Molecular Education, Technology and Research Innovation Center

Works Published in 2020

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Displaying works 41 - 44 of 44 in total

Sorted by most recent date added to the index first, which may not be the same as publication date order.

2020 journal article

Characterization of Dinophysis spp. (Dinophyceae, Dinophysiales) from the mid-Atlantic region of the United States

JOURNAL OF PHYCOLOGY, 56(2), 404–424.

By: J. Wolny*, T. Egerton*, S. Handy*, W. Stutts*, J. Smith*, E. Whereat*, T. Bachvaroff*, D. Henrichs*, L. Campbell*, J. Deeds*

author keywords: diarrhetic shellfish poisoning; dinoflagellate; Dinophysis; dinophysistoxin; environmental clones; harmful algal bloom; okadaic acid; phylogeny
MeSH headings : Dinoflagellida / genetics; Gulf of Mexico; Marine Toxins; Mediterranean Sea; Mid-Atlantic Region
UN Sustainable Development Goal Categories
14. Life Below Water (Web of Science; OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: February 27, 2020

2020 journal article

Investigating host-pathogen meta-metabolic interactions of Magnaporthe oryzae infected barley using infrared matrix-assisted laser desorption electrospray ionization mass spectrometry

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 412(1), 139–147.

By: J. Kalmar n, Y. Oh n, R. Dean n & D. Muddiman n

author keywords: IR-MALDESI; Mass spectrometry imaging; Orbitrap; Magnaporthe oryzae; Image recognition; Polarity switching
MeSH headings : Hordeum / metabolism; Hordeum / microbiology; Host-Pathogen Interactions; Infrared Rays; Magnaporthe / metabolism; Magnaporthe / physiology; Metabolomics; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR: Serotonin, a barley defense metabolite, was a compound identified in both positive and negative modes, and metabolites in the melanin pathway, important for infection development of M. oryzae were identified but were unable to be confirmed with MS/MS due to their low abundances. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: December 9, 2019

2020 journal article

Targeted capture of Chinese hamster ovary host cell proteins: Peptide ligand binding by proteomic analysis

BIOTECHNOLOGY AND BIOENGINEERING, 117(2), 438–452.

By: R. Lavoie n, A. Fazio n, T. Williams n, R. Carbonell n & S. Menegatti n

Contributors: R. Lavoie n, A. Fazio n, T. Williams n, R. Carbonell n & S. Menegatti n

author keywords: host cell proteins; monoclonal antibodies; polishing; peptide ligands; protein purification
MeSH headings : Animals; Antibodies, Monoclonal / isolation & purification; CHO Cells; Cricetinae; Cricetulus; Ligands; Peptides / chemistry; Peptides / metabolism; Protein Binding; Proteins / analysis; Proteins / chemistry; Proteins / isolation & purification; Proteins / metabolism; Proteomics / methods; Recombinant Proteins / isolation & purification
TL;DR: A family of synthetic peptides that target HCPs and exhibit low binding to IgG product are developed and found that these peptide ligands exhibit superior HCP binding capability compared to those of the benchmark commercial resins commonly used in mAb purification. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: December 2, 2019

2020 journal article

DRoP: Automated detection of conserved solvent-binding sites on proteins

PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 88(1), 152–165.

By: B. Kearney n, M. Schwabe*, K. Marcus*, D. Roberts n, M. Dechene n, P. Swartz n, C. Mattos n

author keywords: conserved solvent positions; DRoP software; protein hydration; protein multiple crystal structures; RNase A; water analysis program
MeSH headings : Animals; Binding Sites; Cattle; Crystallography, X-Ray; Databases, Protein; Humans; Ligands; Models, Molecular; Organic Chemicals / chemistry; Protein Binding; Protein Conformation; Proteins / chemistry; Ribonuclease, Pancreatic / chemistry; Software; Solvents / chemistry; Water / chemistry
TL;DR: A previously unpublished set of MSCS structures of bovine pancreatic ribonuclease A (RNase A) is presented and used together with published structures to illustrate the capabilities of DRoP, a flexible tool developed to automatically organize and rank the water or small organic molecule binding sites within a given set of structures. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 12, 2019

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