Golden Leaf Biomanufacturing Training and Education Center

Works Published in 2022

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Displaying all 9 works

Sorted by most recent date added to the index first, which may not be the same as publication date order.

2022 journal article

Flexible sensor patch for continuous carbon dioxide monitoring


author keywords: CO2; in-line sensor; flexible sensor; process analytical technology; cell culture; biomanufacturing
Source: Web Of Science
Added: March 6, 2023

2022 journal article

Purification of Adeno-Associated Virus (AAV) Serotype 2 from Spodoptera frugiperda (Sf9) Lysate by Chromatographic Nonwoven Membranes

MEMBRANES, 12(10).

author keywords: adeno-associated virus (AAV); viral vector purification; nonwovens; membrane adsorber; anion exchange; cation exchange; membrane chromatography
TL;DR: A strong anion-exchange membrane was developed by UV grafting glycidyl methacrylate on a polybutylene terephthalate nonwoven followed by functionalization with triethylamine, resulting in a quaternary amine ligand (AEX-TEA membrane) that exhibited a high AAV2 binding capacity and achieved excellent DNA clearance and a recovery of infectivity higher that that reported using ion-ex exchange resin chromatography. (via Semantic Scholar)
Source: Web Of Science
Added: November 14, 2022

2022 journal article

Development of peptide ligands for the purification of a-1 antitrypsin from cell culture fluids


author keywords: a-1 antitrypsin; Peptide ligands; Affinity chromatography; Protein purification; CHO cell culture harvest
MeSH headings : Animals; CHO Cells; Cell Culture Techniques; Chromatography, Affinity; Cricetinae; Cricetulus; Ligands; Molecular Docking Simulation; Peptides; alpha 1-Antitrypsin
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: October 11, 2022

2022 article

Mobile On Demand COVID-19 Vaccine Production Units for Developing Countries

Fahr, S., Pena-Benavides, S. A., Thiel, L., Sengoba, C., Karacasulu, K., Ihling, N., … Roh, K. (2022, August 19). INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH, Vol. 61.

By: S. Fahr*, S. Pena-Benavides*, L. Thiel*, C. Sengoba*, K. Karacasulu*, N. Ihling*, J. Sosa-Hernandez*, G. Gilleskie n ...

Contributors: S. Fahr*, S. Pena-Benavides*, L. Thiel*, C. Sengoba*, K. Karacasulu*, N. Ihling*, J. Sosa-Hernandez*, G. Gilleskie n ...

TL;DR: This work designed two MOD vaccine manufacturing units based on a protein antigen expressed in yeast and in vitro transcription of mRNA that can produce on the order of 10,000 vaccine doses daily for competitive prices and in close proximity of their end users. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: ORCID, Web Of Science
Added: September 14, 2022

2022 article

Towards continuous mAb purification: Clearance of host cell proteins from CHO cell culture harvests via "flow-through affinity chromatography" using peptide-based adsorbents

Sripada, S. A., Chu, W., Williams, T. I., Teten, M. A., Mosley, B. J., Carbonell, R. G., … Menegatti, S. (2022, April 22). BIOTECHNOLOGY AND BIOENGINEERING.

By: S. Sripada n, W. Chu n, T. Williams n, M. Teten n, B. Mosley n, R. Carbonell n, A. Lenhoff*, S. Cramer* ...

author keywords: CHO; flow-through chromatography; host cell proteins; monoclonal antibodies; peptide-based adsorbents
MeSH headings : Animals; Antibodies, Monoclonal / chemistry; CHO Cells; Cell Culture Techniques; Chromatography, Affinity / methods; Cricetinae; Cricetulus; Peptides / chemistry; Proteomics / methods
TL;DR: An ensemble of peptide ligands that target the HCPs in Chinese hamster ovary (CHO) cell culture fluids and enable mAb purification via flow‐through affinity chromatography are introduced and their integration into LigaGuard™, an affinity adsorbent featuring an equilibrium binding capacity. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: May 10, 2022

2022 journal article

Epigenome-wide association study of bronchopulmonary dysplasia in preterm infants: results from the discovery-BPD program


By: X. Wang*, H. Cho*, M. Campbell*, V. Panduri*, S. Coviello*, M. Caballero*, D. Sambandan n, S. Kleeberger* ...

author keywords: Preterm infant; Cord blood; DNA methylation; Epigenome-wide association study; Gestational age; Stochastic epimutation; Nucleated red blood cell; cDNA microarray; Bronchopulmonary dysplasia; Lung
MeSH headings : Biomarkers; Birth Weight; Bronchopulmonary Dysplasia / genetics; DNA Methylation; Epigenome; Humans; Infant; Infant, Newborn; Infant, Premature
TL;DR: Results must be interpreted with caution because of the small size of this study, NRBC content strongly impacted DNA methylation profiles in preterm cord blood and EWAS analysis revealed potential insights into biological pathways involved in BPD pathogenesis. (via Semantic Scholar)
Source: Web Of Science
Added: May 10, 2022

2022 journal article

Using Proteomic Approaches to Unravel the Response of Ctenocephalides felis felis to Blood Feeding and Infection With Bartonella henselae


By: M. Andre n, P. Neupane n, M. Lappin*, B. Herrin*, V. Smith*, T. Williams n, L. Collins n, H. Bai n ...

author keywords: bartonellosis; cat flea; cat scratch disease; flea-pathogen interface; proteome
MeSH headings : Animals; Bartonella henselae / genetics; Cat Diseases; Cats; Ctenocephalides; Felis; Proteomics; Siphonaptera
TL;DR: Flea-differentially abundant proteins (FDAP) are investigated in unfed fleas, fleas fed on uninfected cats, and flea fed on B. henselae-infected cats to help understand the complex interactions between cat fleas and B. Henselae over an infection time course, thus helping understand the dual effect of vaccine targeting both flea fitness and pathogen competence. (via Semantic Scholar)
Source: Web Of Science
Added: March 7, 2022

2022 article

A direct comparison between membrane adsorber and packed column chromatography performance (vol 1612, 460629, 2020)

Boi, C., Malavasi, A., Carbonell, R. G., & Gilleskie, G. (2022, March 15). JOURNAL OF CHROMATOGRAPHY A, Vol. 1666.

By: C. Boi, A. Malavasi n, R. Carbonell n & G. Gilleskie n

Sources: Web Of Science, ORCID
Added: February 21, 2022

2022 journal article

A concept for continuous virus manufacture using a moving bed bioreactor: Growth of MDCK cells to confluence on paper as a model support


By: C. Duffy n, L. Overton n & M. Flickinger n

author keywords: Cell culture on paper; Moving bed bioreactor; Continuous virus manufacture; Intensification of vaccine manufacture; MDCK cell growth on paper
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: November 15, 2021

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