Molecular Education, Technology and Research Innovation Center

Works Published in 2007

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Displaying works 21 - 31 of 31 in total

Sorted by most recent date added to the index first, which may not be the same as publication date order.

2007 journal article

Differential protein expression in male and female human lumbar cerebrospinal fluid using iTRAQ reagents after abundant protein depletion

PROTEOMICS, 7(20), 3726–3734.

By: Y. Ogata*, M. Charlesworth*, L. Higgins*, B. Keegan*, S. Vernino* & D. Muddiman n

author keywords: ceruloplasmin; ferroxidase; gender; low-abundance protein; mass spectrometry
MeSH headings : Adult; Aged; Biomarkers / analysis; Biomarkers / metabolism; Cerebrospinal Fluid Proteins / analysis; Cerebrospinal Fluid Proteins / metabolism; Female; Humans; Indicators and Reagents; Lumbosacral Region; Male; Middle Aged; Proteome / analysis; Proteome / metabolism
TL;DR: This study examined the differential expression of proteins in pooled male and female CSF utilizing isobaric tags for relative and absolute quantification (iTRAQ™) reagents after the depletion of six high abundant proteins using a multiple affinity removal system (MARS). (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 journal article

Achieving augmented limits of detection for peptides with hydrophobic alkyl tags

ANALYTICAL CHEMISTRY, 79(11), 3989–3995.

MeSH headings : Acetates / chemistry; Alkylation; Amides / chemical synthesis; Amides / chemistry; Amino Acid Sequence; Chlorides / chemistry; Hydrophobic and Hydrophilic Interactions; Iodine / chemistry; Molecular Sequence Data; Peptides / analysis; Peptides / chemistry; Sensitivity and Specificity; Spectrometry, Mass, Electrospray Ionization; Tandem Mass Spectrometry
TL;DR: The results demonstrate the feasibility of the octylcarboxyamidomethyl modification to improve limits of detection for cysteine-containing peptides with hydrophobic alkyl tags. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 journal article

Parts-per-billion mass measurement accuracy achieved through the combination of multiple linear regression and automatic gain control in a Fourier transform ion cyclotron resonance mass spectrometer

ANALYTICAL CHEMISTRY, 79(13), 5058–5063.

By: D. Williams n & D. Muddiman n

MeSH headings : Automation; Calibration; Cyclotrons / instrumentation; Ions; Mass Spectrometry / instrumentation; Mass Spectrometry / methods; Regression Analysis; Reproducibility of Results; Sensitivity and Specificity
TL;DR: Calibrated multiple linear regression calibration is used to account for both differences in total ion population in the ICR cell as well as relative ion abundance of a given species, which affords mean MMA values at the parts-per-billion (ppb) level. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 review

Epithelial ovarian cancer: Disease etiology, treatment, detection, and investigational gene, metabolite, and protein biomarkers

[Review of ]. JOURNAL OF PROTEOME RESEARCH, 6(8), 2936–2962.

By: T. Williams n, K. Toups n, D. Saggese n, K. Kalli n, W. Cliby n & D. Muddiman n

Contributors: T. Williams n, K. Toups n, D. Saggese n, K. Kalli n, W. Cliby n & D. Muddiman n

author keywords: biomarkers; epithelial ovarian cancer; proteomics; mass spectrometry; detection; treatments
MeSH headings : Biomarkers, Tumor / metabolism; Female; Humans; Neoplasms, Glandular and Epithelial / diagnosis; Neoplasms, Glandular and Epithelial / etiology; Neoplasms, Glandular and Epithelial / metabolism; Ovarian Neoplasms / diagnosis; Ovarian Neoplasms / etiology; Ovarian Neoplasms / metabolism
TL;DR: An introduction to ovarian cancer is offered, with particular emphasis on human epithelial ovarian cancer, and a survey of promising new protein, gene, and metabolite biomarkers on the horizon is provided. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2007 journal article

Detection of attomole amounts of analyte by desorption electrospray ionization mass spectrometry (DESI-MS) determined using fluorescence spectroscopy

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 18(6), 1093–1096.

By: M. Bereman n & D. Muddiman n

MeSH headings : Microchemistry / methods; Nanotechnology / methods; Rhodamines / chemistry; Spectrometry, Fluorescence / methods; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR: The use of fluorescence spectroscopy is reported to investigate the amount of material removed from a PTFE surface and detected during desorption electrospray ionization (DESI) mass spectrometry measurements. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 journal article

Understanding the influence of post-excite radius and axial confinement on quantitative proteomic measurements using Fourier transform ion cyclotron resonance mass spectrometry

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 21(7), 1196–1204.

By: J. Frahm n, C. Capo Velez* & D. Muddiman n

MeSH headings : Artifacts; Cyclotrons; Ions; Peptide Mapping / methods; Proteome / analysis; Proteome / chemistry; Reproducibility of Results; Sensitivity and Specificity; Sequence Analysis, Protein / methods; Spectrometry, Mass, Electrospray Ionization / methods; Spectroscopy, Fourier Transform Infrared / methods
TL;DR: To adequately assess the multiple parameters in the large dataset, a multiplicative quality factor is developed that encompasses the total ion abundance, as well as the accuracy and the precision of abundance ratios, which allows facile determination of optimal instrument parameters for quantitative measurements. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 journal article

Direct characterization of intact polypeptides by matrix-assisted laser desorption electrospray ionization quadrupole Fourier transform ion cyclotron resonance mass spectrometry

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 21(7), 1150–1154.

MeSH headings : Cyclotrons; Microchemistry / methods; Molecular Weight; Peptides / chemistry; Reproducibility of Results; Sensitivity and Specificity; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Spectroscopy, Fourier Transform Infrared / methods
TL;DR: The ability of MALDESI-QFT-ICR MS to directly analyze and provide high mass measurement accuracy (approximately 1 part-per-million) of a polypeptide using internal calibration is demonstrated and the potential of this system for the top-down characterization of multiply charged polyPEptide cations is shown. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 journal article

A method for automatically interpreting mass spectra of 180-labeled isotopic clusters

MOLECULAR & CELLULAR PROTEOMICS, 6(2), 305–318.

MeSH headings : Algorithms; Chromatography, High Pressure Liquid; Cluster Analysis; Computational Biology; Mass Spectrometry / methods; Oxygen Isotopes / metabolism; Peptide Mapping; Proteome / metabolism; Proteomics / methods; Regression Analysis
TL;DR: An algorithm entitled regression analysis applied to mass spectrometry (RAAMS) is developed that automatically, rapidly, and confidently interprets spectra of 18O-labeled peptides without requiring chemical composition information derived from product ion spectra. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 article

New cavity rules

Yang, H. B., Hawkridge, A. M., Huang, S. P. D., Das, N., Bunge, S. D., Muddiman, D., & Stang, P. J. (2007, March). NATURE MATERIALS, Vol. 6, pp. 171–171.

MeSH headings : Alkynes / chemical synthesis; Alkynes / chemistry; Crystallography, X-Ray; Dendrimers / chemical synthesis; Dendrimers / chemistry; Fourier Analysis; Magnetic Resonance Spectroscopy; Models, Molecular; Organometallic Compounds / chemical synthesis; Organometallic Compounds / chemistry; Organoplatinum Compounds / chemical synthesis; Organoplatinum Compounds / chemistry; Spectrometry, Mass, Electrospray Ionization
TL;DR: Isotopically resolved mass spectrometry data support the existence of the metallodendrimers with rhomboidal and hexagonal cavities, and NMR data are consistent with the formation of all ensembles. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 journal article

Effect of matrix crystal structure on ion abundance of carbohydrates by matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 21(5), 807–811.

By: T. Williams n, D. Saggese n, R. Wilcox n, J. Martin n & D. Muddiman n

Contributors: T. Williams n, D. Saggese n, R. Wilcox n, J. Martin n & D. Muddiman n

MeSH headings : Acetylglucosamine / chemistry; Crystallization; Cyclotrons; Ions; N-Acetylneuraminic Acid / chemistry; Powder Diffraction; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Spectroscopy, Fourier Transform Infrared
TL;DR: Sample preparation techniques for carbohydrate analysis using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) are explored, with particular emphasis on analyte/matrix co-crystallization procedures. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2007 journal article

Sub parts-per-million mass measurement accuracy of intact proteins and product ions achieved using a dual electrospray ionization quadrupole Fourier transform ion cyclotron resonance mass spectrometer

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 18(1), 1–7.

By: K. Williams n, A. Hawkridge n & D. Muddiman n

MeSH headings : Amino Acid Sequence; Calibration; Cyclotrons; Ions; Melitten / chemistry; Molecular Sequence Data; Nanotechnology; Polymers / chemistry; Propylene Glycols / chemistry; Proteomics; Reproducibility of Results; Spectrometry, Mass, Electrospray Ionization / instrumentation; Spectrometry, Mass, Electrospray Ionization / methods; Spectroscopy, Fourier Transform Infrared / instrumentation; Spectroscopy, Fourier Transform Infrared / methods; Tandem Mass Spectrometry; Ubiquitin / chemistry
TL;DR: While external calibration of protein fragments resulted in absolute MMA greater than 16 ppm, internal standardization significantly improved upon the MMA of both the intact proteins and their products ions which ranged from −2.0 ppm to 1.1 ppm. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

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