Neovascularization, Physiologic; Regeneration; Tissue Engineering; Tissue Scaffolds
TL;DR: This work discusses here the cell–scaffold–bioreactor systems for harnessing the inflammatory response for enhanced tissue vascularization and healing and a new generation of “smart” tissue engineering systems designed to actively mediate tissue survival and function. (via Semantic Scholar)
Source: Crossref
Added: January 21, 2021

2013 journal article

Decellularization of Human and Porcine Lung Tissues for Pulmonary Tissue Engineering

The Annals of Thoracic Surgery, 96(3), 1046–1056.

By: J. O'Neill *, R. Anfang*, A. Anandappa*, J. Costa *, J. Javidfar *, H. Wobma *, G. Singh *, D. Freytes* ...

MeSH headings : Animals; Bioartificial Organs; Biomechanical Phenomena; Cell Separation / methods; Elastin / physiology; Extracellular Matrix / physiology; Feasibility Studies; Humans; Immunohistochemistry; Lung / cytology; Lung Transplantation / methods; Materials Testing; Microscopy, Electron, Scanning; Sensitivity and Specificity; Swine; Tensile Strength; Tissue Engineering / methods; Tissue Scaffolds
TL;DR: Porcine lungs can be decellularized with CHAPS to produce LECM scaffolds with properties resembling those of human lungs, for pulmonary tissue engineering. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2013 journal article

The regulation of growth and metabolism of kidney stem cells with regional specificity using extracellular matrix derived from kidney

Biomaterials, 34(38), 9830–9841.

By: J. O'Neill *, D. Freytes*, A. Anandappa*, J. Oliver* & G. Vunjak-Novakovic *

author keywords: Extracellular matrix; Hydrogels; Kidney; Stem cells; Niche; Regional specificity
MeSH headings : Animals; Cells, Cultured; Extracellular Matrix / metabolism; Extracellular Matrix / ultrastructure; Immunohistochemistry; Kidney / cytology; Mesenchymal Stem Cells / cytology; Mesenchymal Stem Cells / metabolism; Mesenchymal Stem Cells / ultrastructure; Mice; Microscopy, Electron, Scanning; Stem Cells / cytology; Stem Cells / metabolism; Stem Cells / ultrastructure; Swine
TL;DR: The data suggest that tissue- and region-specific ECM can provide an effective substrate for in vitro studies of therapeutic stem cells, and that specialized bioengineered niches for stem cells can be established using ECM-derived scaffolding materials. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2012 journal article

Macrophages modulate the viability and growth of human mesenchymal stem cells

Journal of Cellular Biochemistry, 114(1), 220–229.

By: D. Freytes*, J. Kang, I. Marcos-Campos & G. Vunjak-Novakovic

author keywords: MESENCHYMAL STEM CELLS; MACROPHAGES; INFLAMMATION
MeSH headings : Cell Communication; Cell Line; Cell Proliferation / drug effects; Cell Survival / drug effects; Coculture Techniques; Cytokines / pharmacology; Humans; Inflammation / metabolism; Inflammation / pathology; Lipopolysaccharides / pharmacology; Macrophages / cytology; Macrophages / drug effects; Macrophages / metabolism; Mesenchymal Stem Cells / cytology; Mesenchymal Stem Cells / drug effects; Mesenchymal Stem Cells / metabolism; Models, Biological; Vascular Endothelial Growth Factor A / pharmacology
TL;DR: Data imply that an anti‐inflammatory (M2) environment is more accommodating to the therapeutic hMSCs than a pro‐ inflammatory (M1) environment at specific concentrations. (via Semantic Scholar)
Source: Crossref
Added: January 21, 2021

2011 journal article

549 Perfusion/Decellularization of Large Animal Lungs

The Journal of Heart and Lung Transplantation, 30(4), S184–S185.

By: G. Singh *, J. Javidfar *, J. Costa *, J. Guarrera *, J. Miller*, S. Henry*, Q. Jallerat*, D. Freytes* ...

UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: Crossref
Added: August 28, 2020

2011 journal article

Hybrid Gel Composed of Native Heart Matrix and Collagen Induces Cardiac Differentiation of Human Embryonic Stem Cells without Supplemental Growth Factors

Journal of Cardiovascular Translational Research, 4(5), 605–615.

By: Y. Duan *, Z. Liu*, J. O’Neill *, L. Wan *, D. Freytes* & G. Vunjak-Novakovic *

author keywords: Tissue engineering; Cardiac differentiation; Human embryonic stem cells; Biomaterial; Native cardiac matrix
MeSH headings : Animals; Biomarkers / metabolism; Cell Differentiation; Cells, Cultured; Coculture Techniques; Collagen Type I / metabolism; Connexin 43 / metabolism; Embryonic Stem Cells / metabolism; Embryonic Stem Cells / transplantation; Extracellular Matrix / metabolism; Humans; Hydrogels; Myocardial Contraction; Myocytes, Cardiac / metabolism; Myocytes, Cardiac / transplantation; Regenerative Medicine / methods; Swine; Time Factors; Tissue Engineering; Tissue Scaffolds; Troponin I / metabolism; Troponin T / genetics; Up-Regulation
TL;DR: The ability of native ECM hydrogel to induce cardiac differentiation of hESCs without the addition of soluble factors makes it an attractive biomaterial system for basic studies of cardiac development and potentially for the delivery of therapeutic cells into the heart. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2009 journal article

Extracellular matrix as a biological scaffold material: Structure and function

Acta Biomaterialia, 5(1), 1–13.

By: S. Badylak *, D. Freytes* & T. Gilbert*

author keywords: Extracellular matrix; Scaffold; Remodeling; Degradation; Ultrastructure
MeSH headings : Animals; Biochemistry / methods; Biocompatible Materials / chemistry; Biomedical Engineering; Collagen / chemistry; Extracellular Matrix / chemistry; Extracellular Matrix / physiology; Freeze Drying; Humans; Materials Testing; Microscopy, Electron, Scanning; Models, Biological; Stress, Mechanical; Tissue Engineering / methods; Tissue Scaffolds
TL;DR: An overview of the composition and structure of selected ECM scaffolding materials, the effects of manufacturing methods upon the structural properties and resulting mechanical behavior of the scaffold materials, and the in vivo degradation and remodeling of ECm scaffolds with an emphasis on tissue function is provided. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2009 journal article

Geometry and force control of cell function

Journal of Cellular Biochemistry, 108(5), 1047–1058.

By: D. Freytes*, L. Wan * & G. Vunjak-Novakovic *

author keywords: STEM CELLS; BIOPHYSICAL REGULATION; SCAFFOLDS; BIOREACTORS; TISSUE ENGINEERING
MeSH headings : Animals; Biomedical Research; Biomimetics / methods; Cell Culture Techniques; Humans; Models, Biological; Rats; Regeneration; Stem Cells / cytology; Stem Cells / physiology; Stress, Physiological; Tissue Engineering / methods; Tissue Scaffolds; Transplants
TL;DR: Tissue engineering is becoming increasingly ambitious in its efforts to create functional human tissues, and to provide stem cell scientists with culture systems of high biological fidelity, with emphasis on the derivation of design principles from the development and the geometry‐force control of cell function. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2009 journal article

Oxygen diffusivity of biologic and synthetic scaffold materials for tissue engineering

Journal of Biomedical Materials Research Part A, 91A(4), 1010–1017.

By: J. Valentin*, D. Freytes*, J. Grasman *, C. Pesyna*, J. Freund*, T. Gilbert*, S. Badylak *

author keywords: biologic scaffold material; extracellular matrix; tissue engineering; oxygen diffusivity; ultrastructure
MeSH headings : Animals; Biocompatible Materials / pharmacology; Diffusion / drug effects; Extracellular Matrix / drug effects; Extracellular Matrix / ultrastructure; Materials Testing; Oxygen / metabolism; Sus scrofa; Temperature; Time Factors; Tissue Engineering / methods; Tissue Scaffolds / chemistry
TL;DR: Each material composed of ECM has unique oxygen diffusivity values, and these values may be dependent on the scaffold's ultrastructure, the current study showed. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2008 journal article

Collagen fiber alignment and biaxial mechanical behavior of porcine urinary bladder derived extracellular matrix

Biomaterials, 29(36), 4775–4782.

By: T. Gilbert*, S. Wognum*, E. Joyce*, D. Freytes*, M. Sacks * & S. Badylak *

author keywords: Extracellular matrix; Scaffold; Collagen fiber alignment; Biaxial mechanical behavior
MeSH headings : Animals; Biomechanical Phenomena; Elastic Modulus; Extracellular Matrix / metabolism; Fibrillar Collagens / metabolism; Mucous Membrane / metabolism; Sus scrofa / metabolism; Urinary Bladder / metabolism
TL;DR: Evaluating the collagen fiber alignment and biaxial mechanical behavior of ECM scaffold material harvested from porcine urinary bladder tunica mucosa and basement membrane found UBML and UBS showed quite different mechanical behavior for the applied loading patterns with UBS showing a much more pronounced toe region. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2008 journal article

Hydrated versus lyophilized forms of porcine extracellular matrix derived from the urinary bladder

Journal of Biomedical Materials Research Part A, 87A(4), 862–872.

By: D. Freytes*, R. Tullius*, J. Valentin*, A. Stewart-Akers* & S. Badylak *

author keywords: extracellular matrix; lyophilization; freeze-drying; scaffold; structural properties; urinary bladder
MeSH headings : Animals; Biocompatible Materials / chemistry; Extracellular Matrix / chemistry; Freeze Drying; Humans; Hydrostatic Pressure; Materials Testing; Mice; NIH 3T3 Cells; Permeability; Stress, Mechanical; Swine; Tissue Engineering / methods; Tissue Scaffolds / chemistry; Urinary Bladder / anatomy & histology; Urinary Bladder / chemistry; Water / chemistry
TL;DR: It is concluded that lyophilization affects the overall in vitro cell growth of NIH 3T3 cells and the ultrastructural morphology of UBM devices, but does not result in significant changes in structural properties. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Crossref
Added: August 28, 2020

2008 journal article

Preparation and rheological characterization of a gel form of the porcine urinary bladder matrix

Biomaterials, 29(11), 1630–1637.

By: D. Freytes*, J. Martin*, S. Velankar *, A. Lee * & S. Badylak *

author keywords: extracellular matrix; viscoelasticity; rheology; scaffold; gel
MeSH headings : Animals; Cell Adhesion; Cell Proliferation; Cells, Cultured; Extracellular Matrix / metabolism; Extracellular Matrix / ultrastructure; Gels / chemistry; Gels / metabolism; Kinetics; Microscopy, Electron, Scanning; Myocytes, Smooth Muscle / cytology; Rats; Rheology; Swine; Tissue Scaffolds / chemistry; Urinary Bladder / metabolism; Viscosity
TL;DR: An intact form of UBM can be successfully solubilized without purification steps and induced to repolymerize into a gel form of the UBM biologic scaffold material, which supported the adhesion and growth of rat aortic smooth muscle cells when cultured under static in vitro conditions. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2008 journal article

Uniaxial and biaxial properties of terminally sterilized porcine urinary bladder matrix scaffolds

Journal of Biomedical Materials Research Part B: Applied Biomaterials, 84B(2), 408–414.

By: D. Freytes*, R. Stoner* & S. Badylak *

author keywords: sterilization; extracellular matrix; material properties; strength; scaffolds
MeSH headings : Animals; Biocompatible Materials; Extracellular Matrix; Materials Testing; Sterilization; Swine; Tensile Strength; Tissue Scaffolds; Urinary Bladder
TL;DR: The present study showed that the choice of terminal sterilization method affects the structural properties of scaffolds composed of extracellular matrix. (via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Source: Crossref
Added: August 28, 2020

2006 journal article

Effect of storage upon material properties of lyophilized porcine extracellular matrix derived from the urinary bladder

Journal of Biomedical Materials Research Part B: Applied Biomaterials, 78B(2), 327–333.

By: D. Freytes*, R. Tullius* & S. Badylak *

author keywords: stability; suture; scaffold; permeability; mechanical properties; urinary bladder; extracellular matrix
MeSH headings : Animals; Bioprosthesis; Extracellular Matrix; Freeze Drying; Materials Testing; Swine; Time Factors; Urinary Bladder
TL;DR: Examination of material properties for lyophilized forms of an ECM scaffold derived from the porcine urinary bladder shows that structural changes in the UBM device may slowly occur as a function of prolonged storage and storage temperature. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2006 journal article

Fiber architecture and biaxial mechanical behavior of porcine urinary bladder extracellular matrix

Journal of Biomechanics, 39, S391.

By: T. Gilbert, D. Freytes*, A. Willment, M. Sacks & S. Badylak

Source: Crossref
Added: August 28, 2020

2005 journal article

Analytically derived material properties of multilaminated extracellular matrix devices using the ball-burst test

Biomaterials, 26(27), 5518–5531.

By: D. Freytes*, A. Rundell*, J. Vandegeest*, D. Vorp *, T. Webster* & S. Badylak *

author keywords: bladder; ECM; multilaminate devices; mechanical properties; mechanical test; ball-burst test; scaffold
MeSH headings : Animals; Biocompatible Materials / analysis; Biocompatible Materials / chemistry; Biomechanical Phenomena / methods; Compressive Strength; Computer Simulation; Elasticity; Extracellular Matrix / chemistry; Extracellular Matrix / physiology; Manufactured Materials / analysis; Materials Testing / methods; Models, Biological; Models, Chemical; Physical Stimulation / methods; Swine; Tensile Strength
TL;DR: The ball-burst test was used to simulate biaxial forces and to determine the strength of the ECM scaffold under a relevant physiological loading condition and proposed approximations were used to compute biaXial failure properties for a variety of multilaminate ECM devices with varying number of layers. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2005 journal article

Esophageal Reconstruction with ECM and Muscle Tissue in a Dog Model

Journal of Surgical Research, 128(1), 87–97.

By: S. Badylak *, D. Vorp *, A. Spievack*, A. Simmons-Byrd*, J. Hanke*, D. Freytes*, A. Thapa*, T. Gilbert*, A. Nieponice*

author keywords: extracellular matrix; urinary bladder matrix; esophagus; surgery; tissue engineering; scaffold; esophageal cancer; regenerative medicine; bioscaffold
MeSH headings : Animals; Biomechanical Phenomena; Dogs; Esophageal Stenosis / etiology; Esophageal Stenosis / prevention & control; Esophagectomy / adverse effects; Esophagus / physiopathology; Esophagus / surgery; Extracellular Matrix / physiology; Female; Models, Animal; Muscle, Smooth / physiology; Muscle, Smooth / surgery; Plastic Surgery Procedures / methods; Recovery of Function; Tissue Engineering / methods
TL;DR: It is concluded that ECM bioscaffolds plus autologous muscle tissue, but not ECM scaffolds or muscle tissue alone, can facilitate the in situ reconstitution of structurally and functionally acceptable esophageal tissue. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2004 journal article

Biaxial strength of multilaminated extracellular matrix scaffolds

Biomaterials, 25(12), 2353–2361.

By: D. Freytes*, S. Badylak *, T. Webster*, L. Geddes* & A. Rundell*

author keywords: ECM (extracellular matrix); ball-burst strength; mechanical properties; SEM (scanning electron microscopy); SIS (small intestinal submucosa); disinfection
MeSH headings : Absorbable Implants; Animals; Biocompatible Materials; Biological Factors / physiology; Cells, Cultured; Dogs; Extracellular Matrix / physiology; Extracellular Matrix / ultrastructure; Gastric Mucosa / physiology; Gastric Mucosa / ultrastructure; Humans; Jejunum / physiology; Jejunum / ultrastructure; Manufactured Materials / analysis; Materials Testing / methods; Organ Specificity; Swine; Tensile Strength / physiology; Urinary Bladder / physiology; Urinary Bladder / ultrastructure
TL;DR: The material properties of the ECM scaffolds could be engineered to mimic those of native soft tissues (i.e. vascular, musculotendinous, etc) by varying the number of layers and modifying the disinfection/sterilization treatments used for manufacturing. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

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