Works Published in 2019
Displaying works 21 - 40 of 48 in total
Sorted by most recent date added to the index first, which may not be the same as publication date order.
2019 journal article
Genome editing using the endogenous type I CRISPR-Cas system in Lactobacillus crispatus
Proceedings of the National Academy of Sciences.
author keywords: CRISPR; Cas3; type I-E; genome editing; probiotic
MeSH headings : Base Sequence; CRISPR-Cas Systems / genetics; Gene Editing; Genetic Loci; Genetic Variation; Lactobacillus crispatus / genetics; Lactobacillus crispatus / ultrastructure; Nucleotide Motifs / genetics; RNA, Guide, Kinetoplastida / genetics
TL;DR:
This approach enables flexible alteration of the formerly genetically recalcitrant species L. crispatus, with potential for probiotic enhancement, biotherapeutic engineering, and mucosal vaccine delivery, and the toolbox is expanded to include one of the most abundant and diverse systems found in nature.
(via Semantic Scholar)
Source: ORCID
Added: January 2, 2020
2019 journal article
Targeted transcriptional modulation with type I CRISPR-Cas systems in human cells
NATURE BIOTECHNOLOGY, 37(12), 1493-+.
MeSH headings : CRISPR-Cas Systems / genetics; Escherichia coli / genetics; Genetic Engineering / methods; HEK293 Cells; Humans; Listeria monocytogenes / genetics; RNA, Guide, Kinetoplastida / genetics; Transcription, Genetic / genetics
TL;DR:
This study repurposes type I variants of class 1 CRISPR–Cas systems from Escherichia coli and Listeria monocytogenes, which target DNA via a multi-component RNA-guided complex termed Cascade, and demonstrates the use of Cascade as a CRISpr-based technology for targeted eukaryotic gene regulation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: December 30, 2019
2019 article
Predicting and visualizing features of CRISPR-Cas systems
CRISPR-CAS ENZYMES, Vol. 616, pp. 1–25.
MeSH headings : Animals; Bacteria / genetics; CRISPR-Cas Systems; Clustered Regularly Interspaced Short Palindromic Repeats; Computer Simulation; Gene Editing / methods; Genomics / methods; Humans; RNA, Guide, Kinetoplastida / genetics; Software
TL;DR:
In silico methods for predicting and visualizing notable features of CRISPR-Cas systems, including Cas domain determination,CRISPR array visualization, and inference of the protospacer-adjacent motif are described.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: December 30, 2019
2019 journal article
Comparative genomics of eight Lactobacillus buchneri strains isolated from food spoilage
BMC GENOMICS, 20(1).
author keywords: Lactobacillus buchneri; Comparative genomics; Lactic acid bacteria; CRISPR-Cas systems; Fermentation; Spoilage; Food microbiology
MeSH headings : Computational Biology / methods; Evolution, Molecular; Fermentation; Food Microbiology; Genome, Bacterial; Genomics / methods; Lactobacillus / classification; Lactobacillus / genetics; Lactobacillus / isolation & purification; Molecular Sequence Annotation; Phylogeny; Whole Genome Sequencing
TL;DR:
The findings from the comparison of eight newly-sequenced and assembled genomes against two publicly available closed reference genomes provide new insights into the genomics of L. buchneri, building a platform for future studies and identifying elements for potential strain manipulation or engineering.
(via Semantic Scholar)
bmcgenomics.biomedcentral.com (publisher PDF)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: December 16, 2019
2019 article
Casein as a Modifier of Whey Protein Isolate Gel: Sensory Texture and Rheological Properties
JOURNAL OF FOOD SCIENCE.
MeSH headings : Caseins / chemistry; Caseins / metabolism; Elasticity; Gels / chemistry; Gels / metabolism; Hot Temperature; Humans; Mastication; Rheology; Taste; Whey Proteins / chemistry; Whey Proteins / metabolism
TL;DR:
It was demonstrated that casein can be used to alter whey protein gel structure such that sensory firmness and fracturability are decreased and cohesiveness is increased, while preventing a large increase in moisture release.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: December 2, 2019
2019 article
Collaborative networks in gene editing
Huang, Y., Porter, A., Zhang, Y., & Barrangou, R. (2019, October). NATURE BIOTECHNOLOGY, Vol. 37, pp. 1107–1109.
MeSH headings : Biomedical Research / trends; Gene Editing; Humans; International Cooperation; Leadership; Publishing / trends
TL;DR:
Of the literature on gene-editing modalities, CRISPR–Cas continues to grow, whereas publications describing meganucleases, zinc finger nucleases and transcription activator–like effector nuclease (TALENs) have been decreasing since 2016.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: November 18, 2019
2019 journal article
Use of acid whey protein concentrate as an ingredient in nonfat cup set-style yogurt
JOURNAL OF DAIRY SCIENCE, 102(10), 8768–8784.
author keywords: acid whey; sweet whey; yogurt
MeSH headings : Animals; Cheese / analysis; Fermentation; Food Handling / methods; Food Ingredients; Milk / chemistry; Milk Proteins / analysis; Pasteurization; Taste; Whey / chemistry; Whey Proteins / chemistry; Yogurt / analysis
TL;DR:
Differences in pH was the main contributor to texture differences, as higher pH in acid whey protein yogurts changed gel structure formation and water-holding capacity of the yogurt gel, which translated to differences in trained panel texture attributes and lower consumer liking scores for fat-free yogurt made with added acid Whey protein ingredient.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: October 7, 2019
2019 journal article
Effects of milk fat, casein, and serum protein concentrations on sensory properties of milk-based beverages
JOURNAL OF DAIRY SCIENCE, 102(10), 8670–8690.
author keywords: casein; serum protein; milk-based beverage
MeSH headings : Adult; Animals; Beverages / analysis; Blood Proteins / analysis; Caseins; Cattle; Color; Female; Glycolipids / analysis; Glycoproteins / analysis; Humans; Lipid Droplets; Male; Middle Aged; Milk / chemistry; Milk Proteins / analysis; Particle Size; Pasteurization; Taste; Viscosity
TL;DR:
Understanding the expected relative effect of different milk protein ingredients on the textural and flavor characteristics of milk-based beverages could be used to help guide product reformulation decisions and ingredient choices to achieve a specific sensory profile while controlling total beverage ingredient cost.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: October 7, 2019
2019 journal article
Identification of the source of volatile sulfur compounds produced in milk during thermal processing
JOURNAL OF DAIRY SCIENCE, 102(10), 8658–8669.
author keywords: skim milk; volatile sulfur compound; ultrapasteurization
MeSH headings : Animals; Caseins / analysis; Cattle; Gas Chromatography-Mass Spectrometry; Hot Temperature; Milk / chemistry; Milk Proteins / analysis; Odorants / analysis; Pasteurization / methods; Solid Phase Microextraction; Steam; Sulfides; Sulfur Compounds / analysis; Taste
TL;DR:
The combination of sensory and instrumental methods used in the current study effectively identified that milk serum proteins are the source of sulfur compounds in milk, and further confirmed the contribution of hydrogen sulfide and carbon disulfide to eggy and sulfur/burnt flavors, respectively.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Source: Web Of Science
Added: October 7, 2019
2019 journal article
Hunting interacts with socio-demographic predictors of human perceptions of urban coyotes
WILDLIFE SOCIETY BULLETIN, 43(3), 447–454.
author keywords: Canis latrans; carnivore; coyotes; hunting; moderating effects; urban
UN Sustainable Development Goal Categories
11. Sustainable Cities and Communities
(OpenAlex)
15. Life on Land
(Web of Science)
Source: Web Of Science
Added: September 16, 2019
2019 journal article
Outcomes and characterization of chromosomal self-targeting by native CRISPR-Cas systems in Streptococcus thermophilus
FEMS MICROBIOLOGY LETTERS, 366(9).
author keywords: CRISPR; lactic acid bacteria; Cas9; Streptococcus; genome editing
MeSH headings : Bacteriophages / genetics; CRISPR-Cas Systems; Chromosomes, Bacterial / genetics; Gene Editing; Genome, Bacterial; Homologous Recombination; Plasmids / genetics; Streptococcus thermophilus / genetics; Streptococcus thermophilus / virology
TL;DR:
Results suggest that HR contributes to the plasticity and remodeling of bacterial genomes, leading to evasion of genome targeting by CRISPR-Cas systems.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: September 3, 2019
2019 journal article
Short communication: Transcriptional response to a large genomic island deletion in the dairy starter culture Streptococcus thermophilus
JOURNAL OF DAIRY SCIENCE, 102(9), 7800–7806.
author keywords: fermentation; Streptococcus thermophilus; transcriptome; CRISPR
MeSH headings : Animals; Clustered Regularly Interspaced Short Palindromic Repeats; Fermentation; Gene Deletion; Gene Expression Regulation, Bacterial / physiology; Genomic Islands; Genomics; Lactic Acid / metabolism; Lactobacillus delbrueckii / metabolism; Milk / microbiology; Streptococcus thermophilus / genetics; Streptococcus thermophilus / metabolism; Transcriptome; Yogurt / microbiology
TL;DR:
Results suggest that deletion of a 102-kb genomic island in Strep.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
14. Life Below Water
(OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 26, 2019
2019 journal article
Consumer insights on prepackaged Cheddar cheese shreds using focus groups, conjoint analysis, and qualitative multivariate analysis
JOURNAL OF DAIRY SCIENCE, 102(8), 6971–6986.
author keywords: Cheddar cheese shreds; consumer liking; conjoint analysis
MeSH headings : Cheese / analysis; Cheese / economics; Choice Behavior; Color; Commerce; Consumer Behavior; Focus Groups; Food Packaging; Multivariate Analysis; Surveys and Questionnaires; Taste
TL;DR:
Qualitative multivariate analysis results confirmed the focus group and survey results: meltability, orange color, lack of clumps, ability to reseal the bag, and desirable "Cheddar" flavor were also preferred Cheddar shred qualities.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: July 29, 2019
2019 journal article
Does vitamin fortification affect light oxidation in fluid skim milk?
JOURNAL OF DAIRY SCIENCE, 102(6), 4877–4890.
author keywords: Vitamin fortification; light oxidation; milk; flavor
MeSH headings : Animals; Cattle; Food Handling / methods; Food, Fortified; Gas Chromatography-Mass Spectrometry; Light; Milk / chemistry; Milk / radiation effects; Odorants; Oxidation-Reduction; Pasteurization; Riboflavin / chemistry; Solid Phase Microextraction; Taste; Vitamin A / chemistry; Vitamin D / chemistry; Vitamins / chemistry
TL;DR:
The results show no evidence that vitamin fortification at current levels provides any protection against light oxidation-related off-flavors in fluid milk and a perfumey/floral flavor was documented in the oil-based vitamin A-fortified milk, suggesting that light exposure affected the off-Flavors contributed by water- versus oil- baseditamin fortification.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Source: Web Of Science
Added: June 24, 2019
2019 journal article
Understanding implicit and explicit consumer desires for protein bars, powders, and beverages
JOURNAL OF SENSORY STUDIES, 34(3).
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: June 24, 2019
2019 journal article
Short communication: Comparison of 3 rapid methods for analysis of vitamin degradation compounds in fluid skim milk
JOURNAL OF DAIRY SCIENCE, 102(6), 4906–4912.
author keywords: milk; vitamin degradation product; solid-phase microextraction; stir bar sorptive extraction
MeSH headings : Adsorption; Animals; Flavoring Agents; Milk / chemistry; Pasteurization; Reproducibility of Results; Solid Phase Microextraction; Vitamins / analysis; Vitamins / chemistry
TL;DR:
Based on linearity, relative standard deviation, and limits of detection and quantitation, cyclohexane solvent-assisted stir bar sorptive extraction with 10-mL sample volume is recommended for the quantitation of vitamin degradation-related volatiles in fluid skim milk.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: June 24, 2019
2019 journal article
Recombination between phages and CRISPR-cas loci facilitates horizontal gene transfer in staphylococci
NATURE MICROBIOLOGY, 4(6), 956–963.
MeSH headings : Bacteria / genetics; Bacteriophages / genetics; CRISPR-Cas Systems; Clustered Regularly Interspaced Short Palindromic Repeats / genetics; Clustered Regularly Interspaced Short Palindromic Repeats / immunology; Endonucleases; Gene Transfer, Horizontal; Genome, Bacterial; Genome, Viral; Plasmids / genetics; Pseudomonas aeruginosa / genetics; Sequence Analysis, DNA; Staphylococcus / genetics; Staphylococcus aureus / genetics; Transduction, Genetic
TL;DR:
The data demonstrate that spacer sequences not only specify the targets of Cas nucleases but also can promote horizontal gene transfer, which can promote transfer of CRISPR elements to other bacteria in the population.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: June 17, 2019
2019 review
Harnessing CRISPR-Cas systems for precision engineering of designer probiotic lactobacilli
[Review of ]. CURRENT OPINION IN BIOTECHNOLOGY, 56, 163–171.
MeSH headings : CRISPR-Cas Systems / genetics; Drug Delivery Systems; Gene Editing; Genetic Engineering / methods; Lactobacillus / genetics; Probiotics / metabolism
TL;DR:
How the rising adoption of CRISPR-based technologies for prokaryotic engineering and tunable transcriptional regulation can be translated into next-generation development of probiotic lactobacilli with enhanced robustness and designer functionalities is discussed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: June 17, 2019
2019 review
CRISPR-Directed Microbiome Manipulation across the Food Supply Chain
[Review of ]. TRENDS IN MICROBIOLOGY, 27(6), 489–496.
MeSH headings : CRISPR-Cas Systems; Fermentation; Food Chain; Food Microbiology; Food Supply; Gene Editing; Metabolic Engineering; Microbiota
TL;DR:
It is illustrated how the various CRISPR technologies that can alter bacterial functionalities and modulate the composition of microbial communities in foods can be harnessed along the food supply chain to manipulate microbiomes that encompass spoilage and pathogenic bacteria as well as desirable starter cultures and health-promoting probiotics.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: June 4, 2019
2019 journal article
Comparative genomics and evolution of trans-activating RNAs in Class 2 CRISPR-Cas systems
RNA BIOLOGY, 16(4), 435–448.
MeSH headings : Bacteroides / genetics; Base Sequence; CRISPR-Cas Systems / genetics; Conserved Sequence / genetics; Evolution, Molecular; Genomics; Nucleic Acid Conformation; RNA, Bacterial / genetics; RNA, Guide, Kinetoplastida / genetics; Repetitive Sequences, Nucleic Acid / genetics; Streptococcus / genetics; Thermodynamics; Trans-Activators / genetics
TL;DR:
Reconstruction of tracrRNA evolution for 4 tight bacterial groups demonstrates random drift of repeat-antirepeat complementarity within a window of hybrid stability that is, apparently, maintained by selection.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
15. Life on Land
(OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: May 20, 2019
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