Works Published in 2020
Displaying works 1 - 20 of 30 in total
Sorted by most recent date added to the index first, which may not be the same as publication date order.
2020 journal article
Current status of the multinational Arabidopsis community
Plant Direct, 4(7).
author keywords: Arabidopsis thaliana; collaboration; Research Network; roadmap
TL;DR:
A call for ideas and foci for the “fourth decadal roadmap,” which will advise and coordinate the global activities of the Arabidopsis research community is initiated.
(via Semantic Scholar)
onlinelibrary.wiley.com (publisher PDF)
Source: ORCID
Added: April 1, 2022
2020 journal article
Complete Genome Sequences of Lactobacillus Strains C25 and P38, Isolated from Chicken Cecum
Microbiology Resource Announcements, 9(39).
Ed(s): D. Rasko
TL;DR:
The complete circular genome sequences of Lactobacillus crispatus strain C25, its plasmid, and LactOBacillus animalis strain P38; both strains were isolated from the cecum of 4-week-old chickens represent potential probiotic strains for poultry.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
15. Life on Land
(Web of Science)
Source: ORCID
Added: June 21, 2021
2020 journal article
Neutron Crystallography Data Collection and Processing for Modelling Hydrogen Atoms in Protein Structures
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 12(166).
MeSH headings : Amino Acids / chemistry; Buffers; Crystallization; Crystallography, X-Ray; Data Collection; Deuterium / chemistry; Hydrogen / chemistry; Hydrogen-Ion Concentration; Mixed Function Oxygenases / chemistry; Models, Molecular; Neurospora crassa / enzymology; Neutron Diffraction; Particle Size; Polysaccharides / chemistry; Proteins / chemistry; Signal-To-Noise Ratio; User-Computer Interface; Water / chemistry
TL;DR:
This work demonstrates mounting of hydrogenated protein crystals in quartz capillaries for neutron diffraction data collection, and the process of determining a neutron structure will be demonstrated using crystals of the lytic polysaccharide monooxygenase Neurospora crassa LPMO9D, a copper-containing metalloprotein involved in the degradation of recalcitrantpolysaccharides via oxidative cleavage of the glycosidic bond.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: June 10, 2021
2020 journal article
Caspases from scleractinian coral show unique regulatory features
JOURNAL OF BIOLOGICAL CHEMISTRY, 295(43), 14578–14591.
author keywords: caspase; cysteine protease; allosteric regulation; substrate specificity; coral apoptosis; coral immunity; functional divergence; substrate selection; CARD-caspase; apoptosis
MeSH headings : Amino Acid Sequence; Animals; Anthozoa / chemistry; Anthozoa / cytology; Anthozoa / enzymology; Anthozoa / metabolism; Apoptosis; Caspases / chemistry; Caspases / metabolism; Coral Reefs; Crystallography, X-Ray; Enzyme Activation; Humans; Models, Molecular; Protein Conformation; Protein Domains; Sequence Alignment; Substrate Specificity
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
14. Life Below Water
(OpenAlex)
Source: Web Of Science
Added: February 8, 2021
2020 journal article
A universal method for high-quality RNA extraction from plant tissues rich in starch, proteins and fiber
SCIENTIFIC REPORTS, 10(1).
MeSH headings : Dietary Fiber; Electrophoresis, Polyacrylamide Gel / methods; Plant Proteins; Plants / chemistry; Plants / genetics; RNA, Plant / isolation & purification; Seeds / chemistry; Seeds / genetics; Solid Phase Extraction / methods; Spectrophotometry; Starch
TL;DR:
The modified SDS-LiCl method is a robust and highly reproducible RNA extraction method for plant tissues rich in starch and other secondary metabolites and was demonstrated to be suitable for downstream applications, such as cDNA synthesis, gene amplification, and RT-qPCR.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: January 25, 2021
2020 journal article
Attenuated Salmonella enterica Serovar Typhimurium, Strain NC983, Is Immunogenic, and Protective against Virulent Typhimurium Challenges in Mice
VACCINES, 8(4).
author keywords: Salmonella typhimurium; NC983; vaccine; in vivo; infection; C57BL/6 mice; BALB/c mice; IgG
TL;DR:
Oral delivery of strain NC983 was capable of fully protecting C57BL/6 and BALB/c mice against challenge with virulent Typhimurium, and results indicate that strainNC983 is a potential live attenuated vaccine strain that warrants further development.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: January 19, 2021
2020 journal article
Deeply Sequenced Infectious Clones of Key Cassava Begomovirus Isolates from Cameroon
MICROBIOLOGY RESOURCE ANNOUNCEMENTS, 9(46).
TL;DR:
This work deeply sequenced two pairs of widely used infectious clones (4 plasmids) of the bipartite begomoviruses African cassava mosaic virus (ACMV) and East AfricanCassava mosaic Cameroon virus (EACMCV).
(via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: January 11, 2021
2020 journal article
Complete Genome Sequences of Six Lactobacilli Isolated from American Quarter Horses
MICROBIOLOGY RESOURCE ANNOUNCEMENTS, 9(47).
Ed(s): D. Rasko
TL;DR:
The complete circular genome sequences of six Lactobacillus strains and their plasmids, if any, from the fecal material of quarter horses at different ages are reported.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: January 4, 2021
2020 journal article
Metabolomic Profile of Allantoic and Amniotic Fluid in Late-term Gestational Mares Characterized by H-1-nuclear Magnetic Resonance Spectroscopy
JOURNAL OF EQUINE VETERINARY SCIENCE, 94.
author keywords: Equine; Metabolomics; Allantoic; Amniotic; Nuclear magnetic resonance spectroscopy
MeSH headings : Allantois; Amniotic Fluid; Animals; Body Fluids; Female; Horses; Magnetic Resonance Spectroscopy; Pregnancy; Protons
TL;DR:
The goal of this study was to characterize the global metabolomic profile of equine allantoic and amniotic fluid through nuclear magnetic resonance spectroscopy offetal fluids from 12 pregnancies through ultrasound-guided transabdominal puncture.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: November 24, 2020
2020 journal article
A beginner’s guide to neutron macromolecular crystallography
The Biochemist, 42(6), 16–20.
Source: ORCID
Added: November 24, 2020
2020 journal article
Development of a structure-analysis pipeline using multiple-solvent crystal structures of barrier-to-autointegration factor
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 76, 1001–1014.
author keywords: BAF; multiple solvent crystal structures; conserved water for structure refinement; differentialB-factor analysis
MeSH headings : Binding Sites; DNA / metabolism; DNA-Binding Proteins / chemistry; DNA-Binding Proteins / metabolism; Dimerization; Humans; Ligands; Models, Molecular; Protein Binding; Solvents / chemistry
TL;DR:
An MSCS data-handling pipeline is presented that uses the Detection of Related Solvent Positions (DRoP) program to improve data quality, and normalized and differential B-factor analysis is shown to be a valuable tool to characterize the effects of specific solvents on defined regions of BAF.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Source: Web Of Science
Added: November 16, 2020
2020 review
Microbial Interactions Within Multiple-Strain Biological Control Agents Impact Soil-Borne Plant Disease
[Review of ]. FRONTIERS IN MICROBIOLOGY, 11.
author keywords: microbial interaction; biological control agents; soil-borne disease; consortia; microbiome and community
TL;DR:
The current state of the use of single strain and multi-strain biological control consortia in the management of soil-borne diseases and potential mechanisms used by microbial components to improve the disease suppressing efficacy are summarized.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
Source: Web Of Science
Added: November 16, 2020
2020 journal article
Expression, purification, and phylogenetic analysis of MDIS1-INTERACTING RECEPTOR-LIKE KINASE1 (MIK1)
PROTEIN JOURNAL, 39(5), 461–471.
author keywords: LRR-RLK; Post-translational modifications; N-glycosylation; LC– MS; Phylogenetic analysis
MeSH headings : Arabidopsis / enzymology; Arabidopsis / genetics; Arabidopsis Proteins / biosynthesis; Arabidopsis Proteins / chemistry; Arabidopsis Proteins / genetics; Arabidopsis Proteins / isolation & purification; Gene Expression; Phylogeny; Protein Kinases / biosynthesis; Protein Kinases / chemistry; Protein Kinases / genetics; Protein Kinases / isolation & purification; Recombinant Proteins / biosynthesis; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / isolation & purification
TL;DR:
This work describes expression and purification of a recombinant plant LRR-RLK ectodomain MIK1 using a modified baculovirus-mediated expression system with subsequent N-linked glycosylation analysis using LC–MS/MS and computational sequence-based analyses.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Source: Web Of Science
Added: November 16, 2020
2020 journal article
Comparing DNA replication programs reveals large timing shifts at centromeres of endocycling cells in maize roots
PLOS GENETICS, 16(10).
MeSH headings : Cell Nucleus / drug effects; Cell Nucleus / genetics; Centromere / drug effects; Centromere / genetics; DNA Replication / drug effects; DNA Replication / genetics; DNA Replication Timing / drug effects; DNA Replication Timing / genetics; DNA, Plant / drug effects; DNA, Plant / genetics; Deoxyuridine / analogs & derivatives; Deoxyuridine / pharmacology; Endocytosis / drug effects; Meristem / drug effects; Meristem / genetics; Mitosis / drug effects; Mitosis / genetics; Nucleosomes / drug effects; Plant Roots / drug effects; Plant Roots / genetics; Plant Roots / growth & development; S Phase / genetics; Zea mays / genetics; Zea mays / growth & development
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: November 9, 2020
2020 article
Exploring the Rap1A Active Site Through Accelerated Molecular Dynamic Simulations
Schwabe, M., Holzapfel, G., & Mattos, C. (2020, April). FASEB JOURNAL, Vol. 34.
UN Sustainable Development Goal Categories
8. Decent Work and Economic Growth
(OpenAlex)
Source: Web Of Science
Added: October 12, 2020
2020 journal article
Quantitative proteomic analysis of the tizoxanide effect in vero cells
SCIENTIFIC REPORTS, 10(1).
MeSH headings : Animals; Anti-Infective Agents / pharmacology; Chlorocebus aethiops; Fatty Acid Synthases / genetics; Fatty Acid Synthases / metabolism; Proteome / drug effects; Proteome / genetics; Proteome / metabolism; Ribonucleoproteins / genetics; Ribonucleoproteins / metabolism; Thiazoles / pharmacology; Vero Cells
TL;DR:
The broad antimicrobial range of TIZ points towards its overall effect in lowering cell metabolism and RNA processing and modification, and the decreased levels of FASN, HNRNPH and HNRNPK with the treatment appear to be important for antiviral activity.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: October 5, 2020
2020 journal article
The impact of Zika virus infection on human semen: a case study
FUTURE VIROLOGY, 15(1), 5–12.
author keywords: assisted reproduction; Flavivirus persistence; immunofluorescence; longitudinal study; semen; semen donor; sexually transmitted virus; super-resolution microscopy; virus clearance; Zika virus
TL;DR:
A significant decrease in sperm concentration and in the percentage of progressive motility of sperm was observed, but the complete clearance of ZIKV from semen was not achieved.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: September 7, 2020
2020 review
Geminivirus Resistance: A Minireview
[Review of ]. FRONTIERS IN PLANT SCIENCE, 11.
author keywords: geminivirus; resistance; genetic resistance; agriculture; genetic engineering
TL;DR:
This review will focus on the generation of plants that are resistant to geminiviruses themselves, and on the methods used to control the insect vector using pesticides or genetic insect resistance.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Source: Web Of Science
Added: August 31, 2020
2020 article
Dynamic nuclear polarization enhanced neutron crystallography: Amplifying hydrogen in biological crystals
NEUTRON CRYSTALLOGRAPHY IN STRUCTURAL BIOLOGY, Vol. 634, pp. 153–175.
MeSH headings : Crystallography; Hydrogen; Neutron Diffraction; Neutrons; Protons
TL;DR:
This chapter describes current efforts to develop and apply DNP techniques for spin polarized neutron protein crystallography, highlighting concepts, experimental design, labeling strategies and recent results, as well as considering new strategies for data collection and analysis that these techniques could enable.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 24, 2020
2020 article
IMAGINE: The neutron protein crystallography beamline at the high flux isotope reactor
NEUTRON CRYSTALLOGRAPHY IN STRUCTURAL BIOLOGY, Vol. 634, pp. 69–85.
MeSH headings : Crystallography; Crystallography, X-Ray; Neutron Diffraction; Neutrons; Proteins; Synchrotrons
TL;DR:
The beamline components, the diffractometer and the data collection and data analysis protocols that are used are described, and the protein deuteration, crystallization and conventional crystallography capabilities that are available to users at ORNL's neutron facilities are outlined.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 24, 2020
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