Works Published in 2011
Displaying works 21 - 33 of 33 in total
Sorted by most recent date added to the index first, which may not be the same as publication date order.
2011 journal article
Comparison of stable-isotope labeling with amino acids in cell culture and spectral counting for relative quantification of protein expression
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 25(17), 2524–2532.
MeSH headings : Amino Acid Sequence; Amino Acids / chemistry; Amino Acids / metabolism; Animals; Cell Differentiation; Cell Line; Ectoderm; Embryonic Stem Cells / cytology; Embryonic Stem Cells / metabolism; Humans; Isotope Labeling / methods; Mass Spectrometry; Mice; Molecular Sequence Data; Peptide Fragments; Peptide Mapping; Proteins / analysis; Proteins / chemistry; Proteins / metabolism; Proteomics / methods; Sensitivity and Specificity; Tandem Mass Spectrometry; Time Factors
TL;DR:
The comparison of stable-isotope labeling of amino acids in cell culture (SILAC) with spectral counting for the quantification of human embryonic stem cells as they differentiate toward the trophectoderm at three time points is presented.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2011 journal article
Increasing proteome coverage with offline RP HPLC coupled to online RP nanoLC-MS
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 879(9-10), 610–614.
author keywords: RP HPLC; Fractionation methods; Proteomics; Mass spectrometry
MeSH headings : Chromatography, High Pressure Liquid / methods; Chromatography, Reverse-Phase / methods; Mass Spectrometry / methods; Nanotechnology; Peptide Fragments / analysis; Peptide Fragments / metabolism; Proteome / analysis; Proteome / metabolism; Proteomics / methods; Saccharomyces cerevisiae Proteins / analysis; Saccharomyces cerevisiae Proteins / metabolism
TL;DR:
The success of performing offline reversed phase high pressure liquid chromatography (HPLC) fractionation on a C18 2.0 mm×150 mm column at the peptide level with microliter per minute flow rates prior to online nano-flow reversed phase liquid Chromatography mass spectrometry (nanoLC-MS) using the well-studied fungus Saccharomyces cerevisiae is reported.
(via Semantic Scholar)
europepmc.org (repository)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2011 journal article
Improving Proteome Coverage on a LTQ-Orbitrap Using Design of Experiments
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 22(4), 773–783.
author keywords: LTQ-Orbitrap XL MS/MS instrument parameters; Design of experiments; Fractional-factorial design; Saccharomyces cerevisiae; Proteomics
MeSH headings : Peptide Fragments; Peptide Mapping; Proteome / chemistry; Proteomics / methods; Research Design; Saccharomyces cerevisiae Proteins / chemistry; Tandem Mass Spectrometry / methods; Temperature
TL;DR:
The DOE approach represents a time- and cost-effective method for empirically optimizing MS-based proteomics workflows including sample preparation, LC conditions, and multiple instrument platforms.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2011 journal article
Identification of alternative splice variants in Aspergillus flavus through comparison of multiple tandem MS search algorithms
BMC Genomics, 12.
Source: NC State University Libraries
Added: August 6, 2018
2011 journal article
Hydrophobic Derivatization of N-linked Glycans for Increased Ion Abundance in Electrospray Ionization Mass Spectrometry
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 22(8), 1309–1317.
author keywords: Hydrophobic Derivatization; HILIC; nanoESI; N-Linked Glycans; Hydrazone Formation
MeSH headings : Blood Proteins / chemistry; Carbohydrate Sequence; Chromatography, Liquid; Glycoproteins / chemistry; Humans; Hydrazines / chemistry; Hydrophobic and Hydrophilic Interactions; Polysaccharides / chemistry; Small Molecule Libraries; Spectrometry, Mass, Electrospray Ionization / methods; Tandem Mass Spectrometry
TL;DR:
The results of this study will direct the future synthesis of hydrophobic reagents for glycan analysis using the correlation between hydrophobicity and theoretical non-polar surface area calculation to facilitate the development of an optimum tag for gly can derivatization.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2011 journal article
Facile Self-Assembly of Supramolecular Hexakisferrocenyl Triangles via Coordination-Driven Self-Assembly and Their Electrochemical Behavior
ORGANOMETALLICS, 30(13), 3637–3642.
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2011 review
Capitalizing on the hydrophobic bias of electrospray ionization through chemical modification in mass spectrometry-based proteomics
[Review of ]. EXPERT REVIEW OF PROTEOMICS, 8(3), 317–323.
author keywords: ALiPHAT; electrospray ionization; hydrophobic tagging; mass spectrometry; quantification
MeSH headings : Humans; Hydrophobic and Hydrophilic Interactions; Proteomics / methods; Spectrometry, Mass, Electrospray Ionization / methods
TL;DR:
Improvements in targeted, mass spectrometry-based proteomic strategies are achieved by taking advantage of the mechanism of ESI through the use of hydrophobic chemical derivatization.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2011 journal article
John B. Fenn special issue recognizing the science of Professor John B. Fenn foreword
International Journal of Mass Spectrometry, 300(2-3), 72–73.
Source: NC State University Libraries
Added: August 6, 2018
2011 journal article
Functional dissection of Odorant binding protein genes in Drosophila melanogaster
GENES BRAIN AND BEHAVIOR, 10(6), 648–657.
Contributors: S. Swarup*, T. Williams* & R. Anholt n
author keywords: Behavioral genetics; chemoreception; olfaction; proteomics; RNAi
MeSH headings : Animals; Behavior, Animal; Drosophila Proteins / genetics; Drosophila melanogaster / genetics; Gene Expression; Proteomics; Receptors, Odorant / genetics; Smell / genetics
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2011 journal article
Design, modeling, fabrication, and evaluation of the air amplifier for improved detection of biomolecules by electrospray ionization mass spectrometry
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 300(2-3), 99–107.
author keywords: Air amplifier; Electrospray ionization; Modeling; Detection limits
TL;DR:
A computer simulation that accurately portrays actual conditions and the results from these simulations are corroborated by the experimental data is developed, which can be used to predict outcomes from future designs resulting in a design process that is efficient in terms of financial cost and time.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
7. Affordable and Clean Energy
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2011 journal article
Performance Characteristics of a New Hybrid Quadrupole Time-of-Flight Tandem Mass Spectrometer (TripleTOF 5600)
ANALYTICAL CHEMISTRY, 83(13), 5442–5446.
MeSH headings : Fungal Proteins / metabolism; Proteome; Saccharomyces cerevisiae / metabolism; Tandem Mass Spectrometry / instrumentation; Trypsin / metabolism
TL;DR:
The TripleTOF 5600 System, a hybrid quadrupole time-of-flight mass spectrometer, was evaluated to explore the key figures of merit in generating peptide and protein identifications that included spectral acquisition rates, data quality, proteome coverage, and biological depth.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2011 article
John Bennett Fenn (1917-2010)
Muddiman, D. C. (2011, January 14). SCIENCE, Vol. 331, pp. 160–160.
MeSH headings : Chemistry / history; History, 20th Century; History, 21st Century; Nobel Prize; Spectrometry, Mass, Electrospray Ionization / history; United States
TL;DR:
The Nobel Prize in Chemistry did not define John Fenn, nor did it change him; he was the same man before and after, always showing a strong sense of humility.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2011 journal article
Thermodynamic, enzymatic and structural effects of removing a salt bridge at the base of loop 4 in (pro)caspase-3
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 508(1), 31–38.
author keywords: Apoptosis; Caspase activation; Enzyme activity; Protein folding and assembly
MeSH headings : Caspase 3 / chemistry; Caspase 3 / genetics; Caspase 3 / metabolism; Catalytic Domain; Crystallography, X-Ray; Enzyme Stability; Hydrogen Bonding; Models, Molecular; Mutation; Protein Folding; Static Electricity; Structure-Activity Relationship; Thermodynamics
TL;DR:
Results suggest E246 and K242 are important in procaspase-3 for their interaction with neighboring residues, not with one another, and formation of the K242-E246 salt bridge in caspases3 is needed for an accurate, stable conformation of loop L4 and proper active site formation in the mature enzyme.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
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