Works Published in 2010
Displaying works 41 - 60 of 117 in total
Sorted by most recent date added to the index first, which may not be the same as publication date order.
2010 journal article
Selection of DNA aptamer against prostate specific antigen using a genetic algorithm and application to sensing
Biosensors & Bioelectronics, 26(4), 1386–1391.
Contributors: N. Savory *, K. Abe *, K. Sode* & K. Ikebukuro *
author keywords: DNA aptamer; Prostate specific antigen; Genetic algorithm; In silk maturation
MeSH headings : Algorithms; Aptamers, Nucleotide / chemistry; Aptamers, Nucleotide / genetics; Aptamers, Nucleotide / isolation & purification; Base Sequence; Biosensing Techniques / methods; Biosensing Techniques / statistics & numerical data; Gene Library; Humans; Male; Nucleic Acid Conformation; Prostate-Specific Antigen / analysis; Prostate-Specific Antigen / blood; Prostatic Neoplasms / blood; Prostatic Neoplasms / diagnosis; SELEX Aptamer Technique
TL;DR:
The first report of a DNA aptamer against PSA and its application to PSA sensing is reported, with the aptamer showing a 48-fold higher PSA-binding ability than candidates obtained by SELEX.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2010 journal article
Pyrroloquinoline quinone inhibits the fibrillation of amyloid proteins.
Prion, 4(1), 26–31. http://www.scopus.com/inward/record.url?eid=2-s2.0-77949286151&partnerID=MN8TOARS
Contributors: J. Kim, M. Kobayashi, M. Fukuda, D. Ogasawara, N. Kobayashi, S. Han, C. Nakamura, M. Inada
Source: ORCID
Added: October 9, 2020
2010 journal article
Screening of DNA aptamer which binds to alpha-synuclein
Biotechnology Letters, 32(5), 643–648.
Contributors: K. Tsukakoshi *, R. Harada *, K. Sode* & K. Ikebukuro *
author keywords: alpha-Synuclein; Alzheimer's disease; DNA aptamer; Lewy bodies; Parkinson's disease
MeSH headings : Aptamers, Nucleotide / isolation & purification; Aptamers, Nucleotide / metabolism; Drug Evaluation, Preclinical / methods; Electrophoresis, Polyacrylamide Gel; Electrophoretic Mobility Shift Assay; Humans; Protein Binding; alpha-Synuclein / metabolism
TL;DR:
The first DNA aptamers against α-synuclein using native PAGE applied to the SELEX method are identified and this aptamer is called “M5-15”; it is the α- synuclein-bound aptamer and was isolated after four cycles of screening.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2010 journal article
Screening and Improvement of an Anti-VEGF DNA Aptamer
Molecules, 15(1), 215–225.
Contributors: Y. Nonaka*, K. Sode* & K. Ikebukuro *
author keywords: aptamer; cancer diagnosis; sensor element; VEGF(121); VEGF(165)
MeSH headings : Aptamers, Nucleotide / analysis; Aptamers, Nucleotide / genetics; Base Sequence; Circular Dichroism; Humans; Kinetics; Molecular Sequence Data; Mutant Proteins / metabolism; SELEX Aptamer Technique / methods; Surface Plasmon Resonance; Vascular Endothelial Growth Factor A / metabolism
www.mdpi.com (publisher PDF)
Source: ORCID
Added: October 9, 2020
2010 journal article
Pyrroloquinoline quinone inhibits the fibrillation of amyloid proteins
Prion, 4(1).
Contributors: J. Kim *, M. Kobayashi *, M. Fukuda*, D. Ogasawara *, N. Kobayashi *, S. Han*, C. Nakamura *, M. Inada *
author keywords: amyloid beta; amyloid fibril; cytotoxicity; fibril formation; inhibitor; prion; pyrroloquinoline quinone
MeSH headings : Amyloid / chemistry; Amyloid / metabolism; Animals; Humans; Mice; Molecular Structure; Neurodegenerative Diseases / drug therapy; PQQ Cofactor / chemistry; PQQ Cofactor / pharmacology; PQQ Cofactor / therapeutic use
TL;DR:
Pyrroloquinoline quinone (PQQ) inhibits theAmyloid fibril formation of the amyloid proteins, amyloids β (1-42) and mouse prion protein and this compound may become a leading anti-neurodegeneration compound in the treatment of neurodegenerative diseases.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2010 journal article
Pyrroloquinoline quinone inhibits the fibrillation of amyloid proteins
Prion, 4(1), 26–31. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000277180900005&KeyUID=WOS:000277180900005
Contributors: J. Kim, M. Kobayashi, M. Fukuda, D. Ogasawara, N. Kobayashi, S. Han, C. Nakamura, M. Inada
Source: ORCID
Added: October 9, 2020
2010 journal article
Motif-Based Search for a Novel Fructosyl Peptide Oxidase From Genome Databases
Biotechnology and Bioengineering, 106(3), 358–366.
Contributors: S. Kim*, S. Ferri*, W. Tsugawa *, K. Mori* & K. Sode*
author keywords: biosensor; diabetes; hemoglobin Alc (HbA1c); fructosyl amino acid oxidase (FAOD); fructosyl peptide oxidase (FPOX)
MeSH headings : Amino Acid Motifs; Amino Acid Oxidoreductases / genetics; Amino Acid Oxidoreductases / metabolism; Amino Acid Sequence; Ascomycota / enzymology; Ascomycota / genetics; Base Sequence; Cloning, Molecular; Computational Biology / methods; Databases, Nucleic Acid; Escherichia coli; Fungal Proteins / genetics; Genome, Fungal; Kinetics; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Structure, Tertiary; Recombinant Proteins / genetics; Recombinant Proteins / isolation & purification; Recombinant Proteins / metabolism; Sequence Alignment; Substrate Specificity
TL;DR:
The gene encoding a putative fructosyl amino acid oxidase was found in the Phaeosphaeria nodorum SN15 genome and successfully expressed in Escherichia coli, and Gly60 was found to be a determining residue for the activity towards f‐αVal‐His.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2010 journal article
Functional expression of Phanerochaete chrysosporium cellobiose dehydrogenase flavin domain in Escherichia coli
Biotechnology Letters, 32(6), 855–859.
Contributors: Desriani, S. Ferri* & K. Sode*
author keywords: Cellobiose; Cellobiose dehydrogenase; Flavin domain; Phanerochaete chrysosporium
MeSH headings : Carbohydrate Dehydrogenases / biosynthesis; Carbohydrate Dehydrogenases / genetics; Cellobiose / metabolism; Enzyme Stability; Escherichia coli / genetics; Escherichia coli / metabolism; Gene Expression; Hydrogen-Ion Concentration; Phanerochaete / enzymology; Phanerochaete / genetics; Pichia / genetics; Pichia / metabolism; Protein Structure, Tertiary; Recombinant Proteins / biosynthesis; Recombinant Proteins / genetics
TL;DR:
The soluble flavin domain of the Phanerochaete chrysosporium CDH was successfully expressed in Escherichia coli and the enzyme showed dye-mediated CDH activity higher than that of the complete CDH, composed of flavindomain and heme domain.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2010 journal article
Engineering of dye-mediated dehydrogenase property of fructosyl amino acid oxidases by site-directed mutagenesis studies of its putative proton relay system
Biotechnology Letters, 32(8), 1123–1129.
Contributors: S. Kim*, E. Nibe*, S. Ferri*, W. Tsugawa * & K. Sode*
author keywords: Biosensor; Dehydrogenase; Flavoprotein; Fructosyl amino acid oxidase (FAOD); Glycated protein; Proton relay system (PRS)
MeSH headings : Amino Acid Oxidoreductases / chemistry; Amino Acid Oxidoreductases / genetics; Amino Acid Oxidoreductases / metabolism; Amino Acid Sequence; Aspergillus / enzymology; Cloning, Molecular; Crystallography, X-Ray; Electrophoresis, Polyacrylamide Gel; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Oxidoreductases / chemistry; Oxidoreductases / genetics; Oxidoreductases / metabolism; Pichia / enzymology; Protons
TL;DR:
To alter their electron acceptor availability, site-directed mutagenesis was carried out on conserved residues predicted to be involved in the proton relay system (PRS) of two eukaryotic FAODs, the FAOD from the marine yeast Pichia sp.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2010 journal article
Constructing an improved pyrroloquinoline quinone glucose dehydrogenase binding aptamer for enzyme labeling
Biotechnology Letters, 32(9), 1293–1298.
Contributors: K. Abe *, K. Sode* & K. Ikebukuro *
author keywords: DNA aptamer; Electrochemical biosensors; Pyrroloquinoline quinone-dependent glucose dehydrogenase; G-quadruplex
MeSH headings : Aptamers, Nucleotide / metabolism; Glucose Dehydrogenases / metabolism; Protein Binding; Staining and Labeling / methods
TL;DR:
Because the truncated mutants minimized unexpected interactions between PGa4 and the target- binding aptamer, they will now facilitate the construction of a highly sensitive detection system using a PQQGDH-labeled diagnostic marker-binding aptamer.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2010 journal article
Amino acid substitution at the substrate-binding subsite alters the specificity of the Phanerochaete chrysosporium cellobiose dehydrogenase
Biochemical and Biophysical Research Communications, 391(2), 1246–1250.
Contributors: Desriani, S. Ferri* & K. Sode*
author keywords: Cellobiose dehydrogenase; Cellobiose; Lactose; Phanerochaete chrysosporium
MeSH headings : Amino Acid Substitution; Binding Sites / genetics; Carbohydrate Dehydrogenases / chemistry; Carbohydrate Dehydrogenases / genetics; Carbohydrate Dehydrogenases / metabolism; Flavins / metabolism; Lactose / metabolism; Phanerochaete / enzymology; Protein Structure, Tertiary; Substrate Specificity / genetics
TL;DR:
While the wild-typecellobiose dehydrogenase efficiently oxidizes cellobiose and lactose, the Glu279Gln mutant retained most of its activity with cellobioses but was completely inactive with lactose.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2010 chapter
Chapter 70: Evolution of Biofeedback in Physical Medicine and Rehabilitation
In W. Frontera (Ed.), DeLisa’s Physical Medicine and Rehabilitation: Principles and Practice (5th ed.). Philadelphia: Lippincott Williams & Wilkins.
Ed(s): W. Frontera
Source: NC State University Libraries
Added: September 24, 2020
2010 journal article
Selective isolation of live/dead cells using contactless dielectrophoresis (cDEP)
Lab on a Chip, 10(4), 438.
MeSH headings : Animals; Cell Death; Cell Line, Tumor; Cell Separation / instrumentation; Cell Separation / methods; Cell Survival; Electric Conductivity; Electrophoresis; Humans
TL;DR:
Two microfluidic devices capable of selectively isolating live human leukemia cells from dead cells utilizing their electrical signatures are presented, achieving greater than 95% removal efficiency and 100% selectivity between live and dead cells.
(via Semantic Scholar)
Source: Crossref
Added: August 28, 2020
2010 journal article
Electromagnetically Controlled Biological Assembly of Aligned Bacterial Cellulose Nanofibers
Annals of Biomedical Engineering, 38(8), 2475–2484.
author keywords: Acetobacter xylinum; Biological assembly; Directed biofabrication; Electrokinetics
MeSH headings : Air; Bacteria; Cellulose / biosynthesis; Cellulose / ultrastructure; Culture Media; Electricity; Gluconacetobacter xylinus / metabolism; Magnetics; Nanofibers; Oxygen / metabolism
TL;DR:
A new biofabrication process in which the precise control of bacterial motion is used to fabricate customizable networks of cellulose nanofibrils, demonstrating a new concept of bottom up material synthesis by the control of a biological assembly process.
(via Semantic Scholar)
Source: Crossref
Added: August 28, 2020
2010 journal article
Altered digit force direction during pinch grip following stroke
Experimental Brain Research, 202(4), 891–901.
author keywords: Digit force; Force direction; Force vector; Stroke; Prehension; Pinch grip
MeSH headings : Adult; Biomechanical Phenomena; Chronic Disease; Electromyography; Fingers / physiology; Functional Laterality; Hand Strength / physiology; Humans; Middle Aged; Motor Skills / physiology; Muscle, Skeletal / physiology; Paresis / physiopathology; Regression Analysis; Stroke / physiopathology; Task Performance and Analysis; Time Factors
TL;DR:
Therapies to redirect the force direction at the digits may improve stroke survivors’ ability to stably grip an object.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: Crossref
Added: August 28, 2020
2010 journal article
Effect of diffusion on impedance measurements in a hydrodynamic flow focusing sensor
Lab on a Chip, 10(20), 2787.
MeSH headings : Conductometry / instrumentation; Diffusion; Electric Impedance; Equipment Design; Equipment Failure Analysis; Escherichia coli / chemistry; Escherichia coli / physiology; Microfluidic Analytical Techniques / instrumentation; Transducers
TL;DR:
The results showed that the diffusion mass transport at the interface of the two streams limited the benefits of increased flow focusing, and increasing flow velocities could be used to offset the diffusion effect.
(via Semantic Scholar)
Source: Crossref
Added: August 28, 2020
2010 journal article
Dynamic reversibility of hydrodynamic focusing for recycling sheath fluid
Lab on a Chip, 10(15), 1952.
MeSH headings : Flow Cytometry / instrumentation; Flow Cytometry / methods; Microfluidic Analytical Techniques / instrumentation; Microfluidic Analytical Techniques / methods; Microfluidics / instrumentation; Microfluidics / methods; Microspheres; Models, Theoretical; Polystyrenes / chemistry
Source: Crossref
Added: August 28, 2020
2010 journal article
Hydrodynamic focusing of conducting fluids for conductivity-based biosensors
Biosensors and Bioelectronics, 25(6), 1363–1369.
author keywords: Conductivity; Flow-focusing; Biosensor; Reynolds number; Magnetic beads; Hydrodynamic focusing
MeSH headings : Biosensing Techniques / instrumentation; Computer-Aided Design; Electric Conductivity; Electrochemistry / instrumentation; Equipment Design; Equipment Failure Analysis; Flow Injection Analysis / instrumentation; Microfluidic Analytical Techniques / instrumentation; Pressure; Solutions / chemistry
TL;DR:
Computational simulations conducted in conjunction with experimental flow studies using confocal microscopy show that a flat profile for the focused layer is dependent on the Reynolds number for the chosen flow parameters, and results indicate that a flattening layer is desirable for both increased sensitivity as well as surface-binding efficiency.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(OpenAlex)
Source: Crossref
Added: August 28, 2020
2010 journal article
Multiplexed magnetic microsphere immunoassays for detection of pathogens in foods
Sensing and Instrumentation for Food Quality and Safety, 4(2), 73–81.
TL;DR:
While the magnetic and fluorescent properties of the Luminex MagPlex microspheres allow for rapid, multiplexed testing for bacterial contamination in typically problematic food matrices, the data demonstrate that achieving desired limits of detection is still a challenge.
(via Semantic Scholar)
Source: Crossref
Added: August 28, 2020
2010 journal article
A hard microflow cytometer using groove-generated sheath flow for multiplexed bead and cell assays
Analytical and Bioanalytical Chemistry, 398(5), 1871–1881.
author keywords: Flow cytometry; Microflow cytometer; Hydrodynamic focusing
MeSH headings : Biological Assay / instrumentation; Biological Assay / methods; Cell Line, Tumor; Flow Cytometry / instrumentation; Flow Cytometry / methods; Humans; Immunoassay; Microspheres; Polymethyl Methacrylate / chemistry
TL;DR:
The hard microflow cytometer is a robust device that can operate at high pressures that could withstand pressures greater than 100 psi without leaking.
(via Semantic Scholar)
Source: Crossref
Added: August 28, 2020
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